Journal of the Indian Society of Remote Sensing, 1975
A systematic air-photo-interpretation procedure employing physiographic analysis ~aas been used i... more A systematic air-photo-interpretation procedure employing physiographic analysis ~aas been used in preparing a quick inventory of the problem areas of parts of Hoshiarpur district of Punjab.
The self configuring dexterous autonomy of MANET imposes some network challenges constrained to t... more The self configuring dexterous autonomy of MANET imposes some network challenges constrained to traditional dynamic routing behaviour. So as working with different mobility and traffic patterns with normal management schemes may lead some minor pitfalls to some important network performance parameters and hence can degrade the whole network performance. Here, Our aim is to make some DSR and MAC based cross layer optimizations and testify it on different mobility and traffic scenarios so as to justify the robustness of our proposed improvement.
Studies were carried out on in-vitro induction of apoptosis in avian lymphocyte due to zinc phosp... more Studies were carried out on in-vitro induction of apoptosis in avian lymphocyte due to zinc phosphide, a rodenticide. For this purpose one batch of avian splenic lymphocytes were treated with various concentrations of NOEL/102, NOEL/103, NOEL/104, NOEL/105, NOEL/106 and NOEL/107 dose of zinc phosphide for 30, 60 and 90 min and another batch of cells was kept as untreated control. The cells were collected in 2.5% glutaraldehyde for ultrastructural examination on transmission and scanning electron microscope. Immunoperoxidase staining was performed for demonstrate of phosphatidylserine using Annexin-V biotin and avidin peroxidase conjugate. Agarose gel electrophoresis was carried out on isolated DNA for ladder pattern and lymphocyte proliferation assay was performed in 96 well sterilized culture plates using ConA and LPS as mitogen for T- and B-Iymphocytes, respectively. It can be concluded from the present study that the zinc phosphide can induce the apoptosis in avian lymphocytes which may further lead to a state of immunosuppression. It constitutes the first report of zinc phosphide induced apoptosis in avian lymphocytes.
The present investigation aims at evaluating the carbendazim for deleterious effects and inductio... more The present investigation aims at evaluating the carbendazim for deleterious effects and induction of apoptosis in avian lymphocytes. For this purpose one batch of splenic avian lymphocytes was treated with various concentrations (NOEL/102, NOEL/103, NOEL/104, NOEL/105, NOEL/106 and NOEL/107) of carbendazim for 30, 60 and 90 min and another batch of cells was kept as untreated control. The cells were collected for detection of ultrastructural alterations by transmission and scanning electron microscopy. Immunoperoxidase staining was performed for demonstration of phosphatidylserine translocation using Annexin-V biotin conjugate. DNA was isolated from treated and control cells and agarose gel electrophoresis was performed for DNA ladder pattern. Lymphocyte proliferation assay was conducted to assess the effect of carbendazim on T- and B-cell blastogenesis. In carbendazim treated cells, ultrastructural changes included pyknosis, karyorrhexis of chromatin material which marginated on nuclear membrane and shrinkage of cell. The SEM findings revealed the formation of blebs of various size and shape on the surface of cells. The translocation of phosphatidylserine was demonstrated on cell membrane using immunoperoxidase technique. Ladder pattern was observed in agarose gel electrophoresis with smearing of oligonucleotides in comparison to controls and standard DNA markers, which is highly suggestive of apoptosis in avian lymphocytes due to carbendazim exposure. It can be concluded from the present study that the carbendazim induces apoptosis in avian lymphocytes which constitutes the first report of its kind.
As because of modern emerging technologies, low power integrated circuits and wireless communicat... more As because of modern emerging technologies, low power integrated circuits and wireless communication has enabled a new generation of sensors network. The incorporation of these sensors networks in Health care is very popular and plays a vital role in breath breaking situations. The deployment of monitoring hardware incorporated with various wireless standards plays a key role in regard to interoperability, invasion privacy, sensors validation data consistency and interference related issues. The goal of our paper is to make a comparative study in realm of modern wireless trends such as Bluetooth, Wi-fi, Zigbee and Wibree and related facets.
