The aim of this study was to investigate the mite fauna present in 33 living room and 22 bedroom ... more The aim of this study was to investigate the mite fauna present in 33 living room and 22 bedroom curtain dust samples from 41 different homes in the southern Brazilian city of Campinas, SP. A total of 148 mite bodies were found. Of these, 83 were found in living-room curtain samples (56.1% of total) and 65 were in bedroom curtain dust samples (43.9%). The most frequently observed mite suborders were: Acaridida (n = 79; 53.4%), Actinedida (n = 53; 35.8%), Oribatida (n = 14; 9.5%), and Gamasida (n = 2; 1.3%). The most frequent families were Pyroglyphidae (n = 61; 41.2%), Eriophyidae (n = 25; 16.9%), Tarsonemidae (n = 15; 10.1%), and Glycyphagidae (n = 13; 8.8%). No statistical difference was observed between the number of mites found in the samples from living room and bedroom curtains.O intuito deste estudo foi avaliar as espécies acarinas encontradas em amostras de poeira de cortinas presentes em 33 salas e 22 quartos de um total de 41 domicílios na cidade de Campinas, SP. Foram enc...
International journal of clinical pharmacology and therapeutics, 2004
To compare the bioavailability of a fluconazole 150 mg capsule formulation from Laboratório Teuto... more To compare the bioavailability of a fluconazole 150 mg capsule formulation from Laboratório Teuto Brasileiro Ltd., Brazil (test formulation), and Zoltec 150 mg capsule from Laboratórios Pfizer Ltd., Brazil (reference formulation), in 24 volunteers of both sexes. The study was conducted open with randomized 2-period crossover design and a 2-week washout period. Plasma samples were obtained over a 168-hour interval. Fluconazole concentrations were analyzed by combined reversed-phase liquid chromatography and tandem mass spectrometry (LC/MS/MS) with positive ion electrospray ionization using selected ion monitoring method. From the fluconazole plasma concentration vs. time curves the following pharmacokinetic parameters were obtained: AUC(last), AUC(0-inf) and C(max). Geometric mean of fluconazole/Zoltec 150 mg individual percent ratio was 102.6% for AUC(last), 102.2% for AUC(0-inf) and 109.4% for C(max). The 90% confidence intervals were 97.3-108.2%, 97.0-107.8%, and 103.1-116.0%, res...
The aim of this study was to investigate the mite fauna present in 33 living room and 22 bedroom ... more The aim of this study was to investigate the mite fauna present in 33 living room and 22 bedroom curtain dust samples from 41 different homes in the southern Brazilian city of Campinas, SP. A total of 148 mite bodies were found. Of these, 83 were found in living-room curtain samples (56.1% of total) and 65 were in bedroom curtain dust samples (43.9%). The most frequently observed mite suborders were: Acaridida (n = 79; 53.4%), Actinedida (n = 53; 35.8%), Oribatida (n = 14; 9.5%), and Gamasida (n = 2; 1.3%). The most frequent families were Pyroglyphidae (n = 61; 41.2%), Eriophyidae (n = 25; 16.9%), Tarsonemidae (n = 15; 10.1%), and Glycyphagidae (n = 13; 8.8%). No statistical difference was observed between the number of mites found in the samples from living room and bedroom curtains.
A method based on liquid chromatography with positive ion electrospray ionization and tandem mass... more A method based on liquid chromatography with positive ion electrospray ionization and tandem mass spectrometry is described for the determination of terbinafine in human plasma using naftifine as internal standard. The method has a chromatographic run time of 5 minutes and was linear in the range 1.0 to 2000 ng/mL. The limit of quantification was 1.0 ng/mL; the intraday precision was 3.6%, 3.8%, 3.5%, and 4.1%; and the intraday accuracy was -2.7%, 7.7%, 4.8%, and -2.7% for 5.0, 80.0, 250.0, and 1500.0 ng/mL, respectively. The interday precision was 4.9%, 1.7%, 2.4%, and 4.6% and the interday accuracy was 0.3%, 5.8%, 6.5%, and -1.4% for the same concentrations. This method was used in a bioequivalence study of two tablet formulations of terbinafine. Twenty-four healthy volunteers (both sexes) received a single oral dose of terbinafine (250 mg) in an open, randomized, two-period crossover study. The 90% CI of geometric mean ratios between Terbinafina (Medley S/A Indústria Farmacêutica, Campinas, Brazil) and Lamisil (Novartis Biociências S/A, São Paulo, Brazil) were 90.5% to 110.0% for C max, 92.2% to 108.1% for AUC last, and 91.3% to 107.5% for AUC 0-inf. Because the 90% CI for the above-mentioned parameters were included in the 80% to 125% interval proposed by the US FDA, the two formulations were considered bioequivalent in terms of rate and extent of absorption.
