... ANANYA BHAR, CHHOTAN KUMAR GHOSH, S BANDYOPADHYAY, SAMAR SARKAR, AMALENDU CHAKRABART, GAUTAM ... more ... ANANYA BHAR, CHHOTAN KUMAR GHOSH, S BANDYOPADHYAY, SAMAR SARKAR, AMALENDU CHAKRABART, GAUTAM DATTA, BISWAJIT AUDDY, PRATIP K DEBNATH. Full Text: PDF. Refbacks. There are currently no refbacks. Bookmark and Share.
India possessed long coastal areas where venomous catfish are abundant. But almost none of these ... more India possessed long coastal areas where venomous catfish are abundant. But almost none of these venomous fish species has been touched for detail investigation regarding their venoms and the active components present in the venom. Plotosus Canius is one of such major venomous catfish species that is present in the estuaries of eastern India. Sting of these fish produces intense pain, swelling and gangrene of the affected areas. On experimental animals the venom produced hypertension and respiratory failure, cardiac arrest, neuromuscular blockage and the LD50 was found to be 3.9 mg/kg (ip) in male albino mice (Auddy et al, 1993). An attempt was made to isolate and purify the lethal factor from the crude venom and its probable involvement in pathophysiology.
The venom of the common Indian catfish P. canius Hamilton (locally called 'Kanmagur') was examine... more The venom of the common Indian catfish P. canius Hamilton (locally called 'Kanmagur') was examined for its pharmacodynamic activity. The LD50 of the venom in mice was found to be 3.9 mg/kg (ip). At lower doses, the venom produced a positive inotropic effect on toad and rabbit hearts, while at higher doses it produced cardiac arrest. In the isolated guinea pig auricle, the venom increased the rate and amplitude of contraction. The venom increased rat blood pressure--an action antagonised by alpha-adrenergic blocker (phenoxybenzamine). It reduced the rate and amplitude of rat and guinea pig respiration leading to respiratory arrest and death. The venom did not alter the cutaneous capillary permeability of guinea pig but produced vasoconstrictor effect on rat hindquarter perfusion. It induced contractions in several smooth muscle preparations viz., ileum and colon of guinea pig, fundus, uterus and ileum of rat. On isolated guinea pig ileum, the venom produced contraction which was not antagonised by atropine and mepyramine, but was partially antagonised by methysergide associated with a residual contraction which was abolished by SC 19220, a prostaglandin receptor blocker. The venom produced irreversible blockade of electrically induced twitch response on isolated rat phrenic nerve diaphragm and chick biventer cervicis preparation. Haemolysis was not produced by the venom on mice, guinea pig and human RBC (washed).
Aim: To study wound healing activity of the human placental extract (HPE) in rats. Methods: Full ... more Aim: To study wound healing activity of the human placental extract (HPE) in rats. Methods: Full thickness wounds were inflicted on depilated dorsum of Charles foster rats with 8 mm Acu-punch biopsy. The HPE was applied both at topical and im routes (2.5 mL/kg). Effects were compared on the basis of physical criteria, biochemical criteria, and histopathological study with respect to untreated control, vehicle control (1.5 % benzyl alcohol), and framycetin topical treated groups. Results: Significant lowering of wound size (P<0.05), wound index (P<0.05), and number of days required for complete healing (P<0.01); significant gain in tensile strength (P<0.01); appreciable increase of tissue DNA, total protein, and collagenesis were observed in HPE treated group. Conclusion: Human placental extract systematically helps collagenesis leading to potent healing of wounds.
