Papers by Eli-Judy Schmell
Journal of Biological Chemistry, Aug 1, 1981
Journal of Biological Chemistry, Mar 1, 1982
Journal of Biological Chemistry, Nov 1, 1979
Journal of Biological Chemistry, 1978
Rat hepatocytes, isolated by a collagenase perfusion technique, specifically bind to polyacrylami... more Rat hepatocytes, isolated by a collagenase perfusion technique, specifically bind to polyacrylamide gels containing covalently immobilized Gaminohexyl fin -galactopyranosyl groups. Less than 5% of these cells bind to polyacrylamide or to gels with the following covalently linked ligands: 6aminohexanol, or the 6-aminohexyl o-pyranosides of cu-mannose, @glucose, P-Z-acetamido-2-deoxyglucose, p-cellobiose, p-maltose, or P-melibiose. Cell binding to /3-o-galactoside gels occurs after a lag period at 37" and 65 to 100% (depending on the cell preparation) of the cells adhere. The duration of the lag period is inversely related to the /3-ogalactoside content of the gel but preincubation of the cells at 37" reduces the lag period. Cell-gel binding is a threshold phenomenon. Adhesion of cells to gels does not occur when the glycoside concentration is less than about 900 nmol per cm* x 0.25 mm thick gel piece. Above this critical concentration, cell-gel binding occurs and becomes maximal when the concentration is increased by only 20%. If these in vitro results apply to cellular interactions in uiuo, they suggest that slight changes in the levels of cell surface or extracellular matrix carbohydrates may profoundly influence the behavior of neighboring cells.
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Papers by Eli-Judy Schmell