Papers by David S. Barkley
Science, 1977
Huntington's disease is a hereditary, chronic, degenerative disease of the brain which is... more Huntington's disease is a hereditary, chronic, degenerative disease of the brain which is transmitted by an autosomal dominant gene. We have discovered that lymphocytes from patients with Huntington's disease respond to the presence of brain tissue from patients with the disease by producing migration inhibition factor, a correlate of the cellular immune response. Lymphocytes from donors without the disease do not respond to the diseased brain tissue, and lymphocytes from patients with Huntington's disease respond only rarely to brain tissue from donors without the disease.
The American Naturalist, 1968
... 925 The American Naturalist May-June, 1968 CYCLIC AMP: A NATURALLY OCCURRING ACRASIN IN THE C... more ... 925 The American Naturalist May-June, 1968 CYCLIC AMP: A NATURALLY OCCURRING ACRASIN IN THE CELLULAR SLIME MOLDS* TM KONIJN,t DS BARKLEY,T YY CHANG, AND JT BONNER Department of Biology, Princeton University, Princeton, New Jersey 08540 ...
Proceedings of the National Academy of Sciences, 1967
The life cycle of the cellular slime mold is marked by a phase of vegetative reproduction and, af... more The life cycle of the cellular slime mold is marked by a phase of vegetative reproduction and, after the exhaustion of the food supply, a phase of morphogenetic activity. The first visible event to occur after the onset of starvation is the aggregation of the myxamoebae into a mound of cells which subsequently differentiates into a migrating slug and ultimately into a fruiting body of varying complexity.
Neurology, 1978
This study confirms the production of migration inhibition factor by Huntington disease lymphocyt... more This study confirms the production of migration inhibition factor by Huntington disease lymphocytes in response to antigen found in Huntington disease but not normal brain tissue. Huntington disease lymphocytes also respond to the presence of multiple sclerosis brain tissue with migration inhibition factor production.
Journal of Cellular Physiology, 1973
Trypsinized cells of newborn mouse cerebellum have been separated by velocity sedimentation at un... more Trypsinized cells of newborn mouse cerebellum have been separated by velocity sedimentation at unit gravity in shallow gradients of Ficoll. The two main technical difficulties were formation of gels around the dissociated cells and clumping of cells before and during the sedimentation procedure. These were solved by adding DNase to the dissociation medium and with holding serum, respectively. Proliferating cells of the external granular layer separated according to size differences in the cell generation cycle. Identification of Purkinje or other early‐forming neurons was made by labeling them with 3H‐thymidine on their birthdays. Many of the fractions contain viable cells capable of aggregating in culture.
Developmental Biology, 1969
Stain Technology, 1971
H2 styrene-glycolmethacrylate is a suitable standard source for autoradiographic model studies. W... more H2 styrene-glycolmethacrylate is a suitable standard source for autoradiographic model studies. When it is used for quantitative autoradiography, the coefficient of variation for grain counts is minimal at a section thickness of 2.5 μ and greater. Variation increases with progressively thinner sections. When sections of the same thickness are mounted on separate slides, there is no significant variation in grain counts at the 95% confidence level when the slides are processed simultaneously.
Annals of Neurology, 1978
Analytical Biochemistry, 1987
Cellular Immunology, 1977
Lymphocytes from patients with Huntington's disease (H.D.) exhibit a cellular immune response whi... more Lymphocytes from patients with Huntington's disease (H.D.) exhibit a cellular immune response which consists of two components: a minor component directed against antigen common to all human brains, and a major component directed against antigen found thus far only in H.D. and multiple sclerosis (M.S.) gray matter. M.S. lymphocytes exhibit a response to antigen common to all human brains, but fail to show any H.D. or M. S. brain-specific response.
Annals of Neurology, 1977
Culture characteristics in a variety of media were determined in 18 skin fibroblast cultures from... more Culture characteristics in a variety of media were determined in 18 skin fibroblast cultures from 8 subjects with Huntington's disease (HD) and 20 cultures from 10 control subjects. Skin fibroblasts from HD subjects consistently and reproducibly demonstrated maximal cell densities that were higher than control values. There was no significant difference in growth rates. The relationship of this phenomenon to the HD gene is unknown, as are its specificity and time of appearance in the course of the illness.
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Papers by David S. Barkley