Cigarette smoke contains oxidants which generate long lasting free radicals that cause oxidative ... more Cigarette smoke contains oxidants which generate long lasting free radicals that cause oxidative stress in active and passive smokers leading to pathological conditions. Antioxidant nutrients are reported to prevent the oxidative damage induced by smoking. In the present study, we have evaluated the antioxidant role of Phyllanthus emblica (PE) fruit extract on cigarette smoke-induced oxidative stress in rats. Adult male albino rats were exposed to cigarette smoke and administered daily with PE fruit extract (Gr.A) or vitamin supplements (Gr.D). Another group of animals were exposed to only cigarette smoke (Gr.B) and control animals were subjected to sham session (Gr.C). Group A, B and D animals were kept in a smoke chamber for 30 min, twice a day for 4 weeks. Hemoglobin (Hb) % and malondialdehyde (MDA) content of RBC, lung and liver homogenate; superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and glutathione-S-transferases (GST) of lung and liver homogenate of different treatment groups were estimated. Administration of PE fruit extract showed increase in the Hb level (+ 23.4 %) of Gr.A animals with concomitant decrease in MDA levels of RBC, lung and liver homogenate (48-54%, p<0.001) when compared to those of group B animals. The status of improvement of SOD, CAT, GSH and GST of lung and liver homogenate in PE fruit extract-administered group (Gr.A) ranged from 30-80% over the only smoke exposed group (Gr.B). PE fruit extract administered group showed better antioxidant profiles than that of the multivitamin supplemented group. The results of the present study suggest that Phyllanthus emblica fruit extract exerts its protective effect against cigarette-smoke induced oxidative damage through its potent antioxidant agents (gallo-ellagi tannoids).
ABSTRACT An organic acid, isolated and purified from the root extract of an Indian medicinal plan... more ABSTRACT An organic acid, isolated and purified from the root extract of an Indian medicinal plant sarsaparilla Hemidesmus indicus R. Br, possessed viper venom inhibitory activity. The compound (designated HI-RVIF) was isolated by solvent extraction, silica gel column chromatography and thin layer chromatography, and was homogeneous in nature. The white needle-shaped crystals were soluble in water, methanol and chloroform and had a melting point of 155-158 degrees C and lambda max 260 nm. Spectral analysis confirmed the presence of a benzene ring, methoxy group, and hydroxyl group; the mol. wt of the compound was 168. HI-RVIF significantly antagonized viper venom-induced lethal, haemorrhagic, coagulant and anticoagulant activity in experimental rodents.
Drug Delivery and Translational Research, Jan 6, 2020
The aim of this work was to develop a transdermal delivery system consisting of a glucosamine sul... more The aim of this work was to develop a transdermal delivery system consisting of a glucosamine sulfate-laden xanthan hydrogel containing a nanoemulsion-loaded diacerein. The system was intended to prevent cartilage degradation typical of osteoarthritis. The nanoemulsion, made of soybean oil as the oil phase; soybean lecithin, Tween 80, and poloxamer 407 as surfactants; and propylene glycol as cosurfactant, was formed within the hydrogel. The hydrodynamic diameter of the nanoemulsion globules was 81.95 ± 0.256 nm with 0.285 ± 0.036 of PDI value and the zeta potential value of the formulation was 39.33 ± 0.812 mV. CryoSEM and TEM studies revealed the uniform morphology of the vehicle. A rheological study exposed the nanoemulsionloaded hydrogel as a thixotropic system. Satisfactory storage stability under ICH conditions was established by the zeta potential and rheological studies. Furthermore, skin biocompatibility of the hydrogel was ascertained on the basis of skin irritation study. Additionally, the diffusion of the drugs across rat skin followed a controlled non-Fickian anomalous steady mechanism. Following in vivo administration in experimental osteoarthritis, the transdermal hydrogel showed a reduction in tumor necrosis factor-alpha, C-reactive protein, high mobility group box protein, and monocyte chemoattractant protein-1. Finally, histopathological analysis of the animals showed satisfactory chondroprotection in the in vivo study. In conclusion, the developed transdermal systems showed a potential against the progression of experimental osteoarthritis.
Advances in Experimental Medicine and Biology, 1996
India possessed long coastal areas where venomous catfish are abundant. But almost none of these ... more India possessed long coastal areas where venomous catfish are abundant. But almost none of these venomous fish species has been touched for detail investigation regarding their venoms and the active components present in the venom. Plotosus Canius is one of such major venomous catfish species that is present in the estuaries of eastern India. Sting of these fish produces intense pain, swelling and gangrene of the affected areas. On experimental animals the venom produced hypertension and respiratory failure, cardiac arrest, neuromuscular blockage and the LD50 was found to be 3.9 mg/kg (ip) in male albino mice (Auddy et al, 1993). An attempt was made to isolate and purify the lethal factor from the crude venom and its probable involvement in pathophysiology.
A mixture of several bacterial metabolites was used to study its effect on major immunocytes, in ... more A mixture of several bacterial metabolites was used to study its effect on major immunocytes, in vivo and in vitro. This mixture of bacterial metabolites increased number of macrophages and neutrophils and their phagocytic activity in experimental animals for a transient period. BSA induced antibody production was found to be higher in the drug treated group. These results indicated that the bacterial metabolites probably acted through non-specific defence mechanism against invading organisms and the chance of reinfection was reduced.
