Background: Maternal stress before, during and after pregnancy has profound effects on the develo... more Background: Maternal stress before, during and after pregnancy has profound effects on the development and lifelong function of the infant's neurocognitive development. We hypothesized that the programming of the central nervous system (CNS), hypothalamic-pituitary-adrenal (HPA) axis and autonomic nervous system (ANS) induced by prenatal stress (PS) is reflected in electrophysiological and epigenetic biomarkers. In this study, we aimed to find noninvasive epigenetic biomarkers of PS in the newborn salivary DNA. Results: A total of 728 pregnant women were screened for stress exposure using Cohen Perceived Stress Scale (PSS), 164 women were enrolled, and 114 dyads were analyzed. Prenatal Distress Questionnaire (PDQ) was also administered to assess specific pregnancy worries. Transabdominal fetal electrocardiograms (taECG) were recorded to derive coupling between maternal and fetal heart rates resulting in a 'Fetal Stress Index' (FSI). Upon delivery, we collected maternal hair strands for cortisol measurements and newborn's saliva for epigenetic analyses. DNA was extracted from saliva samples, and DNA methylation was measured using EPIC BeadChip array (850 k CpG sites). Linear regression was used to identify associations between PSS/PDQ/FSI/Cortisol and DNA methylation. We found epigenome-wide significant associations for 5 CpG with PDQ and cortisol at FDR < 5%. Three CpGs were annotated to genes (Illumina Gene annotation file): YAP1, TOMM20 and CSMD1, and two CpGs were located approximately lay at 50 kb from SSBP4 and SCAMP1. In addition, two differentiated methylation regions (DMR) related to maternal stress measures PDQ and cortisol were found: DAXX and ARL4D. Conclusions: Genes annotated to these CpGs were found to be involved in secretion and transportation, nuclear signaling, Hippo signaling pathways, apoptosis, intracellular trafficking and neuronal signaling. Moreover, some CpGs are annotated to genes related to autism, post-traumatic stress disorder (PTSD) and schizophrenia. However, our results should be viewed as hypothesis generating until replicated in a larger sample. Early assessment of such noninvasive PS biomarkers will allow timelier detection of babies at risk and a more effective allocation of resources for early
The aim of the study was to evaluate adrenal axis hyperactivation measuring hair cortisol levels,... more The aim of the study was to evaluate adrenal axis hyperactivation measuring hair cortisol levels, and its influence on the relationship among metabolic parameters, inflammation markers and androgens in adult women with PCOS. 44 women (18–34 years) with PCOS diagnosis and a control group of 49 healthy women (19–35 years) were included. In both gropus body mass index (BMI) was calculated and waist circumference (WC) was measured. Hair cortisol, total serum testosterone (TT), serum cortisol, 25 OH vitamin D (25OHD), insulin, high sensitivity C-reactive protein (hsCRP), triglycerides (TG), HDL cholesterol (HDL), glucose and leptin were measured. Bioavailable testosterone (bioT) was calculated. Hair cortisol concentration was higher and significantly different in PCOS patients compared to the control group (130 vs 63 pg/mg of hair, p < 0.001). Subsequently, patients with PCOS were divided into two groups according to hair cortisol levels: group 1 with normal hair cortisol concentratio...
Cortisol is considered one of the most useful parameters to evaluate alterations of the hypothala... more Cortisol is considered one of the most useful parameters to evaluate alterations of the hypothalamic-pituitary-adrenal (HHA) axis. Measurement of serum, salivary and urinary cortisol only provides information of short periods of time, therefore alternative matrices such as hair are proposed as it would reflect cortisol levels of the last 3 months. The aim of this study was to evaluate the association of chronic stress with different pathologies that involve a deregulation of HHA axis through the measurement of hair cortisol by an automated method. The study population consisted of 286 individuals, 232 healthy individuals without medication or adrenal pathology, of whom 213 individuals were healthy and stress-free according to Holmes-Rahe life events scale. In healthy stress-free individuals, median hair cortisol was 55 pg/mg of hair (40-173), whereas in stressed individuals it was 250 pg/mg of hair (182-520). The reference interval was 40-128 pg/mg of hair. In order to assess the cl...
HIV infection is a major risk factor predisposing for Mycobacterium tuberculosis infection and pr... more HIV infection is a major risk factor predisposing for Mycobacterium tuberculosis infection and progression to active tuberculosis (TB). As host immune response defines the course of infection, we aimed to identify immuno-endocrine changes over six-months of anti-TB chemotherapy in HIV+ people. Plasma levels of cortisol, DHEA and DHEA-S, percentages of CD4+ regulatory T cell subsets and number of IFN-γ-secreting cells were determined. Several cytokines, chemokines and C-reactive protein levels were measured. Results were correlated with clinical parameters as predictors of infection resolution and compared to similar data from HIV+ individuals, HIV-infected persons with latent TB infection and healthy donors. Throughout the course of anti-TB/HIV treatment, DHEA and DHEA-S plasma levels raised while cortisol diminished, which correlated to predictive factors of infection resolution. Furthermore, the balance between cortisol and DHEA, together with clinical assessment, may be considere...