In Brazil, a herbal medicinal extract named Catuama containing a mixture of Paullinia cupana (gua... more In Brazil, a herbal medicinal extract named Catuama containing a mixture of Paullinia cupana (guarana; Sapindaceae), Trichilia catigua (catuaba; Meliaceae), Ptychopetalum olacoides (muirapuama; Olacaceae) and Zingiber officinale (ginger; Zingiberaceae) is used as a body stimulant, energetic, tonic and aphrodisiac. The present study investigated the chronic administration of 25 mL Catuama twice a day during 28 days for any toxic effect on healthy human volunteers of both sexes. No severe adverse reactions or haematological and biochemical changes were reported.
The aim of this study was to investigate the mite fauna in mattresses dust samples from cribs or ... more The aim of this study was to investigate the mite fauna in mattresses dust samples from cribs or beds in the south Brazilian city of Londrina, State of Paraná. A total of 133 dust samples from upper and lower mattress surfaces, and bed frames were aspirated once from 38 dwellings (18 cribs and 21 beds), and one day nursery (six cribs). A total of 758 mite bodies were counted in slides: 233 (30.7%) from cribs and 525 (69.3%) from beds (p < 0.001). House dust mites – mainly Dermatophagoides pteronyssinus, represented 72% and 84% of total mite count in crib and bed dust samples, respectively. The mean HDM body concentration in crib or bed slides were, respectively, 289.9 ± 136.7 and 875.0 ± 183.6 mites/g. Statistical analysis showed a significantly higher mite bodies count on lower mattress surface compared with upper surface in bed samples only (p = 0.025). Data herein show that cribs like mattress have sufficient mite bodies to cause sensitization to humans. The use of mattress co...
House dust mites have been reported to be the most important allergen in human dwellings. Several... more House dust mites have been reported to be the most important allergen in human dwellings. Several articles had already shown the presence of different mite species at homes in Brazil, being Pyroglyphidae, Glycyphagidae and Cheyletidae the most important families found. This paper is an annotated bibliography that will lead to a better knowledge of house dust mite fauna in Brazil.
A rapid, sensitive and specific method to quantify nevirapine in human plasma using dibenzepine a... more A rapid, sensitive and specific method to quantify nevirapine in human plasma using dibenzepine as the internal standard (IS) was developed and validated. The method employed a liquid–liquid extraction. The analyte and the IS were chromatographed on a C18 analytical column, (150 × 4.6 mm i.d. 4 µm) and analyzed by tandem mass spectrometry in the multiple reaction monitoring mode. The method had a chromatographic run time of 5.0 min and a linear calibration curve over the range 10–5000 ng ml−1 (r2 > 0.9970). The between‐run precision, based on the relative standard deviation for replicate quality controls was 1.3% (30 ng ml−1), 2.8% (300 ng ml−1) and 3.6% (3000 ng ml−1). The between‐run accuracy was 4.0, 7.0 and 6.2% for the above‐mentioned concentrations, respectively. This method was employed in a bioequivalence study of two nevirapine tablet formulations (Nevirapina from Far‐Manguinhos, Brazil, as a test formulation, and Viramune from Boehringer Ingelheim do Brasil Química e Fa...
An analytical method based on liquid chromatography with positive ion electrospray ionization (ES... more An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection (LC-MS/MS) was developed for the determination of Verapamil in human plasma using Metoprolol as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid-liquid extraction and chromatographed on a C(8) analytical column. The mobile phase consisted of methanol-water (70:30; v/v)+12 mM formic acid. The method had a chromatographic total run time of 3.5 min and was linear within the range 1.00-500 ng/mL. Detection was carried out on a Micromass Quattro Ultima tandem mass spectrometer by multiple reaction monitoring (MRM). The intra-run imprecision was less than 5.1% calculated from the quality control (QC) samples, and 16.3% from the limit of quantification (LOQ). The accuracy determined from QC samples were between 92.9 and 103.1%, and 95.2 and 115.3% from LOQ. Concerning the inter-batch analysis, the imprecision was less than 5.8% and 17.3% from QC samples and LOQ, respectively. The accuracy varied between 98.2 and 100.8% from QC and it was 103.1% from LOQ. The protocol herein described was employed in a bioequivalence study of two tablet formulations of Verapamil.
A method based on LC-MS-MS is described for the determination of methyldopa in human plasma using... more A method based on LC-MS-MS is described for the determination of methyldopa in human plasma using dopa-phenyl-D3 as the internal standard. The method has a chromatographic run time of 5.5 min and was linear in the range of 20-5000 ng / ml. The limit of quantitation was 20 ng / ml, the intra-day precisions were 7.3, 5.4 and 4.3% and the intra-day accuracies were 28.0, 21.3 and 22.0% for 30, 600 and 3000 ng / ml, respectively. The inter-day precisions were 7.7, 0.5 and 0.7% and the inter-day accuracies were 0.2, 21.1 and 22.3%, respectively, for the above concentrations. This method was employed in a bioequivalence study of two tablet formulations of methyldopa.