Journal of Chinese Integrative Medicine, Jul 15, 2011
To study the effect of Body Revival (BR), a compound traditional Indian herbal medicine, on human... more To study the effect of Body Revival (BR), a compound traditional Indian herbal medicine, on human platelet aggregation and isoproterenol (IS)-induced myocardial ischemia (MI) damage in male Wistar rats. BR suspension 10, 20 and 30 μg was mixed with platelet-rich plasma and incubated at 37 degrees centigrade for 30 min, respectively. Then, adenosine diphosphate (ADP, 20 mmol/L) or collagen (2 μg) was added in the mixture and the aggregation was observed against platelet-poor plasma mixed with equal volume of suspension of the same test samples. Wistar rats divided into 4 groups were used to investigate BR&amp;amp;amp;amp;amp;amp;#39;s effects on IS-induced MI. Levels of serum creatinine kinase (CK), aspartate transaminase (AST) and alanine transaminase (ALT) were estimated by standard commercial biological kits. Serum nitric oxide (NOx) was also measured. The lipid peroxides (LPO) and protein concentrations in heart tissues were measured. BR could inhibit ADP- or collagen-induced human platelet aggregation dose-dependently. Moreover, it could protect MI caused by IS in rats. BR reduced the levels of serum CK, AST, ALT and NOx dose-dependently and also lowered LPO in heart tissues in comparison with the MI control (P&amp;amp;amp;amp;amp;amp;lt;0.01). BR can inhibit human platelet aggregation and protect MI caused by IS in rats.
A mixture of several bacterial metabolites was used to study its effect on major immunocytes, in ... more A mixture of several bacterial metabolites was used to study its effect on major immunocytes, in vivo and in vitro. This mixture of bacterial metabolites increased number of macrophages and neutrophils and their phagocytic activity in experimental animals for a transient period. BSA induced antibody production was found to be higher in the drug treated group. These results indicated that the bacterial metabolites probably acted through non-specific defence mechanism against invading organisms and the chance of reinfection was reduced.
L'invention concerne une composition de boisson synergique permettant d'attenuer le stres... more L'invention concerne une composition de boisson synergique permettant d'attenuer le stress oxydatif, le stress hepatique, le stress du SNC et la gueule de bois suite a la consommation d'alcool, et de moduler les parametres immunologiques. La composition comprend un glycoside de saponine, un derive d'acide amine et un sucre ou un alcool de sucre en tant que principes actifs, des agents d'ajustement du pH et des agents aromatisants comme principes inactifs. Les proportions calculees du derive d'acide amine, du glycoside de saponine et du sucre ou de l'alcool de sucre presents dans l'alcool ont des effets synergiques, ce qui permet d'attenuer le stress oxydatif, le stress hepatique, le stress du SNC, la gueule de bois et de moduler les parametres immunologiques, et conduire au final a prevenir des lesions ou une atteinte d'organes suite a la consommation d'alcool, qui pourraient etre provisoires ou permanentes.
Cette invention concerne la toxicite reduite d'une composition de boisson alcoolisee fonction... more Cette invention concerne la toxicite reduite d'une composition de boisson alcoolisee fonctionnelle comprenant un alcool distille, de l'eau desionisee, de la 18β-glycyrrhizine ou 18α-glycyrrhizine et un alcool de sucre ou des sucres, ayant un pH dans la plage de 4,0 a 9,0. Plus particulierement, une composition de boisson alcoolisee comprenant un alcool distille, de l'eau desionisee, de la 18β-glycyrrhizine ou 18α-glycyrrhizine et un alcool de sucre/des sucres a titre d'agents hepato-protecteurs est en outre decrite. Cette invention donne une boisson alcoolisee destinee a reduire l'hepatotoxicite provoquee par sa consommation et un procede de fabrication de ladite boisson alcoolisee.
