The objective of this study was to evaluate the influence of the storage environment on quality o... more The objective of this study was to evaluate the influence of the storage environment on quality ofamaranth seed. The seeds were placed in polyethylene bags and stored for ten months in cold andin laboratory conditions. To evaluate the physiological quality of seeds, we determined the water,the one hundred seed weight, germination and vigor. The storage of amaranth in the cold chamberwas more efficient for the conservation of physiological seed quality.O objetivo deste trabalho foi avaliar a Influência do ambiente de armazenamento na qualidadefisiológica de sementes de amaranto. As sementes foram acondicionadas em embalagem depolietileno e armazenadas durante dez meses em câmara fria e em condições ambientais delaboratório. Para avaliação da qualidade fisiológica das sementes, foi determinado o teor deágua, o peso de cem sementes, a germinação e o vigor. O armazenamento de amaranto emcâmara fria foi mais eficiente para conservação da qualidade fisiológica das sementes. Influence of e...
O objetivo deste trabalho foi detectar os constituintes quimicos dos extratos de folhas e cascas ... more O objetivo deste trabalho foi detectar os constituintes quimicos dos extratos de folhas e cascas de sete especies arboreas do Cerrado, isto e, materiais vegetais compostos de folhas e cascas de plantas adultas de Brosimum gaudichauddi , Eugenia dysenterica , Astronium fraxinifolium , Myracrodruon urundeuva , Solanum lycocarpum , Solanum paniculatum e Anacardium humile . Os procedimentos para extracao e identificacao dos constituintes quimicos foram adotados em funcao da classe quimica. Assim, observaram-se resultados positivos para flavonoides, taninos, antraquinonas, alcaloides, catequinas, saponinas, polissacarideos, e acidos orgânicos. A. humile foi a unica planta a apresentar todas as substâncias analisadas. As especies arboreas estudadas apresentaram alto potencial medicinal e farmacologico.
The Syagrus oleracea is adapted for dry regions, has used for food, ornamental palm and developme... more The Syagrus oleracea is adapted for dry regions, has used for food, ornamental palm and development of cosmetic. It occurs in impacted areas of Cerrado. The management of the species is important for traditional communities. Morphological descriptors, biometric pyrenes and spatial distribution of individuals are important for the species distinction, conservation, forest management and implantation of breeding programs. Thus, our objectives were to determine and correlate the main morphological characters of the palm tree, to evaluate the biometric of the pyrenes and the spatial distribution of the species. Neighbourhood Density Function (NDF) evaluated the spatial distribution in georeferenced individuals in four populations (Mirabela, Mato Verde, Rio Pardo de Minas and Novorizonte). The Novorizonte population showed an aggregate pattern in the first distance classes. The other populations had a random pattern. We evaluated 13 morphological descriptors in six populations (Mirabela,...
Flora - Morphology, Distribution, Functional Ecology of Plants, 2015
Fragmentation of tropical forests is one of the major threats to biodiversity and viable natural ... more Fragmentation of tropical forests is one of the major threats to biodiversity and viable natural populations. Brazilian seasonally dry tropical forests (SDTF) are endangered because of human occupation, conversion of lands to agriculture and high deforestation rates in these fertile soils. Enterolobium contortisiliquum has been removed from SDTF natural areas due to the advance of cattle grazing and agriculture in Brazilian SDTFs. To aid conservation efforts of this species we used molecular markers to study the genetic diversity and population structure of E. contortisiliquum in Brazilian SDTF remnants. A total of 263 individuals in 13 forest patches were analyzed with 103 ISSR fragments. In spite of being found scattered among the SDTF patches, E. contortisiliquum populations exhibited high genetic diversity (mean Shannon's index of diversity = 0.384; mean genetic diversity = 0.280) and genetic divergence between populations was detected (˚S T = 0.155, p < 0.0001). UPGMA dendrogram, principal coordinate analysis and Bayesian analysis showed that E. contortisiliquum populations were clustered into three groups that were not related to geographic distance (r = 0.119, p = 0.197). However, these populations are spatially structured into groups distributed in the West, Central and East of the study area. This genetic structure may be related with landscape and both the Espinhaç o Range and São Francisco River may act as a partial geographic barrier limiting the gene flow. Because the highest rates of genetic diversity were found outside of conservation units, we propose the creation of protected areas in different geographic regions that include E. contortisiliquum populations from different genetic clusters.
