Papers by Boubacar COULIBALY
Malaria Journal
Background Azithromycin has recently been shown to reduce all-cause childhood mortality in sub-Sa... more Background Azithromycin has recently been shown to reduce all-cause childhood mortality in sub-Saharan Africa. One potential mechanism of this effect is via the anti-malarial effect of azithromycin, which may help treat or prevent malaria infection. This study evaluated short- and longer-term effects of azithromycin on malaria outcomes in children. Methods Children aged 8 days to 59 months were randomized in a 1:1 fashion to a single oral dose of azithromycin (20 mg/kg) or matching placebo. Children were evaluated for malaria via thin and thick smear and rapid diagnostic test (for those with tympanic temperature ≥ 37.5 °C) at baseline and 14 days and 6 months after treatment. Malaria outcomes in children receiving azithromycin versus placebo were compared at each follow-up timepoint separately. Results Of 450 children enrolled, 230 were randomized to azithromycin and 220 to placebo. Children were a median of 26 months and 51% were female, and 17% were positive for malaria parasitaem...
Acta Tropica, 2021
Malaria vector control in Mali relies heavily on the use of long-lasting insecticidal nets (LLINs... more Malaria vector control in Mali relies heavily on the use of long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) in selected districts. As part of strengthening vector control strategies in Koulikoro district, the National Malaria Control Programme (NMCP) through the support from the US President's Malaria Initiative (PMI) has strategically driven the implementation of IRS, with the LLINs coverage also rising from 93.3% and 98.2%. Due to the increased reports of vector resistance to both pyrethroid and carbamates, there was a campaign for the use of pirimiphos-methyl, an organophosphate at Koulikoro between 2015 and 2016. In this study, the effect of IRS on malaria transmission was assessed, by comparing some key entomological indices between Koulikoro, where IRS was implemented and its neighboring district, Banamba that has never received IRS as vector control intervention. The study was conducted in two villages of each district (Koulikoro and Banamba). Pyrethrum spray catches and entry window trapping were used to collect mosquitoes on a monthly basis. WHO tube tests were
Additional file 2: Supplemental Table S1. Baseline demographic and anthropometric characteristics... more Additional file 2: Supplemental Table S1. Baseline demographic and anthropometric characteristics among children lost and not lost to follow-up at 14 days and 6 months. (PDF 2577 kb)
Additional file 1. Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) 20... more Additional file 1. Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) 2013 Checklist: recommended items to address in a clinical trial protocol and related documents*.
Manhattan plot for colony Fd03. Results are given for the two replicates individually and the val... more Manhattan plot for colony Fd03. Results are given for the two replicates individually and the values combined by Fisher's method. All genotyped loci were tested for association with two phenotypes by logistic regression using PLINK. Infection prevalence (blue) was defined as having more than one oocyst in the dissected midgut, infection intensity (red, measured only for mosquitoes carrying ≥1 oocyst) as having >5 oocysts. Dashed line represents a 0.01 p-value (i.e., 1/p = 100). Genes in the regions are shown below; Toll11 and Toll10 are the two rightmost genes on the positive strand. The combined plot represents values from reps 1 and 2 combined by the method of Fisher [47]. (PDF 127 kb)
Fd03 Infection Prevalence Association and Table S2. Fd03 Infection Intensity Association (XLSX 15... more Fd03 Infection Prevalence Association and Table S2. Fd03 Infection Intensity Association (XLSX 15Â kb)
All predicted genes within the mapped locus, Pfin 7. (XLSX 13Â kb)
Genome wide total heterozygosity and relative heterozygosity for founder colony Fd03. Plots depic... more Genome wide total heterozygosity and relative heterozygosity for founder colony Fd03. Plots depict heterozygosity measures for colony Fd03 across all chromosomes. Total pooled heterozygosity (Hp) was calculated in a sliding 10 kb window along the chromosome within the Fd03 colony. Dots indicate minimum and maximum values for a 1 Mb window, the black line indicates the average heterozygosity and the gray shading represents the standard deviation of total pooled heterozygosity across a 1 Mb window. Relative diversity (HpR) per 1 Mb window, calculated as the proportion of heterozygosity in a given pool relative to total heterozygosity within the source Fd03 colony. Color of point indicates per window elevated heterozygosity (blue), or reduced heterozygosity (red), plotted as log base 10 of the relative diversity. Phenotype pool identity is indicated in the lower left of each panel (high, low, zero oocysts). A relative heterozygosity value of 1 indicates the same heterozygosity levels i...
