Papers by Dra. Nuria E. Rocha Guzmán
Food Chemistry, 2009
Water infusions of mature and fresh Quercus resinosa leaves were evaluated for antioxidant activi... more Water infusions of mature and fresh Quercus resinosa leaves were evaluated for antioxidant activity and genotoxic effects on HeLa cells. Native Mexicans used to drink Q. resinosa leaves tea as a refreshing beverage. The air dried leaves were pulverised and boiled in water, then their phenolic content and condensed tannins were determined. The chromatographic profile of 15 phenolic components in Quercus leaves infusions was also determined by HPLC. In vitro analysis of antioxidant capacity of leaves infusion extracts were performed by the DPPH method and the deoxyribose assay. The genotoxicity of Q. resinosa leaves extracts was evaluated on HeLa cells as well as its underlying mechanism by the single-cell electrophoresis assay (comet assay). Results show that fresh leaves infusions increase the oxidative process and other damage to DNA in transformed human cells. Fresh leaves from Q. resinosa may serve as a potential source of phenolics with anticancer activity.
Starch and Starchy Food Products, Jul 26, 2022
Journal of Food Processing and Preservation, 2022
Revista Chapingo Serie Ciencias Forestales y del Ambiente, 2021
Introduction: Beyond the commercial value of coffee grains there is recent interest for the non-t... more Introduction: Beyond the commercial value of coffee grains there is recent interest for the non-timber benefit and traditional medicinal properties of the Coffea tree leaves.Objective: To determine the phytochemical composition, antioxidant properties and inhibition of oxidative stress by extracts from Coffea leaves obtained by ultrasound assistance as an opportunity for production of plant bioactives. Material and methods: The phytochemical composition of ten extracts of Coffea arabica L. leaves cv. Catimor 5000 was evaluated in their phenolic and methylxanthines contents as well their antioxidant activity in vitro through nitric oxide (NO), absorbance capacity of oxygen radicals (ORAC), ferric reducing antioxidant power (FRAP), and radical ABTS (2,2'-azino-bis [3-ethylbenzothiazoline-6-sulfonic acid]) assays, and their effect on inhibition of oxidative stress induced by hydrogen peroxide in human colon adenocarcinoma cells (HT-29). Extracts were obtained by ultrasound assistan...
Food and Bioproducts Processing, 2021
Abstract In this study, we carried out an exhaustive characterization of the polyphenol profile o... more Abstract In this study, we carried out an exhaustive characterization of the polyphenol profile of strawberry, raspberry, blueberry, and blackberry decoctions; as well as the extractable (EPP) and hydrolysable (HPP) polyphenolic and proanthocyanidin profile of the residues obtained during berries decoctions (decoction by-products) by UPLC-ESI-Q-ToF MS. A greater variety of polyphenols was identified in the EPP fraction of the berry decoction by-products as compared to the decoctions, which were rich in several ellagitannins, flavonols, hydroxybenzoic acids, and anthocyanins. Ellagic acid was identified as the major polyphenol after acid hydrolysis in all berry decoction by-products, followed by hydroxybenzoic acid, which was found bound to the matrix by glycosidic, ether and ester bonds. Regarding proanthocyanidins, procyanidin, prodelphinidin, and propelargonidin dimers were identified in the strawberry, raspberry, and blackberry decoction by-products by MALDI-ToF MS. The results obtained from this study demonstrates that berries decoctions are rich in polyphenols; however, berry decoction by-products were richer in polyphenols with a great variety of extractable and non-extractable polyphenols.
Food Chemistry, 2021
Instant controlled pressure drop (DIC) has been used as a pre-treatment to increase extractable p... more Instant controlled pressure drop (DIC) has been used as a pre-treatment to increase extractable polyphenols (EPP), mainly attributed to matrix structure expansion. This work aimed to evaluate the effect of DIC on non-extractable polyphenols (NEPP), EPP, and dietary fiber on grape pomace. At 0.2 MPa-60 s was observe an increase of total EPP and total anthocyanins. Despite the increment of EPP, was observe the lowest anthocyanins and non-extractable proanthocyanidins content at 0.4 MPa-120 s. This increase was due to a partial transformation of anthocyanins into phenolic acids and the depolymerization of proanthocyanidins. Also was observe partial solubilization of insoluble dietary fiber. Morphologically, the size of the pores generated by DIC was more significant at higher pressures. Thus, DIC modified the morphology and profile of the polyphenols of grape pomace, producing phenolic compounds of simpler structure and improving their antioxidant capacities.
