Papers by Dr. Anshita Purohit
Drug and Chemical Toxicology, 2013
The present study was conducted to elucidate the protective effect of melatonin (MLT) and α-tocop... more The present study was conducted to elucidate the protective effect of melatonin (MLT) and α-tocopherol on mercury-induced genotoxicity in human blood cultures. The in vitro effects of inorganic mercury added to human lymphocytes on the cell-cycle proliferative index (CCPI)/proliferation replicative index (PRI) and sister chromatid exchange (SCE) using fluorescence plus Giemsa staining were examined. A significant increase occurred in SCE per metaphase (SCE/chromosome and SCE/cell) and inhibition of proliferative kinetics, which resulted in a decline of the replicative index, in comparison to the controls. Treated lymphocyte cultures also exhibited a reduction in %M1 and %M2 metaphase plates, but an increase in %M3 metaphase plates was noticed. Adding α-tocopherol and MLT individually and in combination indicated a mitigative effect by reducing the genotoxic potential of treated cultures. The percent amelioration for all the three parameters, namely, frequency of SCE, SCE/plate and SCE/chromosome as well as CCPI, was comparatively high with MLT and α-tocopherol in combination than MLT followed by α-tocopherol. The percent mitigation was better in combined antioxidant additions to toxicant-exposed cultures, compared to MLT, whereas the percent mitigation by α-tocopherol alone was less for average generation time and population doubling time, respectively.
Pharmacology & Pharmacy, 2011
Free radicals are common outcome of normal aerobic cellular metabolism. In-built antioxidant syst... more Free radicals are common outcome of normal aerobic cellular metabolism. In-built antioxidant system of body plays its decisive role in prevention of any loss due to free radicals. However, imbalanced defense mechanism of antioxidants and overproduction or incorporation of free radicals from environment to living systems leads to serious damage. It also attacks nervous system resulting in neural-degeneration. In order to evaluate the neurotoxic effect on the brain parts of mercury in our study, oxidative stress indices of enzymatic and non enzymatic components were measured in rats intoxicated with mercury (2 mg and 4 mg/kg body weight) for 60 days to adult rats. Along with gravimetry, tissue burden was also recorded. Alterations in these indices were further supported by ultrastructural studies carried out in the brain as indicated by myelin disintegration, cell organelle alterations and neuronal loss by mercury poisoning. Treatment with the antioxidant melatonin (N-acetyl 5-methoxy tryptamine, 5 mg/kg) prevented mercury exerted toxicity due to its antioxidant property. The pathological changes were also ameliorated in the brain region comparatively to support biochemical profile of brain. Thus, melatonin produced neuroprotection against mercury poisoning in rats.
Abstract- The ability to perform high-throughput seed DNA extractions is highly desirable and ess... more Abstract- The ability to perform high-throughput seed DNA extractions is highly desirable and essential for plant breeding and other molecular screening techniques. DNA extraction methods for PCR-quality DNA from calluses and plants are not time efficient, since they require that the tissues be ground in liquid nitrogen, followed by precipitation of the DNA pellet in ethanol, washing and drying the pellet, etc. Secondly, it gets more difficult to isolate the DNA from seed containing oil. The need for a rapid and simple procedure is urgent, especially when hundreds of samples need to be analyzed. Hence, for this purpose, a simple and efficient method of isolating high-quality genomic DNA for PCR amplification and enzyme digestion from seed is developed.
Pollution is viewed as the release of substances and energy as waste product of human activities ... more Pollution is viewed as the release of substances and energy as waste product of human activities which result in harmful changes within the natural environment. In the present study water of Chandlodia lake was analyzed for various physico-chemcial parameters. The study was carried out for a period of one year. Montly data's been collected and were represented seasonally along with standard error. Different parameters taken in the study were Temperature, Electrical conductivity, Turbidity, Total dissolve solids, pH, Alkalinity, Total Hardness, Calcium, Magnesium, Dissolved Oxygen, Biochemcial oxygen demand, Chloride, Sodium, Nitrate and Phosphate. Therefore from the above result it was concluded that water of Chandlodia lake shows very high level of pollution.
The ability to perform high throughput seed DNA extractions is highly desirable and essential for... more The ability to perform high throughput seed DNA extractions is highly desirable and essential for plant breeding and other molecular screening techniques. DNA extraction methods for PCR quality DNA from calluses and plants are not time efficient, since they require that the tissues be ground in liquid nitrogen, followed by precipitation of the DNA pellet in ethanol, washing and drying the pellet, etc. Secondly, it gets more difficult to isolate the DNA from seed containing oil. The need for a rapid and simple procedure is urgent, especially when hundreds of samples need to be analyzed. Hence, for this purpose, a simple and efficient method of isolating high quality genomic DNA for PCR amplification and enzyme digestion from seed is developed.
Drug and Chemical Toxicology, 2010
The effect of melatonin on the neurotoxicity induced by mercuric chloride was studied. Adult rats... more The effect of melatonin on the neurotoxicity induced by mercuric chloride was studied. Adult rats were fed orally with two different doses of mercuric chloride (2 mg; 4 mg/kg body weight) to evaluate brain toxicity with respect to cerebral hemisphere, cerebellum, and medulla oblongata regions for 60 days with or without supplementation with melatonin (5 mg/kg body weight) intraperitoneally. The results suggest that the graded doses of mercury elicit the depletion of enzymatic activities, such as adenosine triphosphatase, succinate dehydrogenase, phosphorylase, alkaline phosphatase, acid phosphatase, altered glycogen, total protein, and lipid peroxidation levels in the cerebral hemisphere, cerebellum, and medulla oblongata of the brain, thereby affecting their respective functions. Blood glucose and mercury levels increased, followed by a reduction in body and organ weights. All these effects seemed to be severe in the cerebral hemisphere of the brain. Further affected indices were, to some extent, maintained in the brain of animals cotreated with melatonin, showing its protective role against mercury-exerted neurotoxicity.
International Journal of Scientific Research, 2012
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Papers by Dr. Anshita Purohit