Aim: The point of the present investigation planned to assess the nutritive value, minerals conte... more Aim: The point of the present investigation planned to assess the nutritive value, minerals content, vitamin content, antinutritional properties, and toxicity studies in five wild-consumable plants, for example, Begonia hatacoa, Embelia floribunda, Artemisia vulgaris, Cardamine hirsuta, and Plantago major, devoured by the distinctive innate individuals of Meghalaya State in India as their nourishment. Materials and Methods: The proximate parameters such as protein, fat, fiber, sugar, minerals, and harmful overwhelming metals were assessed in the chose wild-eatable plants utilizing standard sustenance examination procedures. The cyanogenic glycoside, oxalates, tannins, saponins, and phytate substances were done to decide the counter wholesome properties of the considered plants. The quantitations of water-dissolvable vitamins in these plants were completed by high-performance liquid chromatography. The in vitro hemolytic measure of plant concentrates was completed on rodent erythrocytes. Appraisal of cytotoxicity of eatable plants was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide examine. The genotoxicity of the contemplated plants was tried by the single-cell gel electrophoresis comet assay. Results: The consequences of examination demonstrated that these plants are rich wellsprings of protein, sugar minerals, and vitamins, particularly the B gathering of vitamins. The substantial metals lead and chromium were distinguished in exceptionally low sum in all the consumable plants. The largest amount of phytate was found in P. major (0.38% ± 0.03%); oxalate was most astounding in E. floribunda (0.261% ± 0.06%) though tannin was most elevated in P. major (1.04% ± 0.12%). The estimations of antinutrients and overwhelming metals in all above-considered plants are underneath the harmful levels. Conclusion: These wild-eatable plants contribute gigantically to sustenance, nourishment security, well-being, and remedial advantages. The aftereffects of poisonous quality of every single eatable plant ensure the security at cell and genomic level and furthermore safe to expend.
Litsea cubeba is devoured by the ethnic individuals of Arunachal Pradesh in India as food and has... more Litsea cubeba is devoured by the ethnic individuals of Arunachal Pradesh in India as food and has been traditionally used for curing different ailments. The purpose of present study was to investigate the antioxidant activities of fruits of L. cubeba using different solvent extracts, quantification of phenolics, toxicity studies and DNA damage protective activities. The antioxidant activities of fruits using five different solvent extracts completed utilizing different in vitro examines. The quantitation of phenolic and polyphenolic compounds in the methanol extract of the fruits was carried out by HPLC. The in vitro haemolytic examination of plant concentrates were completed on rat erythrocytes. Appraisal of cytotoxicity of eatable fruits was assessed by MTT measure. The genotoxicity of the contemplated plant was tried by the single-cell gel electrophoresis comet measure. The DNA defensive impacts of the aqueous extracts of fruits on rodent lymphocyte DNA lesions were likewise assessed with the comet test. The extract obtained by methanol exhibited the highest antioxidant activity. The HPLC examination of the methanol concentrate of the plant demonstrated the occurrence of different phenolic acids and flavonoids like caffeic acid (145.96μg/100mg DE), syringic acid (125.85 μg/100mg DE), ferulic acid (155.89 μg/ 100mg DE), apigenin (28.43 μg/100mg DE), kaempferol (53.41 μg/100mg DE) etc. in various amounts. The consequences of haemolytic lethality, cytotoxicity and genotoxicity of fluid concentrates of the edible plant ensure the security at cell and genomic level. The fluid concentrate of the plant fundamentally repressed DNA harm and these information recommend that the watery concentrate of L. cubeba can forestall oxidative DNA harm to rodent lymphocytes, which is likely because of antioxidant constituents in the concentrate. These outcomes demonstrate that L. cubeba can be utilized in dietary applications with a possibility to diminish oxidative pressure.
The purpose of the present study was to evaluate the ability of a proprietary combination of glyc... more The purpose of the present study was to evaluate the ability of a proprietary combination of glycyrrhizin and D-mannitol to protect against oxidative damage to DNA associated with acute alcohol consumption by human subjects in a randomized, placebo-controlled cross-over designed study. Excessive alcohol consumption is associated with numerous diseases. Alcohol has been shown to generate reactive oxygen species that can result in DNA damage, leading to genetic and epigenetic changes. A total of 25 subjects (13 male and 12 female) were enrolled. Alcohol intake in the form of vodka (40% ethanol) was adjusted based on 1.275 g of 100% ethanol/kg body weight for men and 1.020 g/kg body weight for women, which was consumed with and without the study product. Blood samples were drawn at 2 h after alcohol consumption, lymphocytes were isolated, and were subjected to DNA comet electrophoresis on a blinded basis. Acute alcohol consumption increased lymphocyte DNA damage by approximately 8.36%....
