O mapeamento morfológico é uma importante ferramenta na interpretação de processos, materiais e f... more O mapeamento morfológico é uma importante ferramenta na interpretação de processos, materiais e formas do relevo regional. Sobretudo em Unidades de Conservação, auxilia no planejamento e gestão ambiental das áreas protegidas. Nesse contexto, o principal objetivo desse trabalho é caracterizar os compartimentos morfológicos do Parque Nacional da Serra do Cipó a partir da medição de parâmetros morfométricos. Os procedimentos metodológicos envolvem a sobreposição de dados cartográficos, a elaboração de perfis topográficos, a identificação de rupturas de declive e, finalmente, medições morfométricas. Os resultados mostram que o Parque Nacional da Serra do Cipó pode ser dividido em onze compartimentos morfológicos na escala de 1:50.000, demonstrando a importância da morfometria na individualização de unidades do relevo. Espera-se que esses resultados e os avanços metodológicos desse trabalho possam colaborar para pesquisas futuras assim como para a gestão de Unidades de Conservação na Ser...
This study aimed to characterize the susceptibility profile to antibiotics and biofilm formation ... more This study aimed to characterize the susceptibility profile to antibiotics and biofilm formation of Gram-negative bacterial isolates obtained from the oral cavity of the black-and-white ruffed lemur (Varecia variegata). From eight individuals from a zoo located in Portugal, samples of the oral microbiota were collected with sterile swabs and then placed in closed tubes with a transport medium. Culture was carried out for media of Gram-negative bacteria. Twenty-two isolates were obtained and subjected to susceptibility tests to twenty-five antimicrobial agents belonging to seven different classes. All tested isolates demonstrated resistance to, at least, one antibiotic, and it was possible to observe multidrug resistance in 11 of the 22 isolates (50%). It should be noted that an isolate showed phenotypic resistance to imipenem, an antibiotic for exclusive use in a hospital environment. All the isolates showed an increasing ability of biofilm formation over time. The obtained results ...
Azathioprine is an immunosuppressive drug for several inflammatory disorders. Due to its clinical... more Azathioprine is an immunosuppressive drug for several inflammatory disorders. Due to its clinical relevance, to explore the solid-state properties for excipient compatibility in the product quality review process is essential. Fourier transform infrared spectroscopy, powder X-ray diffraction and thermal analysis (thermogravimetry/derivative thermogravimetry (TG/DTG), differential thermal analysis (DTA), and differential scanning calorimetry (DSC)) were applied. The compatibility studies evidenced that starch pregelatinized, colloidal silicon dioxide, and talc are fully compatible with azathioprine. However, stearic acid, magnesium stearate, and mannitol are incompatible after heat supply at temperatures easily reached by industrial processing. The nonlinear Vyazovkin isoconversional treatment performed the kinetic study of the thermal degradation. The activation energies were determined to clarify the influence of each excipient on the thermal drug stability, an essential procedure ...
Revista da Sociedade Brasileira de Arborização Urbana, 2019
As árvores que compõem a arborização urbana exercem importantes funções ecológicas e melhoram a h... more As árvores que compõem a arborização urbana exercem importantes funções ecológicas e melhoram a harmonia estética das cidades. Este trabalho teve como objetivos diagnosticar a arborização urbana das áreas de maior trânsito na cidade de São José do Cerrito, SC, detectar possíveis problemas na arborização atual, identificar locais não arborizados e propor soluções para melhorar a arborização existente. Para isso, foram avaliadas as ruas da cidade com maior fluxo de pedestres e de veículos e as praças centrais, onde as árvores foram identificadas, mensuradas e avaliadas quanto a sua ocupação no espaço, presença de injúrias e condições fitossanitárias. Foi encontrada baixa riqueza de espécies, com a predominância de Ligustrum japonicum Thunb., espécie exótica plantada e conduzida de forma inadequada, devida a interferência nas calçadas e na fiação elétrica. A sugestão é que se faça a retirada de grande parte das árvores existentes, mantendo somente as nativas e algumas exóticas com valo...