Concordance of allele frequencies as called from pooled sequence and Sequenom individual genotypi... more Concordance of allele frequencies as called from pooled sequence and Sequenom individual genotyping. Concordance was assessed with data from three Sequenom plexes spread across all 3 candidate loci, 3.1, 9.1, and 9.2. SNPs were chosen independently of the phenotype group; that is, they were chosen on the basis of differing allele frequencies in either of the founder pools, and not for high allele differences between phenotype pools. Allele frequencies for Sequenom data were calculated from genotype calls and allele frequencies from pool sequence were determined by relative read depth. Dot color indicates the founder colony and phenotype group as indicated in the legend. (PDF 113Â kb)
This article cites 49 articles, 25 of which can be accessed free
An improved method for physician-certified verbal autopsy reduces the rate of discrepancy:
Methylene blue for malaria in Africa: results from a dose-finding study in combination with chlor... more Methylene blue for malaria in Africa: results from a dose-finding study in combination with chloroquine
Abstract: The presence of antigen-specific cellular immune responses may be an indicator of long-... more Abstract: The presence of antigen-specific cellular immune responses may be an indicator of long-term asymptomatic HIV-1-disease. The detection of cellular immune responses to infection with different subtypes of HIV-1 may be hampered by genetic differences of immunodominant antigens such as the capsid protein CAp24. In Nouna, Burkina Faso, HIV-1 circulating recombinant forms CRF02_AG and CRF06_cpx are the 2 major strains detectable in HIV-1-infected individuals, while subtype B strains prevail in Europe and North America. Amino acid sequences of CAp24 were assessed in blood samples from 10 HIV-1-infected patients in Nouna, Burkina Faso. Production of interferon-gamma (IFN-�) in peripheral blood CD4 + lymphocytes in response to recombinant HIV-1 proteins derived from clade B (including CAp24NL4-3) was measured using a modified flow-cytometry-based whole blood short term activation assay (FASTimmune, BDBiosciences). IFN- � production following stimulation with a whole length CAp24 pr...
The Journal of Infection in Developing Countries, 2020
Introduction: Extended-Spectrum Beta-Lactamases (ESBL) are a common mechanism of bacterial resist... more Introduction: Extended-Spectrum Beta-Lactamases (ESBL) are a common mechanism of bacterial resistance in Enterobacteriaceae. The purpose of this study is to characterize the ESBL genes produced by community-acquired uropathogenic Escherichia coli strains in the Nouna District, in the West-African country, Burkina Faso. Methodology: Samples were collected from non-hospitalized patients who came for consultation at the CMA (Centre Médical avec Antenne chirurgicale) in Nouna and were sent to the laboratory for a urine culture test. The detection of ESBL production by the bacteria was carried out with the double-disc synergy test and the extraction of the ESBL genes with the heat shock method. Molecular characterization of ESBL genes was performed with three sequential multiplex polymerase chain reaction (PCR) assays. Results: One hundred and eighty-two (182) bacteriological cultures were analyzed and 29 E. coli isolated, between 01/07/2017 and 01/07/2018. The ESBL phenotype was found i...
