Papers by Santina Di Bella
Foods
Contamination of bivalve mollusks with human pathogenic viruses represents a recognized food safe... more Contamination of bivalve mollusks with human pathogenic viruses represents a recognized food safety risk. Thus, monitoring programs for shellfish quality along the entire food chain could help to finally preserve the health of consumers. The aim of the present study was to provide up-to-date data on the prevalence of enteric virus contamination along the shellfish production and distribution chain in Sicily. To this end, 162 batches of mollusks were collected between 2017 and 2019 from harvesting areas, depuration and dispatch centers (n = 63), restaurants (n = 6) and retail stores (n = 93) distributed all over the island. Samples were processed according to ISO 15216 standard method, and the presence of genogroup GI and GII norovirus (NoV), hepatitis A and E viruses (HAV, HEV), rotavirus and adenovirus was investigated by real-time reverse transcription polymerase chain reaction (real-time-RT PCR), nested (RT)-PCR and molecular genotyping. Our findings show that 5.56% of samples we...
West Nile Disease is a zoonotic vector-borne infection involving viral pathogens, human and anima... more West Nile Disease is a zoonotic vector-borne infection involving viral pathogens, human and animal hosts, vectors and habitats with a complex transmission cycle. Cooperation among different disciplines has been promoted by the Italian Public Health Authorities to introduce a robust surveillance system and an integrated West Nile Virus (WNV) Surveillance Plan has been in force in Italy since 2016, in order to establish a medical, veterinary and entomological network. This represents a unique model in Europe. This study aims to present this “One Health” approach applied following the first recorded autochthonous case of West Nile Neuroinvasive Disease (WNND) in Sicily (Southern Italy). Serological and molecular tests were conducted on the blood, liquor and urine of a 38-year-old man with encephalitis and meningitis: WNND was confirmed by serological analysis on liquor and serum. Consequently, a veterinary and entomological surveillance was started. Overall, 160 mosquito catches were c...
Viruses, 2022
Core vaccinations and specific antibody titer evaluations are strongly recommended worldwide by a... more Core vaccinations and specific antibody titer evaluations are strongly recommended worldwide by all the vaccination guidelines. Virus neutralization (VN) is considered the gold standard for measuring antibody titer against canine distemper virus, but it is complex and time consuming, and the use of in-clinics tests would allow to obtain quicker results. The aim of this study was to evaluate the agreement of the commercial in-clinics VacciCheck test compared to VN. A total of 106 canine sera were analyzed using both methods. The best agreement was obtained using a protective threshold of ≥1:32. VacciCheck showed 95.5% sensitivity, 87.2% specificity, and 92.5% accuracy. The Cohen’s kappa coefficient between methods was 0.84 (CI 95% 0.73 to 0.95), revealing an optimal agreement between the two methods (p = 0.0073). The evaluation of discordant results reveal that most samples had less than 1.5 dilution difference, and that usually did not affect the classification as protected or non-p...
Additional file 1. Canine parvovirus type 2 (CPV-2) antigenic variants, amino acid sequence types... more Additional file 1. Canine parvovirus type 2 (CPV-2) antigenic variants, amino acid sequence types (aaSTs), nucleotide sequence types (ntSTs) and nucleotide sequences obtained in this study. Sequences are reported with: year of identification, lab ID number, antigenic variant and GenBank accession number.
Animals, 2021
In Europe, foodborne transmission has been clearly associated to sporadic cases and small cluster... more In Europe, foodborne transmission has been clearly associated to sporadic cases and small clusters of hepatitis E in humans linked to the consumption of contaminated pig liver sausages, raw venison, or undercooked wild boar meat. In Europe, zoonotic HEV-genotype 3 strains are widespread in pig farms but little information is available on the prevalence of HEV positive pigs at slaughterhouse. In the present study, the prevalence of HEV-RNA positive pigs was assessed on 585 animals from 4 abattoirs located across Italy. Twenty-one pigs (3.6%) tested positive for HEV in either feces or liver by real-time RT-PCR. In these 21 pigs, eight diaphragm muscles resulted positive for HEV-RNA. Among animals collected in one abattoir, 4 out of 91 plasma tested positive for HEV-RNA. ELISA tests for the detection of total antibodies against HEV showed a high seroprevalence (76.8%), confirming the frequent exposure of pigs to the virus. The phylogenetic analyses conducted on sequences of both ORF1 a...