Gastrointestinal and external parasites from 12 Green-backed Firecrown (Sephanoides sephaniodes) ... more Gastrointestinal and external parasites from 12 Green-backed Firecrown (Sephanoides sephaniodes) were collected between January 2004 and December 2008 from the Biobío Region south-central Chile and Santiago (central Chile). Helminths collected included 1 trematode species (Mosesia sp.) and 1 cestode species (Hymenolepis trinidadensis). The mite Proctophyllodes huitzilopchtlii was the only ectoparasite species found. All these parasites represent new records for Chile and the first parasites reported for this host species.
Increased adipose tissue mass exhibited greater capacity of glucose transformation in lactate, hi... more Increased adipose tissue mass exhibited greater capacity of glucose transformation in lactate, highlighting lactatogenesis as a crucial factor in body size. Classically, lactate produced by isolated adipocytes are expressed per million of cells and were never correlated with their size. Spontaneously hypertensive rats (SHR) have a lower body weight and smaller adipocytes when compared to Wistar-Kyoto. We evaluated basal lactate by epididymal 15weeks-old isolated adipocytes of SHR, Wistar-Kyoto and Wistar. Basal lactate was similar when expressed by one million cells. However, SHR adipocytes were smaller, so we adjusted the results by cell volume and SHR showed higher basal lactate production which was directly endorsed by hyperlactatemia in the presented conditions. Thereby, we suggest a new perspective on lactatogenesis analysis by adipocytes, which could be linked to the receptors density and associate enzymes. Moreover, we showed that the thin and hypertensive rats can be hyperlactemic in fasting conditions.
This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 I... more This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND). Usage and distribution for commercial purposes as well as any distribution of modified material requires written permission.
A magnetic solid-phase microextraction procedure was developed to detect lead in aqueous samples ... more A magnetic solid-phase microextraction procedure was developed to detect lead in aqueous samples by flame atomic absorption spectrometry (FAAS). Fe 3 O 4 nanoparticles were synthesized by coprecipitation and the crystalline phase was confirmed by X-ray diffraction. The point of zero charge (PZC) indicated that the oxide surface was negatively charged at pH levels above 9.4. pH 9.0 is the best for adsorption and the influence of stirring time and adsorbent mass were analyzed and set at 4 min and 3.0 mg, respectively. The best desorption conditions were obtained for 500 µL of 0.5 mol L-1 HNO 3 as eluant. The limit of quantification (LOQ) and relative standard deviation (RSD) were 16.48 µg L-1 and 0.25%, respectively. The linear range was found to be 16.48-500 µg L-1. The accuracy was evaluated by recovery tests in mineral water, micellar water and aqueous makeup remover samples, showing values in the range of 95.94 to 118%.
Epistemological development and attachment theory have been independent frameworks for understand... more Epistemological development and attachment theory have been independent frameworks for understanding psychological development. This study examined the association between epistemological development (using the Measure of Epistemological Reflection) and attachment (using the Adult Attachment Interview) in a sample of 60 pre- and postgraduated college students. Results showed that absolute knowing is rarely present in secure students and that it is most common among preoccupied students. Students with dismissing attachment have restricted ways of knowing, progressing from transitional knowing to independent knowing. For a more comprehensive analysis of these findings selected cases are presented. These results may be relevant for further interdomain research and intervention with college students.
Após um século de Psicologia, entendemos que é necessário integrar no estudo do desenvolvimento h... more Após um século de Psicologia, entendemos que é necessário integrar no estudo do desenvolvimento humano abordagens capazes de nos aproximarem da complexidade dos processos de desenvolvimento e mudança. Na linha de alguns autores, entendemos que é possível aproximar abordagem quantitativas e qualitativas na Psicologia (e.g., Patton, 1990; Mertens, 1998). A nossa proposta é a de que se integrem no plano de investigação diferentes metodologias, efectuando uma aproximação ao problema a partir de diferentes ângulos. Além disso, consideramos que, na avaliação da intervenção em desenvolvimento humano (estudo experimental), as medidas de pré e pós-teste são insuficientes para compreender a complexidade da mudança, assumindo os estudos de follow-up uma importância crucial. Entendemos ainda que é necessário efectuar uma exploração inicial do problema sob investigação e daí a utilidade de um estudo exploratório (estudo transversal de observação), pois permite um esboço inicial do contexto em qu...
