Papers by Dr. Moni Krishno Mohanta
Journal of scientific research, May 1, 2023
Microbiology resource announcements, Sep 18, 2023
We report the complete genome sequences of four bacterial strains that were isolated from Blattel... more We report the complete genome sequences of four bacterial strains that were isolated from Blattella germanica (German cockroaches) that were found in three wards of the Rajshahi Medical College Hospital. Multiple antibiotic resistance genes were identified in each genome, with one genome containing multiple plasmid-encoded resistance genes.
Journal of advances in microbiology, Feb 18, 2023
Annual research & review in biology, Mar 4, 2020
Social Science Research Network, 2017
Journal of advances in microbiology, Jun 22, 2020
Aim: The aim of this study was undertaken for the isolation and molecular characterization of the... more Aim: The aim of this study was undertaken for the isolation and molecular characterization of the halophilic bacteria from salt affected soils. Place and Duration of Study: This study was conducted on the salinity affected area in Ramzan Nagor under Shamnagor Upazilla of Shatkhira District, Bangladesh. The collection of soil sample, isolation of halophilic bacteria and subsequent experiments were done from May to December 2018. Methodology: Salt affected soil could be good source of halophilic bacteria as it contain high amount of salt. Hence, one gram of salt affected soil sample which was containing high amount of salt was suspended to 100 ml sterile distilled water and one ml of sample from the top of the suspension were taken in to 250 ml Erlenmeyer flaks containing 100 ml of mineral salts (MS) medium. The primary enrichment was incubated for several days at 37C with shaking at 120 rpm (revolution per minute) on an orbital shaker. Cultures which were found to be turbid after a period of up to 4 days were used as inocula in subsequent experiments. Results: Four halophilic bacterial strains viz. Bacillus sp. strain 8-15, Enterobacter sp. strain LCR75, Acinetobacter sp. strain 407 and Acinetobacter junii strain F27 were isolated from the saline Original Research Article
Journal of Advances in Microbiology
The co-substrate is a crucial component in the bio-decolorization process that helps bacteria to ... more The co-substrate is a crucial component in the bio-decolorization process that helps bacteria to grow and carry out their fundamental functions. Different types of textile dyes with diverse chemical structures are frequently employed in the textile industry, and the most of them are resistant to biodegradation because of their xenobiotic nature. Due to the low carbon content of textile dyes, particularly of azo dyes, co-metabolic conditions are required for decolorization process. Hence, the current study was aimed to determine which striking carbon and nitrogen sources are preferred for generating co-metabolic conditions to decolorize the following dyes: Disperse Yellow-3, Malachite Green, Basic Red-18, and Reactive Blue-4. Different carbon and nitrogen sources were used to isolate, screen, and evaluate different bacterial strains for dye degradation. A potential bacterial strain Pseudomonas taiwanensis strain TNZ3 was identified based on 16s rDNA sequence and selected for further ...
Annual Research & Review in Biology
Discharge of textile industrial effluent without proper treatment has become a severe hazard for ... more Discharge of textile industrial effluent without proper treatment has become a severe hazard for the animal health and environment worldwide. Therefore, this study was designed to isolate azo dye-degrading bacteria from textile wastewater and evaluate their ability to biodegrade reactive dyes into non-toxic products. The potent bacterial strain which was isolated from textile wastewater was identified as Pseudomonas monteilii strain RZT1 on the basis of 16S rDNA sequence. The isolated bacterial strain exhibited good decolorization ability with yeast extract supplementation as cosubstrate in static conditions for Malachite Green dye. The optimal condition for the decolorization of Malachite Green dye by P. monteilii strain RZT1 were at pH 7.0 and 28°C. Decolorization rates of Malachite Green dye by P. monteilii strain RZT1 were varied with initial dye concentration as follow: 84.8%, 75.4%, 63.4% and 45.5% decolorization for 100ppm, 200ppm, 300ppm and 400ppm initial dye concentration ...
Annual Research & Review in Biology
Due to rapid industrialization and market demand of vibrant textile products, the natural textile... more Due to rapid industrialization and market demand of vibrant textile products, the natural textile dyes have been replaced by the synthetic textile dyes. These synthetic dyes are released in environment with textile wastewater resulting in a major environmental pollution, especially in aquatic ecosystem. Hence, aquatic organisms like fish are highly vulnerable to the pollution caused by dyes of textile wastewater. This study was designed to evaluate the deleterious effects of Basic Red-18 (BR-18) dye on behavior, survivability, haematology and histology of Tilapia fish (Tilapia mossambica) and to minimize these deleterious effects of BR-18 dye by bioremediation with the novel bacteria isolated from textile wastewater. The isolated novel bacteria was identified as Mangrovibacter yixingensis strain AKS2 by 16s rRNA sequencing (Accession no. OM189530). The 30% and 70% mortality rates were observed in fish exposed to commercial BR-18 dye at concentrations of 100 and 200 ppm respectively....