An analytical method based on liquid chromatography with positive ion electrospray ionization (ES... more An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection was developed for the determination of lansoprazole in human plasma using omeprazole as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid-liquid extraction using diethyl-ether-dichloromethane (70:30; v / v) and chromatographed on a C analytical column. The mobile phase 18 consisted of acetonitrile-water (90:10; v / v)110 mM formic acid. The method has a chromatographic total run time of 5 min and was linear within the range 2.5-2000 ng / ml. Detection was carried out on a Micromass triple quadrupole tandem mass spectrometer by Multiple Reaction Monitoring (MRM). The intra-and inter-run precision, calculated from quality control (QC) samples, was less than 3.4%. The accuracy as determined from QC samples was less than 9%. The method herein described was employed in a bioequivalence study of two capsule formulations of lansoprazole.
Int. Journal of Clinical Pharmacology and Therapeutics, 2004
The aim of study was to compare the bioavailability of 2 cyclosporine capsule formulations (100 m... more The aim of study was to compare the bioavailability of 2 cyclosporine capsule formulations (100 mg; Sigmasporin Microoral from Novaquímica Divisão Nature&amp;amp;amp;amp;#39;s Plus Farmacêutica Ltd., Brazil, as test formulation and Sandimmune Neoral from Novartis Biociências S.A., Brazil, as reference formulation) in 24 healthy male volunteers. The study was open, randomized, with a 2-period crossover, a 1-week washout interval between doses. Blood samples were obtained over a 12-hour interval after each oral administration of cyclosporine (2 capsules of 100 mg of each formulation). Cyclosporine blood concentrations were quantified using a fluorescence polarization immunoassay (FPIA) method provided by Abbott Axsym System and Cyclo-Trac SP. Whole-blood radioimmuoassay (RIA) kit was provided by DiaSorin. These assays provided concentration-time curves for cyclosporine in blood concentration from which the following pharmacokinetic parameters were obtained: AUC(last), AUC(inf), Cmax. Geometric mean and 90% confidence intervals (CI) of Microoral/Neoral as percent ratios were 94.5% (90.8-98.4%) for AUC(last), 93.8% (89.7-98.1%) for AUC(inf), and 98.1% (94.5-101.8%) for Cmax when cyclosporine was determined using FPIA and 96.1% (91.9 to 100.6%) for AUC(last), 95.2% (90.2-100.5%) for AUC(inf), and 99.4% (96.4-102.4%) for Cmax using RIA. Since the 90% CI for Cmax, AUC(last) and AUC(inf) ratio were within the 80-125% interval proposed by US-FDA, it is concluded that Sigmasporin Microoral 100 mg capsule formulation is bioequivalent to Sandimmune Neoral 100 mg capsule formulation with regard to both rate and the extent of absorption.
In order to compare patterns of indoor air perception, including perceptions of temperature, air ... more In order to compare patterns of indoor air perception, including perceptions of temperature, air movement, indoor air quality (IAQ), mental concentration, and comfort, 33 subjects either with persistent allergic rhinitis or controls were exposed to different temperatures and constant relative humidity in an experimental office environment. Results were obtained by means of a self-administered visual analogue scale, analyzed using mean score comparisons and principal component analysis. At 14 degrees C, the rhinitis group reported higher scores for sensations of air dryness than controls. At 18 degrees C, in the rhinitis group, there was a correlation between dry, stagnant air, and difficult mental concentration. This group also correlated heat, dry air, and poor IAQ, in contrast to the control group, which correlated comfort, easy mental concentration, and freshness. At 22 degrees C, the rhinitis group correlated heat, dryness, stagnant air, and overall discomfort. This group also correlated non-dry air, freshness, and comfort, whereas the control group correlated heat, humidity, good indoor air, freshness, and comfort. This study suggests that the rhinitis group perceives indoor temperatures of 14 degrees C as dryer than controls do, and that at 18 and 22 degrees C this group positively correlates different adverse perceptions of IAQ. By means of a self-administered questionnaire in an experimental condition, the present study compares subjective patterns of indoor air perception from individuals with respiratory allergy (allergic rhinitis) to control individuals. It reports different patterns of perception of indoor air quality (IAQ) between the two groups, suggesting that allergic individuals could have different IAQ perception.
To evaluate the association of heating, ventilation and airconditioning systems (HVAC) and respir... more To evaluate the association of heating, ventilation and airconditioning systems (HVAC) and respiratory symptoms in a tropical city, self-administered questionnaires were given to 2000 individuals working in air-conditioned office buildings and to 500 control workers in naturally ventilated buildings. Reported symptoms from the two populations were analyzed using chi-square tests, univariate and multiple logistic regressions models. Symptoms were the outcome variable and the odds ratios were adjusted by gender, age, accumulated work time, smoking habits and atopic background. There was a 79.8% response rate and there was a positive association of nasal symptoms (odds ratio, OR ¼ 1.59, 95% confidence interval, CI ¼ 1.11-2.28), naso-ocular symptoms (OR ¼ 1.58, 95% CI ¼ 1.05-2.38), persistent cough (OR ¼ 3.04, 95% CI ¼ 2.00-4.63) sinusitis symptoms (OR ¼ 1.85, 95% CI ¼ 1.27-2.71) and building-related worsening of the symptoms (OR ¼ 4.92, 95% CI ¼ 2.93-8.27) with working in air-conditioned buildings. In conclusion, our study suggests that artificial airconditioning is a matter of concern for respiratory symptoms in cities with hot and humid climate.
