Papers by Edikan Archibong
1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and Nort... more 1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA 2Division of Pharmacoengineering and Molecular Pharmaceutics and Center for Nanotechnology in Drug Delivery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA 3Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA 4These authors contributed equally to this work. *Corresponding author. Email: [email protected]
Dans un mode de realisation, une sonde de spectroscopie optique comprend une fibre optique ayant ... more Dans un mode de realisation, une sonde de spectroscopie optique comprend une fibre optique ayant une pointe distale et une chambre de filtration microfluidique fixee a la pointe distale de la fibre optique, la chambre comprenant une membrane microfluidique concue pour permettre au liquide d'entrer dans la chambre, tout en empechant les particules d'entrer dans la chambre.
Nature Biomedical Engineering, 2021
The immunosuppressive microenvironment of solid tumours reduces the antitumour activity of chimer... more The immunosuppressive microenvironment of solid tumours reduces the antitumour activity of chimeric antigen receptor T cells (CAR-T cells). Here, we show that the release-through the implantation of a hyaluronic acid hydrogel-of CAR-T cells targeting the human chondroitin sulfate proteoglycan 4, polymer nanoparticles encapsulating the cytokine interleukin-15 and platelets conjugated with the checkpoint inhibitor programmed death-ligand 1 into the tumour cavity of mice with a resected subcutaneous melanoma tumour inhibits the local recurrence of the tumour as well as the growth of distant tumours, through the abscopal effect. The hydrogel, which functions as a reservoir, facilitates the enhanced distribution of the CAR-T cells within the surgical bed, and the inflammatory microenvironment triggers platelet activation and the subsequent release of platelet-derived microparticles. The post-surgery local delivery of combination immunotherapy through a biocompatible hydrogel reservoir could represent a translational route for preventing the recurrence of cancers with resectable tumours.
National Science Review, 2021
Chimeric antigen receptor T cell (CAR T) therapy was a milestone in the treatment of relapsed and... more Chimeric antigen receptor T cell (CAR T) therapy was a milestone in the treatment of relapsed and refractory B cell malignancies. However, beneficial effects of CAR T cells have not been obtained in solid tumors yet. Herein, we implement a porous microneedle patch that accommodates CAR T cells and allows in situ penetration-mediated seeding of CAR T cells when implanted in the tumor bed or in the post-surgical resection cavity. CAR T cells loaded in the pores of the microneedle tips were readily escorted to the tumor in an evenly scattered manner without losing their activity. Such microneedle-mediated local delivery enhanced infiltration and immunostimulation of CAR T cells as compared to direct intratumoral injection. This tailorable patch offers a transformative platform for scattered seeding of living cells for treating a variety of tumors.
Science Advances, 2019
We developed an antigen delivery system based on the nanoerythrocyte vehicle combined with anti–P... more We developed an antigen delivery system based on the nanoerythrocyte vehicle combined with anti–PD-L1 for cancer immunotherapy.
Biomaterials, 2019
Tumor ablation therapies provide a minimally invasive approach to treat cancer. However, inhibiti... more Tumor ablation therapies provide a minimally invasive approach to treat cancer. However, inhibition of cancer metastasis and recurrence after ablation is still a challenge in clinical trials. Here, we propose a strategy using combinatorial thermal ablation, adjuvants and immune checkpoint blockade (ICB) to inhibit metastatic tumor and recurrence via antitumor immune responses post tumor thermal ablation, which are frequently used in the clinic. Furthermore, a strong immune memory against cancer was observed 80 days after the primary tumor was ablated. Considering that all components in our design are approved by Food and Drug Administration (FDA), we provide a strategy based on clinically used cancer treatment technique that is promising in clinical translation.
Biomaterials, 2019
Tumor ablation therapies provide a minimally invasive approach to treat cancer. However, inhibiti... more Tumor ablation therapies provide a minimally invasive approach to treat cancer. However, inhibition of cancer metastasis and recurrence after ablation is still a challenge in clinical trials. Here, we propose a strategy using combinatorial thermal ablation, adjuvants and immune checkpoint blockade (ICB) to inhibit metastatic tumor and recurrence via antitumor immune responses post tumor thermal ablation, which are frequently used in the clinic. Furthermore, a strong immune memory against cancer was observed 80 days after the primary tumor was ablated. Considering that all components in our design are approved by Food and Drug Administration (FDA), we provide a strategy based on clinically used cancer treatment technique that is promising in clinical translation.