International Journal of Entomology Research, 2021
Rhizobium spp. was isolated from root nodules of lentil (Lens culinaris L.), and its morphologica... more Rhizobium spp. was isolated from root nodules of lentil (Lens culinaris L.), and its morphological, cultural, physiological and biochemical characteristics were studied. It was observed that the colonies were circular, light pink, convex, entire and opaque. The bacterium was gram negative, rod shaped, aerobic, non-spore forming and motile. Isolates were positive for Catalase, Citrate utilization test, Urea hydrolysis, Congored test, Nitrification test, Oxidase test, Triple sugar iron test, MacConkey agar test and, Motility tests. The samples were found negative for Methyl Red (MR), Voges-Proskauer(VP), Indole, Starch hydrolysis test, Hydrogen-sulfide production and Hofer’s alkaline test. The isolate can ferment sucrose, glucose, fructose, mannitol and produce acid. Screening for antibiotic resistance in this study revealed that the strain were resistant to Ampicilin, Erythromycin, Gentamicin, Amoxycillin, Penicillin, Streptomycin and Nalidixic acid. The isolate grew optimally at pH ...
American Scientific Research Journal for Engineering, Technology, and Sciences, 2017
The study was designed for isolation and characterization of azo dye decolourizing bacteria which... more The study was designed for isolation and characterization of azo dye decolourizing bacteria which is a prerequisite for developing a microorganism-facilitated treatment of polluting dyes. In this s ...
International Journal of Current Microbiology and Applied Sciences, 2018
fish parasites have also been signified as zoonotic and biological hazard in prospective to human... more fish parasites have also been signified as zoonotic and biological hazard in prospective to human health. Parasites are small organisms which feed on other animals, living in or on their skin or body slime, gill, intestine,
International Journal on Environmental Sciences, 2010
Extensive use of chromium, cadmium and lead nitrate in tanning industry has caused substantial en... more Extensive use of chromium, cadmium and lead nitrate in tanning industry has caused substantial environmental pollution in Bangladesh. Bioremediation of tanning effluents with the help of bacteria was investigated. Samples of effluents with cadmium, chromium and lead nitrate (10mg/ml) were incubated in mineral salt medium at 37°C for 4 days and bacterial strain was isolated from the sample. Preliminary characterization of the organisms according to Bergey's manual suggests that the organisms may belong to Coccus species. The rate of reduction of these effluents was determined by using the flame Atomic Absorption Spectrophotometer (AAS). Out of 3 ppm concentration the rates of reduction were found to be 2.96 ppm for chromium; 2.92 ppm for cadmium and 2.46 ppm for lead nitrate. The isolated bacteria harboured three endogenous plasmids. All of the plasmids were lost when the organisms were treated with ethidium bromide (100mg/ml). Loss of the plasmids resulted in disability of the b...
Imperial journal of interdisciplinary research, 2016
: In a series of experiments, pathogenic bacteria from poultry feeds and moribund hens’ reproduct... more : In a series of experiments, pathogenic bacteria from poultry feeds and moribund hens’ reproductive organs were isolated and identified, and susceptibility of the bacteria to a number of antibiotics was assessed. Four types of branded poultry feeds available in Rajshahi City Corporation areas viz., broiler starter (BS), broiler grower (BG), layer starter (LS) and layer layer (LL) were collected from five randomly chosen shops. The feed samples were cultured in nutrient agar media and the bacterial specimens were isolated and examined under microscope for their gross morphological and biochemical characteristics. Results showed that the bacterial isolates belonged to Escherichia coli, Enterococcus sp. and Salmonella sp. Total viable counts (TVC) of the bacteria were replicated five times per feed and the BS, BG, LS and LL showed 23.40±2.88×10 12 , 28.60±1.52×10 12 , 83.20±7.40×10 12 , and 33.20±1.30×10 12 cfumL -1 (colony forming units per millilitre), respectively. The highest TVC was recorded in LS where all three genera of the bacteria were found. In LL, on the other hand, E. coli and Salmonella sp. and in BS and BG, only Salmonella sp. was diagnosed. Sonali moribund hens were collected from five randomly selected poultry farms situated in Rajshahi metropolis. Samples of eggs, ovary and oviduct were suspended in physiological saline, one loopful of each suspension was inoculated separately into nutrient agar medium and the bacterial specimens were isolated and identified to be E. coli and Salmonella sp. TVC of bacteria in the eggs, ovary and oviduct samples yielded 100.40±3.96×10 12 , 72.20±3.35×10 12 and 70.20±3.96×10 12 cfumL -1 , respectively. The highest TVC was recorded in the eggs, eggs and ovary specimens only had Salmonella sp. whereas oviduct contained E. coli and Salmonella sp. Finally, antibiotic sensitivity patterns of the bacterial isolates were tested against eight commonly used antibiotics viz., ampicillin (AMP), bacitracin (BAC), ceftriaxone (CEF), ciprofloxacin (CIP), doxycycline (DOX), gentamicin (GEN), sulphamethoxazole (SUL) and tetracycline (TET). E. coli showed resistance to BAC and SUL, Enterococcus sp. was highly resistant only to SUL while Salmonella sp. showed high to moderate resistance to AMP, GEN and SUL. The presence of three pathogenic bacteria in poultry feeds in Rajshahi, coupled with the diagnosis of E. coli and Salmonella in the reproductive organs of the moribund hens were indicative of bacterial contamination which reinforces the need for effective control measures against the pathogenic microbes, due care in handling of the poultry feeds and proper hygiene in the processing of the poultry meat and eggs for human consumption.
