Papers by Diana Ivette Aparicio Bautista
International journal of molecular sciences, Jul 9, 2024
Antioxidants, Feb 20, 2024
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of Zhejiang University-SCIENCE B
International Journal of Molecular Sciences
Epidemiological studies have reported that the Mexican population is highly susceptible to dyslip... more Epidemiological studies have reported that the Mexican population is highly susceptible to dyslipidemia. The MARC1, ADCY5, and BCO1 genes have recently been involved in lipidic abnormalities. This study aimed to analyze the association of single nucleotide polymorphisms (SNPs) rs2642438, rs56371916, and rs6564851 on MARC1, ADCY5, and BCO1 genes, respectively, with the lipid profile in a cohort of Mexican adults. We included 1900 Mexican adults from the Health Workers Cohort Study. Demographic and clinical data were collected through a structured questionnaire and standardized procedures. Genotyping was performed using a predesigned TaqMan assay. A genetic risk score (GRS) was created on the basis of the three genetic variants. Associations analysis was estimated using linear and logistic regression. Our results showed that rs2642438-A and rs6564851-A alleles had a risk association for hypertriglyceridemia (OR = 1.57, p = 0.013; and OR = 1.33, p = 0.031, respectively), and rs56371916...
Frontiers in endocrinology, Apr 23, 2024
Scientific Reports, 2022
End-point RT-PCR is a suitable alternative diagnostic technique since it is cheaper than RT-qPCR ... more End-point RT-PCR is a suitable alternative diagnostic technique since it is cheaper than RT-qPCR tests and can be implemented on a massive scale in low- and middle-income countries. In this work, a bioinformatic approach to guide the design of PCR primers was developed, and an alternative diagnostic test based on end-point PCR was designed. End-point PCR primers were designed through conservation analysis based on kmer frequency in SARS-CoV-2 and human respiratory pathogen genomes. Highly conserved regions were identified for primer design, and the resulting PCR primers were used to amplify 871 nasopharyngeal human samples with a previous RT-qPCR based SARS-CoV-2 diagnosis. The diagnostic test showed high accuracy in identifying SARS-CoV-2-positive samples including B.1.1.7, P.1, B.1.427/B.1.429 and B.1.617.2/ AY samples with a detection limit of 7.2 viral copies/µL. In addition, this test could discern SARS-CoV-2 infection from other viral infections with COVID-19-like symptomatolo...
International Journal of Infectious Diseases, 2021
The aim of this study was to investigate the feasibility of saliva sampling as a non-invasive and... more The aim of this study was to investigate the feasibility of saliva sampling as a non-invasive and safer tool to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and to compare its reproducibility and sensitivity with nasopharyngeal swab samples (NPS). The use of sample pools was also investigated. Methods: A total of 2107 paired samples were collected from asymptomatic healthcare and office workers in Mexico City. Sixty of these samples were also analyzed in two other independent laboratories for concordance analysis. Sample processing and analysis of virus genetic material were performed according to standard protocols described elsewhere. A pooling analysis was performed by analyzing the saliva pool and the individual pool components. Results: The concordance between NPS and saliva results was 95.2% (kappa 0.727, p = 0.0001) and 97.9% without considering inconclusive results (kappa 0.852, p = 0.0001). Saliva had a lower number of inconclusive results than NPS (0.9% vs 1.9%). Furthermore, saliva showed a significantly higher concentration of both total RNA and viral copies than NPS. Comparison of our results with those of the other two laboratories showed 100% and 97% concordance. Saliva samples are stable without the use of any preservative, and a positive SARS-CoV-2 sample can be detected 5, 10, and 15 days after collection when the sample is stored at 4 C. Conclusions: The study results indicate that saliva is as effective as NPS for the identification of SARS-CoV-2-infected asymptomatic patients. Sample pooling facilitates the analysis of a larger number of samples, with the benefit of cost reduction.