Quantifizierung von Pantoprazol in Humanplasma mit LC-MS-MS und Lansoprazol als internem Standard... more Quantifizierung von Pantoprazol in Humanplasma mit LC-MS-MS und Lansoprazol als internem Standard Es wurde ein Analyseverfahren zur Quantifizierung von Pantoprazol (CAS 102625-70-7) in Humanplasma mit Flüs-Summary An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection was developed for the determination of pantoprazole (CAS 102625-70-7) in human plasma using lansoprazole (CAS 103577-45-3) as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid/liquid extraction using diethyl-ether/dichloromethane (70:30; v/v) and chromatographed on a C 8 analytical column. The mobile phase consisted of acetonitrile/ water/methanol (57:25:18; v/v/v) + 10 mmol/l acetic acid + 20 mmol/l ammonium acetate. The method has a chromatographic total run time of 4.5 min and was linear within the range 5.0−5,000 ng/ mL. Detection was performed on a triple quadrupole tandem mass spectrometer by Multiple Reaction Monitoring (MRM).
Page 1. Clinical toxicology study of a herbal medicinal extract of Aconitum na-pellus, Nasturtium... more Page 1. Clinical toxicology study of a herbal medicinal extract of Aconitum na-pellus, Nasturtium officinale, Myroxylon balsamum, Mikania glomerata, Cephaelis ipecacuanha and Polygala senega (Melagrião®) in healthy vol-unteers ...
Page 1. Clinical toxicology study of a laxative herbal medicinal extract in healthy volunteers Ce... more Page 1. Clinical toxicology study of a laxative herbal medicinal extract in healthy volunteers Celso H. Oliveira¹*, Maria Elisabete A. Moraes³, Manoel O. Moraes³, Fernando AF Be-zerra³, Karla F. Deud-José and Gilberto De Nucci² ...
Objective—To assess the bioequivalence of two amlodipine tablet formulations (Amlodipine® 5 mg ta... more Objective—To assess the bioequivalence of two amlodipine tablet formulations (Amlodipine® 5 mg tablet from Merck S.A. Indústrias Químicas, Brazil as test formulation and Norvasc® 5 mg tablet from Laboratórios Pfizer Ltd., Brazil as reference formulation) in 24 healthy volunteers of both sexes.Methods—The study was conducted using an open, randomized two‐period crossover design with a 4‐week washout interval. Plasma samples were obtained over a 144 h period. Plasma amlodipine concentrations were analyzed by combined liquid chromatography coupled to tandem mass spectrometry (LC‐MS‐MS) with positive ion electrospray ionization using multiple reaction monitoring (MRM). From the amlodipine plasma concentration vs time curves, the following pharmacokinetic parameters were obtained: AUClast, AUC0–inf and Cmax. The statistical interval proposed was 80–125% according to the US Food and Drug Administration Agency.Results—The limit of quantification was 0.1 ng/ml for plasma amlodipine analysis...
Objective. To compare the bioavailability of two cefadroxil capsule (500 mg) formulations (Cefadr... more Objective. To compare the bioavailability of two cefadroxil capsule (500 mg) formulations (Cefadroxila from Eurofarma Laborató rios Ltd, Brazil, as test formulation and Cefamox ® from Bristol-Myers Squibb, Brazil S.A. as reference formulation) in 24 volunteers of both sexes. Material and methods. The study was conducted open with randomized two-period crossover design and a 1-week washout period. Plasma samples were obtained over a 12-h interval. Cefadroxil concentrations were analysed by combined reversed-phase liquid chromatography and tandem mass spectrometry (LC-MS-MS) with positive ion electrospray ionization using a selected ion monitoring method. From the cefadroxil plasma concentration versus time curves the following pharmacokinetic parameters were obtained: AUC last , AUC 0-and C max. Results. Geometric mean of Cefadroxila/Cefamox ® 500 mg individual percent ratio was 103.97% for AUC last , 104.08% for AUC 0-and 95.23% for C max. The 90% confidence intervals (CI) were 98.14-110.16%, 98.37-110.12%, and 85.59-105.96%, respectively. Conclusion. Since the 90% CI for C max , AUC last and AUC 0-were within the 80-125% interval proposed by the Food and Drug Administration, it was concluded that the Cefadroxila 500 mg capsule was bioequivalent to the Cefamox ® 500 mg capsule, according to both the rate and extent of absorption.