The self configuring dexterous autonomy of MANET imposes some network challenges constrained to t... more The self configuring dexterous autonomy of MANET imposes some network challenges constrained to traditional dynamic routing behaviour. So as working with different mobility and traffic patterns with normal management schemes may lead some minor pitfalls to some important network performance parameters and hence can degrade the whole network performance. Here, Our aim is to make some DSR and MAC based cross layer optimizations and testify it on different mobility and traffic scenarios so as to justify the robustness of our proposed improvement.
WRKY proteins are a large family of plant-specific transcription factors associated with regulati... more WRKY proteins are a large family of plant-specific transcription factors associated with regulation of biotic and abiotic stress responses, but how they respond to cereal rust pathogens has never been explored at the molecular level. Full-length cDNA of TaWRKY1B was obtained from a wheat cultivar HD2329 derivative containing leaf rust resistance gene Lr28 based on domain characteristics. The unique feature of this WRKY transcription factor gene was the close proximity of the DNA-binding domain and consensus DNA element W-Box within the open reading frame. Infection with a virulent race of leaf rust fungus resulted in 146-fold induction of the gene in resistant plants, but only 12-fold in the susceptible plants as compared with mock-inoculated controls. Docking models of 74 amino acids DNA-binding domain and 26 bp W-Box element showed that the WRKY domain, located on the β1 strand, only interacts with the W-Box at positions corresponding to W125, R126, K127 and Y128 amino acids. A tr...
A Wireless Sensor Network(WSN) contains short range energy limited terminals/nodes in which multi... more A Wireless Sensor Network(WSN) contains short range energy limited terminals/nodes in which multiple nodes participates one by one to transfer data from source node to Base station. Each node appends some amount of delay which degrades the network performance as the jittered behaviour of network may not be allowed in some applications. Again the faulty nature of tandem nodes may create some severe routing scenarios. Concept of Broad Area Sensor Network (BASN) incorporates a real time communication between sensor node and Base station, which helps to optimize the Quality of Service (QoS). Our goal is to do extensive simulation on performance analysis of such sensor networks and its optimization.
ISPRS - International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences, 2014
Uttarakhand State of India suffered a widespread devastation in June 2013 due to floods caused by... more Uttarakhand State of India suffered a widespread devastation in June 2013 due to floods caused by excessive rain in the upper reaches of the Himalaya, glacial lake outburst flood (GLOF) and landslides. Restoration process in this mountainous State calls for scientifically sound planning so that the vulnerabilities and risks to such natural hazards are minimised and developmental processes are sustainable in long run. Towards this, an understanding of the patterns and major controls of damage of the recent disaster is a key requirement which can be achieved only if the primary data on locations and types of damage along with other local site conditions are available. Considering widespread damage, tough nature of terrain and the need for collecting the primary data on damage in shortest possible time, crowdsourcing approach was considered to be the most viable solution. Accordingly, a multiinstitutional initiative called "Map the Neighbourhood in Uttarakhand" (MANU) was con...
Defect depth profile study has been carried out in organic semiconductor (OSC) multilayers to cha... more Defect depth profile study has been carried out in organic semiconductor (OSC) multilayers to characterize the buried interfaces and layers using beam based positron annihilation spectroscopy. The bilayer and trilayer heterostructures (p-n, p-p and n-p-n) comprise of organic-organic and organic-inorganic (substrate) interfaces. Our study reveals the presence of defects at the interfaces whose concentration is seen to vary with the layer thickness. The S-W correlation has been used to examine the effect of organic materials as well as thickness of the layers on the defect microstructure in multilayers. The nature and type of defects in p-p bilayer are seen to be different as compared to p-n and n-p-n multilayers. Positron mobility in OSC layers has been calculated from the fitted diffusion length which is seen to be of the same order as the effective mobility of charge carrier obtained from the measured current density-voltage (J-V) characteristics. The role of structural defects and the intrinsic electric field at the interfaces on positron systematics is also examined. Positron diffusion modeling together with experimental data suggests that the defect at the interfaces has a stronger influence on the positron systematics than the intrinsic electric field across organic-organic interfaces.