The bark of the underground stem of Tontelea micrantha (Mart. ex. Schult.) A. C. Sm., a native Br... more The bark of the underground stem of Tontelea micrantha (Mart. ex. Schult.) A. C. Sm., a native Brazilian Cerrado species, is used in folk medicine for treating kidney ailments. The structures of the underground and the aerial stems were examined and their barks were analyzed for the presence of secondary metabolites. Bark fragments were processed according to conventional techniques in plant anatomy and their chemical compositions examined using histochemical and phytochemical tests, thin layer chromatography, and high-efficiency liquid chromatography. The underground stem is a sobol with unusual cambial activity. Laticifers that secrete terpenoids were present in the cortex and phloem of both organs and can contribute to the identification of the species in field. Druses were present in both barks, but mono-crystals were only observed in the sobol. Tannins, flavonoids, alkaloids, and terpenoids occurred in both types of bark, but carotenoids were only detected in the sobol. The sim...
Os teores de ácidos graxos da fração óleo das sementes de soja não têm sido considerados na avali... more Os teores de ácidos graxos da fração óleo das sementes de soja não têm sido considerados na avaliação da qualidade das sementes em trabalhos de pesquisa recentes. O objetivo deste trabalho foi determinar o efeito da ausência de lipoxigenases e baixo teor de ácido linolênico na qualidade de sementes de soja colhidas em diferentes épocas após o estádio R8. Quatro "bulks" de sementes de soja foram formados, com as características: (a) presença de lipoxigenases e teor normal de ácido linolênico, (b) presença de lipoxigenases e baixo teor de ácido linolênico, (c) ausência de lipoxigenases e teor normal de ácido linolênico e (d) ausência de lipoxigenases e baixo teor de ácido linolênico. Sementes de cada "bulk" da geração F5 foram colhidas nos estádios R8, R8+10, R8+20 e R8+30 dias. A qualidade fisiológica das sementes foi avaliada através dos testes de germinação, envelhecimento acelerado e índice de velocidade de emergência. Foi determinado também os teores de ácido ...
Espécies florestais que possuem sementes duras e impermeáveis à água freqüentemente apresentam pr... more Espécies florestais que possuem sementes duras e impermeáveis à água freqüentemente apresentam problemas para germinar. Este trabalho teve como objetivo selecionar tratamentos pré-germinativos em laboratório que permitam abreviar, aumentar e uniformizar a germinação das sementes de Dimorphandra mollis, além de avaliar essa germinação em diferentes épocas e locais de coleta. Foram coletadas sementes de D. mollis em quatro municípios do norte de Minas Gerais: Montes Claros, Lontra, Mirabela e Jequitaí, em duas datas distintas: agosto e setembro de 2004. As sementes foram submetidas a cinco tratamentos de escarificação: lixamento, imersão em ácido sulfúrico por 10 e por 20 min, imersão em água quente a 70 ºC e controle (sementes intactas). Nos testes de germinação, um delineamento experimental casualizado foi utilizado, com 10 repetições de 10 sementes de cada tratamento, localidade e data de coleta. Os efeitos dos diferentes tratamentos na germinação das sementes foram avaliados atrav...