Sensors and Actuators B: Chemical, 2019
Abstract Blood tests are a vital source of information for disease diagnosis and monitoring. Bloo... more Abstract Blood tests are a vital source of information for disease diagnosis and monitoring. Blood sample contamination due to hemolysis (the rupturing of red blood cells) is a significant problem that can result in incorrect diagnoses, delayed treatment, and inappropriate prescriptions. Therefore, a simple, rapid test is needed to detect the occurrence of hemolysis. Here we propose a new miniature spectroscopic device, integrated onto an optical fiber platform, which can quickly and reliably determine the amount of free hemoglobin in a small droplet of blood plasma. Optimal design parameters for the device were first calculated theoretically, and then the device was fabricated and tested. Experimental tests demonstrated that the device can quickly measure free plasma hemoglobin over a wide range of concentration (0–500 mg/dL) and with a low detection limit (
Materials Letters, 2018
Abstract Nanoscale metal composite materials with tunable properties have attracted interest in m... more Abstract Nanoscale metal composite materials with tunable properties have attracted interest in many areas, such as catalysis, electronics, and photography. Typical matrices include zeolites, metal oxides, or polymer films. This study reports for first time the incorporation of a dinuclear copper (II) complex in polyethylene oxide (PEO) fiber mat by electrospinning. EPR studies have shown that the complex remained intact and stable within the polymer fibers. The fiber morphology was characterized by SEM. The absorption of the complex allowed to monitor the composition and distribution of the metal on the material.
Nature chemical biology, 2018
Generating artificial pancreatic beta cells by using synthetic materials to mimic glucose-respons... more Generating artificial pancreatic beta cells by using synthetic materials to mimic glucose-responsive insulin secretion in a robust manner holds promise for improving clinical outcomes in people with diabetes. Here, we describe the construction of artificial beta cells (AβCs) with a multicompartmental 'vesicles-in-vesicle' superstructure equipped with a glucose-metabolism system and membrane-fusion machinery. Through a sequential cascade of glucose uptake, enzymatic oxidation and proton efflux, the AβCs can effectively distinguish between high and normal glucose levels. Under hyperglycemic conditions, high glucose uptake and oxidation generate a low pH (<5.6), which then induces steric deshielding of peptides tethered to the insulin-loaded inner small liposomal vesicles. The peptides on the small vesicles then form coiled coils with the complementary peptides anchored on the inner surfaces of large vesicles, thus bringing the membranes of the inner and outer vesicles toget...
Journal of Electronic Packaging, 2016
We propose a new approach to the modular packaging of microfluidic components, in which different... more We propose a new approach to the modular packaging of microfluidic components, in which different functional components are not only fabricated separately but are also designed to be individually removable for the purposes of replacement or subsequent analysis. In this paper, we demonstrate one such component: a stand-alone microfluidic filter that can be custom-fabricated and then connected, disconnected, and replaced on a microfluidic chip as needed. This filter is also designed such that particles captured on the filter can be further analyzed or processed directly on the filter itself—for example, for microscopic examination or cell culturing. The filter is a thin (1 μm) transparent silicon nitride membrane that can be designed and fabricated according to specifications for different applications. This material is suitable for microscale fabrication; filtration of a variety of solutions, including biological samples; and subsequent particle imaging and processing. The porous nat...
Advanced Biosystems, 2017
Jinqiang Wang received his Ph.D. degree from Zhejiang University in 2013, under the guidance of P... more Jinqiang Wang received his Ph.D. degree from Zhejiang University in 2013, under the guidance of Prof. Youqing Shen in the Department of Chemical Engineering. He is currently a postdoctoral associate working with Prof. Zhen Gu in the Joint Department of Biomedical Engineering at University of North Carolina at Chapel Hill and North Carolina State University. His research interests include development of new materials and systems for both cancer drug and insulin delivery.
Materials Letters, 2018
A new Schiff base type of polyester containing 2,2-dimethyl-1,3-diaminopropane was prepared by so... more A new Schiff base type of polyester containing 2,2-dimethyl-1,3-diaminopropane was prepared by solution polycondensation of 1,4-benzenedicarbonyl dichloride with Bis(4-hydroxybenzilaldehid)-2,2-dimethyl-1,3-propildiimine (H 2 HB 2 P) which is derived from a 2,2-dimethyl-1,3-diaminopropane Schiff base reacted with a 4-hydroxybenzaldehyde monomer. The monomer and the polyester were characterized by FTIR, 1 HNMR, and elemental analysis. The prepared polyester showed inherent viscosity of 0.29 dl/g in NMP at 25 °C, indicating their moderate molecular weight. The Polyester was completely soluble in aprotic polar solvents such as N-methylpyrolidone (NMP), dimethylformamide (DMF), Dimethyl Acetamid (DMAC), dimthylsulfoxide (DMSO). TGA determined the 10% weight loss temperature (T 10) at 280 °C and residual weight at 600 °C ca. 41% under nitrogen atmosphere.