In the critical context of COVID-19 pandemic, healthcare workers are on the front line, participa... more In the critical context of COVID-19 pandemic, healthcare workers are on the front line, participating directly in the care, diagnosis, and treatment of patients with COVID-19. This exposes them to a higher risk of developing chronic stress, psychological distress, and any other mental health symptoms. Objective: to evaluate stress and burnout in a health workers population and, in addition, to measure hair cortisol concentration as a current biomarker of stress. Materials and methods: 234 health workers from Hospital de Clínicas "José de San Martín", Buenos Aires University, were included in this study. In this population hair samples were obtained from the posterior vertex as close to the scalp as possible and the individuals completed the following surveys: perceived stress, social support, burnout scale, life event scale, and sociodemographic data. Hair cortisol was measured by an automated chemiluminescent method. The studied population was divided into three groups considering those individuals below the healthy reference sample range (< 40 pg/mg hair), within the healthy reference range (40-128 pg/mg hair) and above the reference range (> 128 pg/mg hair). This study used a transversal and observational design. Results: Our results show that 40% of the studied population presented hair cortisol values outside of the healthy reference range. In the whole studied population, a direct correlation was found between hair cortisol concentration and perceived stress as well as between hair cortisol concentration and the emotional exhaustion component of burnout (r = 0.142, p = 0.030; r = 0.143, p = 0.029, respectively). 12% of the studied population showed Burnout (52% doctors and residents, 19% nurses, 19% administrative personnel). Higher values in hair cortisol levels were found in the group with burnout versus individuals without burnout (p = 0.034). Finally, a mediation analysis was performed, finding that depersonalization is a mediating variable in the relationship between self-perceived stress and hair cortisol level (F = 4.86, p = 0.0086; indirect effect IC: 0.0987-1.8840). Conclusion: This is the first study in which a stress biomarker such as hair cortisol is evaluated in this population and in this context. Healthcare workers are subjected to increased levels of stress and burnout. High depersonalization, emotional exhaustion, and decreased personal sense of accomplishment characterize this population. It is the responsibility of the health authorities to implement strategies to manage this psychological emergency.
American Journal of Clinical and Experimental Medicine, 2019
The diagnostic procedure for the evaluation of Cushing's syndrome is performed by any of the foll... more The diagnostic procedure for the evaluation of Cushing's syndrome is performed by any of the following biochemical tests: urine free cortisol, salivary cortisol at 11 pm and serum cortisol post 1 mg of dexamethasone. Collection of saliva samples is simple and noninvasive, thus being a method of choice for the evaluation of risk populations. The aim of this work is to analyze the performance of an automated chemiluminescent method for measurement of salivary cortisol at 11 pm according to the new quality guidelines and assess its clinical utility. Cortisol levels were measured in samples obtained by passive drooling from 32 healthy subjects and 9 patients with Cushing's syndrome. Matrix effect, linearity, limit of blank, limit of quantitation, recovery and diagnostic performance were assessed. The Unicel 600 DXI Access Beckman Coulter chemiluminescent automated analyzer was used. The standard curve provided by the manufacturer was adapted to measure cortisol concentrations in saliva. Matrix effect: equation of the curve using salivary matrix: y=-1.824x+3.491 (95% CI=-2.068 to-1.582) vs. Equation of the curve using diluent matrix: y=-1.833x+3.394 (95% CI=-1.961 to-1.704). There is overlapping of both curves.
Insulin-like growth factor I (IGF-I) represents an essential tool in the diagnostic work-up and i... more Insulin-like growth factor I (IGF-I) represents an essential tool in the diagnostic work-up and in the monitoring of treatment efficacy for somatotrophic axis disorders both in children and adults. A large number of factors including, but not limited to, age, sex and weight as well as analytical variables influence IGF-I serum levels; therefore, reliable normative data are essential for a correct interpretation of results. The aim of the present study was to establish reference range values for serum IGF-I, in a large population of healthy adults from Buenos Aires city. The study included serum samples from 1044 healthy subjects aged 21-87 years (423 females and 621 males) divided into groups by sex at 5-year intervals from 21 to &gt;75 years. Serum IGF-I concentrations were determined by a fully automated two-site, solid-phase, enzyme-labeled chemiluminescent immunometric assay (Immulite 2000, Siemens Healthcare Diagnostics). IGF-BP interferences are circumvented by blocking IGF-BP binding sites with excess IGF-II in the on-board predilution step. Results show the age dependence of circulating IGF-I levels, with a smooth and steady decrease in levels with age. No sex differences were found in subjects &gt;26 years; however, in the group aged 21-25 years, IGF-I levels were significantly higher in females. In conclusion, this study provides age- and gender-adjusted normal reference ranges for IGF-I levels obtained with an automated immunometric chemiluminescent assay Immulite 2000 in healthy adult subjects.
To evaluate the relationship between testosterone levels and the metabolic syndrome (MS) in men o... more To evaluate the relationship between testosterone levels and the metabolic syndrome (MS) in men older than 45 years. Six hundred and sixty men (45-70 years) selected from 2906 participants of a population screening for prostate cancer were included in this study. Testosterone and the components of MS were assessed in all men. MS was diagnosed according to NCEP-ATP III criteria. Triglycerides (TG)/HDL-cholesterol (chol) index was calculated. The presence of MS was inversely associated with testosterone (χ2, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001), independently of age (OR 0.802, CI 95%: 0.724-0.887, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001). Hypertension was the most frequent abnormality observed followed by elevated TG and waist circumference (WC). Testosterone correlated positively with HDL-chol (r: 0.14, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001) and negatively with body mass index (BMI)(r: -0.29, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), WC (r: -0.26, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), TG (r: -0.20, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), TG/HDL-chol (r: -0.20, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), glucose (r: -0.11, p = 0.005) and MS score (r: -0.23, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001). Our results show that in men older than 45 years, as long as testosterone levels decline, the prevalence of MS increases, independently of age. The correlations found between testosterone and four of the five components of MS, as well as with BMI and TG/HDL-chol ratio, a surrogate marker of insulin resistance, suggest considering male hypogonadism as a determinant of developmental abnormalities typical of MS.