Fava d'anta (Dimorphandra mollis Benth) é uma planta nativa do Cerrado brasileiro utilizada n... more Fava d'anta (Dimorphandra mollis Benth) é uma planta nativa do Cerrado brasileiro utilizada na extração da rutina, quercetina e ramnose, produtos usados nas indústrias farmacêuticas e de cosméticos. O norte do Estado de Minas Gerais produz cerca de 23% da rutina nacional. A obtenção dessa espécie tem sido de forma predatória pelos extrativistas, e estudos da variabilidade genética dessas populações podem fornecer subsídios para estratégias de conservação e manutenção da espécie. O objetivo deste trabalho foi analisar a diversidade genética dessa planta por meio da técnica de RAPD. Para isso, foram coletadas folhas jovens de fava d'anta em sete localidades diferentes (Januária, Patis, Mirabela, Lontra, CAA, Jequitaí e Morro Alto) da região Norte de Minas Gerais. Nessa análise, testaram-se 43 primers. A análise de variância molecular (AMOVA) indicou que 10,3% e 89,7% da variação genética foi distribuída entre e dentro das populações, respectivamente. A diversidade genética de ...
The objective of this study was to investigate the antibacterial activity of crude and semi-pure ... more The objective of this study was to investigate the antibacterial activity of crude and semi-pure extracts obtained from Myracrodruon urundeuva (Aroeira do sertão) against clinically important ATCC bacterial strains by preparative thin layer chromatography (TLC). The results indicate potential activity as assessed by minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) analysis. The crude and semi-pure extracts, obtained from the leaves and bark of M. urundeuva (Fr. All.), have a broad antibacterial spectrum and are capable of inhibiting the growth of different Grampositive and Gram-negative bacterial strains. The phytochemical analysis of the leaves revealed the presence of hydrolysable tannins, flavonoids, saponins, terpenes and steroids. However, extracts from the Aroeira tree leaves showed larger inhibition zones for the species of Gram-positive bacteria than for Gram-negative species. It is possible that the active compounds present in these extracts act by inhibiting the synthesis of peptidoglycan in the cell wall, particularly in Gram-positive bacteria. The proven antibiotic action of crude and semi-pure extract from Aroeira leaves can be explored to facilitate their application as pharmaceutical adjuvants in antibiotic preparations.
This study is part of a thesis presented by P.A. Moreira to the Universidade Federal de Minas Ger... more This study is part of a thesis presented by P.A. Moreira to the Universidade Federal de Minas Gerais in partial fulfillment of the requirements for the Doctoral degree.
the DNA quantity was also high, with an A 260 /A 280 ratio above 1.8, which is within the optimal... more the DNA quantity was also high, with an A 260 /A 280 ratio above 1.8, which is within the optimal sample range. In contrast, DNA isolation from old leaves was not successful. When the DNA was extracted from old leaves, the DNA was brownish, indicating contamination by phenolic compounds. These metabolites oxidize the DNA irreversibly, which hinders amplification of DNA by PCR by inhibiting the action of enzymes such as Taq polymerase. PCR performed with DNA from young leaves of D. mollis was successful and produced strong bands for RAPD markers.
However, the combined probability of paternity exclusion was high, and the combined probability o... more However, the combined probability of paternity exclusion was high, and the combined probability of genetic identity was low. None of the pairs of loci were in linkage disequilibrium. The informative power of these loci demonstrates that they are suitable for studies of diversity and genetic structure of natural populations of A. humile. In addition, the loci are suitable for estimating gene flow between populations, assessing species crossing preferences, and performing interspecific comparisons.
This work aimed to identify the quantitative trait loci (QTL) associated with photosynthesis and ... more This work aimed to identify the quantitative trait loci (QTL) associated with photosynthesis and growth and productivity traits of soybean and to study possible associations between these traits by the analysis of coincidence of QTL in linkage groups (LGs). Thus, populations of recombinant inbred lines (RILs) of the F7 and F8 generations derived from the cross between the varieties BARC-8 and Garimpo were used. The traits evaluated were net assimilation rate of CO2 under saturating light (Asat), potential photosynthesis rate (Pmax), leaf area (A), specific leaf area (SLA), specific leaf nitrogen (N); root (W R), nodule (W N), stem (W ST), leaf (W L), pod (W P) and plant dry mass (W T); nodule (nN), seed (n s), and pod number (nP); seed fresh mass per plant (W S), one-hundred seed fresh mass (W HS) and seed protein percentage (P%). It was possible to identify the following QTL associated with the following soybean traits: SLA, Asat, N, W R, W ST, W L, W T, W P, W HS, n s and nP, indi...