The aim of this experiment was to evaluate the effects of cycloxigenase inhibitor drugs, i.e. flu... more The aim of this experiment was to evaluate the effects of cycloxigenase inhibitor drugs, i.e. flunixin meglumine (FM) and parecoxib (P), on the development of bovine embryos cultured in vitro until the blastocyst and hatched blastocyst stages. Immature oocytes were aspirated from slaughterhouse ovaries and morphologically selected for IVM (Monteiro FM et al. 2007 Anim. Reprod. 4, 51–58). Twenty hours after maturation (39°C and 5% CO2 in air), matured oocytes were transferred to fertilization media, inseminated with frozen–thawed semen, and incubated for 10 to 12 h. Presumptive zygotes (PZ) were then transferred to TCM 199 HEPES medium, vortexed to remove cumulus cells and finally to drops of IVC media (SOFaaci plus 5% BFS [Gibco] with 13 mm sodium pyruvate). Each drop of IVC medium had appropriate concentrations of FM (0.14/n = 123; 1.4/n = 122; 14/n = 117; 140/n = 44 or 1400 μg mL–1/n = 44 PZ) or P (0.09/n = 134; 0.9/n = 109; 9/n = 118; 90/n = 113 or 900 μg mL–1/n = 45 PZ), besides...
Revista do Instituto de Medicina Tropical de São Paulo, 2001
More than 70 species of mycobacteria have been defined, and some can cause disease in humans, esp... more More than 70 species of mycobacteria have been defined, and some can cause disease in humans, especially in immunocompromised patients. Species identification in most clinical laboratories is based on phenotypic characteristics and biochemical tests and final results are obtained only after two to four weeks. Quick identification methods, by reducing time for diagnosis, could expedite institution of specific treatment, increasing chances of success. PCR restriction-enzyme analysis (PRA) of the hsp65 gene was used as a rapid method for identification of 103 clinical isolates. Band patterns were interpreted by comparison with published tables and patterns available at an Internet site (http://www.hospvd.ch:8005). Concordant results of PRA and biochemical identification were obtained in 76 out of 83 isolates (91.5%). Results from 20 isolates could not be compared due to inconclusive PRA or biochemical identification. The results of this work showed that PRA could improve identification...
O objetivo deste trabalho foi realizar um estudo morfo-anatômico, bioquímico e fisiológico em sem... more O objetivo deste trabalho foi realizar um estudo morfo-anatômico, bioquímico e fisiológico em sementes de Eremanthus erythropappus (DC.) MacLeish (candeia) durante a germinação. As sementes possuem tegumento de coloração marrom clara e apresentam saliências. O endosperma é bastante reduzido apresentando duas a três camadas de células em torno do embrião. O embrião compõe a maior parte da semente. A condição mais adequada para a germinação das sementes foi fotoperíodo de 14h associado à temperatura alternada de 20-30ºC. Nestas condições, o início da germinação ocorreu após 48h de embebição e a germinação máxima (96%) foi obtida no oitavo dia. As sementes absorveram água segundo um padrão trifásico, sendo a primeira fase rápida e com aumento de massa fresca da semente em 95%. As primeiras modificações morfológicas no tegumento ocorreram após o primeiro dia de embebição. O surgimento de rupturas no tegumento e o crescimento visível da raiz primária coincidiram com a retomada do aumento...