... ANANYA BHAR, CHHOTAN KUMAR GHOSH, S BANDYOPADHYAY, SAMAR SARKAR, AMALENDU CHAKRABART, GAUTAM ... more ... ANANYA BHAR, CHHOTAN KUMAR GHOSH, S BANDYOPADHYAY, SAMAR SARKAR, AMALENDU CHAKRABART, GAUTAM DATTA, BISWAJIT AUDDY, PRATIP K DEBNATH. Full Text: PDF. Refbacks. There are currently no refbacks. Bookmark and Share.
India possessed long coastal areas where venomous catfish are abundant. But almost none of these ... more India possessed long coastal areas where venomous catfish are abundant. But almost none of these venomous fish species has been touched for detail investigation regarding their venoms and the active components present in the venom. Plotosus Canius is one of such major venomous catfish species that is present in the estuaries of eastern India. Sting of these fish produces intense pain, swelling and gangrene of the affected areas. On experimental animals the venom produced hypertension and respiratory failure, cardiac arrest, neuromuscular blockage and the LD50 was found to be 3.9 mg/kg (ip) in male albino mice (Auddy et al, 1993). An attempt was made to isolate and purify the lethal factor from the crude venom and its probable involvement in pathophysiology.
The venom of the common Indian catfish P. canius Hamilton (locally called 'Kanmagur') was examine... more The venom of the common Indian catfish P. canius Hamilton (locally called 'Kanmagur') was examined for its pharmacodynamic activity. The LD50 of the venom in mice was found to be 3.9 mg/kg (ip). At lower doses, the venom produced a positive inotropic effect on toad and rabbit hearts, while at higher doses it produced cardiac arrest. In the isolated guinea pig auricle, the venom increased the rate and amplitude of contraction. The venom increased rat blood pressure--an action antagonised by alpha-adrenergic blocker (phenoxybenzamine). It reduced the rate and amplitude of rat and guinea pig respiration leading to respiratory arrest and death. The venom did not alter the cutaneous capillary permeability of guinea pig but produced vasoconstrictor effect on rat hindquarter perfusion. It induced contractions in several smooth muscle preparations viz., ileum and colon of guinea pig, fundus, uterus and ileum of rat. On isolated guinea pig ileum, the venom produced contraction which was not antagonised by atropine and mepyramine, but was partially antagonised by methysergide associated with a residual contraction which was abolished by SC 19220, a prostaglandin receptor blocker. The venom produced irreversible blockade of electrically induced twitch response on isolated rat phrenic nerve diaphragm and chick biventer cervicis preparation. Haemolysis was not produced by the venom on mice, guinea pig and human RBC (washed).
Aim: To study wound healing activity of the human placental extract (HPE) in rats. Methods: Full ... more Aim: To study wound healing activity of the human placental extract (HPE) in rats. Methods: Full thickness wounds were inflicted on depilated dorsum of Charles foster rats with 8 mm Acu-punch biopsy. The HPE was applied both at topical and im routes (2.5 mL/kg). Effects were compared on the basis of physical criteria, biochemical criteria, and histopathological study with respect to untreated control, vehicle control (1.5 % benzyl alcohol), and framycetin topical treated groups. Results: Significant lowering of wound size (P<0.05), wound index (P<0.05), and number of days required for complete healing (P<0.01); significant gain in tensile strength (P<0.01); appreciable increase of tissue DNA, total protein, and collagenesis were observed in HPE treated group. Conclusion: Human placental extract systematically helps collagenesis leading to potent healing of wounds.
