Papers by Benito de Brito
Revista Brasileira de Ciência Avícola, 2016
Brazilian Journal of Microbiology, 2021
In this article the wrong figure appeared as Fig. 1.; the figure should have appeared as shown be... more In this article the wrong figure appeared as Fig. 1.; the figure should have appeared as shown below. Publisher's note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Medicina: Progresso científico, tecnológico, econômico e social do país 2, 2021
The skin lesions in broilers are responsible for great losses at the slaughter. Lesions which hav... more The skin lesions in broilers are responsible for great losses at the slaughter. Lesions which have no specific classification are categorized as dermatosis in post-mortem examination. The aim of this study was to identify the main variables associated with the presentation of dermatosis through epidemiological study addressing management issues, health and ambience throughout the production period. Data were analyzed by logistic regression, using the score for model selection through the LOGISTIC procedure of SAS. It was identified a set of variables associated with the manifestation of this skin diseases in broilers. The prevalence of dermatosis was 1.31%. The variables whose presence significantly increased the expression of these lesions were: the chicks housed over reused poultry litter, not disinfecting equipment before the housing, reducing the interval between batches, and the use of tubular feeders. Commercial broiler lines and sex showed a great effect on the presence of de...
Pesquisa Agropecuária Gaúcha, 2018
A patogenia de Salmonella em peixes é desconhecida e diversas são as formas de contaminação do pe... more A patogenia de Salmonella em peixes é desconhecida e diversas são as formas de contaminação do pescado por este microrganismo, sem relatos que esclareçam sua função como agente etiológico ou como pertencente à microbiota destes animais. Em diversos países esta bactéria é responsável por milhões de casos de Salmonelose e as salmonelas paratíficas são as principais causadoras de doenças associadas ao consumo de peixes e crustáceos. Vários fatores podem influenciar na segurança dos alimentos da aquicultura, como o tipo das espécies cultivadas, localização dos criadouros, práticas de cultivo e manejo, preparo e consumo do pescado. Além disso, a escassez de saneamento básico ou disposição de rejeitos animais e humanos de forma inapropriada contribuem para a poluição desses ambientes. A contaminação do ecossistema aquático por Salmonella torna o ambiente uma fonte de disseminação desta bactéria. A crescente expansão da aquicultura como fonte de alimentos reforça a importância de um sistem...
Frontiers in Microbiology, 2021
This study discussed the use of antimicrobials in the commercial chicken production system and th... more This study discussed the use of antimicrobials in the commercial chicken production system and the possible factors influencing the presence of Extended-spectrum β-lactamase (ESBL)/AmpC producers strains in the broiler production chain. The aim of this study was to perform longitudinal monitoring of ESBL-producing and fosfomycin-resistant Escherichia coli from poultry farms in southern Brazil (Paraná and Rio Grande do Sul states) and determine the possible critical points that may be reservoirs for these strains. Samples of poultry litter, cloacal swabs, poultry feed, water, and beetles (Alphitobius sp.) were collected during three distinct samplings. Phenotypic and genotypic tests were performed for characterization of antimicrobial resistant strains. A total of 117 strains were isolated and 78 (66%) were positive for ESBL production. The poultry litter presented ESBL positive strains in all three sampled periods, whereas the cloacal swab presented positive strains only from the se...
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2016
Salmonella Enteritidis and Salmonella Typhimurium are responsible for causing huge economic loses... more Salmonella Enteritidis and Salmonella Typhimurium are responsible for causing huge economic loses in aviculture, as they lead young broiler chicks to develop clinical disease and thus increase mortality. Salmonella's pathogenicity is considered complex and multifactorial, demanding more studies that could elucidate the interaction between host and pathogen. The present study aims to evaluate the virulence of 130S. Enteritidis isolates and 70S. Typhimurium inoculated in one-day-old chicks through the establishment of a pathogenicity index. For each strain, 10 commercial chicks from the Cobb lineage were used. Then, 200µL of a solution containing 2x108 CFU of S. Enteritidis or S. Typhimurium were inoculated in the birds by intraperitoneal via. Mortality and presence of lesions such as aerosaculitis (A), perihepatitis (Ph), pericarditis (Pc), peritonitis (Pt), onfalitis (O) and cellulitis (C) were registered daily for seven days. From the second to the seventh day there was a propo...
Semina: Ciências Agrárias, 1996
Veterinary Microbiology, 2003
Colibacillosis caused by Escherichia coli is one of the most common diseases in commercial broile... more Colibacillosis caused by Escherichia coli is one of the most common diseases in commercial broiler chickens, which is associated with a significant financial loss in the poultry industry due to morbidity, mortality and high costs of antibiotic overuse. Diseases caused by E. coli mostly present as secondary or opportunistic pathogens, while certain strains of these bacteria with specific virulence factors could act as primary pathogens. This study aimed to detect three virulence genes and determine antibiotic resistance profiles in the E. coli isolates of commercial broilers with colibacillosis in Tabriz, Iran using multiplex polymerase chain reaction (PCR) and disc-diffusion method, respectively. According to the results, out of 71 bacterial isolates, 38 (53.5%), 25 (35.2%) and 35 cases (49.3%) carried the iron-repressible protein (irp2), pyelonephritis-associated pili (papC) and temperature-sensitive hemagglutinin (tsh) genes, respectively. Moreover, 16 isolates contained the irp2 gene only, eight isolates had the papC gene only, 13 isolates carried the tsh gene only, and 12 isolates lacked all the three genes. In 39 isolates, 2-3 genes were identified simultaneously. In this study, most of the isolates were resistant to enrofloxacin, doxycycline, sultrim, erythromycin and danofloxacin, while they were sensitive to colistin only. Frequency of the isolates susceptible to fosfomycin, neomycin and florfenicol was 45.9%, 45.2% and 48.3%, respectively. Therefore, it could be concluded that high rate of antibiotic resistance and prevalence of some important virulence genes in avian pathogenic E. coli isolates might be a serious hazard for the poultry industry and public health.