Journal of entomology and zoology studies, 2020
Formalin, a carcinogenic agent, is illegally used for preservation of fruits and fishes in Bangla... more Formalin, a carcinogenic agent, is illegally used for preservation of fruits and fishes in Bangladesh. It is often observed that flies are avoiding some fruits and fishes for an unknown reason in fruits and fish markets. Therefore, it was assumed that flies may avoid the contaminated fruits and fishes by detection of presence of formalin in those items. Hence, this study was designed to uncover relationship between rate of avoidance of the fishes by flies and the concentration of formalin for contamination. For this study, five types of fruits viz. Mango, Orange, Apple, Papaya and Banana, and two types of fishes, viz. Puthi, Puntius titus (Hamilton, 1822) and Tilapia, Oreochromis niloticus were soaked with normal water (Control) and 120 ppm, 250ppm, 500ppm, 750ppm, 1000ppm and 2000ppm solution of formaldehyde for 1 minute. Then fruits and fishes were kept in different Petridis separately in open place for 1 hour to count the flies available on fruits and fishes. Finally, the counted...
Detection of resistance pattern of pathogens to frequently prescribed antibiotics is vital and su... more Detection of resistance pattern of pathogens to frequently prescribed antibiotics is vital and supportive in improving the quality of empirical treatment. Hence, the aim of this study was to explore the pattern of antibiotic resistance of E. coli isolated from patient with Urinary tract infections (UTIs). In this retrospective and observational study, 366 test reports of antibiotic sensitivity of bacterial isolates were used for data analyses which were collected from 9 districts of Bangladesh between June 2017 and August 2018 from the UTI patients. It was found that Escherichia coli (79.51%) was the most common causative agent in UTIs in both male and female patients. The females (>25 years old) and aged male (> 50 years) were more prone to UTIs than other gorups. Among the 34 tested antibiotics, only 4 antibiotics viz. Meropenem, Netilmicin, Imipenem and Amikacin were highly sensitive to E. coli. But, 25 of tested antibiotics were resistant or intermediate to more than 50% i...
Asian Journal of Fisheries and Aquatic Research, 2020
The probiotic bacteria isolated from prawn, Macrobrachium rosenbergii was studied for their morph... more The probiotic bacteria isolated from prawn, Macrobrachium rosenbergii was studied for their morphological and biochemical characteristics as well as their antagonistic efficacy against pathogenic bacteria. A total of three probiotic bacteria viz. White Colour Bacteria (WCB), Red Colour Bacteria (RCB) and Yellow Colour Bacteria (YCB) were isolated from intestine of healthy prawn while three pathogenic bacteria viz. PB1, PB2 and PB3 were isolated from infected antennae and muscles of moribund prawn. Depending on their physical and biochemical features, the probiotic isolates were gram-positive, rod shaped and motile bacteria belonging to Bacillus spp. and the pathogenic bacteria were also identified as gram-negative, cocci shaped and motile bacteria fit in Enterococcus spp., Vibrio spp. and Micrococcus spp. The optimum culture conditions of all isolates were at pH 7.0 and 37°C temperature. Results on the antibiogram profile of pathogenic bacteria revealed that majority of the isolates...
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Papers by Dr. Moni Krishno Mohanta