Introducción: El cáncer de próstata (CaP) es la tercera causa de muerte oncológica en el varón. E... more Introducción: El cáncer de próstata (CaP) es la tercera causa de muerte oncológica en el varón. En el paciente obeso hay múltiples alteraciones hormonales que se podrían relacionar con el aumento de la incidencia del CaP. Objetivo: Estudiar la relación entre el perfil lipoproteico y la obesidad y su relación como factores asociados con el CaP. Materiales y Métodos: Se seleccionaron 150 pacientes de entre 50 y 65 años: 50 con CaP, 50 con Hiperplasia Prostática Benigna (HPB) y 50 sin patología prostática. Se les realizó un dosaje de Antígeno Prostático Específico total (PSAt) y Examen Digital Rectal (EDR). Resultados: Los pacientes con CaP presentaron valores de PSAt mayores que las otras dos poblaciones. No hubo diferencias en el Índice de Masa Corporal (IMC) entre los tres grupos. Los pacientes con CaP presentaron valores disminuidos de Colesterol-HDL y una relación Triglicéridos/Colesterol-HDL más alta. Los pacientes con HPB presentaron menores valores de Colesterol-total con respecto a los controles, sin diferencias con respecto a los pacientes con CaP. Se encontraron correlaciones inversas significativas entre el PSAt y el Colesterol-total y el Colesterol-HDL. Conclusiones: El descenso de colesterol-total en los pacientes con CaP se atribuiría al descenso de colesterol-HDL, con el consiguiente aumento de la relación triglicéridos/col-HDL, la cual sería un marcador de insulino-resistencia.
To evaluate lipoprotein profile and sex hormones in patients with prostate cancer (PCa) and benig... more To evaluate lipoprotein profile and sex hormones in patients with prostate cancer (PCa) and benign prostatic hyperplasia (BPH) and their possible associations with some inflammatory markers linked to PCa. A total of 150 men (50-65 years), matched by age and body mass index (BMI), included in this study and divided into three groups according to total prostate specific antigen (PSA), digital rectal examination and prostate biopsy: 50 PCa, 50 BPH and 50 controls. Total cholesterol (Chol), HDL-chol, LDL-chol, triglycerides (TG), total testosterone (T), free T (FT), bioavailable T (BioT), estradiol and SHBG were measured. The free androgen index (FAI) and TG/HDL-chol were calculated. In 25 PCa and 25 controls, C-reactive protein (hs-CRP), adiponectin and insulin were determined. Patients with PCa showed higher TG/HDL-chol and diminished HDL-chol than Controls and BPH. PSA correlated inversely with HDL-chol and directly with TG/HDL-chol. FAI, FT, BioT and estradiol levels were higher, and SHBG and adiponectin were lower in PCa than in Controls. No differences were found in androgens between BPH and PCa. Our most novel findings are that the patients with PCa presented lower total Chol and HDL-chol and higher TG/HDL-chol than BPH and Controls. Patients with PCa showed higher androgens and lower adiponectin than Controls.
Objectives — The objective of our study was to assess humoral response in a population of health ... more Objectives — The objective of our study was to assess humoral response in a population of health workers after vaccination with the first and second doses of Sputnik V. Methods — SARS-CoV-2 total antibodies (IgG and IgM) were measured, using the Centaur XPT autoanalyzer, Siemens®, in 530 serum samples taken from health workers in Buenos Aires vaccinated with Sputnik V. Results — After 21 days of the first dose application, 10 individuals (1.9%) presented antibody levels <1.0 (non-reactive), while 520 subjects (98.1%) responded with antibody values >1.0 (reactive). The results, obtained 21 days after the second dose, show that only 2 individuals (0.38%) had antibody levels <1.0 (non-reactive) and 528 (99.6%) responded with antibody values >1.0 (reactive). Conclusion — This study results implied that two doses of Sputnik V vaccine generated a proper antibody response in virtually the entire studied population.
The adverse effects of maternal prenatal stress (PS) on child’s neurodevelopment warrant the esta... more The adverse effects of maternal prenatal stress (PS) on child’s neurodevelopment warrant the establishment of biomarkers that enable early interventional therapeutic strategies. We performed a prospective matched double cohort study screening 2000 pregnant women in third trimester with Cohen Perceived Stress Scale-10 (PSS-10) questionnaire; 164 participants were recruited and classified as stressed and control group (SG, CG). Fetal cord blood iron parameters were measured at birth. Transabdominal electrocardiograms-based Fetal Stress Index (FSI) was derived. We investigated sex contribution to group differences and conducted causal inference analyses to assess the total effect of PS exposure on iron homeostasis using a directed acyclic graph (DAG) approach. Differences are reported for p<0.05 unless noted otherwise. Transferrin saturation was lower in male stressed neonates. The minimum adjustment set of the DAG to estimate the total effect of PS exposure on fetal ferritin iron b...
In the pregnant mother and her fetus, chronic prenatal stress results in entrainment of the fetal... more In the pregnant mother and her fetus, chronic prenatal stress results in entrainment of the fetal heartbeat by the maternal heartbeat, quantified by the fetal stress index (FSI). Deep learning (DL) is capable of pattern detection in complex medical data with high accuracy in noisy real-life environments, but little is known about DL’s utility in non-invasive biometric monitoring during pregnancy. A recently established self-supervised learning (SSL) approach to DL provides emotional recognition from electrocardiogram (ECG). We hypothesized that SSL will identify chronically stressed mother-fetus dyads from the raw maternal abdominal electrocardiograms (aECG), containing fetal and maternal ECG. Chronically stressed mothers and controls matched at enrolment at 32 weeks of gestation were studied. We validated the chronic stress exposure by psychological inventory, maternal hair cortisol and FSI. We tested two variants of SSL architecture, one trained on the generic ECG features for emo...
