Abstract: This study was carried out to determine the seroprevalence of bovine leptospirosis in K... more Abstract: This study was carried out to determine the seroprevalence of bovine leptospirosis in Kayseri, Turkey and to detect the leptospires in blood and urine of clinically suspect animals by serologic and molecular methods. A total of 2395 blood samples were collected from ...
Objective: Leptospirosis is one of the most common zoonotic diseases worldwide. Transmission to h... more Objective: Leptospirosis is one of the most common zoonotic diseases worldwide. Transmission to humans usually occurs by mucosal inoculation of the bacteria or exposure of non-intact skin via contact with water or soil contaminated with urine or feces of rodents. Occupational groups such as farmers, rice plant workers, sewer workers, miners, hunters, military personnel, mariners, shepherds, milkers, slaughterhouse workers, veterinarians and laboratory personnel are under risk for leptospirosis. In this study, we aimed to determine the seroprevalence of leptospirosis in coal miners in Zonguldak region. Methods: Blood samples were obtained from total 185 volunteer coal miners, who worked in 5 coal mines of Turkish Hard Coal Authority in Zonguldak and Bartın provinces, during June and July 2013. Serum samples were stored at -80°C. Additionally, we conducted an interview with each miner about demographical and epidemiological data such as age, location, duration of mining, other occupations, observing rodent or rodent excreta in the mine, noticing rodent contact with personal belongings or drinks or food in mine and hand hygiene compliance. Microscopic agglutination test (MAT), the reference diagnostic test for seroepidemiological studies of leptospirosis, was conducted in Spirochetal Diseases Diagnostic Laboratory, Veterinary Control Central Research Institute, Etlik, Ankara in June 2014. Anti-Leptospira antibody levels for 8 serotypes (Leptospira grippotyphosa Moskva V, L. australis Bratislava Jez Bratislava, L. canicola Hund Utrech IV, L. hardjo Hardjoprajitno, Özet Amaç: Leptospiroz, dünya çapında en yaygın görülen zoonotik hastalıklardan birisidir. İnsanlara çoğunlukla fare dışkısı ve idrarıyla kontamine olan su ve toprakla temas sonucu mikroorganizmanın bütünlüğü bozulmuş deriden veya mukozadan vücuda girmesiyle bulaşır. Çiftçiler, çeltik işinde çalışanlar, kanalizasyon işçileri, maden işçileri, avcılar, askeri personel, gemiciler, çobanlar, süt sağıcıları, mezbaha işçileri, kasaplar, veteriner hekimler ve laboratuvar çalışanları leptospiroz açısından riskli meslek gruplarıdır. Bu çalışmada Zonguldak bölgesinde kömür madeni işçilerinde leptospiroz seroprevalansının belirlenmesi amaçlanmıştır. Yöntemler: Zonguldak ve Bartın illerindeki Türkiye Taşkömürü Kurumu’na bağlı 5 farklı kömür madeninde çalışan toplam 185 gönüllü maden işçisinden, 2013 Haziran-Temmuz aylarında kan örnekleri alınmıştır. Serum örnekleri -80°C’de saklanmıştır. Ayrıca maden işçilerinin yaş, yerleşim bölgesi, madende çalışma süresi, başka mesleklerle uğraşma, madende fare ve/veya fare pisliği görme, madende farelerin eşya ve/veya yiyecek içecekle temasını tespit etme ve madende el hijyeni gibi demografik ve epidemiyolojik verileri için anket yapılmıştır. Leptospiroz seroepidemiyolojik çalışmalarında referans test yöntemi olarak kullanılan mikroskopik aglütinasyon testi (MAT) Ankara Etlik Veteriner Kontrol Merkez Araştırma Enstitüsü Spiroket Hastalıkları Teşhis Laboratuvarı’nda Haziran 2014’te yapılmıştır. MAT ile 8 ayrı serotip için (Leptospira grippotyphosa Moskva V, L. australis Bratislava Jez Bratislava, L. canicola Hund Utrech IV, L. hardjo Hardjoprajitno, L. pomona Pomona, L. icterohaemorrÖzgün Araştırma / Original Article
A PCR-based denaturing-gradient gel electrophoresis (DGGE) approach was applied to a partial sequ... more A PCR-based denaturing-gradient gel electrophoresis (DGGE) approach was applied to a partial sequence of the β- d -glucuronidase gene ( uidA ) for specific detection and differentiation of Escherichia coli populations according to their uidA sequence variations. Detection of sequence variations by PCR-DGGE and by PCR with direct sequencing correlated perfectly. Screening of 50 E. coli freshwater isolates and reference strains revealed 11 sequence types, showing nine polymorphic sites and an average number of pairwise differences between alleles of the uidA gene fragments (screened fragment length, 126 bp) of 2.3%. Among the analyzed strains a range of dominating to more rarely and/or uniquely observed E. coli sequence types was revealed. PCR-DGGE applied to fecally polluted river water samples simultaneously detected E. coli and generated a fingerprint of the mixed populations by separating the polymorphic uidA amplicons. No significant differences between non-cultivation-based and ...
