Papers by Edmundo Bonilla
Medical Science Research, Oct 29, 1994
Reproductive Toxicology, Nov 1, 2010
Journal of Applied Toxicology, Aug 7, 2020
Biochemical Pharmacology, May 1, 2003
Doxorubicin is an effective anticancer drug but its use is limited due to its adverse side effect... more Doxorubicin is an effective anticancer drug but its use is limited due to its adverse side effects such as infertility and cardiomyopathy. Some possible mechanisms of the action of doxorubicin have been postulated, but the initial gene deregulation response has not been investigated. Fetal life stages are critical periods in mammalian oogenesis. This study analyzes gene expression alterations in mouse fetal oocytes exposed in vitro to this anticancer agent. cDNA libraries were generated from isolated fetal oocytes and differential screenings performed with cDNAs from in vitro doxorubicin-treated and -untreated oocytes. Differentially expressed genes were assessed by real-time RT-PCR to quantify the extent of their transcriptional control in doxorubicin-exposed oocytes. The results show that doxorubicin alters the expression of genes involved in the mitochondrial respiratory chain, intracellular transport and cell differentiation. Finally, the up-regulation of a differentially expressed gene (metaxin) mediated by its promoter was evaluated in a functional assay. When treated with doxorubicin, somatic cells transfected with a genetic construct including the promoter of metaxin and a reporter gene showed increases in expression similar to those observed in fetal oocytes. This demonstrates the direct effect of agent on the regulation of a specific gene.
Molecular human reproduction, Jan 18, 2008
Porcine Health Management, Oct 18, 2021
DOAJ (DOAJ: Directory of Open Access Journals), Jul 1, 2018
Oocyte maturation involves several complex events which are partially regulated by the gonadotrop... more Oocyte maturation involves several complex events which are partially regulated by the gonadotropins. However, in order to fully understand these events it is necessary to identify all those genes whith roles in maturation. One of these genes is the protein ATPase6, which is a component of ATP synthase and is upregulated during oocyte maturation, nevertheless, its participation has not been experimentally evaluated. Therefore, the aim of the present study was to determine if ATPase6 has a relevant role in pig oocyte maturation, by conforming two groups of oocytes: The experimental, oocytes microinjected with ATPase6-interfering RNA (RNAi) in order to silence its expression, and the control, oocytes microinjected with an invertebrate gene-RNAi. After microinjection, both groups of oocytes were cultured in maturation medium for 48 h. ATPase6 expression was reduced 71% in ATPase6-RNAi microinjected oocytes, while the percentage of matured oocytes was reduced 51% in this group (67.5% in control oocytes vs 33% in ATPase6-RNAi microinjected oocytes; p<0.05 with Student’s t test). These results allow us to conclude that ATPase6 has an important role in the maturation of pig oocytes.
Zygote, May 1, 1998
This list suggests multiple ligands on the sperm surface (Myles & Primakoff, 1997), and might inc... more This list suggests multiple ligands on the sperm surface (Myles & Primakoff, 1997), and might include ligands for initial contact, subsequent binding, and fusion (Green, 1993). Antibodies to fertilin, M29 and M37, and DE inhibited fusion, but not binding. These data suggest these antigens are not involved in initial gamete contact, although they might be involved in a subsequent gamete binding step prior to fusion. Sperm PM proteins demonstrate affinity for oocyte PM proteins on western ligand blots (Ash et al., 1995). Although this interaction suggests the involvement of the identified proteins in the binding or fusion process, affinities under more psychological conditions need to be verified. Gamete binding during the fertilisation process begins with the interactions between complementary molecules in the zona pellucida (ZP) and the sperm plasma membrane (PM). After the sperm has passed through the ZP, sperm-oocyte binding and fusion occurs. The molecular events occurring at the level of the sperm and oocyte PM have yet to be fully understood. Potential mammalian sperm ligands for the oocyte PM include fertilin, the M29, M37, OBF 13 antigens and
Toxicology in Vitro, Feb 1, 2018
Previous studies with perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) indic... more Previous studies with perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) indicate that they act as endocrine disruptors, in addition to inducing alterations and damaging reproductive health; however, the biological mechanisms by which these disorders are produced are not yet understood. The aim of this study was to analyze the effect of PFOS and PFOA on in vitro steroidogenic secretion in porcine theca and granulosa cells, with or without gonadotropic stimulation. Granulosa and theca cells were isolated and cultured. Cell nature was performed by immunocytochemistry. PFOS and PFOA effect on steroid secretion was analyzed by chemiluminescence. In the present study, alterations in steroidogenic secretion were found when administering PFOS (0.12, 1.2, 12, 120 or 240 μM) or PFOA (0.012, 0.12, 1.2, 12 or 24 μM) to theca and granulosa cells. When theca and granulosa cells were stimulated with 500 ng/mL LH or 500 ng/mL FHS, respectively and immediately followed with 1.2 μM of PFOS or PFOA, the perfluorinated compounds inhibited the secretion of steroid hormones in both stimulated cell types. The results indicate that PFOS and PFOA act on steroidogenic ovarian cells as endocrine disruptors, which could affect the dependent functions of sexual steroids.