Revista de la Sociedad Argentina de Diabetes, 2020
Introducción: la vía inflamatoria JNK (c-Jun N-terminal Kinase) desempeña un rol central en el de... more Introducción: la vía inflamatoria JNK (c-Jun N-terminal Kinase) desempeña un rol central en el desarrollo de neuroinflamación y diabetes mellitus tipo 2 (DM2). La inflamación subclínica es una característica emblemática de DM2 y entidades asociadas. Se acompaña de activación de macrófagos que, de manera ubicua, infiltran y dañan una miríada de órganos, incluido SNC y páncreas. Dichos macrófagos descienden de monocitos inflamatorios que sobreexpresan, precozmente, la vía JNK, incluso en personas no diabéticas, con riesgo de DM2. Aunque se conoce que obesidad y DM2 afectan el SNC, dicha asociación es compleja. Estudios longitudinales demostraron que un elevado coeficiente intelectual, en niños, se asocia a menor obesidad en la adultez a partir de un estilo de vida saludable, sugiriendo que el eje diabesidad-SNC sería bidireccional.Objetivos: explorar interrelaciones entre la expresión monocitaria de JNK, la velocidad de reacción psicomotora (medida correlacionada con la inteligencia) ...
In a pregnant mother and her fetus, chronic prenatal stress results in entrainment of the fetal h... more In a pregnant mother and her fetus, chronic prenatal stress results in entrainment of the fetal heartbeat by the maternal heartbeat, quantified by the fetal stress index (FSI). Deep learning (DL) is capable of pattern detection in complex medical data with high accuracy in noisy real-life environments, but little is known about the utility of DL in non-invasive biometrics during pregnancy. We hypothesized that a recently established self-supervised learning (SSL) approach that provides emotional recognition from ECG will identify chronically stressed mother-fetus dyads from the raw maternal abdominal electrocardiograms (aECG), containing fetal and maternal ECG. Chronically stressed mothers and controls matched on enrolment at 32 weeks of gestation were studied. We validated the chronic stress exposure by psychological inventory, maternal hair cortisol, and FSI. We tested two variants of SSL architecture, one trained on the generic ECG features for emotional recognition obtained from...
Asian Pacific journal of cancer prevention : APJCP, 2016
Prostate cancer (PCa) is one of the most common diseases in men. It is important to assess progno... more Prostate cancer (PCa) is one of the most common diseases in men. It is important to assess prognostic factors and whether high cortisol levels and complex hormonal interactions could be responsible for PCa development. We evaluated the relationship between cortisol, leptin and estrogens in 141 men, 71 with PCa and the remaining 70 constituting a low risk group (LRG). They were recruited for this study from a total of 2906 middleaged men (ages 4570 years) who completed an evaluation for prostatic diseases at the Urology Division, Hospital de Clinicas "Jose de San Martin", University of Buenos Aires, in May 2009. In this cross sectional study, cortisol, PSA, totaltestosterone, freetestosterone, bioavailable testosterone, LH and estradiol were measured in serum. We observed increased cortisol levels in PCa patients as compared to LRG cases (p=0.004,). Leptin and estradiol levels were also higher in PCa patients (p=0.048; p<0.0001, respectively). Logistic regression analysi...
Revista de la Sociedad Argentina de Diabetes, 2020
Introducción: la depresión (DP) tiene una alta prevalencia en pacientes con diabetes mellitus tip... more Introducción: la depresión (DP) tiene una alta prevalencia en pacientes con diabetes mellitus tipo 1 (DM1) y se asocia a repercusiones clínicas negativas como mayor morbimortalidad cardiovascular y complicaciones crónicas. Existen pocos estudios publicados sobre la funcionalidad del eje hipotálamo-hipófiso-adrenal (H-H-A) en DM1 con DP, y la relación entre la DP y el test de respuesta del cortisol al despertar (RCD) con el control glucémico (CG).Objetivos: analizar la funcionalidad del eje H-H-A a través de la evaluación del RCD en pacientes con DM1 (PD1) con y sin DP. Como objetivos secundarios, conocer la prevalencia de DP en PD1 y ver si existe relación entre el RCD y CG y entre DP y CG.Materiales y métodos: estudio observacional, prospectivo, de corte transversal, multicéntrico, nacional. Se incluyeron PD1 mayores de 18 años; se utilizó cuestionario Patient Health Questionnaire-9 (PHQ-9) para diagnóstico de DP. Se tomaron muestras de cortisol salival al despertar y a los 30 minu...
Introduccion: La hiperplasia prostatica benigna (HPB) constituye una patologia con alta prevalenc... more Introduccion: La hiperplasia prostatica benigna (HPB) constituye una patologia con alta prevalencia en hombres mayores, y en su patogenia es posible la influencia de la inflamacion prostatica. La resistencia a la insulina, que se presenta con hiperinsulinemia (que forma parte del sindrome metabolico [SM]) y el factor de crecimiento simil insulina tipo 1 (insulin-like growth factor-1, IGF-1) (relacionado con la obesidad) se asocian al aumento de sintomas urinarios por inflamacion prostatica y crecimiento prostatico debido a que influyen sobre vias de senalizacion que generan proliferacion celular. A su vez, la leptina (tambien elevada en la obesidad) poseeria efectos sobre el crecimiento celular. Objetivo: Evaluar la relacion entre estas tres moleculas (resistencia a la insulina, IGF-1 y leptina) con la hiperplasia prostatica benigna. Material y metodo: Poblacion seleccionada de una convocatoria realizada bajo la denominacion “Campana de deteccion temprana del cancer de prostata”. Se...