Introdução O desaparecimento de espécies vegetais, de relevante importância sócio-econômica, caus... more Introdução O desaparecimento de espécies vegetais, de relevante importância sócio-econômica, causada pela ação antrópica exige o estabelecimento de tecnologias eficazes voltadas à conservação de genes para as futuras gerações (MEDEIROS et al., 1997). Por ...
Caryocar brasiliense is a widely distributed but endangered Brazilian Cerrado tree species. We st... more Caryocar brasiliense is a widely distributed but endangered Brazilian Cerrado tree species. We studied patterns of spatial genetic structure and populational differentiation from four natural populations in the North of the State of Minas Gerais, Brazil. Genetic diversity from 240 individuals using allozyme loci was analyzed and inferred by heterozygosity index, Nason's kinship coefficient and assessment of historical population bottlenecks. We did not find evidence of inbreeding within fragments, but from the fitting tests into the infinite allele model of mutation no population was shown to be in equilibrium, indicating recent bottlenecks. Only one population (Japonvar) showed positive coancestry value indicating the tendency of increased structure among trees that are spatially closer to each other. Although the average genetic differentiation in each population pair was generally low (D G ¼ 0.036), Mantel tests showed tendency for distance dependence of genetic structure (r m ¼ 0.905, P ¼ 0.084). Fragmentation may cause genetic isolation by next generations, increasing differentiation among populations. Thus, considering the practically irreversible fragmentation of populations around agricultural areas at Cerrado Biome, landscape management strategies for C. brasiliense should be considered to protect the still existent populations.
Revista Brasileira de Gestão Ambiental e Sustentabilidade, 2020
Espécies de palmeiras (Arecaceae) são fontes de matérias-primas renováveis para atender às demand... more Espécies de palmeiras (Arecaceae) são fontes de matérias-primas renováveis para atender às demandas das indústrias de alimentos, das farmacêuticas, de biocombustíveis, de cosméticos e outras. O presente trabalho teve como objetivo identificar a produção de conhecimento científico e tecnológico com o uso das palmeiras Mauritia flexuosa Lin. Fil. (buriti), Acrocomia aculeata (Jacq.) Lodd. ex Mart. (macaúba) e Syagrus oleracea (Mart.) Becc. (guariroba), que são utilizadas para desenvolvimento de produtos em diferentes áreas. Foram realizadas pesquisas exploratórias no banco de dados da Web of Science e a proteção de tecnologias em bancos de dados de patentes do Instituto Nacional de Propriedade Industrial (INPI), Escritório Europeu de Patentes (ESPACENET) e Escritório Latino--Americano de Patentes (LATIPAT). Os resultados permitiram identificar 223 publicações de artigos com buriti e 33 depósitos de patentes, para a macaúba, foram identificadas 192 publicações e 20 depósitos de patente...
The species Syagrus oleracea is a native palm tree, present in highly impacted environments of th... more The species Syagrus oleracea is a native palm tree, present in highly impacted environments of the Brazilian semiarid. This species is exploited for use in the manufacture of folk crafts and for human and animal feeding. The objective of this study was to characterize the diversity and genetic structure of individuals from different populations, located in anthropic regions of the Brazilian semiarid region, in the state of Minas Gerais. This information is important for species management and conservation strategies. Thus, we used ISSR molecular markers in 157 individuals in seven populations of S. oleracea. Looking at the results, one can observe that amplifications of 109 locos occurred and that an average of 18, 17 alleles were found on each primer, values that demonstrated that the ISSR technique used was highly efficient. The genetic diversity indexes were observed, showing expected heterozygosity (He) and Shannon index (I) of 0.260 and 0.383, respectively. The number of allele...