Journal of Zhejiang University-SCIENCE B, 2021
molitor larvae has shown both antiproliferative and proapoptotic properties against HCC and color... more molitor larvae has shown both antiproliferative and proapoptotic properties against HCC and colorectal adenocarcinoma in vitro (Wu et al., 2020). Such evidence proves that mealworms contain bioactive components with therapeutic properties, and suggests that the therapeutic potential of mealworms might be dependent on bioactive compounds synthesized at specific life stages of T. molitor development. This study aimed at exploring the therapeutic properties of aqueous extracts of three life stages of T. molitor, including larva, pupa, and adult, on the early hepatocarcinogenesis recapitulated by the chronic administration of diethylnitrosamine (DEN). First, individuals from each stage (Figs. 1a-1c) were periodically separated as described in supplementary materials and methods. Based on the highest solubility of each extract, pH 6.5, 6.0, and 7.0 were selected as the most efficient pH (Fig. 1d) to dissolve the biological materials extracted from larvae, pupae, and adults, respectively. The final concentrations were calculated based on previously reported doses and on the ad libitum daily water intake of mice (Materials and methods).
Basic & Clinical Pharmacology & Toxicology, 2020
Alcoholic liver disease (ALD) may be attributed to multiple hits driving several alterations. The... more Alcoholic liver disease (ALD) may be attributed to multiple hits driving several alterations. The aim of this work was to determine whether nucleoredoxin (NXN) interacts with flightless‐I (FLII)/actin complex and how this ternary complex is altered during ALD progression induced by different ALD models. ALD was recapitulated in C57BL/6J female mice by the well‐known ALD Lieber‐DeCarli model, and by an in vitro human co‐culture system overexpressing NXN. The effects of ethanol and low doses of lipopolysaccharides (LPS) and diethylnitrosamine (DEN) were also evaluated in vivo as a first approach of an ALD multi‐hit protocol. We demonstrated that NXN interacts with FLII/actin complex. This complex was differentially altered in ALD in vivo and in vitro, and NXN overexpression partially reverted this alteration. We also showed that ethanol, LPS and DEN synergistically induced liver structural disarrangement, steatosis and inflammatory infiltration accompanied by increased levels of proliferation (Ki67), ethanol metabolism (CYP2E1), hepatocarcinogenesis (GSTP1) and LPS‐inducible (MYD88 and TLR4) markers. In summary, we provide evidence showing that NXN/FLII/actin complex is involved in ALD progression and that NXN might be involved in the regulation of FLII/actin‐dependent cellular functions. Moreover, we present a promising first approach of a multi‐hit protocol to better recapitulate ALD pathogenesis.
Nutrients, 2019
Osteoporosis is a skeletal disease mainly affecting women over 50 years old and it represents a s... more Osteoporosis is a skeletal disease mainly affecting women over 50 years old and it represents a serious public health problem because of the high socioeconomic burden. This disease is characterized by deterioration of bone microarchitecture, low bone mineral density (BMD), and increased risk of fragility fractures. This study aimed to identify serum useful proteins as biomarkers for the diagnosis and/or prognosis of osteoporosis and fracture risk. We collected 446 serum samples from postmenopausal women aged ≥45 years old. Based on the BMD measurement, we classified the participants into three groups: osteoporotic, osteopenic, and normal. In an initial discovery stage, we conducted a proteomic approach using two-dimensional differential gel electrophoresis (2D-DIGE). The peptides into the spots of interest were identified through matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF/TOF). Enzyme-linked immunosorbent assay (ELISA) was performed to validate the protein...