Background: Maternal stress before, during and after pregnancy has profound effects on the develo... more Background: Maternal stress before, during and after pregnancy has profound effects on the development and lifelong function of the infant's neurocognitive development. We hypothesized that the programming of the central nervous system (CNS), hypothalamic-pituitary-adrenal (HPA) axis and autonomic nervous system (ANS) induced by prenatal stress (PS) is reflected in electrophysiological and epigenetic biomarkers. In this study, we aimed to find noninvasive epigenetic biomarkers of PS in the newborn salivary DNA. Results: A total of 728 pregnant women were screened for stress exposure using Cohen Perceived Stress Scale (PSS), 164 women were enrolled, and 114 dyads were analyzed. Prenatal Distress Questionnaire (PDQ) was also administered to assess specific pregnancy worries. Transabdominal fetal electrocardiograms (taECG) were recorded to derive coupling between maternal and fetal heart rates resulting in a 'Fetal Stress Index' (FSI). Upon delivery, we collected maternal hair strands for cortisol measurements and newborn's saliva for epigenetic analyses. DNA was extracted from saliva samples, and DNA methylation was measured using EPIC BeadChip array (850 k CpG sites). Linear regression was used to identify associations between PSS/PDQ/FSI/Cortisol and DNA methylation. We found epigenome-wide significant associations for 5 CpG with PDQ and cortisol at FDR < 5%. Three CpGs were annotated to genes (Illumina Gene annotation file): YAP1, TOMM20 and CSMD1, and two CpGs were located approximately lay at 50 kb from SSBP4 and SCAMP1. In addition, two differentiated methylation regions (DMR) related to maternal stress measures PDQ and cortisol were found: DAXX and ARL4D. Conclusions: Genes annotated to these CpGs were found to be involved in secretion and transportation, nuclear signaling, Hippo signaling pathways, apoptosis, intracellular trafficking and neuronal signaling. Moreover, some CpGs are annotated to genes related to autism, post-traumatic stress disorder (PTSD) and schizophrenia. However, our results should be viewed as hypothesis generating until replicated in a larger sample. Early assessment of such noninvasive PS biomarkers will allow timelier detection of babies at risk and a more effective allocation of resources for early
The aim of the study was to evaluate adrenal axis hyperactivation measuring hair cortisol levels,... more The aim of the study was to evaluate adrenal axis hyperactivation measuring hair cortisol levels, and its influence on the relationship among metabolic parameters, inflammation markers and androgens in adult women with PCOS. 44 women (18–34 years) with PCOS diagnosis and a control group of 49 healthy women (19–35 years) were included. In both gropus body mass index (BMI) was calculated and waist circumference (WC) was measured. Hair cortisol, total serum testosterone (TT), serum cortisol, 25 OH vitamin D (25OHD), insulin, high sensitivity C-reactive protein (hsCRP), triglycerides (TG), HDL cholesterol (HDL), glucose and leptin were measured. Bioavailable testosterone (bioT) was calculated. Hair cortisol concentration was higher and significantly different in PCOS patients compared to the control group (130 vs 63 pg/mg of hair, p < 0.001). Subsequently, patients with PCOS were divided into two groups according to hair cortisol levels: group 1 with normal hair cortisol concentratio...
Cortisol is considered one of the most useful parameters to evaluate alterations of the hypothala... more Cortisol is considered one of the most useful parameters to evaluate alterations of the hypothalamic-pituitary-adrenal (HHA) axis. Measurement of serum, salivary and urinary cortisol only provides information of short periods of time, therefore alternative matrices such as hair are proposed as it would reflect cortisol levels of the last 3 months. The aim of this study was to evaluate the association of chronic stress with different pathologies that involve a deregulation of HHA axis through the measurement of hair cortisol by an automated method. The study population consisted of 286 individuals, 232 healthy individuals without medication or adrenal pathology, of whom 213 individuals were healthy and stress-free according to Holmes-Rahe life events scale. In healthy stress-free individuals, median hair cortisol was 55 pg/mg of hair (40-173), whereas in stressed individuals it was 250 pg/mg of hair (182-520). The reference interval was 40-128 pg/mg of hair. In order to assess the cl...
HIV infection is a major risk factor predisposing for Mycobacterium tuberculosis infection and pr... more HIV infection is a major risk factor predisposing for Mycobacterium tuberculosis infection and progression to active tuberculosis (TB). As host immune response defines the course of infection, we aimed to identify immuno-endocrine changes over six-months of anti-TB chemotherapy in HIV+ people. Plasma levels of cortisol, DHEA and DHEA-S, percentages of CD4+ regulatory T cell subsets and number of IFN-γ-secreting cells were determined. Several cytokines, chemokines and C-reactive protein levels were measured. Results were correlated with clinical parameters as predictors of infection resolution and compared to similar data from HIV+ individuals, HIV-infected persons with latent TB infection and healthy donors. Throughout the course of anti-TB/HIV treatment, DHEA and DHEA-S plasma levels raised while cortisol diminished, which correlated to predictive factors of infection resolution. Furthermore, the balance between cortisol and DHEA, together with clinical assessment, may be considere...