The objective of this study was to evaluate the influence of the storage environment on quality o... more The objective of this study was to evaluate the influence of the storage environment on quality ofamaranth seed. The seeds were placed in polyethylene bags and stored for ten months in cold andin laboratory conditions. To evaluate the physiological quality of seeds, we determined the water,the one hundred seed weight, germination and vigor. The storage of amaranth in the cold chamberwas more efficient for the conservation of physiological seed quality.O objetivo deste trabalho foi avaliar a Influência do ambiente de armazenamento na qualidadefisiológica de sementes de amaranto. As sementes foram acondicionadas em embalagem depolietileno e armazenadas durante dez meses em câmara fria e em condições ambientais delaboratório. Para avaliação da qualidade fisiológica das sementes, foi determinado o teor deágua, o peso de cem sementes, a germinação e o vigor. O armazenamento de amaranto emcâmara fria foi mais eficiente para conservação da qualidade fisiológica das sementes. Influence of e...
O objetivo deste trabalho foi detectar os constituintes quimicos dos extratos de folhas e cascas ... more O objetivo deste trabalho foi detectar os constituintes quimicos dos extratos de folhas e cascas de sete especies arboreas do Cerrado, isto e, materiais vegetais compostos de folhas e cascas de plantas adultas de Brosimum gaudichauddi , Eugenia dysenterica , Astronium fraxinifolium , Myracrodruon urundeuva , Solanum lycocarpum , Solanum paniculatum e Anacardium humile . Os procedimentos para extracao e identificacao dos constituintes quimicos foram adotados em funcao da classe quimica. Assim, observaram-se resultados positivos para flavonoides, taninos, antraquinonas, alcaloides, catequinas, saponinas, polissacarideos, e acidos orgânicos. A. humile foi a unica planta a apresentar todas as substâncias analisadas. As especies arboreas estudadas apresentaram alto potencial medicinal e farmacologico.
The Syagrus oleracea is adapted for dry regions, has used for food, ornamental palm and developme... more The Syagrus oleracea is adapted for dry regions, has used for food, ornamental palm and development of cosmetic. It occurs in impacted areas of Cerrado. The management of the species is important for traditional communities. Morphological descriptors, biometric pyrenes and spatial distribution of individuals are important for the species distinction, conservation, forest management and implantation of breeding programs. Thus, our objectives were to determine and correlate the main morphological characters of the palm tree, to evaluate the biometric of the pyrenes and the spatial distribution of the species. Neighbourhood Density Function (NDF) evaluated the spatial distribution in georeferenced individuals in four populations (Mirabela, Mato Verde, Rio Pardo de Minas and Novorizonte). The Novorizonte population showed an aggregate pattern in the first distance classes. The other populations had a random pattern. We evaluated 13 morphological descriptors in six populations (Mirabela,...
Flora - Morphology, Distribution, Functional Ecology of Plants, 2015
Fragmentation of tropical forests is one of the major threats to biodiversity and viable natural ... more Fragmentation of tropical forests is one of the major threats to biodiversity and viable natural populations. Brazilian seasonally dry tropical forests (SDTF) are endangered because of human occupation, conversion of lands to agriculture and high deforestation rates in these fertile soils. Enterolobium contortisiliquum has been removed from SDTF natural areas due to the advance of cattle grazing and agriculture in Brazilian SDTFs. To aid conservation efforts of this species we used molecular markers to study the genetic diversity and population structure of E. contortisiliquum in Brazilian SDTF remnants. A total of 263 individuals in 13 forest patches were analyzed with 103 ISSR fragments. In spite of being found scattered among the SDTF patches, E. contortisiliquum populations exhibited high genetic diversity (mean Shannon's index of diversity = 0.384; mean genetic diversity = 0.280) and genetic divergence between populations was detected (˚S T = 0.155, p < 0.0001). UPGMA dendrogram, principal coordinate analysis and Bayesian analysis showed that E. contortisiliquum populations were clustered into three groups that were not related to geographic distance (r = 0.119, p = 0.197). However, these populations are spatially structured into groups distributed in the West, Central and East of the study area. This genetic structure may be related with landscape and both the Espinhaç o Range and São Francisco River may act as a partial geographic barrier limiting the gene flow. Because the highest rates of genetic diversity were found outside of conservation units, we propose the creation of protected areas in different geographic regions that include E. contortisiliquum populations from different genetic clusters.