This study was developed in an endemic area of tegumentary leishmaniasis in Minas Gerais, Brazil,... more This study was developed in an endemic area of tegumentary leishmaniasis in Minas Gerais, Brazil, with the objective of analyzing a health education process based on the social representations theory. The educational model was developed in two phases with 34 local residents. In the first phase, social representations of leishmaniasis were identified and analyzed. The second phase was based on the interaction between social representations and scientific knowledge. The results showed that social representations were structured in a central core by the terms "wound" and "mosquito" and in the peripheral system by the terms "mountains", "standing water", and "injection" related respectively to place, transmission, and treatment of the disease. We concluded that tegumentary leishmaniasis is viewed as a wound caused by a mosquito, portrayed by metaphors. The results of the second phase showed that social representations are systems that fa...
A presente pesquisa apresenta um panorama da Psicologia Escolar em escolas públicas e privadas de... more A presente pesquisa apresenta um panorama da Psicologia Escolar em escolas públicas e privadas de Vitória. Foram entrevistados 34 sujeitos, todos de escolas diferentes, sendo quatro psicólogos e 30 outros profissionais de educação. Constatou-se que os poucos psicólogos atuantes não possuíam vínculo empregatício com as escolas e relatavam insatisfação com o trabalho. Os demais profissionais afirmaram que o psicólogo na escola deve trabalhar com os relacionamentos entre alunos, pais e professores. A maioria dos sujeitos considerou o trabalho do psicólogo na escola importante, ainda que, naquelas que não possuíam serviço de psicologia, outros profissionais acabavam por "fazer o papel" do psicólogo.
Nesse trabalho, é discutida a caracterização de filitos na Mina Córrego do Sítio, em Santa Bárbar... more Nesse trabalho, é discutida a caracterização de filitos na Mina Córrego do Sítio, em Santa Bárbara-MG, bem como determinados fatores que influenciam a resistência ao cisalhamento da xistosidade. São apresentados e discutidos resultados de caracterização mineralógica, índices físicos e de ensaios de cisalhamento direto em três áreas dessa Mina. O intemperismo atua na variação de determinadas propriedades, tais como porosidade, índice de vazios, peso específico seco e, também, na resistência mecânica, com queda acentuada de coesão após ensaio de alterabilidade. Por outro lado, a acentuada rugosidade presente na superfície de xistosidade das rochas da Cava Crista influencia no comportamento do maciço rochoso, conferindo-lhe maior resistência ao cisalhamento.
Hyperspectral images in the near infrared range (HSI-NIR) were evaluated as a nondestructive meth... more Hyperspectral images in the near infrared range (HSI-NIR) were evaluated as a nondestructive method to detect fraud in documents. Three different types of typical forgeries were simulated by (a) obliterating text, (b) adding text and (c) approaching the crossing lines problem. The simulated samples were imaged in the range of 928-2524 nm with spectral and spatial resolutions of 6.3 nm and 10 μm, respectively. After data pre-processing, different chemometric techniques were evaluated for each type of forgery. Principal component analysis (PCA) was performed to elucidate the first two types of adulteration, (a) and (b). Moreover, Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) was used in an attempt to improve the results of the type (a) obliteration and type (b) adding text problems. Finally, MCR-ALS and Partial Least Squares-Discriminant Analysis (PLS-DA), employed as a variable selection tool, were used to study the type (c) forgeries, i.e. crossing lines problem....
Myoepithelial cells have been implicated in the regulation of the transition from in situ to inva... more Myoepithelial cells have been implicated in the regulation of the transition from in situ to invasive neoplasia in salivary gland tumors. Considering the importance of the microenvironment of the tumor, the present in vitro study therefore analyzed the morphological and phenotypic changes undergone by benign myoepithelial cells from pleomorphic adenoma (PA) stimulated by tumor-conditioned medium. The benign myoepithelial cells were obtained from PA and were cultured with fibronectin extracellular matrix protein, supplemented with tumor-conditioned medium, which was harvested from breast ductal adenocarcinoma AU-565 and melanoma Hs 852.T cells. The morphological alterations were assessed by immunofluorescence analysis using vimentin antibody. The α-smooth muscle actin (α-SMA) and fibroblast growth factor (FGF)-2 proteins were analyzed by indirect immunofluorescence and quantitative polymerase chain reaction (qPCR). No morphological changes were observed in the myoepithelial cells cul...