Biosensors and Bioelectronics, 2017
Preeclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelet count) syndrome are p... more Preeclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelet count) syndrome are pregnancy-related complications with high rates of morbidity and mortality. HELLP syndrome, in particular, can be difficult to diagnose. Recent work suggests that elevated levels of free cell hemoglobin in blood plasma can, as early as the first trimester, potentially serve as a diagnostic biomarker for impending complications. We therefore developed a pointof-care mobile phone-based platform that can quickly characterize a patient's level of hemolysis by measuring the color of of blood plasma. The custom hardware and software are designed to be easy to use. A sample of the whole blood (~10 μL or less) is first collected into a clear capillary tube or microtube, which is then inserted into a low-cost 3D-printed sample holder attached to the phone. A 5-10 min period of quiescence allows for gravitational sedimentation of the red blood cells, leaving a layer of yellowish plasma at the top of the tube. The phone camera then photographs the capillary tube and analyzes the color components of the cell-free plasma layer. The software converts these color values to a concentration of free hemoglobin, based on a built-in calibration curve, and reports the patient's hemolysis level: non-hemolyzed, slightly hemolyzed, mildly hemolyzed, frankly hemolyzed, or grossly hemolyzed. . The accuracy of the method is ~1 mg dL-1. This phone-based point-of-care system provides the potentially life
Sensing and Bio-Sensing Research, 2015
Administering a wrong drug or a wrong dose can be extremely dangerous and can result in severe ad... more Administering a wrong drug or a wrong dose can be extremely dangerous and can result in severe adverse effects or even the death of a patient. With human errors being possible, automatic real time identification of a drug and its concentration using technology is a viable option to decrease the chance of incorrect drug administration. As a step toward this goal, we propose a new optical fiber based spectroscopic system that has built-in filtration capabilities and thus can work in real time near patient without additional sample pre-processing. It is designed as a point probe consisting of an optical fiber with a miniature filtering reflector integrated on the interface. In the future it can be inserted into a bag for intravenous therapy (IV therapy) or in a syringe to measure the spectrum of the fluid and to confirm its properties. Additionally, use of microfluidic filtration allows to remove microscopic particles from the sample and thus decreases the noise and increases the sensitivity of spectroscopic measurement. In this study, an optofluidic system was fabricated, and filtration capabilities and measurement of cobalamin (vitamin B 12) concentration have been demonstrated.
International Journal of Chemistry, 2011
The present study investigates the binding properties of four dansyl substituted aza-crown ethers... more The present study investigates the binding properties of four dansyl substituted aza-crown ethers with alkali, alkaline earth metal ions and ammonium. The influence of the solvent polarity and protonation on the photophysical properties of the compounds was studied by UV/Vis and fluorescence methods. The host species caused only slight changes on the absorption spectra of the ligands. The fluorescence changes were more pronounced and concentration dependent thus allowing to calculate the binding constants of the process. The most stable complex under our working conditions was the one between Ba 2+ and DNS18C6.
Synthetic Metals, 2012
Polyaniline in form of emeraldine salt and emeraldine base was used as a matrix to attach several... more Polyaniline in form of emeraldine salt and emeraldine base was used as a matrix to attach several labeled and non-labeled dioxin selective pentapeptides both directly to the polymer and using glutaraldehyde as a linker. The peptides have been selected as a model to study the binding process due to their smaller size, lower sensitivity to the environment and potential application as solid state extraction reagents for chlorinated toxins. The composition and the properties of the compounds were investigated by means of elemental analysis, XPS, FTIR, UV/vis, and fluorescence spectroscopy. The results have shown that 3.30-7.76% peptides were attached to the emeraldine base both with and without a linker. Glutaraldehyde and the peptides were connected to the matrix via chemical bond resulting in formation of compounds whit similar composition and stability in a broad pH range. The influence of the linker and the peptides on the electronic properties and composition of the polymer have been investigated by principal component analysis.
Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2010
Three novel aza-crown ether derivatives incorporating 4-amino-7-nitrobenzaoxa-1,3-diazole (NBD) c... more Three novel aza-crown ether derivatives incorporating 4-amino-7-nitrobenzaoxa-1,3-diazole (NBD) chromophore were synthesized and their structure confirmed by 1 H-NMR, IR and elemental analysis. The influence of the solvent polarity and protonation on the photophysical properties of NBD-15-crown-5 was studied by UV/Vis and fluorescence methods. The influence of the investigated cations on the absorption spectra of the ligands was negligible, however emission was strongly affected. Complexation and binding stability of NBD-aza-15-crown-5 and NBD-aza-18-crown-6 were studied using fluorescence spectroscopy. NBD-aza-18-crown-6 exhibits strong selectivity toward Ca 2+ and Sr 2+ ions with formation constants about 10 3 times higher than the formation constants with the other ions included in the study.
Chemical Engineering Journal, 2013
h i g h l i g h t s " TCMCs were synthesized by graft copolymerization for the first time. " The ... more h i g h l i g h t s " TCMCs were synthesized by graft copolymerization for the first time. " The processing property of TCMC was characterized. " Electrospinning of TCMC was explored and nanofibers were obtained. " The results showed that TCMC exhibited thermoplastic property and spinnability. g r a p h i c a l a b s t r a c t New thermoplastic carboxymethyl cellulose (TCMC) was synthesized by graft copolymerization. The main objective of this study was to investigate the thermoplastic and spinnability of thermoplastic carboxymethyl cellulose (TCMC). We found that TCMC exhibited thermoplastic property, and nanofibers with diameters from 250 nm to about 4 lm were obtained through electrospinning.
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Papers by Edikan Archibong