The bark of the underground stem of Tontelea micrantha (Mart. ex. Schult.) A. C. Sm., a native Br... more The bark of the underground stem of Tontelea micrantha (Mart. ex. Schult.) A. C. Sm., a native Brazilian Cerrado species, is used in folk medicine for treating kidney ailments. The structures of the underground and the aerial stems were examined and their barks were analyzed for the presence of secondary metabolites. Bark fragments were processed according to conventional techniques in plant anatomy and their chemical compositions examined using histochemical and phytochemical tests, thin layer chromatography, and high-efficiency liquid chromatography. The underground stem is a sobol with unusual cambial activity. Laticifers that secrete terpenoids were present in the cortex and phloem of both organs and can contribute to the identification of the species in field. Druses were present in both barks, but mono-crystals were only observed in the sobol. Tannins, flavonoids, alkaloids, and terpenoids occurred in both types of bark, but carotenoids were only detected in the sobol. The sim...
Os teores de ácidos graxos da fração óleo das sementes de soja não têm sido considerados na avali... more Os teores de ácidos graxos da fração óleo das sementes de soja não têm sido considerados na avaliação da qualidade das sementes em trabalhos de pesquisa recentes. O objetivo deste trabalho foi determinar o efeito da ausência de lipoxigenases e baixo teor de ácido linolênico na qualidade de sementes de soja colhidas em diferentes épocas após o estádio R8. Quatro "bulks" de sementes de soja foram formados, com as características: (a) presença de lipoxigenases e teor normal de ácido linolênico, (b) presença de lipoxigenases e baixo teor de ácido linolênico, (c) ausência de lipoxigenases e teor normal de ácido linolênico e (d) ausência de lipoxigenases e baixo teor de ácido linolênico. Sementes de cada "bulk" da geração F5 foram colhidas nos estádios R8, R8+10, R8+20 e R8+30 dias. A qualidade fisiológica das sementes foi avaliada através dos testes de germinação, envelhecimento acelerado e índice de velocidade de emergência. Foi determinado também os teores de ácido ...
Espécies florestais que possuem sementes duras e impermeáveis à água freqüentemente apresentam pr... more Espécies florestais que possuem sementes duras e impermeáveis à água freqüentemente apresentam problemas para germinar. Este trabalho teve como objetivo selecionar tratamentos pré-germinativos em laboratório que permitam abreviar, aumentar e uniformizar a germinação das sementes de Dimorphandra mollis, além de avaliar essa germinação em diferentes épocas e locais de coleta. Foram coletadas sementes de D. mollis em quatro municípios do norte de Minas Gerais: Montes Claros, Lontra, Mirabela e Jequitaí, em duas datas distintas: agosto e setembro de 2004. As sementes foram submetidas a cinco tratamentos de escarificação: lixamento, imersão em ácido sulfúrico por 10 e por 20 min, imersão em água quente a 70 ºC e controle (sementes intactas). Nos testes de germinação, um delineamento experimental casualizado foi utilizado, com 10 repetições de 10 sementes de cada tratamento, localidade e data de coleta. Os efeitos dos diferentes tratamentos na germinação das sementes foram avaliados atrav...