O mapeamento morfológico é uma importante ferramenta na interpretação de processos, materiais e f... more O mapeamento morfológico é uma importante ferramenta na interpretação de processos, materiais e formas do relevo regional. Sobretudo em Unidades de Conservação, auxilia no planejamento e gestão ambiental das áreas protegidas. Nesse contexto, o principal objetivo desse trabalho é caracterizar os compartimentos morfológicos do Parque Nacional da Serra do Cipó a partir da medição de parâmetros morfométricos. Os procedimentos metodológicos envolvem a sobreposição de dados cartográficos, a elaboração de perfis topográficos, a identificação de rupturas de declive e, finalmente, medições morfométricas. Os resultados mostram que o Parque Nacional da Serra do Cipó pode ser dividido em onze compartimentos morfológicos na escala de 1:50.000, demonstrando a importância da morfometria na individualização de unidades do relevo. Espera-se que esses resultados e os avanços metodológicos desse trabalho possam colaborar para pesquisas futuras assim como para a gestão de Unidades de Conservação na Ser...
This study aimed to characterize the susceptibility profile to antibiotics and biofilm formation ... more This study aimed to characterize the susceptibility profile to antibiotics and biofilm formation of Gram-negative bacterial isolates obtained from the oral cavity of the black-and-white ruffed lemur (Varecia variegata). From eight individuals from a zoo located in Portugal, samples of the oral microbiota were collected with sterile swabs and then placed in closed tubes with a transport medium. Culture was carried out for media of Gram-negative bacteria. Twenty-two isolates were obtained and subjected to susceptibility tests to twenty-five antimicrobial agents belonging to seven different classes. All tested isolates demonstrated resistance to, at least, one antibiotic, and it was possible to observe multidrug resistance in 11 of the 22 isolates (50%). It should be noted that an isolate showed phenotypic resistance to imipenem, an antibiotic for exclusive use in a hospital environment. All the isolates showed an increasing ability of biofilm formation over time. The obtained results ...
Azathioprine is an immunosuppressive drug for several inflammatory disorders. Due to its clinical... more Azathioprine is an immunosuppressive drug for several inflammatory disorders. Due to its clinical relevance, to explore the solid-state properties for excipient compatibility in the product quality review process is essential. Fourier transform infrared spectroscopy, powder X-ray diffraction and thermal analysis (thermogravimetry/derivative thermogravimetry (TG/DTG), differential thermal analysis (DTA), and differential scanning calorimetry (DSC)) were applied. The compatibility studies evidenced that starch pregelatinized, colloidal silicon dioxide, and talc are fully compatible with azathioprine. However, stearic acid, magnesium stearate, and mannitol are incompatible after heat supply at temperatures easily reached by industrial processing. The nonlinear Vyazovkin isoconversional treatment performed the kinetic study of the thermal degradation. The activation energies were determined to clarify the influence of each excipient on the thermal drug stability, an essential procedure ...
Revista da Sociedade Brasileira de Arborização Urbana, 2019
As árvores que compõem a arborização urbana exercem importantes funções ecológicas e melhoram a h... more As árvores que compõem a arborização urbana exercem importantes funções ecológicas e melhoram a harmonia estética das cidades. Este trabalho teve como objetivos diagnosticar a arborização urbana das áreas de maior trânsito na cidade de São José do Cerrito, SC, detectar possíveis problemas na arborização atual, identificar locais não arborizados e propor soluções para melhorar a arborização existente. Para isso, foram avaliadas as ruas da cidade com maior fluxo de pedestres e de veículos e as praças centrais, onde as árvores foram identificadas, mensuradas e avaliadas quanto a sua ocupação no espaço, presença de injúrias e condições fitossanitárias. Foi encontrada baixa riqueza de espécies, com a predominância de Ligustrum japonicum Thunb., espécie exótica plantada e conduzida de forma inadequada, devida a interferência nas calçadas e na fiação elétrica. A sugestão é que se faça a retirada de grande parte das árvores existentes, mantendo somente as nativas e algumas exóticas com valo...