Arquivos de Ciências Veterinárias e Zoologia da UNIPAR, 2015
Os principais hospedeiros do Metapneumovírus aviário (aMPV) são os frangos de corte e perus. O ví... more Os principais hospedeiros do Metapneumovírus aviário (aMPV) são os frangos de corte e perus. O vírus acomete o trato respiratório superior dos perus desencadeando a Rinotraqueíte Viral dos Perus (RVP). O principal objetivo deste trabalho foi padronizar uma técnica de RT-PCR para a detecção do aMPV, por meio do uso do kit AccessQuick™ RT-PCR system (Promega®). Foram utilizados amostras de suabes de traqueia e pulmão de 38 perus comerciais com sintomatologia respiratória e dois suabes oculares de faisão. O RNA viral foi extraído utilizando-se o kit RTP® DNA/RNA Virus Mini Kit (STRATEC Molecular). Em seguida as amostras foram submetidas à RT-PCR One Step, utilizando o kit AccessQuick™ RT-PCR system (Promega®). Todas as 40 amostras testadas por RT-PCR foram negativas, exceto a amostra vacinal que foi utilizada como controle positivo. O aMPV não causa latência em frangos de corte ou perus, logo a excreção viral é limitada. Dessa forma, a ausência da detecção de genoma viral neste estudo ...
Salmonella Enteritidis and Salmonella Typhimurium are responsible for causing huge economic loses... more Salmonella Enteritidis and Salmonella Typhimurium are responsible for causing huge economic loses in aviculture, as they lead young broiler chicks to develop clinical disease and thus increase mortality. Salmonella's pathogenicity is considered complex and multifactorial, demanding more studies that could elucidate the interaction between host and pathogen. The present study aims to evaluate the virulence of 130S. Enteritidis isolates and 70S. Typhimurium inoculated in one-day-old chicks through the establishment of a pathogenicity index. For each strain, 10 commercial chicks from the Cobb lineage were used. Then, 200µL of a solution containing 2x10 8 CFU of S. Enteritidis or S. Typhimurium were inoculated in the birds by intraperitoneal via. Mortality and presence of lesions such as aerosaculitis (A), perihepatitis (Ph), pericarditis (Pc), peritonitis (Pt), onfalitis (O) and cellulitis (C) were registered daily for seven days. From the second to the seventh day there was a prop...
Microbiological contamination in commercial poultry production has caused concerns for human heal... more Microbiological contamination in commercial poultry production has caused concerns for human health because of both the presence of pathogenic microorganisms and the increase in antimicrobial resistance in bacterial strains that can cause treatment failure of human infections. The aim of our study was to analyze the profile of antimicrobial resistance and virulence factors of E. coli isolates from chicken carcasses obtained from different farming systems (conventional and free-range poultry). A total of 156 E. coli strains were isolated and characterized for genes encoding virulence factors described in extraintestinal pathogenic E. coli (ExPEC). Antimicrobial susceptibility testing was performed for 15 antimicrobials, and strains were confirmed as extended spectrum of -lactamases-(ESBLs-) producing E. coli by phenotypic and genotypic tests. The results indicated that strains from freerange poultry have fewer virulence factors than strains from conventional poultry. Strains from con...
Comparative Immunology, Microbiology and Infectious Diseases
Research, Society and Development
Na busca de técnicas que possam auxiliar na redução de micro-organismos que podem estar presentes... more Na busca de técnicas que possam auxiliar na redução de micro-organismos que podem estar presentes durante o abate, pesquisas estão sendo feitas com o uso dos ácidos orgânicos e óleos essenciais como sanitizante das carcaças. O objetivo do presente trabalho foi avaliar a influência do jejum pré-abate de frangos de corte na perda de peso e contaminação por Salmonella spp. do papo e eficácia do banho das carcaças com um blend de ácidos orgânicos e óleo essencial na redução de micro-organismos indicadores de contaminação. Foram avaliados três lotes de frangos de corte de uma integração, sendo que somente em dois lotes foi avaliado a perda de peso após o período de jejum. De cada lote foram colhidas amostras de 40 aves. O produto utilizado foi diluído nas concentrações de 4, 8 e 12%. A análise da presença de micro-organismos indicadores de contaminação foi feita: (1) antes da toalete final; (2) após a toalete final e (3) após o chiller. Verificou-se que a perda de peso variou entre os do...
Arquivos do Instituto Biológico
ABSTRACT: The use of antimicrobials in fish farming is a reflection of the fast aquaculture devel... more ABSTRACT: The use of antimicrobials in fish farming is a reflection of the fast aquaculture development worldwide. The intensification of aquaculture to achieve market demands could lead to an increase in infectious diseases by pathogenic bacteria. Consequently, antimicrobials act as controls for emerging infectious diseases, but their use must follow the rules and regulations of the country where the activity is performed. Although the regulations impose limits to the use of antimicrobials in fish farming, many studies show that resistant bacteria are isolated from this system. The selection of resistant bacteria is not limited only to the use of antimicrobials, but also to co-selection of resistance genes or even with cross-resistance processes. Resistant bacteria from fish farming are a serious concern because they can be acquired by humans with handling or food chain, which may represent a public health problem. In the present review, we present an overview of antimicrobials use...
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Papers by Benito de Brito