Cigarette smoke contains oxidants which generate long lasting free radicals that cause oxidative ... more Cigarette smoke contains oxidants which generate long lasting free radicals that cause oxidative stress in active and passive smokers leading to pathological conditions. Antioxidant nutrients are reported to prevent the oxidative damage induced by smoking. In the present study, we have evaluated the antioxidant role of Phyllanthus emblica (PE) fruit extract on cigarette smoke-induced oxidative stress in rats. Adult male albino rats were exposed to cigarette smoke and administered daily with PE fruit extract (Gr.A) or vitamin supplements (Gr.D). Another group of animals were exposed to only cigarette smoke (Gr.B) and control animals were subjected to sham session (Gr.C). Group A, B and D animals were kept in a smoke chamber for 30 min, twice a day for 4 weeks. Hemoglobin (Hb) % and malondialdehyde (MDA) content of RBC, lung and liver homogenate; superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and glutathione-S-transferases (GST) of lung and liver homogenate of different treatment groups were estimated. Administration of PE fruit extract showed increase in the Hb level (+ 23.4 %) of Gr.A animals with concomitant decrease in MDA levels of RBC, lung and liver homogenate (48-54%, p<0.001) when compared to those of group B animals. The status of improvement of SOD, CAT, GSH and GST of lung and liver homogenate in PE fruit extract-administered group (Gr.A) ranged from 30-80% over the only smoke exposed group (Gr.B). PE fruit extract administered group showed better antioxidant profiles than that of the multivitamin supplemented group. The results of the present study suggest that Phyllanthus emblica fruit extract exerts its protective effect against cigarette-smoke induced oxidative damage through its potent antioxidant agents (gallo-ellagi tannoids).
ABSTRACT An organic acid, isolated and purified from the root extract of an Indian medicinal plan... more ABSTRACT An organic acid, isolated and purified from the root extract of an Indian medicinal plant sarsaparilla Hemidesmus indicus R. Br, possessed viper venom inhibitory activity. The compound (designated HI-RVIF) was isolated by solvent extraction, silica gel column chromatography and thin layer chromatography, and was homogeneous in nature. The white needle-shaped crystals were soluble in water, methanol and chloroform and had a melting point of 155-158 degrees C and lambda max 260 nm. Spectral analysis confirmed the presence of a benzene ring, methoxy group, and hydroxyl group; the mol. wt of the compound was 168. HI-RVIF significantly antagonized viper venom-induced lethal, haemorrhagic, coagulant and anticoagulant activity in experimental rodents.
Drug Delivery and Translational Research, Jan 6, 2020
The aim of this work was to develop a transdermal delivery system consisting of a glucosamine sul... more The aim of this work was to develop a transdermal delivery system consisting of a glucosamine sulfate-laden xanthan hydrogel containing a nanoemulsion-loaded diacerein. The system was intended to prevent cartilage degradation typical of osteoarthritis. The nanoemulsion, made of soybean oil as the oil phase; soybean lecithin, Tween 80, and poloxamer 407 as surfactants; and propylene glycol as cosurfactant, was formed within the hydrogel. The hydrodynamic diameter of the nanoemulsion globules was 81.95 ± 0.256 nm with 0.285 ± 0.036 of PDI value and the zeta potential value of the formulation was 39.33 ± 0.812 mV. CryoSEM and TEM studies revealed the uniform morphology of the vehicle. A rheological study exposed the nanoemulsionloaded hydrogel as a thixotropic system. Satisfactory storage stability under ICH conditions was established by the zeta potential and rheological studies. Furthermore, skin biocompatibility of the hydrogel was ascertained on the basis of skin irritation study. Additionally, the diffusion of the drugs across rat skin followed a controlled non-Fickian anomalous steady mechanism. Following in vivo administration in experimental osteoarthritis, the transdermal hydrogel showed a reduction in tumor necrosis factor-alpha, C-reactive protein, high mobility group box protein, and monocyte chemoattractant protein-1. Finally, histopathological analysis of the animals showed satisfactory chondroprotection in the in vivo study. In conclusion, the developed transdermal systems showed a potential against the progression of experimental osteoarthritis.
Advances in Experimental Medicine and Biology, 1996
India possessed long coastal areas where venomous catfish are abundant. But almost none of these ... more India possessed long coastal areas where venomous catfish are abundant. But almost none of these venomous fish species has been touched for detail investigation regarding their venoms and the active components present in the venom. Plotosus Canius is one of such major venomous catfish species that is present in the estuaries of eastern India. Sting of these fish produces intense pain, swelling and gangrene of the affected areas. On experimental animals the venom produced hypertension and respiratory failure, cardiac arrest, neuromuscular blockage and the LD50 was found to be 3.9 mg/kg (ip) in male albino mice (Auddy et al, 1993). An attempt was made to isolate and purify the lethal factor from the crude venom and its probable involvement in pathophysiology.
A mixture of several bacterial metabolites was used to study its effect on major immunocytes, in ... more A mixture of several bacterial metabolites was used to study its effect on major immunocytes, in vivo and in vitro. This mixture of bacterial metabolites increased number of macrophages and neutrophils and their phagocytic activity in experimental animals for a transient period. BSA induced antibody production was found to be higher in the drug treated group. These results indicated that the bacterial metabolites probably acted through non-specific defence mechanism against invading organisms and the chance of reinfection was reduced.