Fava d'anta (Dimorphandra mollis Benth) é uma planta nativa do Cerrado brasileiro utilizada n... more Fava d'anta (Dimorphandra mollis Benth) é uma planta nativa do Cerrado brasileiro utilizada na extração da rutina, quercetina e ramnose, produtos usados nas indústrias farmacêuticas e de cosméticos. O norte do Estado de Minas Gerais produz cerca de 23% da rutina nacional. A obtenção dessa espécie tem sido de forma predatória pelos extrativistas, e estudos da variabilidade genética dessas populações podem fornecer subsídios para estratégias de conservação e manutenção da espécie. O objetivo deste trabalho foi analisar a diversidade genética dessa planta por meio da técnica de RAPD. Para isso, foram coletadas folhas jovens de fava d'anta em sete localidades diferentes (Januária, Patis, Mirabela, Lontra, CAA, Jequitaí e Morro Alto) da região Norte de Minas Gerais. Nessa análise, testaram-se 43 primers. A análise de variância molecular (AMOVA) indicou que 10,3% e 89,7% da variação genética foi distribuída entre e dentro das populações, respectivamente. A diversidade genética de ...
The objective of this study was to investigate the antibacterial activity of crude and semi-pure ... more The objective of this study was to investigate the antibacterial activity of crude and semi-pure extracts obtained from Myracrodruon urundeuva (Aroeira do sertão) against clinically important ATCC bacterial strains by preparative thin layer chromatography (TLC). The results indicate potential activity as assessed by minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) analysis. The crude and semi-pure extracts, obtained from the leaves and bark of M. urundeuva (Fr. All.), have a broad antibacterial spectrum and are capable of inhibiting the growth of different Grampositive and Gram-negative bacterial strains. The phytochemical analysis of the leaves revealed the presence of hydrolysable tannins, flavonoids, saponins, terpenes and steroids. However, extracts from the Aroeira tree leaves showed larger inhibition zones for the species of Gram-positive bacteria than for Gram-negative species. It is possible that the active compounds present in these extracts act by inhibiting the synthesis of peptidoglycan in the cell wall, particularly in Gram-positive bacteria. The proven antibiotic action of crude and semi-pure extract from Aroeira leaves can be explored to facilitate their application as pharmaceutical adjuvants in antibiotic preparations.
This study is part of a thesis presented by P.A. Moreira to the Universidade Federal de Minas Ger... more This study is part of a thesis presented by P.A. Moreira to the Universidade Federal de Minas Gerais in partial fulfillment of the requirements for the Doctoral degree.
the DNA quantity was also high, with an A 260 /A 280 ratio above 1.8, which is within the optimal... more the DNA quantity was also high, with an A 260 /A 280 ratio above 1.8, which is within the optimal sample range. In contrast, DNA isolation from old leaves was not successful. When the DNA was extracted from old leaves, the DNA was brownish, indicating contamination by phenolic compounds. These metabolites oxidize the DNA irreversibly, which hinders amplification of DNA by PCR by inhibiting the action of enzymes such as Taq polymerase. PCR performed with DNA from young leaves of D. mollis was successful and produced strong bands for RAPD markers.
However, the combined probability of paternity exclusion was high, and the combined probability o... more However, the combined probability of paternity exclusion was high, and the combined probability of genetic identity was low. None of the pairs of loci were in linkage disequilibrium. The informative power of these loci demonstrates that they are suitable for studies of diversity and genetic structure of natural populations of A. humile. In addition, the loci are suitable for estimating gene flow between populations, assessing species crossing preferences, and performing interspecific comparisons.
This work aimed to identify the quantitative trait loci (QTL) associated with photosynthesis and ... more This work aimed to identify the quantitative trait loci (QTL) associated with photosynthesis and growth and productivity traits of soybean and to study possible associations between these traits by the analysis of coincidence of QTL in linkage groups (LGs). Thus, populations of recombinant inbred lines (RILs) of the F7 and F8 generations derived from the cross between the varieties BARC-8 and Garimpo were used. The traits evaluated were net assimilation rate of CO2 under saturating light (Asat), potential photosynthesis rate (Pmax), leaf area (A), specific leaf area (SLA), specific leaf nitrogen (N); root (W R), nodule (W N), stem (W ST), leaf (W L), pod (W P) and plant dry mass (W T); nodule (nN), seed (n s), and pod number (nP); seed fresh mass per plant (W S), one-hundred seed fresh mass (W HS) and seed protein percentage (P%). It was possible to identify the following QTL associated with the following soybean traits: SLA, Asat, N, W R, W ST, W L, W T, W P, W HS, n s and nP, indi...