Gastrointestinal and external parasites from 12 Green-backed Firecrown (Sephanoides sephaniodes) ... more Gastrointestinal and external parasites from 12 Green-backed Firecrown (Sephanoides sephaniodes) were collected between January 2004 and December 2008 from the Biobío Region south-central Chile and Santiago (central Chile). Helminths collected included 1 trematode species (Mosesia sp.) and 1 cestode species (Hymenolepis trinidadensis). The mite Proctophyllodes huitzilopchtlii was the only ectoparasite species found. All these parasites represent new records for Chile and the first parasites reported for this host species.
Increased adipose tissue mass exhibited greater capacity of glucose transformation in lactate, hi... more Increased adipose tissue mass exhibited greater capacity of glucose transformation in lactate, highlighting lactatogenesis as a crucial factor in body size. Classically, lactate produced by isolated adipocytes are expressed per million of cells and were never correlated with their size. Spontaneously hypertensive rats (SHR) have a lower body weight and smaller adipocytes when compared to Wistar-Kyoto. We evaluated basal lactate by epididymal 15weeks-old isolated adipocytes of SHR, Wistar-Kyoto and Wistar. Basal lactate was similar when expressed by one million cells. However, SHR adipocytes were smaller, so we adjusted the results by cell volume and SHR showed higher basal lactate production which was directly endorsed by hyperlactatemia in the presented conditions. Thereby, we suggest a new perspective on lactatogenesis analysis by adipocytes, which could be linked to the receptors density and associate enzymes. Moreover, we showed that the thin and hypertensive rats can be hyperlactemic in fasting conditions.
This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 I... more This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND). Usage and distribution for commercial purposes as well as any distribution of modified material requires written permission.
A magnetic solid-phase microextraction procedure was developed to detect lead in aqueous samples ... more A magnetic solid-phase microextraction procedure was developed to detect lead in aqueous samples by flame atomic absorption spectrometry (FAAS). Fe 3 O 4 nanoparticles were synthesized by coprecipitation and the crystalline phase was confirmed by X-ray diffraction. The point of zero charge (PZC) indicated that the oxide surface was negatively charged at pH levels above 9.4. pH 9.0 is the best for adsorption and the influence of stirring time and adsorbent mass were analyzed and set at 4 min and 3.0 mg, respectively. The best desorption conditions were obtained for 500 µL of 0.5 mol L-1 HNO 3 as eluant. The limit of quantification (LOQ) and relative standard deviation (RSD) were 16.48 µg L-1 and 0.25%, respectively. The linear range was found to be 16.48-500 µg L-1. The accuracy was evaluated by recovery tests in mineral water, micellar water and aqueous makeup remover samples, showing values in the range of 95.94 to 118%.
Epistemological development and attachment theory have been independent frameworks for understand... more Epistemological development and attachment theory have been independent frameworks for understanding psychological development. This study examined the association between epistemological development (using the Measure of Epistemological Reflection) and attachment (using the Adult Attachment Interview) in a sample of 60 pre- and postgraduated college students. Results showed that absolute knowing is rarely present in secure students and that it is most common among preoccupied students. Students with dismissing attachment have restricted ways of knowing, progressing from transitional knowing to independent knowing. For a more comprehensive analysis of these findings selected cases are presented. These results may be relevant for further interdomain research and intervention with college students.
Após um século de Psicologia, entendemos que é necessário integrar no estudo do desenvolvimento h... more Após um século de Psicologia, entendemos que é necessário integrar no estudo do desenvolvimento humano abordagens capazes de nos aproximarem da complexidade dos processos de desenvolvimento e mudança. Na linha de alguns autores, entendemos que é possível aproximar abordagem quantitativas e qualitativas na Psicologia (e.g., Patton, 1990; Mertens, 1998). A nossa proposta é a de que se integrem no plano de investigação diferentes metodologias, efectuando uma aproximação ao problema a partir de diferentes ângulos. Além disso, consideramos que, na avaliação da intervenção em desenvolvimento humano (estudo experimental), as medidas de pré e pós-teste são insuficientes para compreender a complexidade da mudança, assumindo os estudos de follow-up uma importância crucial. Entendemos ainda que é necessário efectuar uma exploração inicial do problema sob investigação e daí a utilidade de um estudo exploratório (estudo transversal de observação), pois permite um esboço inicial do contexto em qu...