Frontiers in Microbiology, 2020
Background: Antimicrobial resistance (AMR) thwarts the curative power of drugs and is a present-t... more Background: Antimicrobial resistance (AMR) thwarts the curative power of drugs and is a present-time global problem. We present data on antimicrobial susceptibility and resistance determinants of bacteria the WHO has highlighted as being key antimicrobial resistance concerns in Africa, to strengthen knowledge of AMR patterns in the region. Methods: Blood, stool, and urine specimens of febrile patients, aged between ≥ 30 days and ≤ 15 years and hospitalized in Burkina Faso, Gabon, Ghana, and Tanzania were cultured from November 2013 to March 2017 (Patients > 15 years were included in Tanzania). Antimicrobial susceptibility testing was performed for all Enterobacterales and Staphylococcus aureus isolates using disk diffusion method. Extended-spectrum beta-lactamase (ESBL) production was confirmed by double-disk diffusion test and the detection of bla CTX−M , bla TEM and bla SHV. Multilocus sequence typing was conducted for ESBL-producing Escherichia coli and Klebsiella pneumoniae, ciprofloxacin-resistant Salmonella enterica and S. aureus. Ciprofloxacin-resistant
Citation correct de ce document / Correct citation of this document: Noussourou, M.et al. (2020).... more Citation correct de ce document / Correct citation of this document: Noussourou, M.et al. (2020). Caracterisation de l’entomofaune dans le systeme de decrue du cercle de Yelimane en zone sahelienne au Mali. In: N'Diaye, I. et al. (eds.) Adaptation de l'Agriculture et de l'Elevage au Changement Climatique au Mali: Resultats et lecons apprises au Sahel. Bamako, Mali: Institut d'Economie Rurale. pp. 147-158.
Numerous studies have shown the influence of some bacteria colonizing the breeding sites on the d... more Numerous studies have shown the influence of some bacteria colonizing the breeding sites on the development of the parasite in Anopheles malaria vectors. The aim of this study was to evaluate the impact of the breeding sites of Plasmodium falciparum on the gametocytes infectivity of Anopheles gambiae sl using the membrane feeding method. We carried out an experimental infestation study from September 2010 to January 2011 in a village located in the southern savanna of Mali. Cross sectional surveys were conducted to collect larvae and to select gametocyte carriers. Female offspring of L3 and L4 stage larvae were used for the experimental infestation. The gametocyte carriers were children aged within 4 to 12 years. Bacteria identified in breeding water of larvae and adult mosquitoes were: Escherichia coli, Salmonella spp, Klebsiella oxytoca, Pseudomonas spp, Staphylococcus spp, Shigella sp. The oocyst load of infected mosquitoes did not significantly vary according to the cottages (F ...
Citation correct de ce document / Correct citation of this document: Traore, K. et al. (2020). Ca... more Citation correct de ce document / Correct citation of this document: Traore, K. et al. (2020). Caracteristiques physico-chimiques des horizons de surface des sols de decrue a Gory, Dougoubara et Yaguine, cercle de Yelimane. In: N'Diaye, I. et al. (eds.) Adaptation de l'Agriculture et de l'Elevage au Changement Climatique au Mali: Resultats et lecons apprises au Sahel. Bamako, Mali: Institut d'Economie Rurale. pp. 131-145
The American Journal of Tropical Medicine and Hygiene, 2021
ABSTRACTZoonotic transmission is likely a pathway for antibiotic resistance. Data from a randomiz... more ABSTRACTZoonotic transmission is likely a pathway for antibiotic resistance. Data from a randomized trial of pediatric antibiotic administration were secondarily evaluated to determine if poultry ownership was significantly associated with the presence of gut genetic antibiotic resistance determinants among 118 children in Burkina Faso. Antimicrobial resistance (AMR) determinants were classified using DNA sequencing. We measured the relationship between genetic resistance determinants and chicken ownership using a logistic regression model adjusted for confounding variables. Children in households reporting poultry ownership had four times the odds of tetracycline resistance determinants in the gut compared with those without household poultry (odds ratio [OR]: 4.08, 95% CI: 1.08–15.44, P = 0.04). There was no statistically significant difference found for other antibiotic classes. Understanding the origins of antibiotic resistance may help spur the development of interventions to c...
Uploads
Papers by Boubacar COULIBALY