Introdução O desaparecimento de espécies vegetais, de relevante importância sócio-econômica, caus... more Introdução O desaparecimento de espécies vegetais, de relevante importância sócio-econômica, causada pela ação antrópica exige o estabelecimento de tecnologias eficazes voltadas à conservação de genes para as futuras gerações (MEDEIROS et al., 1997). Por ...
Caryocar brasiliense is a widely distributed but endangered Brazilian Cerrado tree species. We st... more Caryocar brasiliense is a widely distributed but endangered Brazilian Cerrado tree species. We studied patterns of spatial genetic structure and populational differentiation from four natural populations in the North of the State of Minas Gerais, Brazil. Genetic diversity from 240 individuals using allozyme loci was analyzed and inferred by heterozygosity index, Nason's kinship coefficient and assessment of historical population bottlenecks. We did not find evidence of inbreeding within fragments, but from the fitting tests into the infinite allele model of mutation no population was shown to be in equilibrium, indicating recent bottlenecks. Only one population (Japonvar) showed positive coancestry value indicating the tendency of increased structure among trees that are spatially closer to each other. Although the average genetic differentiation in each population pair was generally low (D G ¼ 0.036), Mantel tests showed tendency for distance dependence of genetic structure (r m ¼ 0.905, P ¼ 0.084). Fragmentation may cause genetic isolation by next generations, increasing differentiation among populations. Thus, considering the practically irreversible fragmentation of populations around agricultural areas at Cerrado Biome, landscape management strategies for C. brasiliense should be considered to protect the still existent populations.
Revista Brasileira de Gestão Ambiental e Sustentabilidade, 2020
Espécies de palmeiras (Arecaceae) são fontes de matérias-primas renováveis para atender às demand... more Espécies de palmeiras (Arecaceae) são fontes de matérias-primas renováveis para atender às demandas das indústrias de alimentos, das farmacêuticas, de biocombustíveis, de cosméticos e outras. O presente trabalho teve como objetivo identificar a produção de conhecimento científico e tecnológico com o uso das palmeiras Mauritia flexuosa Lin. Fil. (buriti), Acrocomia aculeata (Jacq.) Lodd. ex Mart. (macaúba) e Syagrus oleracea (Mart.) Becc. (guariroba), que são utilizadas para desenvolvimento de produtos em diferentes áreas. Foram realizadas pesquisas exploratórias no banco de dados da Web of Science e a proteção de tecnologias em bancos de dados de patentes do Instituto Nacional de Propriedade Industrial (INPI), Escritório Europeu de Patentes (ESPACENET) e Escritório Latino--Americano de Patentes (LATIPAT). Os resultados permitiram identificar 223 publicações de artigos com buriti e 33 depósitos de patentes, para a macaúba, foram identificadas 192 publicações e 20 depósitos de patente...
The species Syagrus oleracea is a native palm tree, present in highly impacted environments of th... more The species Syagrus oleracea is a native palm tree, present in highly impacted environments of the Brazilian semiarid. This species is exploited for use in the manufacture of folk crafts and for human and animal feeding. The objective of this study was to characterize the diversity and genetic structure of individuals from different populations, located in anthropic regions of the Brazilian semiarid region, in the state of Minas Gerais. This information is important for species management and conservation strategies. Thus, we used ISSR molecular markers in 157 individuals in seven populations of S. oleracea. Looking at the results, one can observe that amplifications of 109 locos occurred and that an average of 18, 17 alleles were found on each primer, values that demonstrated that the ISSR technique used was highly efficient. The genetic diversity indexes were observed, showing expected heterozygosity (He) and Shannon index (I) of 0.260 and 0.383, respectively. The number of allele...
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Papers by Dario Oliveira