The aim of this study was to investigate the mite fauna present in 33 living room and 22 bedroom ... more The aim of this study was to investigate the mite fauna present in 33 living room and 22 bedroom curtain dust samples from 41 different homes in the southern Brazilian city of Campinas, SP. A total of 148 mite bodies were found. Of these, 83 were found in living-room curtain samples (56.1% of total) and 65 were in bedroom curtain dust samples (43.9%). The most frequently observed mite suborders were: Acaridida (n = 79; 53.4%), Actinedida (n = 53; 35.8%), Oribatida (n = 14; 9.5%), and Gamasida (n = 2; 1.3%). The most frequent families were Pyroglyphidae (n = 61; 41.2%), Eriophyidae (n = 25; 16.9%), Tarsonemidae (n = 15; 10.1%), and Glycyphagidae (n = 13; 8.8%). No statistical difference was observed between the number of mites found in the samples from living room and bedroom curtains.O intuito deste estudo foi avaliar as espécies acarinas encontradas em amostras de poeira de cortinas presentes em 33 salas e 22 quartos de um total de 41 domicílios na cidade de Campinas, SP. Foram enc...
International journal of clinical pharmacology and therapeutics, 2004
To compare the bioavailability of a fluconazole 150 mg capsule formulation from Laboratório Teuto... more To compare the bioavailability of a fluconazole 150 mg capsule formulation from Laboratório Teuto Brasileiro Ltd., Brazil (test formulation), and Zoltec 150 mg capsule from Laboratórios Pfizer Ltd., Brazil (reference formulation), in 24 volunteers of both sexes. The study was conducted open with randomized 2-period crossover design and a 2-week washout period. Plasma samples were obtained over a 168-hour interval. Fluconazole concentrations were analyzed by combined reversed-phase liquid chromatography and tandem mass spectrometry (LC/MS/MS) with positive ion electrospray ionization using selected ion monitoring method. From the fluconazole plasma concentration vs. time curves the following pharmacokinetic parameters were obtained: AUC(last), AUC(0-inf) and C(max). Geometric mean of fluconazole/Zoltec 150 mg individual percent ratio was 102.6% for AUC(last), 102.2% for AUC(0-inf) and 109.4% for C(max). The 90% confidence intervals were 97.3-108.2%, 97.0-107.8%, and 103.1-116.0%, res...
The aim of this study was to investigate the mite fauna present in 33 living room and 22 bedroom ... more The aim of this study was to investigate the mite fauna present in 33 living room and 22 bedroom curtain dust samples from 41 different homes in the southern Brazilian city of Campinas, SP. A total of 148 mite bodies were found. Of these, 83 were found in living-room curtain samples (56.1% of total) and 65 were in bedroom curtain dust samples (43.9%). The most frequently observed mite suborders were: Acaridida (n = 79; 53.4%), Actinedida (n = 53; 35.8%), Oribatida (n = 14; 9.5%), and Gamasida (n = 2; 1.3%). The most frequent families were Pyroglyphidae (n = 61; 41.2%), Eriophyidae (n = 25; 16.9%), Tarsonemidae (n = 15; 10.1%), and Glycyphagidae (n = 13; 8.8%). No statistical difference was observed between the number of mites found in the samples from living room and bedroom curtains.
A method based on liquid chromatography with positive ion electrospray ionization and tandem mass... more A method based on liquid chromatography with positive ion electrospray ionization and tandem mass spectrometry is described for the determination of terbinafine in human plasma using naftifine as internal standard. The method has a chromatographic run time of 5 minutes and was linear in the range 1.0 to 2000 ng/mL. The limit of quantification was 1.0 ng/mL; the intraday precision was 3.6%, 3.8%, 3.5%, and 4.1%; and the intraday accuracy was -2.7%, 7.7%, 4.8%, and -2.7% for 5.0, 80.0, 250.0, and 1500.0 ng/mL, respectively. The interday precision was 4.9%, 1.7%, 2.4%, and 4.6% and the interday accuracy was 0.3%, 5.8%, 6.5%, and -1.4% for the same concentrations. This method was used in a bioequivalence study of two tablet formulations of terbinafine. Twenty-four healthy volunteers (both sexes) received a single oral dose of terbinafine (250 mg) in an open, randomized, two-period crossover study. The 90% CI of geometric mean ratios between Terbinafina (Medley S/A Indústria Farmacêutica, Campinas, Brazil) and Lamisil (Novartis Biociências S/A, São Paulo, Brazil) were 90.5% to 110.0% for C max, 92.2% to 108.1% for AUC last, and 91.3% to 107.5% for AUC 0-inf. Because the 90% CI for the above-mentioned parameters were included in the 80% to 125% interval proposed by the US FDA, the two formulations were considered bioequivalent in terms of rate and extent of absorption.