The aim of this experiment was to evaluate the effects of cycloxigenase inhibitor drugs, i.e. flu... more The aim of this experiment was to evaluate the effects of cycloxigenase inhibitor drugs, i.e. flunixin meglumine (FM) and parecoxib (P), on the development of bovine embryos cultured in vitro until the blastocyst and hatched blastocyst stages. Immature oocytes were aspirated from slaughterhouse ovaries and morphologically selected for IVM (Monteiro FM et al. 2007 Anim. Reprod. 4, 51–58). Twenty hours after maturation (39°C and 5% CO2 in air), matured oocytes were transferred to fertilization media, inseminated with frozen–thawed semen, and incubated for 10 to 12 h. Presumptive zygotes (PZ) were then transferred to TCM 199 HEPES medium, vortexed to remove cumulus cells and finally to drops of IVC media (SOFaaci plus 5% BFS [Gibco] with 13 mm sodium pyruvate). Each drop of IVC medium had appropriate concentrations of FM (0.14/n = 123; 1.4/n = 122; 14/n = 117; 140/n = 44 or 1400 μg mL–1/n = 44 PZ) or P (0.09/n = 134; 0.9/n = 109; 9/n = 118; 90/n = 113 or 900 μg mL–1/n = 45 PZ), besides...
Revista do Instituto de Medicina Tropical de São Paulo, 2001
More than 70 species of mycobacteria have been defined, and some can cause disease in humans, esp... more More than 70 species of mycobacteria have been defined, and some can cause disease in humans, especially in immunocompromised patients. Species identification in most clinical laboratories is based on phenotypic characteristics and biochemical tests and final results are obtained only after two to four weeks. Quick identification methods, by reducing time for diagnosis, could expedite institution of specific treatment, increasing chances of success. PCR restriction-enzyme analysis (PRA) of the hsp65 gene was used as a rapid method for identification of 103 clinical isolates. Band patterns were interpreted by comparison with published tables and patterns available at an Internet site (http://www.hospvd.ch:8005). Concordant results of PRA and biochemical identification were obtained in 76 out of 83 isolates (91.5%). Results from 20 isolates could not be compared due to inconclusive PRA or biochemical identification. The results of this work showed that PRA could improve identification...
O objetivo deste trabalho foi realizar um estudo morfo-anatômico, bioquímico e fisiológico em sem... more O objetivo deste trabalho foi realizar um estudo morfo-anatômico, bioquímico e fisiológico em sementes de Eremanthus erythropappus (DC.) MacLeish (candeia) durante a germinação. As sementes possuem tegumento de coloração marrom clara e apresentam saliências. O endosperma é bastante reduzido apresentando duas a três camadas de células em torno do embrião. O embrião compõe a maior parte da semente. A condição mais adequada para a germinação das sementes foi fotoperíodo de 14h associado à temperatura alternada de 20-30ºC. Nestas condições, o início da germinação ocorreu após 48h de embebição e a germinação máxima (96%) foi obtida no oitavo dia. As sementes absorveram água segundo um padrão trifásico, sendo a primeira fase rápida e com aumento de massa fresca da semente em 95%. As primeiras modificações morfológicas no tegumento ocorreram após o primeiro dia de embebição. O surgimento de rupturas no tegumento e o crescimento visível da raiz primária coincidiram com a retomada do aumento...