SN Applied Sciences, 2020
The objective of the present investigation was to evaluate the physical stability of emulsions (w... more The objective of the present investigation was to evaluate the physical stability of emulsions (w/o) with a gel as an oil phase. Two oil phases (canola and coconut oil) were used to assess the impact of the different vegetable oils. Monoglycerides were used as the gelling agent and polyglycerol polyricinoleate as the surfactant. Micrographs, differential scanning calorimetry, and rheology tests were performed. The presence of crystalline structures was observed in the continuous phase characteristic of some organogels and a smaller distribution of sizes as a function of time. Also, a change in the crystallization profile of the aqueous and oily phases concerning to time was also found, the crystallization signals coinciding (≈ − 40 °C), indicating a better organization by the phases. No variability was found in modules G′ and G″, so these systems have good mechanical stability. The properties of the organogelated emulsions are explained by the interfaceinterface interactions present between the particles and the reduced mobility, which slows the phase separation.
Food & Function, 2020
Flavanols-rich cocoa reduced body fat composition in athletes through modulation of follistatin a... more Flavanols-rich cocoa reduced body fat composition in athletes through modulation of follistatin and leptin levels.
Bioprocess and Biosystems Engineering, 2020
The bioconversion process of bioactive naringenin by whole-cells of Yarrowia lipolytica 2.2ab for... more The bioconversion process of bioactive naringenin by whole-cells of Yarrowia lipolytica 2.2ab for the production of increased value-added compounds is successfully achieved in surface and liquid cultures. This approach is an alternative to the commercial production of these bioactive compounds from vegetable sources, which are limited due to their low concentrations and the complexity of the purification processes. The experimentation rendered seven value-added compounds in both surface and liquid bioconversion cultures. Some of the compounds produced have not been previously reported as products from the bioconversion processes, such as the case of ampelopsin. Biosynthetic pathways were suggested for the naringenin bioconversion using whole-cells of Y. lipolytica 2.2ab. Finally, the extracts obtained from the naringenin bioconversion in liquid cultures showed higher percentage of inhibition of DPPH• and ABTS• radicals up to 32.88 and 2.08 times, respectively, compared to commercial naringenin.
Medicinal Chemistry Research, 2019
The use of water, acetone, and hydroacetonic solutions as an extraction solvent for polyphenol co... more The use of water, acetone, and hydroacetonic solutions as an extraction solvent for polyphenol compounds from Buddleja scordioides has been investigated. The phenolic profiles determined by liquid chromatography-electrospray ionizationmass spectrometry/mass spectrometry (LC-ESI-MS/MS) were compared with those obtained with the traditional aqueous infusion used for the treatment of gastrointestinal disorders. It was evaluated the effects of polyphenol extract at physiologically relevant conditions against H 2 O 2-induced oxidative stress via attenuation of reactive oxygen species (ROS) levels and lipopolysaccharide (LPS)-induced inflammation in human HT-29 cells. The use of hydroacetonic solutions allows obtaining phytochemical extracts enriched with constituents that have antioxidant and anti-inflammatory activity in intestinal cells. This suggests that polyphenols of low and middle polarity from Buddleja scordioides may be better extracted with hydroacetonic solutions, and showing high nutraceutical potential to reduce oxidative stress associated with the onset and progress of inflammatory diseases.
Medicinal Chemistry Research, 2018
The aim of the study was to evaluate the effect of Buddleja scordioides Kunth leaves infusion on ... more The aim of the study was to evaluate the effect of Buddleja scordioides Kunth leaves infusion on lipid peroxidation in mice with ultraviolet (UV) light-induced oxidative stress. CD-1 mice were allocated into treatment and control groups (n = 8). Mice in the intervention group were treated with B. scordioides leaves infusion (500 µL equivalent to 0.6534 mg of lyophilized leave infusion/kg of body weight) while the controls received distilled water by gavage administration during 9 days. Both groups were UV irradiated (2.59320175 mW/cm 2) 4 consecutive days: 3 h per day with rest periods of 4 min every hour. B. scordioides leaves infusion had high concentrations of hydroxybenzoic acids, flavonols, hydroxycinnamic acids, and flavone compounds. Mice previously treated with B. scordioides leaves infusion exhibited a significant reduction on malondialdehyde (MDA) concentrations in the liver (77%) but not in kidney and plasma in comparison with the control group. B. scordioides leaves infusion shows hepatoprotective activity via free radical scavenging in mice with UV light-induced oxidative stress.