Comparative immunology, microbiology and infectious diseases, 2020
Since its emergence in Nigeria, canine parvovirus type 2 (CPV-2) infection has posed problems to ... more Since its emergence in Nigeria, canine parvovirus type 2 (CPV-2) infection has posed problems to dog breeding and requires constant awareness and monitoring. In this study, the status, the assessment of extrinsic risk factors of parvoviral infection in dog kennels in North Central Nigeria, and isolation of the CPV-2 were carried out. Potential risk factors were considered during sampling: age, breed, sex, location, vaccination and health status, using well-structured questionnaires on dog owners with experience of CPV-2 infection. There was high prevalence which depended on age, breed, location, clinical status of the dog while vaccination status of the dogs did not influence the prevalence. CPV-2 vaccination compliance by the breeders and management system of the kennels were also observed as risk factors. Isolation of CPV-2a and -2c strains from Nigeria for further study has been reported. The spread of CPV-2 in Nigeria is increasing, hence needs for continual epidemiological moni...
Veterinaria italiana, 2018
Canine distemper virus (CDV) is the etiologic agent of distemper in dogs. It exhibits an elevated... more Canine distemper virus (CDV) is the etiologic agent of distemper in dogs. It exhibits an elevated potential of crossing species barriers, infecting a wide range of wild and domestic carnivores. Of its encoding genes, hemagglutinin (H) shows high heterogeneity, and it was used to determine the relationship between CDV strains due to its variability and key role in determining cell tropism, host shift, and in eliciting a protective immune response. This study analysed the full-length H gene sequence of Arctic-like CDV strains collected from dogs in Italy during a period in which an increased activity of CDV diffusion was observed. The common amino acid changes and features of Arctic-like CDV strains collected from 2011 to 2016 in Europe were described, providing an updated analysis of the genomic features. A comparison with CDV vaccine strains was carried out to evaluate the increased genomic difference with CDV Arctic-like field strains. This study provides a complete and updated ana...
Animals, 2020
Vector-borne pathogens such as Erlichia canis and Rickettsia conorii are widespread in the Medite... more Vector-borne pathogens such as Erlichia canis and Rickettsia conorii are widespread in the Mediterranean basin. Rhipicephalus sanguineus, is considered the main vector in Mediterranean climatic areas. Seroprevalence in dogs is variable in relation to environmental factors, presence of vectors, and dogs’ management. We investigated the seroprevalence in Sicilian dogs during 2017–2019, considering temporal as well as spatial variations, and different canine population. A total of 11,009 sera were analyzed: 7568 and 3441 sera were tested to detect antibodies to E. canis and to R. conorii, respectively, by immunofluorescence assay. The rainfall average in the sampling sites during the three-year period was also considered. Statistical analyses were performed using chi-square tests for association between two or more variables. We reported a prevalence of 29.6% and 53.6% for E. canis and R. conorii, respectively. Significant temporal variation was found in R. conorii, while significant d...
Animals, 2021
Mesenchymal stem cells (MSCs) are used in therapy in animal models and veterinary medicine, due t... more Mesenchymal stem cells (MSCs) are used in therapy in animal models and veterinary medicine, due to their capacity of inducing tissue regeneration and immunomodulation. Their clinical application requires a ready off-the-shelf amount of viable therapeutics doses. For this purpose, it is useful to cryopreserve MSCs to gain a ready and controlled source of abundant autologous stem cells. We evaluated the effect of 7 years cryopreservation using 10% dimethyl sulfoxide (DMSO) with different fetal bovine serum (FBS) concentrations (from 10 to 90%) on different passages of MSCs isolated from canine adipose tissue (cAD-MSCs). The study aimed to evaluate the most adequate cell passage and FBS percentage for the long-term cryopreservation of cells by maintaining the stemness features. Phenotype morphology, cell viability, osteogenic and adipogenic differentiation potentials, proliferative potential and expression of pluripotency markers were analyzed in thawed cells and compared with fresh on...
Veterinarski arhiv, 2021
Hepatitis E virus (HEV) is considered to be an emerging zoonotic disease, which causes numerous c... more Hepatitis E virus (HEV) is considered to be an emerging zoonotic disease, which causes numerous cases of hepatitis and deaths worldwide each year. Pigs are a host reservoir of HEV, but numerous other wild and domestic species can be infected. The aim of this study was to carry out serological screening of anti-HEV antibodies in cattle in the Sicily region (Southern Italy). Between April and December 2018, 231 serum samples were collected from cows and analysed with enzyme-linked immunosorbent assay (ELISA), for detection of HEV antibodies (IgG). The overall prevalence of anti-HEV antibodies was 36.36% (84/231; 95% CI 30-43). There were statistically significant differences in the HEV seroprevalence in animals of different ages (<11 months: 11.41%; >12 months: 39.30%; >24 months: 54.10%) (P<0.05). No statistically significant differences were detected between genders (31.37% in male and 37.38% in female) (P >0.05). This study shows that further investigation of HEV in ...
