Papers by Bambang Hariono
Manusia Dan Lingkungan, 1998
Journal of Biota, Feb 1, 2012
Lead is toxic to human. Green tea has been used to treat many kind of disease because of catechin... more Lead is toxic to human. Green tea has been used to treat many kind of disease because of catechin. The objective of the studies were to know effect of catechin in green tea extract as a chelating agent of Pb. Thirty six male Wistar rat were used in this studies. Rats were devided into 6 groups of 6. First group as a negative control. The rest was the treatment groups that was given Pb acetate for 66 days. The second group as positive control. At the 35 th day of studies group III, IV, and V were given green tea extract at 0 g/kg bodyweight, 0.75 g/kg bodyweight, 1.5 g/kgbodyweight, 3 g/kg bodyweight respectively. The group VI was given dimercaprol as a standard clinical medicine. Parameter recorded were mallondialdehide, gluthation peroxidase activity and nitric oxide that measured by commercial kits. The different among treatments were analyzed using ANOVA, followed by Duncan to locate different. The result showed that all dosis of green tea extract could decrease MDA, blood lead and could increase serum GPx and NO. The optimum dosis was at 3 g/kg bodyweigt that could decrease MDA for 2.75 µ mol/L to 0.07 µ mol/L and blood lead for 2.35 ppm to 0.02 ppm and increased serum GPx for 58.5 µ mol/L to 177 µ mol/l, serum NO for 1,2 µ mol/L to 7.95 µmol/L. It could be concluded that green tea extract could be used as a Pb chelator.
Jurnal Sain Veteriner, 2009
Twenty male rats (Rattus norvegicus) everage body weight of 200 g were devided into five groups. ... more Twenty male rats (Rattus norvegicus) everage body weight of 200 g were devided into five groups. Group I as control rats and the other groups (II, III, IV and V) as treated rats. All treated rats were given 0.125mL/200 g bw ofCC14 orally for ten times (day-l until day-19 with 2 days interval) and the control group were given 0.125 mL/200 g bw aquadest. Since day-21 until day-35, only group IV and V were given 0.56 mL buah merah extract every day. Rats group I, II, and III were given 0.56 mL aquadest every day since day-21. Each rat of group III and V were killed at day-21, 23, 27 and 35, then a part ofliver tissue were collected for histopathological examination. The research showed that CC14 administration with dose of 0.125 mL/200 g bw for ten times with 2 days interval caused significantly increase of ALT activity compared to control group. At day-35 showedALT activity of treated group II and IV were declined compared to day-21. Histopathological examination showed that hepatic congestion, radiary structure of hepatocytes was not clear, hydropic degeneration, and central lobular necrosis. Liver regeneration process of the treated which were given buah merah extract appeared faster then the treated rat which were given CC14only.
11 Jurnal Manusia Dan Lingkungan, Jul 5, 2013
American Journal of Immunology, 2015
Jembrana disease is a bovine disease that affects Bali cattle (Bos javanicus). The causal agent i... more Jembrana disease is a bovine disease that affects Bali cattle (Bos javanicus). The causal agent is named Jembrana Disease Virus (JDV), a lentivirus member. The disease development of Jembrana disease in Bali cattle is unique for a lentivirus infection as it is related with severe clinical syndrome in an acute period. In experimentally JDV-infected Bali cattle, the death rate was about 21% and occurred within only 1 to 2 weeks postinfection. Indeed, the mortality of more than 60 000 cattles in a year was observed during the first outbreak and the disease is now endemic throughout parts of Indonesia. Early diagnosis constitutes a preventive method of further disease outbreaks. The Jembrana disease can be diagnosed by the clinical symptoms but more reliable diagnostic tools are available, based on either antigen (immunodiagnosis) or viral genome (molecular diagnosis). In this review, we summarize about immunodiagnostic tools of Jembrana disease which has been developed so far.
Jurnal Sain Veteriner, Mar 20, 2012
Uploads
Papers by Bambang Hariono