Journal of Food Processing and Preservation, 2019
Currently, western countries are characterized by changes in lifestyle associated to an increase ... more Currently, western countries are characterized by changes in lifestyle associated to an increase on the consumption of fast food and unhealthy snacks, with a consequent increase in the incidence of metabolic disorders. In this regard, there is a growing interest for functional foods which provides an additional benefit to health
Industrial Crops and Products, 2018
Moringa oleifera has an exceptional composition in nutrients and different medicinal compounds. P... more Moringa oleifera has an exceptional composition in nutrients and different medicinal compounds. Phenolic compounds present in Moringa plant could prevent chronic non-communicable diseases. An increase in the secondary metabolites of Moringa oleifera could improve its functional properties. One possible strategy to increase functional properties in plants is to cultivate them under fertilizer deficit regimes as elicitation treatment (i.e: nitrogen). The present study aimed to evaluate the effect of four nitrogen levels on the phenolic compounds profiles as well as on plant growth and functional properties in Moringa oleifera leaves. Total phenols and flavonoids, antioxidant and bactericidal activities of Moringa oleifera leaves extracts were evaluated. Our results displayed that reduce fertirrigation until 0.7 mg/L of N-NO3 (T0) had a positive impact on total phenols and flavonoids with 355 ± 4.9 μg CA/mg dry extract and 316 ± 8 μg Q/mg dry extract respectively, whereas the fertirrigation with 294.7 mg/L of N-NO3 (T100) It was found only 157 ± 8.1 μg CA/mg dry extract and 54 ± 0.1 μg Q/mg dry extract respectively. The higher values of phenolic content were improved the antioxidant activity and antibacterial properties of Moringa oleifera leaves extracts, nevertheless plant growth was affected.
Food Science and Biotechnology, 2019
The aim of this study was to evaluate the effect of agave fructans on short chain fatty acid (SCF... more The aim of this study was to evaluate the effect of agave fructans on short chain fatty acid (SCFA) production in mice (C57BL/6). Animals were randomly divided into three groups (n = 8): control group (CG), fructans from Agave tequilana group (FG) (55 g per day/kg of body weight), and oligrofructose group (OG) (55 g per day/kg of body weight). Treatments were administered oral via for 6 weeks. After 24 days of treatment (phase 1), both treated groups exhibited elevated levels of acetic (12%) and propionic (FG 66% and OG 38%) acids in comparison with the CG. After 42 days of treatment (phase 2), FG had higher concentrations of acetic (33%) and propionic (73%) acids than the CG; while OG exhibited higher levels of butyric (12%) and propionic (35%) acids compared with the CG. Fructans from Agave tequilana improve the production of SCFA in mice, suggesting a prebiotic activity.
Physiological and Molecular Plant Pathology, 2018
Hydrogen peroxide is an important signal molecule in plant defense against biotic and abiotic str... more Hydrogen peroxide is an important signal molecule in plant defense against biotic and abiotic stress. Pepper golden mosaic virus (PepGMV) is a whitefly-transmitted geminivirus causing significant yield and quality losses in pepper and other horticultural crops in Mexico. Several pesticides have been used trying to control whiteflies and thus, PepGMV disease in host crops. The present work examined the effect of exogenous application of hydrogen peroxide (H 2 O 2) on inducing resistance to PepGMV infection in pepper plants. Experiments were carried out under greenhouse conditions aiming to evaluate phenotypical, biochemical and molecular features in these pepper plants. Hydrogen peroxide at 6, 14 and 18 mM induced tolerance to PepGMV either by absence of symptoms as well as by attenuating and/or delaying them. The protection observed was directly dependent on the concentration of H 2 O 2 sprayed on plants. Moreover, PepGMV DNA levels were inversely proportional to the protection level. Enzymatic and gene expression profiles related with plant defense were induced in protected, in comparison to susceptible control plants. Interestingly, the levels of some phenolic compounds were also associated with plant protection. Taking together, these results suggested that exogenous foliar applications of H 2 O 2 protect pepper plants against PepGMV infection inducing the plant host defense arsenal.