Journal of the Indian Society of Remote Sensing, 1975
A systematic air-photo-interpretation procedure employing physiographic analysis ~aas been used i... more A systematic air-photo-interpretation procedure employing physiographic analysis ~aas been used in preparing a quick inventory of the problem areas of parts of Hoshiarpur district of Punjab.
The self configuring dexterous autonomy of MANET imposes some network challenges constrained to t... more The self configuring dexterous autonomy of MANET imposes some network challenges constrained to traditional dynamic routing behaviour. So as working with different mobility and traffic patterns with normal management schemes may lead some minor pitfalls to some important network performance parameters and hence can degrade the whole network performance. Here, Our aim is to make some DSR and MAC based cross layer optimizations and testify it on different mobility and traffic scenarios so as to justify the robustness of our proposed improvement.
Studies were carried out on in-vitro induction of apoptosis in avian lymphocyte due to zinc phosp... more Studies were carried out on in-vitro induction of apoptosis in avian lymphocyte due to zinc phosphide, a rodenticide. For this purpose one batch of avian splenic lymphocytes were treated with various concentrations of NOEL/102, NOEL/103, NOEL/104, NOEL/105, NOEL/106 and NOEL/107 dose of zinc phosphide for 30, 60 and 90 min and another batch of cells was kept as untreated control. The cells were collected in 2.5% glutaraldehyde for ultrastructural examination on transmission and scanning electron microscope. Immunoperoxidase staining was performed for demonstrate of phosphatidylserine using Annexin-V biotin and avidin peroxidase conjugate. Agarose gel electrophoresis was carried out on isolated DNA for ladder pattern and lymphocyte proliferation assay was performed in 96 well sterilized culture plates using ConA and LPS as mitogen for T- and B-Iymphocytes, respectively. It can be concluded from the present study that the zinc phosphide can induce the apoptosis in avian lymphocytes which may further lead to a state of immunosuppression. It constitutes the first report of zinc phosphide induced apoptosis in avian lymphocytes.
The present investigation aims at evaluating the carbendazim for deleterious effects and inductio... more The present investigation aims at evaluating the carbendazim for deleterious effects and induction of apoptosis in avian lymphocytes. For this purpose one batch of splenic avian lymphocytes was treated with various concentrations (NOEL/102, NOEL/103, NOEL/104, NOEL/105, NOEL/106 and NOEL/107) of carbendazim for 30, 60 and 90 min and another batch of cells was kept as untreated control. The cells were collected for detection of ultrastructural alterations by transmission and scanning electron microscopy. Immunoperoxidase staining was performed for demonstration of phosphatidylserine translocation using Annexin-V biotin conjugate. DNA was isolated from treated and control cells and agarose gel electrophoresis was performed for DNA ladder pattern. Lymphocyte proliferation assay was conducted to assess the effect of carbendazim on T- and B-cell blastogenesis. In carbendazim treated cells, ultrastructural changes included pyknosis, karyorrhexis of chromatin material which marginated on nuclear membrane and shrinkage of cell. The SEM findings revealed the formation of blebs of various size and shape on the surface of cells. The translocation of phosphatidylserine was demonstrated on cell membrane using immunoperoxidase technique. Ladder pattern was observed in agarose gel electrophoresis with smearing of oligonucleotides in comparison to controls and standard DNA markers, which is highly suggestive of apoptosis in avian lymphocytes due to carbendazim exposure. It can be concluded from the present study that the carbendazim induces apoptosis in avian lymphocytes which constitutes the first report of its kind.
As because of modern emerging technologies, low power integrated circuits and wireless communicat... more As because of modern emerging technologies, low power integrated circuits and wireless communication has enabled a new generation of sensors network. The incorporation of these sensors networks in Health care is very popular and plays a vital role in breath breaking situations. The deployment of monitoring hardware incorporated with various wireless standards plays a key role in regard to interoperability, invasion privacy, sensors validation data consistency and interference related issues. The goal of our paper is to make a comparative study in realm of modern wireless trends such as Bluetooth, Wi-fi, Zigbee and Wibree and related facets.