Microorganisms, 2020
Borrelia burgdorferi is a bacterial pathogen transmitted by Ixodes ticks and is responsible for L... more Borrelia burgdorferi is a bacterial pathogen transmitted by Ixodes ticks and is responsible for Lyme disease in both humans and dogs. The aim of this work was to evaluate B. burgdorferi diffusion among stray dogs in Palermo (Sicily, Italy) by serological methods in order to study the risk factors associated with the infection. Serum and blood samples of 316 dogs were collected from a shelter in Palermo, and were analyzed for the presence of antibodies against B. burgdorferi by indirect immunofluorescence assay (IFA), and of the ospA gene by real-time PCR, respectively. Seventeen sera (5.4%) were positive for the antibodies via IFA and one blood (0.3%) for ospA via real time PCR. On the basis of serological results, the evaluation of the potential risk factors (sex, age, breed and coat color) was carried out. The multivariate analysis indicated that male sex is a factor significantly associated with B. burgdorferi seropositivity. This study confirms that male dogs have a higher risk ...
Veterinary Medicine and Science, 2021
Abstract Background West Nile Disease (WND) is a zoonotic mosquito‐borne infection involving vira... more Abstract Background West Nile Disease (WND) is a zoonotic mosquito‐borne infection involving viral pathogens, human and animal hosts, vectors and environment. Cooperation among medical, veterinary and entomological fields has been promoted by the Italian Public Health Authorities, and an integrated West Nile Virus (WNV) Surveillance Plan has been in force in Italy since 2016 to prevent the transmission risk of WND to humans through an early detection of viral circulation by animal and entomological surveillance. This managing model is unique in Europe. Objectives This survey aimed at presenting the ‘One Health’ approach applied in 2016 to the first autochthonous human case of West Nile Neuroinvasive Disease (WNND) in Sicily (Southern Italy). Methods Serological (anti‐WNV IgM and IgG ELISA, anti‐WNV neutralizing antibodies) and molecular tests were conducted on blood, liquor and urine of a 38‐year‐old man with encephalitis and meningitis. Overall, 2704 adult culicides from 160 mosquito catches were morphologically identified. Female mosquitoes were analysed in pools for WNV RNA detection. Serological (anti‐WNV IgM and IgG ELISA) and molecular analyses for WNV were carried out in 11 horses, 271 chickens and two dogs sampled in farms around the man's residence. Results and conclusions WNND was confirmed by serological analysis on patient's liquor and serum. Collected mosquito species included Culex pipiens (93.56%, CI95% 92.64%–94.49%), Aedes albopictus (5.25%, CI95% 4.41%–6.09%), Culex hortensis (0.59%, CI95% 0.30%–0.88%), Culiseta longiareolata (0.55%, CI95% 0.27%–0.83%) and Anopheles maculipennis s.l. (0.04%, CI95% –0.04% to 0.11%). Mosquito pools were negative for WNV RNA. Two dogs (100%) and two horses (18.18%, CI95% –4.61 to 40.97%) resulted positive for anti‐WNV specific antibodies. The ‘One Health’ approach allowed to report the first human neuroinvasive WND in Sicily and to confirm the local circulation of WNV in animals of the same area where the clinical case occurred, defining the autochthonous origin of the infection.
Viruses, 2021
Equid and asinine gammaherpesviruses (GHVs; genus Percavirus) are members of the Herpesviridae fa... more Equid and asinine gammaherpesviruses (GHVs; genus Percavirus) are members of the Herpesviridae family. Though GHVs have been reported in horse populations, less studies are available on gammaherpesviral infections in donkeys. This study reports the co-infection with two GHVs in Pantesco breed donkeys, an endangered Italian donkey breed. Samples (n = 124) were collected on a breeding farm in Southern Italy from 40 donkeys, some of which were healthy or presented erosive tongue lesions and/or mild respiratory signs. Samples were analysed by using a set of nested PCRs targeting the DNA polymerase, glycoprotein B, and DNA-packaging protein genes, and sequence and phylogenetic analyses were performed. Twenty-nine donkeys (72.5%) tested positive, and the presence of Equid gammaherpesvirus 7 and asinine herpesvirus 5 was evidenced. In 11 animals, we found evidence for co-infection with viruses from the two species. Virions with herpesvirus-like morphology were observed by electron microsco...
BioMed Research International, 2020
Objective. Biomaterial research for soft tissue augmentation is an increasing topic in aesthetic ... more Objective. Biomaterial research for soft tissue augmentation is an increasing topic in aesthetic medicine. Hyaluronic acid (HA) fillers are widely used for their low invasiveness and easy application to correct aesthetic defects or traumatic injuries. Some complications as acute or chronic inflammation can occur in patients following the injection. Biocompatibility assays are required for medical devices intended for human use, in order to prevent damages or injuries in the host. In this study, nine HA fillers were tested in order to evaluate their cytotoxicity and their effects on L929 cell line, according to the UNI EN ISO 10993 regulation. Methods. Extracts were prepared from nine HA fillers, and MTS viability assay was performed after 24 h, 48 h, and 72 h of exposure of cells to extracts. Cells cultured with HA filler extracts were monitored for up to 72 h, counted, and stained with haematoxylin/eosin in order to evaluate the cell proliferation rate and morphology. Results. None...