Systems Biology in Reproductive Medicine, Feb 27, 2023
Advances in Experimental Medicine and Biology, 1998
In previous presentations we have seen that individuals are exposed to several environmental phys... more In previous presentations we have seen that individuals are exposed to several environmental physical and chemical compounds with many tissues as target. For us main target are the germ cells. In many cases, the interaction of these compounds with the biological systems can produce genetic effects like dominant lethal mutations, aneuploidy, heritable translocations or reproductive effects as infertility and sterility (Barlow and Sullivan, 1982; Wyrobek, 1993; Altamirano et al., 1996) (Figure 1).
Theriogenology, Aug 1, 1994
The aim of the present study was to determine whether the previous addition of porcine zona pellu... more The aim of the present study was to determine whether the previous addition of porcine zona pellucida (ZP) components to spermatozoa of the same species has an inhibitory effect on in vitro fertilization (IVF). Boar spermatozoa were exposed to whole porcine solubilized zona pellucida (SZP), ZP glycoproteins (55 kDa and 90 kDa) and peptides (37 kDa, 40 kDa and 68kDa). Doses tested were 40, 70 and 100 ug/ml. In vitro fertilization was clearly inhibited by each component when the oocytes were compared with those fertilized with untreated spermatozoa. All the components had an effect in a dose dependent manner.
Toxicology in Vitro, 2019
Effect of perfluorodecanoic acid on pig oocyte viability, intracellular calcium levels and gap ju... more Effect of perfluorodecanoic acid on pig oocyte viability, intracellular calcium levels and gap junction intercellular communication during oocyte maturation in vitro, Toxicology in Vitro,
Zygote, Feb 15, 2013
SummaryIn a previous study, we have identified a set of conserved spermatogenic genes whose expre... more SummaryIn a previous study, we have identified a set of conserved spermatogenic genes whose expression is restricted to testis and ovary and that are developmentally regulated. One of these genes, the transcription factorMael, has been reported to play an essential role in mouse spermatogenesis. Nevertheless, the role ofMaelin mouse oogenesis has not been defined. In order to analyse the role ofMaelin mouse oogenesis, the expression of this gene was blocked during early oogenesis in mousein vitrousing RNAi technology. In addition, the role ofMaelduring differentiation of embryonic stem cells (ESC) into germ cellsin vitrowas analysed. Results show that downregulation ofMaelby a specific short interfering RNA disrupted fetal oocyte growth and differentiation in fetal ovary explants in culture and the expression of several germ-cell markers in ESC during their differentiation. These results suggest that there is an important role forMaelin early oogenesis and during germ-cell differentiation from embryonic stem cells in mousein vitro.
Medical Science Research, 1997
Gynecological Endocrinology, 2019
Turner syndrome (TS) is a common genetic disorder. TS-phenotype includes short stature, gonadal d... more Turner syndrome (TS) is a common genetic disorder. TS-phenotype includes short stature, gonadal dysgenesis, cardiac and kidney malformations, low bone mineral density (low-BMD) and thyroiditis. TSphenotype varies from patient to patient and the cause is not clear, the genomic background may be an important contributor for this variability. Our aim was to identify the association of specific single nucleotide variants in the PTPN22, VDR, KL, and CYP27B1 genes and vitamin D-metabolism, heart malformation, renal malformation, thyroiditis, and low-BMD in 61 Mexican TS-patients. DNA samples were genotyped for SNVs: rs7975232 (VDR), rs9536282 (KL), rs4646536 (CYP27B1), and rs1599971 (PTPN22) using the KASP assay. Chi-square test under a recessive model and multifactorial dimensionality reduction method were used for analysis. We found a significant association between renal malformation and the rs9536282 (KL) variant and between rs4646536 (CYP27B1) and low-BMD, these variants may have modest effects on these characteristics but contribute to the variability of the TS phenotype. In addition, we identified gene-gene interactions between variants in genes KL, CYP27B1 and VDR related to vitamin D-metabolism and low-BMD in TS-patients. Our results support the idea that the genetic background of TS-patients contributes to the clinical variability seen in them.
Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departam... more Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 29-06-200
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Papers by Edmundo Bonilla