Papers by Dr.Sateesh Chauhan
European Journal of Pharmaceutics and Biopharmaceutics, Jun 1, 2010
p-Hydroxybenzoic acid esters (parabens) are commonly used antimicrobial preservatives in pharmace... more p-Hydroxybenzoic acid esters (parabens) are commonly used antimicrobial preservatives in pharmaceutical formulations. Two microorganisms, isolated from non-sterile methyl paraben (MP) and propyl paraben (PP) solutions, were found to degrade the respective parabens. Identification by 16S rRNA partial gene sequencing revealed them to be Pseudomonas beteli and Burkholderia latens, respectively. The present work describes a previously unreported interaction of the parabens with P. beteli and B. latens. Degradation of MP at various concentrations by P. beteli, followed a logarithmic pattern, while that of PP by B. latens was found to be linear. It was subsequently observed that P. beteli could degrade only MP, while B. latens could degrade both the parabens. Absence of HPLC chromatogram peaks of expected degradation products indicated that the parabens were used up as a carbon source. The behaviour of pathogens (Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Aspergillus niger) of the pharmacopoeial preservative effectiveness test (PET), towards MP, showed that none had the ability to degrade the paraben. It was concluded that, for a paraben-preserved multi-dose ophthalmic formulation, the sole use of the four pathogens that are recommended by the pharmacopoeia for PET can falsely indicate the formulation to be effective against 'in-use' contamination.
Indian Journal of Pharmaceutical Sciences, 2002
Simple and reproducible HPTLC and HPLC methods for the determination of pistacienoic acids in Pis... more Simple and reproducible HPTLC and HPLC methods for the determination of pistacienoic acids in Pistacia integerrima were developed and are described. The HPTLC method involves separation of components by TLC on precoated Silica gel 60 F254 plate with a solvent system of chloroform: methanol (95:05) and detection at 220 nm in absorbance mode. The sensitivity of HPTLC method was found to be 1.0 µg and the linearity was observed in the range of 1.0 µg to 8.0 µg. The HPLC method involves separation of pistacienoic acids using mobile phase comprising of acetonitrile:water:phosphoric acid (80:20:1) and scanning the chromatogram at 220 nm using a photodiode array detector. The response was linear in the range of 1.25 µg to 10.0 µg. The proposed methods being precise and sensitive can be used for detection, monitoring and quantification of pistacienoic acids in P. integerrima.
Indian Journal of Pharmaceutical Sciences, 1998
A simple reproducible HPTLC method for the determination of glycyrrhizin in Glycyrrhiza glabra an... more A simple reproducible HPTLC method for the determination of glycyrrhizin in Glycyrrhiza glabra and its extract was developed and is described. The sensitivity was found to be linear in the range of 0.2 to 1.0 I¼g. The proposed method being precise, sensitive and reproducible can be used for detection, monitoring and quantification of glycyrrhizin in g. glabra and its extract.
Journal of Planar Chromatography – Modern TLC, 2007
International Journal of Basic & Clinical Pharmacology, 2020
Background: Benzalkonium chloride (BKC) is the most used preservative in topical eye drops but it... more Background: Benzalkonium chloride (BKC) is the most used preservative in topical eye drops but it causes effects such as dry eye and trabecular meshwork degeneration. Polyhexamethylene biguanide (PHMB) is a polymeric biguanide is a less harmful preservative used in ophthalmic solutions. The objective of this study to com-pare the efficacy of PHMB preserved versus BKC preserved ophthalmic solutions containing brimonidine tartrate and timolol maleate on intraocular pressure (IOP) following single ocular instillation in New Zealand white (NZW) rabbits.Methods: This study was conducted on 12 normotensive male NZW rabbits (2.9-3.6 kg) between 6-9 months of age. Animals received single ocular instillation of 35 µl ophthalmic solution containing brimonidine tartrate 0.15 %w/v and timolol maleate 0.5% w/v with PHMB as preservative (n=6, test) or BKC as preservative (n=6, reference) as per the randomization. Intraocular pressure (IOP) was measured before and 2, 4, 6, 8 and 24 hours after ins...
La presente invention concerne une composition parenterale stable d'un complexe de vitamines ... more La presente invention concerne une composition parenterale stable d'un complexe de vitamines B et de chlorhydrate de lidocaine qui est stabilisee par une combinaison synergetique de stabilisants tels que l'acide DL-malique, l'edetate disodique et l'hexacyanoferrate de potassium III ; et le procede s'y rapportant.
L'invention se refere a une composition ophtalmique stable et efficace pour application topiq... more L'invention se refere a une composition ophtalmique stable et efficace pour application topique qui comprend un agent mydriatique et un polymere viscoelastique, et a des procedes de preparation d'une telle composition destinee a une usage humain et veterinaire.
Pharmaceutical Development and Technology, 2010
The present work studies the interaction of methyl paraben (MPB) and propyl paraben (PPB), two wi... more The present work studies the interaction of methyl paraben (MPB) and propyl paraben (PPB), two widely used antimicrobial agents in multi-dose ophthalmic formulations, with 5 mL, low density polyethylene (LDPE) and polypropylene (PP) blow-fill-seal (BFS) packs, by subjecting the systems to accelerated stability conditions of 40°C/25% RH. The effect of pH, paraben concentration, and relative humidity (RH) on the sorption loss of both the parabens was studied. Additionally, the effects of buffer species and buffer strength on MPB sorption were studied. LDPE packs showed significantly higher loss compared to PP packs which showed < 5% loss in all cases. PPB showed a significantly higher loss (40-50%) than MPB (9-16%) in LDPE. pH (3.0, 5.0, 7.0) did not have a statistically significant effect on sorption. However, concentration, humidity and buffer at pH 7 affected paraben sorption. The application of the power law suggested that the MPB followed non-Fickian diffusion while PPB showed non-Fickian to Case II diffusion in LDPE packs. In conclusion, caution should be exercised while using parabens in LDPE BFS packs because substantial losses of the antimicrobial agent during the shelf-life can compromise the preservative effectiveness against 'in-use' contamination.
Ancient science of life, 2004
A reverse phase High pressure liquid chromatography (HPLC) method to determine puerarin in Puerar... more A reverse phase High pressure liquid chromatography (HPLC) method to determine puerarin in Pueraria tuberose was developed and described. The method involves separation of active compound using mobile phase consisting of acetonitrile: water: orthophosporic acid in the ratio of 32:67:01 and detecting the chromatogram at 280 nm using photodiode array detector. The sensitivity of the method was observed as 1.0 mg and linearity was observed between 1.0 mg to 8.0mg. The puerarin content of 3.02% w/w was found in crude P.tuberosa The proposed method in precise, sensitive, reproducible and easy to perform which can be used for detection, monitoring and quantification of puerarin in P . tuberose.
Uploads
Papers by Dr.Sateesh Chauhan