In Brazil, a herbal medicinal extract named Catuama containing a mixture of Paullinia cupana (gua... more In Brazil, a herbal medicinal extract named Catuama containing a mixture of Paullinia cupana (guarana; Sapindaceae), Trichilia catigua (catuaba; Meliaceae), Ptychopetalum olacoides (muirapuama; Olacaceae) and Zingiber officinale (ginger; Zingiberaceae) is used as a body stimulant, energetic, tonic and aphrodisiac. The present study investigated the chronic administration of 25 mL Catuama twice a day during 28 days for any toxic effect on healthy human volunteers of both sexes. No severe adverse reactions or haematological and biochemical changes were reported.
The aim of this study was to investigate the mite fauna in mattresses dust samples from cribs or ... more The aim of this study was to investigate the mite fauna in mattresses dust samples from cribs or beds in the south Brazilian city of Londrina, State of Paraná. A total of 133 dust samples from upper and lower mattress surfaces, and bed frames were aspirated once from 38 dwellings (18 cribs and 21 beds), and one day nursery (six cribs). A total of 758 mite bodies were counted in slides: 233 (30.7%) from cribs and 525 (69.3%) from beds (p < 0.001). House dust mites – mainly Dermatophagoides pteronyssinus, represented 72% and 84% of total mite count in crib and bed dust samples, respectively. The mean HDM body concentration in crib or bed slides were, respectively, 289.9 ± 136.7 and 875.0 ± 183.6 mites/g. Statistical analysis showed a significantly higher mite bodies count on lower mattress surface compared with upper surface in bed samples only (p = 0.025). Data herein show that cribs like mattress have sufficient mite bodies to cause sensitization to humans. The use of mattress co...
House dust mites have been reported to be the most important allergen in human dwellings. Several... more House dust mites have been reported to be the most important allergen in human dwellings. Several articles had already shown the presence of different mite species at homes in Brazil, being Pyroglyphidae, Glycyphagidae and Cheyletidae the most important families found. This paper is an annotated bibliography that will lead to a better knowledge of house dust mite fauna in Brazil.
A rapid, sensitive and specific method to quantify nevirapine in human plasma using dibenzepine a... more A rapid, sensitive and specific method to quantify nevirapine in human plasma using dibenzepine as the internal standard (IS) was developed and validated. The method employed a liquid–liquid extraction. The analyte and the IS were chromatographed on a C18 analytical column, (150 × 4.6 mm i.d. 4 µm) and analyzed by tandem mass spectrometry in the multiple reaction monitoring mode. The method had a chromatographic run time of 5.0 min and a linear calibration curve over the range 10–5000 ng ml−1 (r2 > 0.9970). The between‐run precision, based on the relative standard deviation for replicate quality controls was 1.3% (30 ng ml−1), 2.8% (300 ng ml−1) and 3.6% (3000 ng ml−1). The between‐run accuracy was 4.0, 7.0 and 6.2% for the above‐mentioned concentrations, respectively. This method was employed in a bioequivalence study of two nevirapine tablet formulations (Nevirapina from Far‐Manguinhos, Brazil, as a test formulation, and Viramune from Boehringer Ingelheim do Brasil Química e Fa...
An analytical method based on liquid chromatography with positive ion electrospray ionization (ES... more An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection (LC-MS/MS) was developed for the determination of Verapamil in human plasma using Metoprolol as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid-liquid extraction and chromatographed on a C(8) analytical column. The mobile phase consisted of methanol-water (70:30; v/v)+12 mM formic acid. The method had a chromatographic total run time of 3.5 min and was linear within the range 1.00-500 ng/mL. Detection was carried out on a Micromass Quattro Ultima tandem mass spectrometer by multiple reaction monitoring (MRM). The intra-run imprecision was less than 5.1% calculated from the quality control (QC) samples, and 16.3% from the limit of quantification (LOQ). The accuracy determined from QC samples were between 92.9 and 103.1%, and 95.2 and 115.3% from LOQ. Concerning the inter-batch analysis, the imprecision was less than 5.8% and 17.3% from QC samples and LOQ, respectively. The accuracy varied between 98.2 and 100.8% from QC and it was 103.1% from LOQ. The protocol herein described was employed in a bioequivalence study of two tablet formulations of Verapamil.
A method based on LC-MS-MS is described for the determination of methyldopa in human plasma using... more A method based on LC-MS-MS is described for the determination of methyldopa in human plasma using dopa-phenyl-D3 as the internal standard. The method has a chromatographic run time of 5.5 min and was linear in the range of 20-5000 ng / ml. The limit of quantitation was 20 ng / ml, the intra-day precisions were 7.3, 5.4 and 4.3% and the intra-day accuracies were 28.0, 21.3 and 22.0% for 30, 600 and 3000 ng / ml, respectively. The inter-day precisions were 7.7, 0.5 and 0.7% and the inter-day accuracies were 0.2, 21.1 and 22.3%, respectively, for the above concentrations. This method was employed in a bioequivalence study of two tablet formulations of methyldopa.