Toxicology and Applied Pharmacology, 2019
Hepatocellular carcinoma (HCC) arises after a long period of exposition to etiological factors th... more Hepatocellular carcinoma (HCC) arises after a long period of exposition to etiological factors that might be either independent or collectively contributing. Several rodent models resemble human HCC; however, the major limitation of these models is the lack of chronic injury that reproducibly mimics the molecular alterations as it occurs in humans. Thus, we hypothesized that chronic administration of different DEN treatments identifies the best-fit dose to induce the HCC and/or to determine whether small DEN doses act synergistically with other known hepatotoxins to induce HCC in mice. C57BL/6J male mice were intraperitoneally injected twice a week for 6 weeks with different DEN doses ranging from 2.5 to 40 mg/kg body weight; then, selected doses (2.5, 5 and 20 mg/kg) for 6, 10, 14, and 18 weeks. We demonstrated that DEN at 20 mg/kg promoted reactive oxygen species and 4-hydroxynonenal production, cell proliferation inflammatory infiltrate, and fibrosis, which in turn induced liver cancer by week 18. These parameters were established by evaluating histopathological changes, HCC markers such as glutathione S-transferase placental-1 (Gstp1), Cytokeratin-19 (Ck19) and prostaglandin reductase-1 (Ptgr1); that of Cyp2e1, a DEN metabolizing enzyme; and the expression of the proliferation marker Ki67. While DEN at 2.5 and 5 mg/kg increased Gstp1 and Ck19, DEN at 20 mg/kg decreased them and Cyp2e1 expression and activity. In summary, our results demonstrate that DEN chronically administrated at 20 mg/kg induces the HCC, while DEN at 2.5 and 5 mg/kg could be useful in elucidating its synergistic effect with other hepatotoxic agents in mice.
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, 2017
Polymorphisms (SNPs), Small Integrin-Binding LIgand N-linked Glycoprotein (SIBLING), untranslated... more Polymorphisms (SNPs), Small Integrin-Binding LIgand N-linked Glycoprotein (SIBLING), untranslated region (UTR), amino acid (aa), acute myeloid leukemia (AML), papillary thyroid cancer (PTC), hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC), non-small-cell lung cancer (NSCLC), oral squamous cell carcinoma (OSCC), colorectal carcinoma (CRC), nasopharyngeal carcinoma (NPC), colorectal carcinoma (CRC).
Data in Brief, 2017
This data contains information related to the research article entitled "Osteopontin splice varia... more This data contains information related to the research article entitled "Osteopontin splice variants and polymorphisms in Cancer Progression and Prognosis" [1]. Here, we describe an in silico analysis of transcription factors that could have altered binding to their DNA target sequence as a result of SNPs in the osteopontin gene promoter. We concentrated on SNPs associated with cancer risk and development. The analysis was performed with PROMO v3.0.2 software which incorporates TRANSFACT v6.4 of. We also present a figure depicting the putative transcription factor binding according to genotype.
Cancer Research, 2009
Introduction: Conversion of Protein-SH into disulphides is an early event during the radical-medi... more Introduction: Conversion of Protein-SH into disulphides is an early event during the radical-mediated oxidation of proteins; this has prompted a great interest in the study of changes of these thiol proteins. The aim was to detect the most sensitive thiol proteins to reactive oxygen species generated by metabolism of Diethylnitrosamine (DEN) in the rat liver. DEN has been widely used to induce experimental hepatocellular carcinoma. Methods: Twenty Male Fischer 344 rats were randomized into 4 groups: 3 groups were treated with DEN at 200 mg/kg weight. Each group was sacrificed at 6h (DEN 6), 12h (DEN 12) and 24h (DEN 24) after DEN administration. The last group was used as negative control (NC). To evaluate the formation of disulphide proteins in the liver, we compared the three DEN treated groups against the non treated rats. Total proteins were extracted and labeled with saturation dye according to method Redox-DIGE with some modifications, this method coupled to mass spectrometry ...
Cancer Research, 2009
Background: In recent years the extremely low frequency of electromagnetic fields (ELF-EMF) has b... more Background: In recent years the extremely low frequency of electromagnetic fields (ELF-EMF) has been studied with major interest due their possible effects on the human health. Researches about that are contrasting; whereas some authors associate to ELF-EMF exposure with carcinogenesis, studies in experimental models and human being cancer have shown that the ELF-EMF not increases the risk of several cancer types. Previously, a biophysical model has hypothesized that the action mechanism of ELF-EMF on cells is through forced-vibration of the free ions that exist on all plasma membranes and that can move across of them using transmembrane proteins, disordering the electrochemical balance of the plasma membrane and therefore the whole cell function. In this way, cancer development takes place through rapid proliferation and the continuous increase of altered cells modifying the cellular environment including the flow of ionic charges across the cell membrane; considering that hepatoce...