In the critical context of COVID-19 pandemic, healthcare workers are on the front line, participa... more In the critical context of COVID-19 pandemic, healthcare workers are on the front line, participating directly in the care, diagnosis, and treatment of patients with COVID-19. This exposes them to a higher risk of developing chronic stress, psychological distress, and any other mental health symptoms. Objective: to evaluate stress and burnout in a health workers population and, in addition, to measure hair cortisol concentration as a current biomarker of stress. Materials and methods: 234 health workers from Hospital de Clínicas "José de San Martín", Buenos Aires University, were included in this study. In this population hair samples were obtained from the posterior vertex as close to the scalp as possible and the individuals completed the following surveys: perceived stress, social support, burnout scale, life event scale, and sociodemographic data. Hair cortisol was measured by an automated chemiluminescent method. The studied population was divided into three groups considering those individuals below the healthy reference sample range (< 40 pg/mg hair), within the healthy reference range (40-128 pg/mg hair) and above the reference range (> 128 pg/mg hair). This study used a transversal and observational design. Results: Our results show that 40% of the studied population presented hair cortisol values outside of the healthy reference range. In the whole studied population, a direct correlation was found between hair cortisol concentration and perceived stress as well as between hair cortisol concentration and the emotional exhaustion component of burnout (r = 0.142, p = 0.030; r = 0.143, p = 0.029, respectively). 12% of the studied population showed Burnout (52% doctors and residents, 19% nurses, 19% administrative personnel). Higher values in hair cortisol levels were found in the group with burnout versus individuals without burnout (p = 0.034). Finally, a mediation analysis was performed, finding that depersonalization is a mediating variable in the relationship between self-perceived stress and hair cortisol level (F = 4.86, p = 0.0086; indirect effect IC: 0.0987-1.8840). Conclusion: This is the first study in which a stress biomarker such as hair cortisol is evaluated in this population and in this context. Healthcare workers are subjected to increased levels of stress and burnout. High depersonalization, emotional exhaustion, and decreased personal sense of accomplishment characterize this population. It is the responsibility of the health authorities to implement strategies to manage this psychological emergency.
American Journal of Clinical and Experimental Medicine, 2019
The diagnostic procedure for the evaluation of Cushing's syndrome is performed by any of the foll... more The diagnostic procedure for the evaluation of Cushing's syndrome is performed by any of the following biochemical tests: urine free cortisol, salivary cortisol at 11 pm and serum cortisol post 1 mg of dexamethasone. Collection of saliva samples is simple and noninvasive, thus being a method of choice for the evaluation of risk populations. The aim of this work is to analyze the performance of an automated chemiluminescent method for measurement of salivary cortisol at 11 pm according to the new quality guidelines and assess its clinical utility. Cortisol levels were measured in samples obtained by passive drooling from 32 healthy subjects and 9 patients with Cushing's syndrome. Matrix effect, linearity, limit of blank, limit of quantitation, recovery and diagnostic performance were assessed. The Unicel 600 DXI Access Beckman Coulter chemiluminescent automated analyzer was used. The standard curve provided by the manufacturer was adapted to measure cortisol concentrations in saliva. Matrix effect: equation of the curve using salivary matrix: y=-1.824x+3.491 (95% CI=-2.068 to-1.582) vs. Equation of the curve using diluent matrix: y=-1.833x+3.394 (95% CI=-1.961 to-1.704). There is overlapping of both curves.
Insulin-like growth factor I (IGF-I) represents an essential tool in the diagnostic work-up and i... more Insulin-like growth factor I (IGF-I) represents an essential tool in the diagnostic work-up and in the monitoring of treatment efficacy for somatotrophic axis disorders both in children and adults. A large number of factors including, but not limited to, age, sex and weight as well as analytical variables influence IGF-I serum levels; therefore, reliable normative data are essential for a correct interpretation of results. The aim of the present study was to establish reference range values for serum IGF-I, in a large population of healthy adults from Buenos Aires city. The study included serum samples from 1044 healthy subjects aged 21-87 years (423 females and 621 males) divided into groups by sex at 5-year intervals from 21 to &gt;75 years. Serum IGF-I concentrations were determined by a fully automated two-site, solid-phase, enzyme-labeled chemiluminescent immunometric assay (Immulite 2000, Siemens Healthcare Diagnostics). IGF-BP interferences are circumvented by blocking IGF-BP binding sites with excess IGF-II in the on-board predilution step. Results show the age dependence of circulating IGF-I levels, with a smooth and steady decrease in levels with age. No sex differences were found in subjects &gt;26 years; however, in the group aged 21-25 years, IGF-I levels were significantly higher in females. In conclusion, this study provides age- and gender-adjusted normal reference ranges for IGF-I levels obtained with an automated immunometric chemiluminescent assay Immulite 2000 in healthy adult subjects.
To evaluate the relationship between testosterone levels and the metabolic syndrome (MS) in men o... more To evaluate the relationship between testosterone levels and the metabolic syndrome (MS) in men older than 45 years. Six hundred and sixty men (45-70 years) selected from 2906 participants of a population screening for prostate cancer were included in this study. Testosterone and the components of MS were assessed in all men. MS was diagnosed according to NCEP-ATP III criteria. Triglycerides (TG)/HDL-cholesterol (chol) index was calculated. The presence of MS was inversely associated with testosterone (χ2, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001), independently of age (OR 0.802, CI 95%: 0.724-0.887, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001). Hypertension was the most frequent abnormality observed followed by elevated TG and waist circumference (WC). Testosterone correlated positively with HDL-chol (r: 0.14, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001) and negatively with body mass index (BMI)(r: -0.29, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), WC (r: -0.26, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), TG (r: -0.20, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), TG/HDL-chol (r: -0.20, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), glucose (r: -0.11, p = 0.005) and MS score (r: -0.23, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001). Our results show that in men older than 45 years, as long as testosterone levels decline, the prevalence of MS increases, independently of age. The correlations found between testosterone and four of the five components of MS, as well as with BMI and TG/HDL-chol ratio, a surrogate marker of insulin resistance, suggest considering male hypogonadism as a determinant of developmental abnormalities typical of MS.