Journal of Chinese Integrative Medicine, Jul 15, 2011
To study the effect of Body Revival (BR), a compound traditional Indian herbal medicine, on human... more To study the effect of Body Revival (BR), a compound traditional Indian herbal medicine, on human platelet aggregation and isoproterenol (IS)-induced myocardial ischemia (MI) damage in male Wistar rats. BR suspension 10, 20 and 30 μg was mixed with platelet-rich plasma and incubated at 37 degrees centigrade for 30 min, respectively. Then, adenosine diphosphate (ADP, 20 mmol/L) or collagen (2 μg) was added in the mixture and the aggregation was observed against platelet-poor plasma mixed with equal volume of suspension of the same test samples. Wistar rats divided into 4 groups were used to investigate BR&amp;amp;amp;amp;amp;amp;#39;s effects on IS-induced MI. Levels of serum creatinine kinase (CK), aspartate transaminase (AST) and alanine transaminase (ALT) were estimated by standard commercial biological kits. Serum nitric oxide (NOx) was also measured. The lipid peroxides (LPO) and protein concentrations in heart tissues were measured. BR could inhibit ADP- or collagen-induced human platelet aggregation dose-dependently. Moreover, it could protect MI caused by IS in rats. BR reduced the levels of serum CK, AST, ALT and NOx dose-dependently and also lowered LPO in heart tissues in comparison with the MI control (P&amp;amp;amp;amp;amp;amp;lt;0.01). BR can inhibit human platelet aggregation and protect MI caused by IS in rats.
A mixture of several bacterial metabolites was used to study its effect on major immunocytes, in ... more A mixture of several bacterial metabolites was used to study its effect on major immunocytes, in vivo and in vitro. This mixture of bacterial metabolites increased number of macrophages and neutrophils and their phagocytic activity in experimental animals for a transient period. BSA induced antibody production was found to be higher in the drug treated group. These results indicated that the bacterial metabolites probably acted through non-specific defence mechanism against invading organisms and the chance of reinfection was reduced.
L'invention concerne une composition de boisson synergique permettant d'attenuer le stres... more L'invention concerne une composition de boisson synergique permettant d'attenuer le stress oxydatif, le stress hepatique, le stress du SNC et la gueule de bois suite a la consommation d'alcool, et de moduler les parametres immunologiques. La composition comprend un glycoside de saponine, un derive d'acide amine et un sucre ou un alcool de sucre en tant que principes actifs, des agents d'ajustement du pH et des agents aromatisants comme principes inactifs. Les proportions calculees du derive d'acide amine, du glycoside de saponine et du sucre ou de l'alcool de sucre presents dans l'alcool ont des effets synergiques, ce qui permet d'attenuer le stress oxydatif, le stress hepatique, le stress du SNC, la gueule de bois et de moduler les parametres immunologiques, et conduire au final a prevenir des lesions ou une atteinte d'organes suite a la consommation d'alcool, qui pourraient etre provisoires ou permanentes.
Cette invention concerne la toxicite reduite d'une composition de boisson alcoolisee fonction... more Cette invention concerne la toxicite reduite d'une composition de boisson alcoolisee fonctionnelle comprenant un alcool distille, de l'eau desionisee, de la 18β-glycyrrhizine ou 18α-glycyrrhizine et un alcool de sucre ou des sucres, ayant un pH dans la plage de 4,0 a 9,0. Plus particulierement, une composition de boisson alcoolisee comprenant un alcool distille, de l'eau desionisee, de la 18β-glycyrrhizine ou 18α-glycyrrhizine et un alcool de sucre/des sucres a titre d'agents hepato-protecteurs est en outre decrite. Cette invention donne une boisson alcoolisee destinee a reduire l'hepatotoxicite provoquee par sa consommation et un procede de fabrication de ladite boisson alcoolisee.
