Papers by Catherine J A Deflandre
European Heart Journal, Apr 19, 2006
Aims The use of doxorubicin (DOX) as a chemotherapeutic agent is limited by cardiac injury. Ilopr... more Aims The use of doxorubicin (DOX) as a chemotherapeutic agent is limited by cardiac injury. Iloprost, a stable synthetic analogue of prostacyclin, has previously been shown to protect against DOX-induced cardiomyocyte injury in vitro. Here, we addressed whether iloprost is cardioprotective in vivo and whether it compromises the anti-tumour efficacy of DOX. Methods and results Lewis Lung Carcinoma cells were implanted subcutaneously in the flank of C57BL/6 mice. DOX treatment was commenced from when tumours became visible. Iloprost was administered from prior to DOX treatment until sacrifice. Echocardiography and invasive haemodynamic measurements were performed immediately before sacrifice. As expected, DOX induced cardiac cell apoptosis and cardiac dysfunction, both of which were attenuated by iloprost. Also, iloprost alone had no effect on tumor growth and indeed, did not alter the DOX-induced suppression of this growth. Conclusion In a murine model, iloprost attenuated the acute cardiac injury and dysfunction induced by DOX therapy without compromising its chemotherapeutic effect.
Veterinary Anaesthesia and Analgesia, 2008
Objective To compare the performance of the Surgivet Non-Invasive Blood Pressure (NIBP) monitor V... more Objective To compare the performance of the Surgivet Non-Invasive Blood Pressure (NIBP) monitor V60046 with an invasive blood pressure (IBP) technique in anaesthetized dogs. Study design A prospective study. Animals Thirty-four dogs, anaesthetized for a variety of procedures.
Journal of Comparative Pathology, Oct 1, 2002
Journal of Comparative Pathology, 2002
Feline osteoclastic resorptive lesions (FORL) of the teeth are common in cats, and lead to pain, ... more Feline osteoclastic resorptive lesions (FORL) of the teeth are common in cats, and lead to pain, destruction of the periodontal ligament, and tooth loss. The expression of interleukin (IL)-1β and IL-6 mRNA was higher in teeth with FORL than in normal teeth (P<0·01 and P<0·001, respectively), but no such differences were found between pathological and normal gingival tissue samples. There were no differences between teeth affected with FORL and normal teeth in respect of the expression of receptor activator of NFκB ligand (RANKL) mRNA or osteoprotegerin (OPG) mRNA. However, OPG mRNA expression was higher in gingival tissue associated with teeth affected with FORL than in normal gingival tissue (P<0·05), whereas the reverse was true of RANKL mRNA expression (P<0·05). OPG mRNA expression was significantly higher in teeth than in femoral and alveolar bone (P<0·001). RANKL and OPG mRNAs were detected in all tissues examined. The data suggest that the elevated expression of IL-lβ and IL-6 mRNA plays a role in the mediation of osteoclast activity in advanced FORL. In contrast, OPG and RANKL do not appear to regulate osteoclasts in advanced disease. The results also suggest that OPG and RANKL mRNA play a role in mediating inflammatory responses in gingival cells, and that OPG has an inhibiting effect on tooth resorption.
Aims The use of doxorubicin (DOX) as a chemotherapeutic agent is limited by cardiac injury. Ilopr... more Aims The use of doxorubicin (DOX) as a chemotherapeutic agent is limited by cardiac injury. Iloprost, a stable synthetic analogue of prostacyclin, has previously been shown to protect against DOX-induced cardiomyocyte injury in vitro. Here, we addressed whether iloprost is cardioprotective in vivo and whether it compromises the anti-tumour efficacy of DOX. Methods and results Lewis Lung Carcinoma cells were implanted subcutaneously in the flank of C57BL/6 mice. DOX treatment was commenced from when tumours became visible. Iloprost was administered from prior to DOX treatment until sacrifice. Echocardiography and invasive haemodynamic measurements were performed immediately before sacrifice. As expected, DOX induced cardiac cell apoptosis and cardiac dysfunction, both of which were attenuated by iloprost. Also, iloprost alone had no effect on tumor growth and indeed, did not alter the DOX-induced suppression of this growth.
To determine the role of cyclooxygenase (COX)-2 in anthracycline-induced cardiac toxicity, we adm... more To determine the role of cyclooxygenase (COX)-2 in anthracycline-induced cardiac toxicity, we administered doxorubicin (DOX) to mice with genetic disruption of COX-2 (COX-2 -/-). After treatment with DOX, COX-2 -/mice had increased cardiac dysfunction and cardiac cell apoptosis as compared to DOX-treated wild-type mice.
Veterinary Anaesthesia and Analgesia, 2007
Objective To compare the performance of the Surgivet Non-Invasive Blood Pressure (NIBP) monitor ... more Objective To compare the performance of the Surgivet Non-Invasive Blood Pressure (NIBP) monitor V60046 with an invasive blood pressure (IBP) technique in anaesthetized dogs.Study design A prospective study.Animals Thirty-four dogs, anaesthetized for a variety of procedures.Methods Various anaesthetic protocols were used. Invasive blood pressure measurement was made using a catheter in the femoral or the pedal artery. A cuff was placed on the contralateral limb to allow non invasive measurements. Recordings of arterial blood pressures (ABPs) were taken at simultaneous times for a range of pressures. For analysis, three pressure levels were determined: high [systolic blood pressure (SAP) > 121 mmHg], normal (91 mmHg < SAP < 120 mmHg) and low (SAP < 90 mmHg). Comparisons between invasive and non invasive measurements were made using Bland-Altmann analysis.Results The NIBP monitor consistently underestimated blood pressure at all levels. The lowest biases and greatest precision were obtained at low and normal pressure levels for SAP and mean arterial pressure (MAP). At low blood pressure levels, the biases ± 95% confidence interval (CI) were 1.9 ± 2.96 mmHg (SAP), 8.3 ± 2.41 mmHg diastolic arterial pressure (DAP) and 3.5 ± 2.09 mmHg (MAP). At normal blood pressure levels, biases and CI were: 1.2 ± 2.13 mmHg (SAP), 5.2 ± 2.32 mmHg (DAP) and 2.1 ± 1.54 mmHg (MAP). At high blood pressure levels, the biases and CI were 22.7 ± 5.85 mmHg (SAP), 5.5 ± 3.13 mmHg (DAP) and 9.4 ± 3.52 mmHg (MAP). In 90.6% of cases of hypotension (MAP < 70 mmHg), the low blood pressure was correctly diagnosed by the Surgivet.Conclusions Measurement of blood pressure with the indirect monitor allowed detection of hypotension using either SAP or MAP. The most accurate readings were determined for MAP at hypotensive and normal levels. The monitor lacked accuracy at high pressures.Clinical relevance When severe challenges to the cardiovascular system are anticipated, an invasive method of recording ABP is preferable. For routine usage, the Surgivet monitor provided a reliable and safe method of NIBP monitoring in dogs, thereby contributing to the safety of anaesthesia by providing accurate information about the circulation.
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Papers by Catherine J A Deflandre