Introducción: El cáncer de próstata (CaP) es la tercera causa de muerte oncológica en el varón. E... more Introducción: El cáncer de próstata (CaP) es la tercera causa de muerte oncológica en el varón. En el paciente obeso hay múltiples alteraciones hormonales que se podrían relacionar con el aumento de la incidencia del CaP. Objetivo: Estudiar la relación entre el perfil lipoproteico y la obesidad y su relación como factores asociados con el CaP. Materiales y Métodos: Se seleccionaron 150 pacientes de entre 50 y 65 años: 50 con CaP, 50 con Hiperplasia Prostática Benigna (HPB) y 50 sin patología prostática. Se les realizó un dosaje de Antígeno Prostático Específico total (PSAt) y Examen Digital Rectal (EDR). Resultados: Los pacientes con CaP presentaron valores de PSAt mayores que las otras dos poblaciones. No hubo diferencias en el Índice de Masa Corporal (IMC) entre los tres grupos. Los pacientes con CaP presentaron valores disminuidos de Colesterol-HDL y una relación Triglicéridos/Colesterol-HDL más alta. Los pacientes con HPB presentaron menores valores de Colesterol-total con respecto a los controles, sin diferencias con respecto a los pacientes con CaP. Se encontraron correlaciones inversas significativas entre el PSAt y el Colesterol-total y el Colesterol-HDL. Conclusiones: El descenso de colesterol-total en los pacientes con CaP se atribuiría al descenso de colesterol-HDL, con el consiguiente aumento de la relación triglicéridos/col-HDL, la cual sería un marcador de insulino-resistencia.
To evaluate lipoprotein profile and sex hormones in patients with prostate cancer (PCa) and benig... more To evaluate lipoprotein profile and sex hormones in patients with prostate cancer (PCa) and benign prostatic hyperplasia (BPH) and their possible associations with some inflammatory markers linked to PCa. A total of 150 men (50-65 years), matched by age and body mass index (BMI), included in this study and divided into three groups according to total prostate specific antigen (PSA), digital rectal examination and prostate biopsy: 50 PCa, 50 BPH and 50 controls. Total cholesterol (Chol), HDL-chol, LDL-chol, triglycerides (TG), total testosterone (T), free T (FT), bioavailable T (BioT), estradiol and SHBG were measured. The free androgen index (FAI) and TG/HDL-chol were calculated. In 25 PCa and 25 controls, C-reactive protein (hs-CRP), adiponectin and insulin were determined. Patients with PCa showed higher TG/HDL-chol and diminished HDL-chol than Controls and BPH. PSA correlated inversely with HDL-chol and directly with TG/HDL-chol. FAI, FT, BioT and estradiol levels were higher, and SHBG and adiponectin were lower in PCa than in Controls. No differences were found in androgens between BPH and PCa. Our most novel findings are that the patients with PCa presented lower total Chol and HDL-chol and higher TG/HDL-chol than BPH and Controls. Patients with PCa showed higher androgens and lower adiponectin than Controls.
Objectives — The objective of our study was to assess humoral response in a population of health ... more Objectives — The objective of our study was to assess humoral response in a population of health workers after vaccination with the first and second doses of Sputnik V. Methods — SARS-CoV-2 total antibodies (IgG and IgM) were measured, using the Centaur XPT autoanalyzer, Siemens®, in 530 serum samples taken from health workers in Buenos Aires vaccinated with Sputnik V. Results — After 21 days of the first dose application, 10 individuals (1.9%) presented antibody levels <1.0 (non-reactive), while 520 subjects (98.1%) responded with antibody values >1.0 (reactive). The results, obtained 21 days after the second dose, show that only 2 individuals (0.38%) had antibody levels <1.0 (non-reactive) and 528 (99.6%) responded with antibody values >1.0 (reactive). Conclusion — This study results implied that two doses of Sputnik V vaccine generated a proper antibody response in virtually the entire studied population.
The adverse effects of maternal prenatal stress (PS) on child’s neurodevelopment warrant the esta... more The adverse effects of maternal prenatal stress (PS) on child’s neurodevelopment warrant the establishment of biomarkers that enable early interventional therapeutic strategies. We performed a prospective matched double cohort study screening 2000 pregnant women in third trimester with Cohen Perceived Stress Scale-10 (PSS-10) questionnaire; 164 participants were recruited and classified as stressed and control group (SG, CG). Fetal cord blood iron parameters were measured at birth. Transabdominal electrocardiograms-based Fetal Stress Index (FSI) was derived. We investigated sex contribution to group differences and conducted causal inference analyses to assess the total effect of PS exposure on iron homeostasis using a directed acyclic graph (DAG) approach. Differences are reported for p<0.05 unless noted otherwise. Transferrin saturation was lower in male stressed neonates. The minimum adjustment set of the DAG to estimate the total effect of PS exposure on fetal ferritin iron b...
In the pregnant mother and her fetus, chronic prenatal stress results in entrainment of the fetal... more In the pregnant mother and her fetus, chronic prenatal stress results in entrainment of the fetal heartbeat by the maternal heartbeat, quantified by the fetal stress index (FSI). Deep learning (DL) is capable of pattern detection in complex medical data with high accuracy in noisy real-life environments, but little is known about DL’s utility in non-invasive biometric monitoring during pregnancy. A recently established self-supervised learning (SSL) approach to DL provides emotional recognition from electrocardiogram (ECG). We hypothesized that SSL will identify chronically stressed mother-fetus dyads from the raw maternal abdominal electrocardiograms (aECG), containing fetal and maternal ECG. Chronically stressed mothers and controls matched at enrolment at 32 weeks of gestation were studied. We validated the chronic stress exposure by psychological inventory, maternal hair cortisol and FSI. We tested two variants of SSL architecture, one trained on the generic ECG features for emo...