An analytical method based on liquid chromatography with positive ion electrospray ionization (ES... more An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection was developed for the determination of lansoprazole in human plasma using omeprazole as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid-liquid extraction using diethyl-ether-dichloromethane (70:30; v / v) and chromatographed on a C analytical column. The mobile phase 18 consisted of acetonitrile-water (90:10; v / v)110 mM formic acid. The method has a chromatographic total run time of 5 min and was linear within the range 2.5-2000 ng / ml. Detection was carried out on a Micromass triple quadrupole tandem mass spectrometer by Multiple Reaction Monitoring (MRM). The intra-and inter-run precision, calculated from quality control (QC) samples, was less than 3.4%. The accuracy as determined from QC samples was less than 9%. The method herein described was employed in a bioequivalence study of two capsule formulations of lansoprazole.
Int. Journal of Clinical Pharmacology and Therapeutics, 2004
The aim of study was to compare the bioavailability of 2 cyclosporine capsule formulations (100 m... more The aim of study was to compare the bioavailability of 2 cyclosporine capsule formulations (100 mg; Sigmasporin Microoral from Novaquímica Divisão Nature&amp;amp;amp;amp;#39;s Plus Farmacêutica Ltd., Brazil, as test formulation and Sandimmune Neoral from Novartis Biociências S.A., Brazil, as reference formulation) in 24 healthy male volunteers. The study was open, randomized, with a 2-period crossover, a 1-week washout interval between doses. Blood samples were obtained over a 12-hour interval after each oral administration of cyclosporine (2 capsules of 100 mg of each formulation). Cyclosporine blood concentrations were quantified using a fluorescence polarization immunoassay (FPIA) method provided by Abbott Axsym System and Cyclo-Trac SP. Whole-blood radioimmuoassay (RIA) kit was provided by DiaSorin. These assays provided concentration-time curves for cyclosporine in blood concentration from which the following pharmacokinetic parameters were obtained: AUC(last), AUC(inf), Cmax. Geometric mean and 90% confidence intervals (CI) of Microoral/Neoral as percent ratios were 94.5% (90.8-98.4%) for AUC(last), 93.8% (89.7-98.1%) for AUC(inf), and 98.1% (94.5-101.8%) for Cmax when cyclosporine was determined using FPIA and 96.1% (91.9 to 100.6%) for AUC(last), 95.2% (90.2-100.5%) for AUC(inf), and 99.4% (96.4-102.4%) for Cmax using RIA. Since the 90% CI for Cmax, AUC(last) and AUC(inf) ratio were within the 80-125% interval proposed by US-FDA, it is concluded that Sigmasporin Microoral 100 mg capsule formulation is bioequivalent to Sandimmune Neoral 100 mg capsule formulation with regard to both rate and the extent of absorption.
In order to compare patterns of indoor air perception, including perceptions of temperature, air ... more In order to compare patterns of indoor air perception, including perceptions of temperature, air movement, indoor air quality (IAQ), mental concentration, and comfort, 33 subjects either with persistent allergic rhinitis or controls were exposed to different temperatures and constant relative humidity in an experimental office environment. Results were obtained by means of a self-administered visual analogue scale, analyzed using mean score comparisons and principal component analysis. At 14 degrees C, the rhinitis group reported higher scores for sensations of air dryness than controls. At 18 degrees C, in the rhinitis group, there was a correlation between dry, stagnant air, and difficult mental concentration. This group also correlated heat, dry air, and poor IAQ, in contrast to the control group, which correlated comfort, easy mental concentration, and freshness. At 22 degrees C, the rhinitis group correlated heat, dryness, stagnant air, and overall discomfort. This group also correlated non-dry air, freshness, and comfort, whereas the control group correlated heat, humidity, good indoor air, freshness, and comfort. This study suggests that the rhinitis group perceives indoor temperatures of 14 degrees C as dryer than controls do, and that at 18 and 22 degrees C this group positively correlates different adverse perceptions of IAQ. By means of a self-administered questionnaire in an experimental condition, the present study compares subjective patterns of indoor air perception from individuals with respiratory allergy (allergic rhinitis) to control individuals. It reports different patterns of perception of indoor air quality (IAQ) between the two groups, suggesting that allergic individuals could have different IAQ perception.
To evaluate the association of heating, ventilation and airconditioning systems (HVAC) and respir... more To evaluate the association of heating, ventilation and airconditioning systems (HVAC) and respiratory symptoms in a tropical city, self-administered questionnaires were given to 2000 individuals working in air-conditioned office buildings and to 500 control workers in naturally ventilated buildings. Reported symptoms from the two populations were analyzed using chi-square tests, univariate and multiple logistic regressions models. Symptoms were the outcome variable and the odds ratios were adjusted by gender, age, accumulated work time, smoking habits and atopic background. There was a 79.8% response rate and there was a positive association of nasal symptoms (odds ratio, OR ¼ 1.59, 95% confidence interval, CI ¼ 1.11-2.28), naso-ocular symptoms (OR ¼ 1.58, 95% CI ¼ 1.05-2.38), persistent cough (OR ¼ 3.04, 95% CI ¼ 2.00-4.63) sinusitis symptoms (OR ¼ 1.85, 95% CI ¼ 1.27-2.71) and building-related worsening of the symptoms (OR ¼ 4.92, 95% CI ¼ 2.93-8.27) with working in air-conditioned buildings. In conclusion, our study suggests that artificial airconditioning is a matter of concern for respiratory symptoms in cities with hot and humid climate.
