La presente invention concerne un procede de fabrication d'adenovirus comprenant une fibre et... more La presente invention concerne un procede de fabrication d'adenovirus comprenant une fibre et un hexon du sous-groupe B (tels que Ad11, en particulier Ad11p egalement connu sous le nom de souche Slobitski), la region E4 etant complete ou totalement absente, ledit procede comprenant les etapes consistant a : a. mettre en culture des cellules de mammifere infectees par l'adenovirus en presence d'un support approprie pour supporter les cellules de sorte que le virus se replique, les cellules etant capables de supporter la replication virale, et b. a la fin de la periode de culture, isoler le virus provenant de l'etape a) a partir du milieu par a) filtration, l'isolement du virus n'etant pas consecutif a une etape de lyse cellulaire. L'invention concerne egalement des formulations et des virus obtenus a partir du procede.
Additional file 8. 3FR BiTE treatment triggers wide-ranging increases in immunomodulatory cytokin... more Additional file 8. 3FR BiTE treatment triggers wide-ranging increases in immunomodulatory cytokines and chemokines. Cell-free supernatants from two ascites samples treated (or not) for five days with 3Ctrl or 3FR BiTE were assessed using a 13-plex immunoassay. Fold-increases in cytokine/chemokine levels were calculated relative to "Mock"-treated samples. Data show mean ± SD of biological triplicates.
Immunoblotting of FLAG-tagged MAV1 ORF transgenes encoded in EnAd. NMuMG-CD46 cells were infected... more Immunoblotting of FLAG-tagged MAV1 ORF transgenes encoded in EnAd. NMuMG-CD46 cells were infected with EnAd encoding different MAV1 ORFs as CMV-driven FLAG-tagged transgenes. A. Three days post-infection, cells were lysed and probed for the presence of the FLAG tag using a horseradish peroxidase-conjugated mouse anti-DYKDDDDK antibody. Red asterisks above bands denote proteins of approximately the predicted size for each transgene. Blots were probed with horseradish peroxidase-conjugated mouse anti-β-actin as a loading control. B. Predicted protein sizes for each ORF. (PPTX 1369 kb)
TPS3112Background: EnAd is a tumor-selective chimeric Ad11/Ad3 group B oncolytic adenovirus devel... more TPS3112Background: EnAd is a tumor-selective chimeric Ad11/Ad3 group B oncolytic adenovirus developed using a process of directed evolution. Phase I clinical studies have identified a well-tolerate...
The present invention relates to the provision of novel immunogens comprising an antigenic IgE pe... more The present invention relates to the provision of novel immunogens comprising an antigenic IgE peptide preferably linked to an immunogenic carrier for the prevention, treatment or alleviation of IgE-mediated disorders. The invention further relates to methods for production of these medicaments, immunogenic compositions and pharmaceutical compositions thereof and their use in medicine.
Mouse monoclonal antibodies against recombinant human fibroblast procollagenase and prostromelysi... more Mouse monoclonal antibodies against recombinant human fibroblast procollagenase and prostromelysin have been generated and characterized. The epitope-containing domains for the antibodies have been assigned based on their immunoreactivities against recombinant proenzymes, ...
Peripheral T cell tolerance is critical in the regulation of immune responses to self antigens, a... more Peripheral T cell tolerance is critical in the regulation of immune responses to self antigens, and has implications in the control of autoimmunity, allergic responses and transplant rejection. Here we discuss recent and unpublished data demonstrating that ligation of the cell surface receptor Notch on T cells inhibits immune responses and results in the generation of a regulatory T cell population. Using animal models, we show that prior exposure to antigen can inhibit the response to challenge in an antigen-specific fashion. This inhibition is accompanied by the generation of cells with apparent regulatory capacity and we further show that exposure of T cells to Notch and T cell receptor signals together enhances interleukin 10 production, at least in cell culture systems. We discuss how Notch signalling may integrate with other signals to induce tolerance.
Affinity matured murine monoclonal antibody producing cell lines can now be rapidly generated usi... more Affinity matured murine monoclonal antibody producing cell lines can now be rapidly generated using a novel repetitive, multiple site immunization strategy designated RIMMS. RIMMS capitalizes on rapid hypermuta¬ tion and affinity maturation events which occur in cell populations localized within secondary lymphatic tissue early in response to antigenic challenges. A murine myeloma cell line, P3XBcl-2-13, stably transfected with Bcl-2, enhances the outgrowth of hybridomas following somatic fusion with immune lymphocytes iso¬ lated from pooled peripheral lymph nodes (PLN) 8-14 days after the initial immunization. Immunizations, so¬ matic fusion, screening and isolation of affinity matured IgG secreting monoclonal antibody cell lines occur within a one month time period. By using RIMMS, we have been able to expedite the isolation of affinity ma¬ tured monoclonal antibodies to numerous antigens, including a drug hapten.