The self configuring dexterous autonomy of MANET imposes some network challenges constrained to t... more The self configuring dexterous autonomy of MANET imposes some network challenges constrained to traditional dynamic routing behaviour. So as working with different mobility and traffic patterns with normal management schemes may lead some minor pitfalls to some important network performance parameters and hence can degrade the whole network performance. Here, Our aim is to make some DSR and MAC based cross layer optimizations and testify it on different mobility and traffic scenarios so as to justify the robustness of our proposed improvement.
WRKY proteins are a large family of plant-specific transcription factors associated with regulati... more WRKY proteins are a large family of plant-specific transcription factors associated with regulation of biotic and abiotic stress responses, but how they respond to cereal rust pathogens has never been explored at the molecular level. Full-length cDNA of TaWRKY1B was obtained from a wheat cultivar HD2329 derivative containing leaf rust resistance gene Lr28 based on domain characteristics. The unique feature of this WRKY transcription factor gene was the close proximity of the DNA-binding domain and consensus DNA element W-Box within the open reading frame. Infection with a virulent race of leaf rust fungus resulted in 146-fold induction of the gene in resistant plants, but only 12-fold in the susceptible plants as compared with mock-inoculated controls. Docking models of 74 amino acids DNA-binding domain and 26 bp W-Box element showed that the WRKY domain, located on the β1 strand, only interacts with the W-Box at positions corresponding to W125, R126, K127 and Y128 amino acids. A tr...
A Wireless Sensor Network(WSN) contains short range energy limited terminals/nodes in which multi... more A Wireless Sensor Network(WSN) contains short range energy limited terminals/nodes in which multiple nodes participates one by one to transfer data from source node to Base station. Each node appends some amount of delay which degrades the network performance as the jittered behaviour of network may not be allowed in some applications. Again the faulty nature of tandem nodes may create some severe routing scenarios. Concept of Broad Area Sensor Network (BASN) incorporates a real time communication between sensor node and Base station, which helps to optimize the Quality of Service (QoS). Our goal is to do extensive simulation on performance analysis of such sensor networks and its optimization.
ISPRS - International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences, 2014
Uttarakhand State of India suffered a widespread devastation in June 2013 due to floods caused by... more Uttarakhand State of India suffered a widespread devastation in June 2013 due to floods caused by excessive rain in the upper reaches of the Himalaya, glacial lake outburst flood (GLOF) and landslides. Restoration process in this mountainous State calls for scientifically sound planning so that the vulnerabilities and risks to such natural hazards are minimised and developmental processes are sustainable in long run. Towards this, an understanding of the patterns and major controls of damage of the recent disaster is a key requirement which can be achieved only if the primary data on locations and types of damage along with other local site conditions are available. Considering widespread damage, tough nature of terrain and the need for collecting the primary data on damage in shortest possible time, crowdsourcing approach was considered to be the most viable solution. Accordingly, a multiinstitutional initiative called "Map the Neighbourhood in Uttarakhand" (MANU) was con...
Defect depth profile study has been carried out in organic semiconductor (OSC) multilayers to cha... more Defect depth profile study has been carried out in organic semiconductor (OSC) multilayers to characterize the buried interfaces and layers using beam based positron annihilation spectroscopy. The bilayer and trilayer heterostructures (p-n, p-p and n-p-n) comprise of organic-organic and organic-inorganic (substrate) interfaces. Our study reveals the presence of defects at the interfaces whose concentration is seen to vary with the layer thickness. The S-W correlation has been used to examine the effect of organic materials as well as thickness of the layers on the defect microstructure in multilayers. The nature and type of defects in p-p bilayer are seen to be different as compared to p-n and n-p-n multilayers. Positron mobility in OSC layers has been calculated from the fitted diffusion length which is seen to be of the same order as the effective mobility of charge carrier obtained from the measured current density-voltage (J-V) characteristics. The role of structural defects and the intrinsic electric field at the interfaces on positron systematics is also examined. Positron diffusion modeling together with experimental data suggests that the defect at the interfaces has a stronger influence on the positron systematics than the intrinsic electric field across organic-organic interfaces.
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Papers by Ashwini Singh