BMC Cancer, 2010
Background Recently, extremely low frequency electromagnetic fields (ELF-EMF) have been studied w... more Background Recently, extremely low frequency electromagnetic fields (ELF-EMF) have been studied with great interest due to their possible effects on human health. In this study, we evaluated the effect of 4.5 mT - 120 Hz ELF-EMF on the development of preneoplastic lesions in experimental hepatocarcinogenesis. Methods Male Fischer-344 rats were subjected to the modified resistant hepatocyte model and were exposed to 4.5 mT - 120 Hz ELF-EMF. The effects of the ELF-EMF on hepatocarcinogenesis, apoptosis, proliferation and cell cycle progression were evaluated by histochemical, TUNEL assay, caspase 3 levels, immunohistochemical and western blot analyses. Results The application of the ELF-EMF resulted in a decrease of more than 50% of the number and the area of γ-glutamyl transpeptidase-positive preneoplastic lesions (P = 0.01 and P = 0.03, respectively) and glutathione S-transferase placental expression (P = 0.01). The number of TUNEL-positive cells and the cleaved caspase 3 levels wer...
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2013
Conversion of protein-SH groups to disulfides is an early event during protein oxidation, which h... more Conversion of protein-SH groups to disulfides is an early event during protein oxidation, which has prompted great interest in the study of thiol proteins. Chemical carcinogenesis is strongly associated with the formation of reactive oxygen species (ROS). The goal of this study was to detect thiol proteins that are sensitive to ROS generated during diethylnitrosamine (DEN) metabolism in the rat liver. DEN has been widely used to induce experimental hepatocellular carcinoma. We used modified redox-differential gel electrophoresis (redox-DIGE method) and mass spectrometry MALDI-TOF/TOF to identify differential oxidation protein profiles associated with carcinogen exposure. Our analysis revealed a time-dependent increase in the number of oxidized thiol proteins after carcinogen treatment; some of these proteins have antioxidant activity, including thioredoxin, peroxirredoxin 2, peroxiredoxin 6 and glutathione S-transferase alpha-3. According to functional classifications, the identified proteins in our study included chaperones, oxidoreductases, activity isomerases, hydrolases and other protein-binding partners. This study demonstrates that oxidative stress generated by DEN tends to increase gradually through DEN metabolism, causes time-dependent necrosis in the liver and has an oxidative effect on thiol proteins, thereby increasing the number of oxidized thiol proteins. Furthermore, these events occurred during the hepatocarcinogenesis initiation period.
Bioelectromagnetics
Homogeneous extremely low-frequency electromagnetic fields (ELF-EMFs) alter biological phenomena,... more Homogeneous extremely low-frequency electromagnetic fields (ELF-EMFs) alter biological phenomena, including the cell phenotype and proliferation rate. Heterogenous vortex magnetic fields (VMFs), a new approach of exposure to magnetic fields, induce systematic movements on charged biomolecules from target cells; however, the effect of VMFs on living systems remains uncertain. Here, we designed, constructed, and characterized an ELF-VMF-modified Rodin's coil to expose SH-SY5Y cells. Samples were analyzed by performing 2D-differential-gel electrophoresis, identified by MALDI-TOF/TOF, validated by western blotting, and characterized by confocal microscopy. A total of 106 protein spots were differentially expressed; 40 spots were downregulated and 66 were upregulated in the exposed cell proteome, compared to the control cell proteome. The identified spots are associated with cytoskeleton and cell viability proteins, and according to the protein-protein interaction network, a significant interaction among them was found. Our data revealed a decrease in cell survival associated with apoptotic cells without effects on the cell cycle, as well as evident changes in the cytoskeleton. We demonstrated that ELF-VMFs, at a specific frequency and exposure time, alter the cell proteome and structurally affect the target cells. This is the first report showing that VMF application might be a versatile system for testing different hypotheses in living systems, using appropriate exposure parameters.© 2022 Bioelectromagnetics Society.
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Papers by Diana Ivette Aparicio Bautista