La presente invention concerne un procede de fabrication d'adenovirus comprenant une fibre et... more La presente invention concerne un procede de fabrication d'adenovirus comprenant une fibre et un hexon du sous-groupe B (tels que Ad11, en particulier Ad11p egalement connu sous le nom de souche Slobitski), la region E4 etant complete ou totalement absente, ledit procede comprenant les etapes consistant a : a. mettre en culture des cellules de mammifere infectees par l'adenovirus en presence d'un support approprie pour supporter les cellules de sorte que le virus se replique, les cellules etant capables de supporter la replication virale, et b. a la fin de la periode de culture, isoler le virus provenant de l'etape a) a partir du milieu par a) filtration, l'isolement du virus n'etant pas consecutif a une etape de lyse cellulaire. L'invention concerne egalement des formulations et des virus obtenus a partir du procede.
Additional file 8. 3FR BiTE treatment triggers wide-ranging increases in immunomodulatory cytokin... more Additional file 8. 3FR BiTE treatment triggers wide-ranging increases in immunomodulatory cytokines and chemokines. Cell-free supernatants from two ascites samples treated (or not) for five days with 3Ctrl or 3FR BiTE were assessed using a 13-plex immunoassay. Fold-increases in cytokine/chemokine levels were calculated relative to "Mock"-treated samples. Data show mean ± SD of biological triplicates.
Immunoblotting of FLAG-tagged MAV1 ORF transgenes encoded in EnAd. NMuMG-CD46 cells were infected... more Immunoblotting of FLAG-tagged MAV1 ORF transgenes encoded in EnAd. NMuMG-CD46 cells were infected with EnAd encoding different MAV1 ORFs as CMV-driven FLAG-tagged transgenes. A. Three days post-infection, cells were lysed and probed for the presence of the FLAG tag using a horseradish peroxidase-conjugated mouse anti-DYKDDDDK antibody. Red asterisks above bands denote proteins of approximately the predicted size for each transgene. Blots were probed with horseradish peroxidase-conjugated mouse anti-β-actin as a loading control. B. Predicted protein sizes for each ORF. (PPTX 1369 kb)
TPS3112Background: EnAd is a tumor-selective chimeric Ad11/Ad3 group B oncolytic adenovirus devel... more TPS3112Background: EnAd is a tumor-selective chimeric Ad11/Ad3 group B oncolytic adenovirus developed using a process of directed evolution. Phase I clinical studies have identified a well-tolerate...
The present invention relates to the provision of novel immunogens comprising an antigenic IgE pe... more The present invention relates to the provision of novel immunogens comprising an antigenic IgE peptide preferably linked to an immunogenic carrier for the prevention, treatment or alleviation of IgE-mediated disorders. The invention further relates to methods for production of these medicaments, immunogenic compositions and pharmaceutical compositions thereof and their use in medicine.
Mouse monoclonal antibodies against recombinant human fibroblast procollagenase and prostromelysi... more Mouse monoclonal antibodies against recombinant human fibroblast procollagenase and prostromelysin have been generated and characterized. The epitope-containing domains for the antibodies have been assigned based on their immunoreactivities against recombinant proenzymes, ...
Peripheral T cell tolerance is critical in the regulation of immune responses to self antigens, a... more Peripheral T cell tolerance is critical in the regulation of immune responses to self antigens, and has implications in the control of autoimmunity, allergic responses and transplant rejection. Here we discuss recent and unpublished data demonstrating that ligation of the cell surface receptor Notch on T cells inhibits immune responses and results in the generation of a regulatory T cell population. Using animal models, we show that prior exposure to antigen can inhibit the response to challenge in an antigen-specific fashion. This inhibition is accompanied by the generation of cells with apparent regulatory capacity and we further show that exposure of T cells to Notch and T cell receptor signals together enhances interleukin 10 production, at least in cell culture systems. We discuss how Notch signalling may integrate with other signals to induce tolerance.
Affinity matured murine monoclonal antibody producing cell lines can now be rapidly generated usi... more Affinity matured murine monoclonal antibody producing cell lines can now be rapidly generated using a novel repetitive, multiple site immunization strategy designated RIMMS. RIMMS capitalizes on rapid hypermuta¬ tion and affinity maturation events which occur in cell populations localized within secondary lymphatic tissue early in response to antigenic challenges. A murine myeloma cell line, P3XBcl-2-13, stably transfected with Bcl-2, enhances the outgrowth of hybridomas following somatic fusion with immune lymphocytes iso¬ lated from pooled peripheral lymph nodes (PLN) 8-14 days after the initial immunization. Immunizations, so¬ matic fusion, screening and isolation of affinity matured IgG secreting monoclonal antibody cell lines occur within a one month time period. By using RIMMS, we have been able to expedite the isolation of affinity ma¬ tured monoclonal antibodies to numerous antigens, including a drug hapten.
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Papers by Brian Champion