Revista de la Sociedad Argentina de Diabetes, 2020
Introducción: la vía inflamatoria JNK (c-Jun N-terminal Kinase) desempeña un rol central en el de... more Introducción: la vía inflamatoria JNK (c-Jun N-terminal Kinase) desempeña un rol central en el desarrollo de neuroinflamación y diabetes mellitus tipo 2 (DM2). La inflamación subclínica es una característica emblemática de DM2 y entidades asociadas. Se acompaña de activación de macrófagos que, de manera ubicua, infiltran y dañan una miríada de órganos, incluido SNC y páncreas. Dichos macrófagos descienden de monocitos inflamatorios que sobreexpresan, precozmente, la vía JNK, incluso en personas no diabéticas, con riesgo de DM2. Aunque se conoce que obesidad y DM2 afectan el SNC, dicha asociación es compleja. Estudios longitudinales demostraron que un elevado coeficiente intelectual, en niños, se asocia a menor obesidad en la adultez a partir de un estilo de vida saludable, sugiriendo que el eje diabesidad-SNC sería bidireccional.Objetivos: explorar interrelaciones entre la expresión monocitaria de JNK, la velocidad de reacción psicomotora (medida correlacionada con la inteligencia) ...
In a pregnant mother and her fetus, chronic prenatal stress results in entrainment of the fetal h... more In a pregnant mother and her fetus, chronic prenatal stress results in entrainment of the fetal heartbeat by the maternal heartbeat, quantified by the fetal stress index (FSI). Deep learning (DL) is capable of pattern detection in complex medical data with high accuracy in noisy real-life environments, but little is known about the utility of DL in non-invasive biometrics during pregnancy. We hypothesized that a recently established self-supervised learning (SSL) approach that provides emotional recognition from ECG will identify chronically stressed mother-fetus dyads from the raw maternal abdominal electrocardiograms (aECG), containing fetal and maternal ECG. Chronically stressed mothers and controls matched on enrolment at 32 weeks of gestation were studied. We validated the chronic stress exposure by psychological inventory, maternal hair cortisol, and FSI. We tested two variants of SSL architecture, one trained on the generic ECG features for emotional recognition obtained from...
Asian Pacific journal of cancer prevention : APJCP, 2016
Prostate cancer (PCa) is one of the most common diseases in men. It is important to assess progno... more Prostate cancer (PCa) is one of the most common diseases in men. It is important to assess prognostic factors and whether high cortisol levels and complex hormonal interactions could be responsible for PCa development. We evaluated the relationship between cortisol, leptin and estrogens in 141 men, 71 with PCa and the remaining 70 constituting a low risk group (LRG). They were recruited for this study from a total of 2906 middleaged men (ages 4570 years) who completed an evaluation for prostatic diseases at the Urology Division, Hospital de Clinicas "Jose de San Martin", University of Buenos Aires, in May 2009. In this cross sectional study, cortisol, PSA, totaltestosterone, freetestosterone, bioavailable testosterone, LH and estradiol were measured in serum. We observed increased cortisol levels in PCa patients as compared to LRG cases (p=0.004,). Leptin and estradiol levels were also higher in PCa patients (p=0.048; p<0.0001, respectively). Logistic regression analysi...
Revista de la Sociedad Argentina de Diabetes, 2020
Introducción: la depresión (DP) tiene una alta prevalencia en pacientes con diabetes mellitus tip... more Introducción: la depresión (DP) tiene una alta prevalencia en pacientes con diabetes mellitus tipo 1 (DM1) y se asocia a repercusiones clínicas negativas como mayor morbimortalidad cardiovascular y complicaciones crónicas. Existen pocos estudios publicados sobre la funcionalidad del eje hipotálamo-hipófiso-adrenal (H-H-A) en DM1 con DP, y la relación entre la DP y el test de respuesta del cortisol al despertar (RCD) con el control glucémico (CG).Objetivos: analizar la funcionalidad del eje H-H-A a través de la evaluación del RCD en pacientes con DM1 (PD1) con y sin DP. Como objetivos secundarios, conocer la prevalencia de DP en PD1 y ver si existe relación entre el RCD y CG y entre DP y CG.Materiales y métodos: estudio observacional, prospectivo, de corte transversal, multicéntrico, nacional. Se incluyeron PD1 mayores de 18 años; se utilizó cuestionario Patient Health Questionnaire-9 (PHQ-9) para diagnóstico de DP. Se tomaron muestras de cortisol salival al despertar y a los 30 minu...
Introduccion: La hiperplasia prostatica benigna (HPB) constituye una patologia con alta prevalenc... more Introduccion: La hiperplasia prostatica benigna (HPB) constituye una patologia con alta prevalencia en hombres mayores, y en su patogenia es posible la influencia de la inflamacion prostatica. La resistencia a la insulina, que se presenta con hiperinsulinemia (que forma parte del sindrome metabolico [SM]) y el factor de crecimiento simil insulina tipo 1 (insulin-like growth factor-1, IGF-1) (relacionado con la obesidad) se asocian al aumento de sintomas urinarios por inflamacion prostatica y crecimiento prostatico debido a que influyen sobre vias de senalizacion que generan proliferacion celular. A su vez, la leptina (tambien elevada en la obesidad) poseeria efectos sobre el crecimiento celular. Objetivo: Evaluar la relacion entre estas tres moleculas (resistencia a la insulina, IGF-1 y leptina) con la hiperplasia prostatica benigna. Material y metodo: Poblacion seleccionada de una convocatoria realizada bajo la denominacion “Campana de deteccion temprana del cancer de prostata”. Se...
Uploads
Papers by Bibiana Fabre