Aim: The point of the present investigation planned to assess the nutritive value, minerals conte... more Aim: The point of the present investigation planned to assess the nutritive value, minerals content, vitamin content, antinutritional properties, and toxicity studies in five wild-consumable plants, for example, Begonia hatacoa, Embelia floribunda, Artemisia vulgaris, Cardamine hirsuta, and Plantago major, devoured by the distinctive innate individuals of Meghalaya State in India as their nourishment. Materials and Methods: The proximate parameters such as protein, fat, fiber, sugar, minerals, and harmful overwhelming metals were assessed in the chose wild-eatable plants utilizing standard sustenance examination procedures. The cyanogenic glycoside, oxalates, tannins, saponins, and phytate substances were done to decide the counter wholesome properties of the considered plants. The quantitations of water-dissolvable vitamins in these plants were completed by high-performance liquid chromatography. The in vitro hemolytic measure of plant concentrates was completed on rodent erythrocytes. Appraisal of cytotoxicity of eatable plants was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide examine. The genotoxicity of the contemplated plants was tried by the single-cell gel electrophoresis comet assay. Results: The consequences of examination demonstrated that these plants are rich wellsprings of protein, sugar minerals, and vitamins, particularly the B gathering of vitamins. The substantial metals lead and chromium were distinguished in exceptionally low sum in all the consumable plants. The largest amount of phytate was found in P. major (0.38% ± 0.03%); oxalate was most astounding in E. floribunda (0.261% ± 0.06%) though tannin was most elevated in P. major (1.04% ± 0.12%). The estimations of antinutrients and overwhelming metals in all above-considered plants are underneath the harmful levels. Conclusion: These wild-eatable plants contribute gigantically to sustenance, nourishment security, well-being, and remedial advantages. The aftereffects of poisonous quality of every single eatable plant ensure the security at cell and genomic level and furthermore safe to expend.
Litsea cubeba is devoured by the ethnic individuals of Arunachal Pradesh in India as food and has... more Litsea cubeba is devoured by the ethnic individuals of Arunachal Pradesh in India as food and has been traditionally used for curing different ailments. The purpose of present study was to investigate the antioxidant activities of fruits of L. cubeba using different solvent extracts, quantification of phenolics, toxicity studies and DNA damage protective activities. The antioxidant activities of fruits using five different solvent extracts completed utilizing different in vitro examines. The quantitation of phenolic and polyphenolic compounds in the methanol extract of the fruits was carried out by HPLC. The in vitro haemolytic examination of plant concentrates were completed on rat erythrocytes. Appraisal of cytotoxicity of eatable fruits was assessed by MTT measure. The genotoxicity of the contemplated plant was tried by the single-cell gel electrophoresis comet measure. The DNA defensive impacts of the aqueous extracts of fruits on rodent lymphocyte DNA lesions were likewise assessed with the comet test. The extract obtained by methanol exhibited the highest antioxidant activity. The HPLC examination of the methanol concentrate of the plant demonstrated the occurrence of different phenolic acids and flavonoids like caffeic acid (145.96μg/100mg DE), syringic acid (125.85 μg/100mg DE), ferulic acid (155.89 μg/ 100mg DE), apigenin (28.43 μg/100mg DE), kaempferol (53.41 μg/100mg DE) etc. in various amounts. The consequences of haemolytic lethality, cytotoxicity and genotoxicity of fluid concentrates of the edible plant ensure the security at cell and genomic level. The fluid concentrate of the plant fundamentally repressed DNA harm and these information recommend that the watery concentrate of L. cubeba can forestall oxidative DNA harm to rodent lymphocytes, which is likely because of antioxidant constituents in the concentrate. These outcomes demonstrate that L. cubeba can be utilized in dietary applications with a possibility to diminish oxidative pressure.
The purpose of the present study was to evaluate the ability of a proprietary combination of glyc... more The purpose of the present study was to evaluate the ability of a proprietary combination of glycyrrhizin and D-mannitol to protect against oxidative damage to DNA associated with acute alcohol consumption by human subjects in a randomized, placebo-controlled cross-over designed study. Excessive alcohol consumption is associated with numerous diseases. Alcohol has been shown to generate reactive oxygen species that can result in DNA damage, leading to genetic and epigenetic changes. A total of 25 subjects (13 male and 12 female) were enrolled. Alcohol intake in the form of vodka (40% ethanol) was adjusted based on 1.275 g of 100% ethanol/kg body weight for men and 1.020 g/kg body weight for women, which was consumed with and without the study product. Blood samples were drawn at 2 h after alcohol consumption, lymphocytes were isolated, and were subjected to DNA comet electrophoresis on a blinded basis. Acute alcohol consumption increased lymphocyte DNA damage by approximately 8.36%....
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