European Journal of Lipid Science and Technology, 2018
This study aims to evaluate the effect of H. perforatum and S. cordifolia extracts on hepatic ste... more This study aims to evaluate the effect of H. perforatum and S. cordifolia extracts on hepatic steatosis in obese rats, and to elucidate their mechanisms through a lipidomic analysis. Fifty-seven phytochemical compounds are identified in H. perforatum and S. cordifolia aqueous extracts by UPLC-QTOF MS E. Both herbal aqueous extracts ameliorate hepatic steatosis. S. cordifolia modulates arachidonic acid, 16:0-, 16:1-, and 18:0-derived TAG, ceramides, and diacylglycerols, regulating the expression of genes involved in fatty acid (Fas, Acc, and Scd1), diacylglyceride (Gpat) and ceramide (Spt1) biosynthesis, and β-oxidation (Cpt1 and Acadm). H. perforatum regulates eicosapentanoic acid metabolism, which is associated with Fabp down-regulation. Both extracts reduce hepatic TNF-α and IL-6 levels, which is associated with eicosanoid pathway regulation by S. cordifolia and the modulation of phosphatidylcholines and phosphatidylethanolamines by H. perforatum. These results suggest that these aqueous extracts can be used as ingredients for the elaboration of functional beverages with hepatoprotective effects. Practical Applications: This study proposes a lipidomic approach followed by the integration of metabolic networks for the identification of the mechanisms associated with the hepatoprotective effect of herbal extracts. The results obtained in this study demonstrate the beneficial effect of S. cordifolia and H. perforatum aqueous extracts on hepatic steatosis in high-fat and fructose diet-fed obese rats. Therefore, these herbs can be used for the elaboration of functional beverages.
Food Research International, 2017
The aim of this study was to evaluate the anti-hyperglycemic and antioxidant effects of oak leave... more The aim of this study was to evaluate the anti-hyperglycemic and antioxidant effects of oak leaves infusions and fermented beverages from Quercus convallata and Q. arizonica in vitro and in vivo. Female C57BL/6 mice fed with high saturated fat and fructose diet-induced obesity were treated with oak leaves beverages (200 μL/per day equivalent to 15 mg of lyophilized sample/Kg of body weight for infusions and 31 mg of lyophilized sample/Kg of body weight for fermented beverages) for 3 months and an oral glucose tolerance test (OGTT) was performed. Blood plasma was obtained for determination of glucose, lipid profile, and oxidative stress markers (ABTS, nitric oxide, and ORAC assays). Insulin resistance was estimated using the product of triglycerides and glucose (TyG). Oak leaves infusions and fermented beverages exhibited exerted inhibition of α-amylase (8-15% and 5-9%, respectively) and α-glucosidase (98% and 99%, respectively) enzymes. After OGTT, the groups treated with either oak leaves infusions or fermented beverages showed lower glucose levels compared with the obesity control group (18%) and a similar glucose tolerance to healthy control group. On long-term evaluation, intervention groups showed a significant reduction in fasting glucose concentrations (41-50% for oak leaves infusions and 52-66% for fermented beverages) and TyG index (4.2-4.6% for oak leaves infusions and 5.9-7.5% for fermented beverages) compared with the obese control group. Oak leaves infusions and fermented beverages had antioxidant potential in vitro and scavenging activity for radicals such as peroxyl and peroxynitrite anions. Our results suggest anti-hyperglycemic and antioxidant effects of beverages prepared with leaves of Quercus species in vitro and in vivo.
Food Chemistry, 2016
The aim of this study was to determine the effect of chitosan (CH), salicylic acid (SA) and hydro... more The aim of this study was to determine the effect of chitosan (CH), salicylic acid (SA) and hydrogen peroxide (H2O2) at different concentrations on the antinutritional and nutraceutical content, as well as the antioxidant capacity of bean sprouts (cv Dalia). All elicitors at medium and high concentrations reduced the antinutritional content of lectins (48%), trypsin inhibitor (57%), amylase inhibitor (49%) and phytic acid (56%). Sprouts treated with CH, SA and H2O2 (7 µM; 1 and 2 mM, and 30 mM respectively) increased the content of phenolic compounds (1.8-fold), total flavonoids (3-fold), saponins (1.8fold) and antioxidant capacity (37%). Furthermore, the UPLC-ESI-MS/MS analysis showed an increase of several nutraceutical compounds in bean sprouts treated with SA such as coumaric (8.5-fold), salicylic (115-fold), gallic (25-fold) and caffeic (1.7-fold) acids, as well as epigallocathechin (63-fold), rutin (41-fold) and quercetin (16.6-fold) 2 flavonoids. The application of elicitors in bean seed during sprouting enhances their nutraceutical properties.
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Papers by Dra. Nuria E. Rocha Guzmán