Papers by Eleojo Ojogbane
Asian Journal of Research in Medical and Pharmaceutical Sciences, 2019
Aim: The aim of the study is to evaluate the bioactive compounds, toxicity and anti-bacterial act... more Aim: The aim of the study is to evaluate the bioactive compounds, toxicity and anti-bacterial activities of leaf extract of Vernonia amygdalina. Methodology: The phytochemical analysis of Vernonia amygdalina to detect the presence of bioactive compounds (oxalate, tannins, saponins, flavonoid, cardiac glycoside, alkaloids, steroid, balsams, essential oil and saponin glycoside) was performed using standard methods. The antibacterial activity of the leaf extracts was determined using agar well diffusion method against clinical isolates of Salmonella typhi and Salmonella paratyphi. The toxicity testing was carried out with albino rats using standard method. Results: Different secondary metabolites were found to be present in the leaf extracts after the phytochemical screening. They include tannins, saponins, flavonoids, cardiac glycosides, alkaloids, glycosides, steroid, saponin glycoside, volatile oil and Balsams. Highest zone of inhibition of 10.0 mm and 20.0 mm was recorded against S...
International Journal of Scientific Reports, 2021
Background: Induction of toxicity using nitrosamines provides a reliable animal model for the stu... more Background: Induction of toxicity using nitrosamines provides a reliable animal model for the study of oxidative damage to lipids, cellular membranes, proteins and DNA. In the present report, the effects of partially purified fractions of Senna occidentalis leaves on diethylnitrosamine intoxicated rats were studied.Methods: Fractions obtained from eluting the column with solvents of increasing polarity, n-hexane, chloroform, ethyl acetate, ethanol, methanol and distilled water were subjected to in vitro for their ability to scavenge 1, 1-dipheny l, 2-pycryl hydrazyl (DPPH) radical. Fraction 6a eluted with ethyl acetate:ethanol (50:50) possessed the highest antioxidant activity, this fraction was therefore selected for in vivo studies. Twenty rats, each weighing between 150 to 250 g were randomly allocated into four groups of five rats each. Hepatotoxicity was induced using a single intraperitoneal injection of diethylnitrosamine (DEN) at the 200 mg/kg body weight. Treatment was carr...
Cyphostemma glaucophilla is used in the treatment of kwashiorkor in ethno-medicine in Kogi and Kw... more Cyphostemma glaucophilla is used in the treatment of kwashiorkor in ethno-medicine in Kogi and Kwara States of Nigeria. The aqueous extract of pulverised dried leaves of C.glaucophilla, administered at various concentrations on some micro organism were evaluated in cultured plates of nutrient Agar. Three different antibiotics; ampiclox, lincomycin and chloramphenicol were used as synthetic drugs to evaluate their comparative efficacy with the extract. Five Groups A, B, C, D and E of rats weighing 100-110g of either sex were served with rat feed and water (control, A). Kwashiorkor was induced in Groups B, C, D and E by given protein deficient diet(corn meal) for 14 days. After 14 days, the malnourished Groups were served oral daily doses of 0.5, 1.0, 1.5 and 2.0 mg/kg b.w of the extract for 14 days respectively while Group A recieved (0.85g NaCl; 5ml/kg). The blood samples were obtained via occular puncture into heparinised centrifuge tubes and spun at 1000rpm for five minutes to obtain the plasma on day 0 (start of treatment) and day 14 (end of treatment) for the determination of the concentration of total protein and albumin by standard methods. Data were analysed by one way ANOVA and student independent t-test and presented as mean ± standard deviation values of p < 0.05 were considered significant. Cyphostemma glaucophilla leaves extract exhibited anti-microbial properties comparable with the antibiotics and produced dose dependent significant (p < 0.05) increases in the concentration of plasma total protiens and albumin in kwashiorkor induced rats.
Journal of Pharmacology and Toxicology, 2020
Journal of Ethnopharmacology, 2006
This present study was undertaken to evaluate the anti-ulcerogenic and anti-inflammatory properti... more This present study was undertaken to evaluate the anti-ulcerogenic and anti-inflammatory properties of methanol extract from the leaves of Cissus multistriata. Gastric ulcer and inflammation were induced in experimental animals using ethanol and acetic acid respectively. Anti-ulcerogenic action of C. multistriata was evaluated in albino rats at doses of 30, 50 and 100 mg/kg body weight. Cissus multistriata showed gastro-protective action in the groups and the protection was dose dependent and comparable to the standard drug (Omeprazole) used. The mechanism involved in the gastro-protective effect of C. multistriata may be related to the mucosal protector factors, such as Nitric Oxide (NO) and Sulfhydryl (SH) compounds. Anti-inflammatory activity was also studied on the plant using the paw edema method on different groups of rats. The methanol extract of C. multistriata exhibited potent anti-inflammatory activity at 150 mg/kg b.w at 4 h after administration to group 4 when compared with reference standard drug (Indomethacin). Group 2 had 72% inhibition of edema as compared to 55% inhibition offered by the extract at 150 mg/kg, thus showing a great effect at higher doses. From this study, it is clear that C. multistriata leaf extract had significant anti-ulcer activity in animal model. It also demonstrated high antiinflammatory potential at 150 mg/kg b.w. These observed pharmacological activities therefore, provide scientific support for the ethno-medicinal uses of this plant.
The effects of aqueous extract of pulverised dried leaves of Cyphostemma glaucophilla was investi... more The effects of aqueous extract of pulverised dried leaves of Cyphostemma glaucophilla was investigated in two sets of experiment. Thirty Rattus norvegicus of either sex, aged 7-9months and weighing 100-130g were randomly assigned to fiv e experimental group, A, B, C, D, and E of six animals each and were served single intraper itoneal daily doses of saline t tracycline was not significant [0.06±0.02mg/dL]. However, inhibiti on with Chloramphenicol and streptomycin was significant [4.60±0.02, 4.50±0. (0.85percent, Nacl.5ml/Kg],0.5,1.0,1.5,2.0mg/Kg of extract respectively for 14days.Twenty four hours after the last administration, the animals were sacrificed by ether anaesthesia and blood samples w ere drawn into heparinised tubes by cardiac puncture. The livers were excised. Protein concentr ations of both plasma and liver homogenate were assayed. In experiment two, extract induced in crease in protein concentration were inhibited with drugs that inhibit the different sta ges of prot...
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Papers by Eleojo Ojogbane