This study was developed in an endemic area of tegumentary leishmaniasis in Minas Gerais, Brazil,... more This study was developed in an endemic area of tegumentary leishmaniasis in Minas Gerais, Brazil, with the objective of analyzing a health education process based on the social representations theory. The educational model was developed in two phases with 34 local residents. In the first phase, social representations of leishmaniasis were identified and analyzed. The second phase was based on the interaction between social representations and scientific knowledge. The results showed that social representations were structured in a central core by the terms "wound" and "mosquito" and in the peripheral system by the terms "mountains", "standing water", and "injection" related respectively to place, transmission, and treatment of the disease. We concluded that tegumentary leishmaniasis is viewed as a wound caused by a mosquito, portrayed by metaphors. The results of the second phase showed that social representations are systems that fa...
A presente pesquisa apresenta um panorama da Psicologia Escolar em escolas públicas e privadas de... more A presente pesquisa apresenta um panorama da Psicologia Escolar em escolas públicas e privadas de Vitória. Foram entrevistados 34 sujeitos, todos de escolas diferentes, sendo quatro psicólogos e 30 outros profissionais de educação. Constatou-se que os poucos psicólogos atuantes não possuíam vínculo empregatício com as escolas e relatavam insatisfação com o trabalho. Os demais profissionais afirmaram que o psicólogo na escola deve trabalhar com os relacionamentos entre alunos, pais e professores. A maioria dos sujeitos considerou o trabalho do psicólogo na escola importante, ainda que, naquelas que não possuíam serviço de psicologia, outros profissionais acabavam por "fazer o papel" do psicólogo.
Nesse trabalho, é discutida a caracterização de filitos na Mina Córrego do Sítio, em Santa Bárbar... more Nesse trabalho, é discutida a caracterização de filitos na Mina Córrego do Sítio, em Santa Bárbara-MG, bem como determinados fatores que influenciam a resistência ao cisalhamento da xistosidade. São apresentados e discutidos resultados de caracterização mineralógica, índices físicos e de ensaios de cisalhamento direto em três áreas dessa Mina. O intemperismo atua na variação de determinadas propriedades, tais como porosidade, índice de vazios, peso específico seco e, também, na resistência mecânica, com queda acentuada de coesão após ensaio de alterabilidade. Por outro lado, a acentuada rugosidade presente na superfície de xistosidade das rochas da Cava Crista influencia no comportamento do maciço rochoso, conferindo-lhe maior resistência ao cisalhamento.
Hyperspectral images in the near infrared range (HSI-NIR) were evaluated as a nondestructive meth... more Hyperspectral images in the near infrared range (HSI-NIR) were evaluated as a nondestructive method to detect fraud in documents. Three different types of typical forgeries were simulated by (a) obliterating text, (b) adding text and (c) approaching the crossing lines problem. The simulated samples were imaged in the range of 928-2524 nm with spectral and spatial resolutions of 6.3 nm and 10 μm, respectively. After data pre-processing, different chemometric techniques were evaluated for each type of forgery. Principal component analysis (PCA) was performed to elucidate the first two types of adulteration, (a) and (b). Moreover, Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) was used in an attempt to improve the results of the type (a) obliteration and type (b) adding text problems. Finally, MCR-ALS and Partial Least Squares-Discriminant Analysis (PLS-DA), employed as a variable selection tool, were used to study the type (c) forgeries, i.e. crossing lines problem....
Myoepithelial cells have been implicated in the regulation of the transition from in situ to inva... more Myoepithelial cells have been implicated in the regulation of the transition from in situ to invasive neoplasia in salivary gland tumors. Considering the importance of the microenvironment of the tumor, the present in vitro study therefore analyzed the morphological and phenotypic changes undergone by benign myoepithelial cells from pleomorphic adenoma (PA) stimulated by tumor-conditioned medium. The benign myoepithelial cells were obtained from PA and were cultured with fibronectin extracellular matrix protein, supplemented with tumor-conditioned medium, which was harvested from breast ductal adenocarcinoma AU-565 and melanoma Hs 852.T cells. The morphological alterations were assessed by immunofluorescence analysis using vimentin antibody. The α-smooth muscle actin (α-SMA) and fibroblast growth factor (FGF)-2 proteins were analyzed by indirect immunofluorescence and quantitative polymerase chain reaction (qPCR). No morphological changes were observed in the myoepithelial cells cul...
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