Quantifizierung von Pantoprazol in Humanplasma mit LC-MS-MS und Lansoprazol als internem Standard... more Quantifizierung von Pantoprazol in Humanplasma mit LC-MS-MS und Lansoprazol als internem Standard Es wurde ein Analyseverfahren zur Quantifizierung von Pantoprazol (CAS 102625-70-7) in Humanplasma mit Flüs-Summary An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection was developed for the determination of pantoprazole (CAS 102625-70-7) in human plasma using lansoprazole (CAS 103577-45-3) as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid/liquid extraction using diethyl-ether/dichloromethane (70:30; v/v) and chromatographed on a C 8 analytical column. The mobile phase consisted of acetonitrile/ water/methanol (57:25:18; v/v/v) + 10 mmol/l acetic acid + 20 mmol/l ammonium acetate. The method has a chromatographic total run time of 4.5 min and was linear within the range 5.0−5,000 ng/ mL. Detection was performed on a triple quadrupole tandem mass spectrometer by Multiple Reaction Monitoring (MRM).
Page 1. Clinical toxicology study of a herbal medicinal extract of Aconitum na-pellus, Nasturtium... more Page 1. Clinical toxicology study of a herbal medicinal extract of Aconitum na-pellus, Nasturtium officinale, Myroxylon balsamum, Mikania glomerata, Cephaelis ipecacuanha and Polygala senega (Melagrião®) in healthy vol-unteers ...
Page 1. Clinical toxicology study of a laxative herbal medicinal extract in healthy volunteers Ce... more Page 1. Clinical toxicology study of a laxative herbal medicinal extract in healthy volunteers Celso H. Oliveira¹*, Maria Elisabete A. Moraes³, Manoel O. Moraes³, Fernando AF Be-zerra³, Karla F. Deud-José and Gilberto De Nucci² ...
Objective—To assess the bioequivalence of two amlodipine tablet formulations (Amlodipine® 5 mg ta... more Objective—To assess the bioequivalence of two amlodipine tablet formulations (Amlodipine® 5 mg tablet from Merck S.A. Indústrias Químicas, Brazil as test formulation and Norvasc® 5 mg tablet from Laboratórios Pfizer Ltd., Brazil as reference formulation) in 24 healthy volunteers of both sexes.Methods—The study was conducted using an open, randomized two‐period crossover design with a 4‐week washout interval. Plasma samples were obtained over a 144 h period. Plasma amlodipine concentrations were analyzed by combined liquid chromatography coupled to tandem mass spectrometry (LC‐MS‐MS) with positive ion electrospray ionization using multiple reaction monitoring (MRM). From the amlodipine plasma concentration vs time curves, the following pharmacokinetic parameters were obtained: AUClast, AUC0–inf and Cmax. The statistical interval proposed was 80–125% according to the US Food and Drug Administration Agency.Results—The limit of quantification was 0.1 ng/ml for plasma amlodipine analysis...
Objective. To compare the bioavailability of two cefadroxil capsule (500 mg) formulations (Cefadr... more Objective. To compare the bioavailability of two cefadroxil capsule (500 mg) formulations (Cefadroxila from Eurofarma Laborató rios Ltd, Brazil, as test formulation and Cefamox ® from Bristol-Myers Squibb, Brazil S.A. as reference formulation) in 24 volunteers of both sexes. Material and methods. The study was conducted open with randomized two-period crossover design and a 1-week washout period. Plasma samples were obtained over a 12-h interval. Cefadroxil concentrations were analysed by combined reversed-phase liquid chromatography and tandem mass spectrometry (LC-MS-MS) with positive ion electrospray ionization using a selected ion monitoring method. From the cefadroxil plasma concentration versus time curves the following pharmacokinetic parameters were obtained: AUC last , AUC 0-and C max. Results. Geometric mean of Cefadroxila/Cefamox ® 500 mg individual percent ratio was 103.97% for AUC last , 104.08% for AUC 0-and 95.23% for C max. The 90% confidence intervals (CI) were 98.14-110.16%, 98.37-110.12%, and 85.59-105.96%, respectively. Conclusion. Since the 90% CI for C max , AUC last and AUC 0-were within the 80-125% interval proposed by the Food and Drug Administration, it was concluded that the Cefadroxila 500 mg capsule was bioequivalent to the Cefamox ® 500 mg capsule, according to both the rate and extent of absorption.
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