Biodiversity Journal, 2019
Betanodavirus infection is widespread in a broad spectrum of fish species worldwide. In Italy, it... more Betanodavirus infection is widespread in a broad spectrum of fish species worldwide. In Italy, it is responsible for outbreaks of Viral Encephalo-Retinopathy (VER) that causes mortality and economic losses in sea fish farming. The infection is also widespread in wildlife, where there are generally no observed clinical manifestations. In this study we report the results obtained from the decennial activity of Istituto Zooprofilattico Sperimentale of Sicily on the research of Betanodavirus infection in wild fish of Mediterranean Sea and in farmed fish. Among the fish species analyzed, Gobius niger (Linnaeus, 1758), Mullus barbatus (Linnaeus, 1758), and Trisopterus minutus capelanus (Lacepède, 1800) were found positive and these could be a reservoir in which the virus can survive for long periods of time. The Betanodavirus isolation from pelagic species such as Pagellus erythrinus (Linnaeus, 1758), Sardina pilchardus (Walbaum, 1792), Lepidopus caudatus (Euphrasen, 1788), Epinephelus marginatus (Lowe, 1834), Epinephelus aeneus (Geoffroy Saint-Hilaire, 1817) resulted interesting because these species could play a more significant epidemiological role, being able to move even at distances.
Biodiversity Journal, 2019
Biodiversity Journal, 2019
BMC Veterinary Research, 2019
Background Canine parvovirus type 2 (CPV-2) is the most important enteric virus infecting canids.... more Background Canine parvovirus type 2 (CPV-2) is the most important enteric virus infecting canids. It is a rapidly evolving virus; after its emergence in the 1970s, new antigenic variants (called CPV-2a, 2b and 2c) emerged and replaced the original antigenic type. The three antigenic variants are globally distributed with different frequencies and levels of genetic variability. This study focused on VP2 gene sequence analysis and the phylodynamics of CPV-2 which were detected in 123 dogs showing clinical signs of gastroenteritis collected in Italy from 1994 to 2017. Results For the most part, the sick dogs were young, and a third of them (32.5%) had been vaccinated. No statistical association was found between the CPV-2 antigenic variants, and sex, age, breed and vaccination status. Sequence analysis showed that all three antigenic types circulated in Italy; the CPV-2a type was the prominent genotype, followed by CPV-2c and CPV-2b, with notable differences regarding regional bases an...
BioMed Research International, 2019
Objective. The aim of this study was to evaluate the cytotoxic potential of a type of endodontic ... more Objective. The aim of this study was to evaluate the cytotoxic potential of a type of endodontic pin on L929 cell line according to the UNI EN ISO 10993/2009 rule. Methods. L929 cells were used for the assays; extracts were prepared from three different-diameter endodontic pins, made of epoxy resin and fiberglass matrix and from Reference Materials (ZDEC, ZDBC, and HDP films). MTS assay was performed after 24 h, 48 h, and 72 h of exposure of L929 cells to pin and Reference Material extracts, 5% phenol solution, and control reagent. Cells cultured with different media containing extracts were monitored for up to 72 h and stained with haematoxylin/eosin. Results. Pins of different diameters had no cytotoxic effects on L929 cells at 24 h, 48 h, and 72 h (all values >70%). Cells cultured in medium containing pin extracts grew without any differences compared to the control cells. Conclusion. The endodontic pins tested showed no cytotoxic effects and did not induce changes in morpholo...
Transboundary and Emerging Diseases, 2019
Canine parvovirus (CPV) is a small (about 25 nm in diameter), nonenveloped DNA virus, which emerg... more Canine parvovirus (CPV) is a small (about 25 nm in diameter), nonenveloped DNA virus, which emerged suddenly in the late 1970s as pathogen of dogs, causing severe and often fatal epizootics of gastroenteritis worldwide (Decaro & Buonavoglia, 2012; Decaro, Desario, et al., 2007). CPV was recently included, together with feline panleukopenia virus (FPLV), mink enteritis virus (MEV) and raccoon parvovirus (RaPV), in the unique species Carnivore protoparvovirus 1, within the Protoparvovirus genus (family Parvoviridae, subfamily Parvovirinae) (Cotmore et al., 2019, 2014). Its genome consists of a 5200-nucleotide (nt) DNA molecule containing two large open
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Papers by Santina Di Bella