Abstract: This study was carried out to determine the seroprevalence of bovine leptospirosis in K... more Abstract: This study was carried out to determine the seroprevalence of bovine leptospirosis in Kayseri, Turkey and to detect the leptospires in blood and urine of clinically suspect animals by serologic and molecular methods. A total of 2395 blood samples were collected from ...
Objective: Leptospirosis is one of the most common zoonotic diseases worldwide. Transmission to h... more Objective: Leptospirosis is one of the most common zoonotic diseases worldwide. Transmission to humans usually occurs by mucosal inoculation of the bacteria or exposure of non-intact skin via contact with water or soil contaminated with urine or feces of rodents. Occupational groups such as farmers, rice plant workers, sewer workers, miners, hunters, military personnel, mariners, shepherds, milkers, slaughterhouse workers, veterinarians and laboratory personnel are under risk for leptospirosis. In this study, we aimed to determine the seroprevalence of leptospirosis in coal miners in Zonguldak region. Methods: Blood samples were obtained from total 185 volunteer coal miners, who worked in 5 coal mines of Turkish Hard Coal Authority in Zonguldak and Bartın provinces, during June and July 2013. Serum samples were stored at -80°C. Additionally, we conducted an interview with each miner about demographical and epidemiological data such as age, location, duration of mining, other occupations, observing rodent or rodent excreta in the mine, noticing rodent contact with personal belongings or drinks or food in mine and hand hygiene compliance. Microscopic agglutination test (MAT), the reference diagnostic test for seroepidemiological studies of leptospirosis, was conducted in Spirochetal Diseases Diagnostic Laboratory, Veterinary Control Central Research Institute, Etlik, Ankara in June 2014. Anti-Leptospira antibody levels for 8 serotypes (Leptospira grippotyphosa Moskva V, L. australis Bratislava Jez Bratislava, L. canicola Hund Utrech IV, L. hardjo Hardjoprajitno, Özet Amaç: Leptospiroz, dünya çapında en yaygın görülen zoonotik hastalıklardan birisidir. İnsanlara çoğunlukla fare dışkısı ve idrarıyla kontamine olan su ve toprakla temas sonucu mikroorganizmanın bütünlüğü bozulmuş deriden veya mukozadan vücuda girmesiyle bulaşır. Çiftçiler, çeltik işinde çalışanlar, kanalizasyon işçileri, maden işçileri, avcılar, askeri personel, gemiciler, çobanlar, süt sağıcıları, mezbaha işçileri, kasaplar, veteriner hekimler ve laboratuvar çalışanları leptospiroz açısından riskli meslek gruplarıdır. Bu çalışmada Zonguldak bölgesinde kömür madeni işçilerinde leptospiroz seroprevalansının belirlenmesi amaçlanmıştır. Yöntemler: Zonguldak ve Bartın illerindeki Türkiye Taşkömürü Kurumu’na bağlı 5 farklı kömür madeninde çalışan toplam 185 gönüllü maden işçisinden, 2013 Haziran-Temmuz aylarında kan örnekleri alınmıştır. Serum örnekleri -80°C’de saklanmıştır. Ayrıca maden işçilerinin yaş, yerleşim bölgesi, madende çalışma süresi, başka mesleklerle uğraşma, madende fare ve/veya fare pisliği görme, madende farelerin eşya ve/veya yiyecek içecekle temasını tespit etme ve madende el hijyeni gibi demografik ve epidemiyolojik verileri için anket yapılmıştır. Leptospiroz seroepidemiyolojik çalışmalarında referans test yöntemi olarak kullanılan mikroskopik aglütinasyon testi (MAT) Ankara Etlik Veteriner Kontrol Merkez Araştırma Enstitüsü Spiroket Hastalıkları Teşhis Laboratuvarı’nda Haziran 2014’te yapılmıştır. MAT ile 8 ayrı serotip için (Leptospira grippotyphosa Moskva V, L. australis Bratislava Jez Bratislava, L. canicola Hund Utrech IV, L. hardjo Hardjoprajitno, L. pomona Pomona, L. icterohaemorrÖzgün Araştırma / Original Article
A PCR-based denaturing-gradient gel electrophoresis (DGGE) approach was applied to a partial sequ... more A PCR-based denaturing-gradient gel electrophoresis (DGGE) approach was applied to a partial sequence of the β- d -glucuronidase gene ( uidA ) for specific detection and differentiation of Escherichia coli populations according to their uidA sequence variations. Detection of sequence variations by PCR-DGGE and by PCR with direct sequencing correlated perfectly. Screening of 50 E. coli freshwater isolates and reference strains revealed 11 sequence types, showing nine polymorphic sites and an average number of pairwise differences between alleles of the uidA gene fragments (screened fragment length, 126 bp) of 2.3%. Among the analyzed strains a range of dominating to more rarely and/or uniquely observed E. coli sequence types was revealed. PCR-DGGE applied to fecally polluted river water samples simultaneously detected E. coli and generated a fingerprint of the mixed populations by separating the polymorphic uidA amplicons. No significant differences between non-cultivation-based and ...
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Papers by Erdinç Atabek