Papers by Ashagrie gibtan
Ethiopia is a country in the horn of Africa endowed with several aquatic resources, including ove... more Ethiopia is a country in the horn of Africa endowed with several aquatic resources, including over 20 natural lakes, 12 large river basins, over 75 wetlands, and 15 reservoirs. Micro and macro-dam construction and river impoundment have intensified in recent years and created innumerable large and small water bodies. These water bodies can have great potential for capture and culture fisheries. Yet use of aquatic resources from these water resources is limited only small scale fish capture in some lakes and a few rivers. This book is the first attempt made to present aquaculture potential, cage culture study and national aquaculture development strategy of Ethiopia. It shows the general picture of the aquaculture situation in the country based on scarce data and professional experience. The book will provide a solid basis for further scientific studies for the scientists, aquaculturist, organization working in food security area and the public at large for the benefit of local commu...
North American Journal of Aquaculture
This article may be used for research, teaching, and private study purposes. Any substantial or s... more This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae, and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand, or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material.
This study was designed to investigate the antioxidative and angiotensin I-converting enzyme (ACE... more This study was designed to investigate the antioxidative and angiotensin I-converting enzyme (ACE) inhibitory activities of Eastern eel (Anguilla japonica). Hot water extracts were prepared over a period of 25 h at 100°C from the meat and whole body of the Eastern eel. Protein hydrolysates of Eastern eel were prepared over a period of 10 h using different hydrolytic enzymes, such as Alcalase, Protamex, and Alcalase + Protamex. The antioxidative and ACE inhibitory activities of the eel hydrolysates were higher than those of the extracts, and showed similar trends for hydrolysis time on each assay system due to similar enzyme specificity. The hydrolysates produced using Alcalase exhibited the highest DPPH scavenging activity (IC50=3.02 mg/mL), while the Protamex hydrolysates exhibited the highest ABTS (IC50=1.13 mg/mL), hydroxyl radical (IC50=1.30 mg/mL), and hydrogen peroxide (IC50=1.11 mg/mL) scavenging activities and ACE inhibitory activity (IC50=110.37 μg/mL). These results reveal...
International journal of systematic and evolutionary microbiology, Jan 11, 2017
A novel extremely halophilic archaeon, designated SAH-A6T, was isolated from a sample of commerci... more A novel extremely halophilic archaeon, designated SAH-A6T, was isolated from a sample of commercial rock salt in Ethiopia. Cells of SAH-A6T were aerobic and pleomorphic. The strain was able to grow at concentrations of 15-30 % (w/v) NaCl (optimum 20-25 % NaCl), at pH 6.0-9.0 (optimum pH 7.0) and in a temperature range of 30-55 °C (optimum 37-45 °C). Mg2+ was not required for growth of SAH-A6T cells. On the basis of 16S rRNA gene sequence analysis, strain SAH-A6T was closely related to Halorubrum halodurans Cb34T (99.1 %), Halorubrum rubrum YC87T (98.9 %), Halorubrum aquaticum EN-2T (98.7 %), Halorubrum cibi JCM 15757T (98.4 %), Halorubrum luteum CGSA15T (97.3 %), Halorubrum lipolyticum 9-3T (97.1 %), Halorubrum tibetense 8W8T (97.1 %), Halorubrum kocurii JCM 1478T (97.1 %), Halorubrum halophilum B8T (97.0 %) and Halorubrum persicum C49T (97.0 %). Phylogenetic analysis based on the rpoB' gene sequences showed that strain SAH-A6T was closely related to Hrr. halodurans Cb34T (99.7 ...
Frontiers in Microbiology
Salting is one of the oldest food preservation techniques. However, salt is also the source of li... more Salting is one of the oldest food preservation techniques. However, salt is also the source of living halophilic microorganisms that may affect human health. In order to determine the microbial communities of commercial salts, an investigation were done using amplicon sequencing approach in four commercial salts: Ethiopian Afdera salt (EAS), Ethiopian rock salt (ERS), Korean Jangpan salt (KJS), and Korean Topan salt (KTS). Using domain-specific primers, a region of the 16S rRNA gene was amplified and sequenced using a Roche 454 instrument. The results indicated that these microbial communities contained 48.22-61.4% Bacteria, 37.72-51.26% Archaea, 0.51-0.86% Eukarya, and 0.005-0.009% unclassified reads. Among bacteria, the communities in these salts were dominated by the phyla Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes. Of the archaea, 91.58% belonged to the class Halobacteria, whereas the remaining 7.58, 0.83, and 0.01% were Nanoarchaea, Methanobacteria, and Thermococci, respectively. This comparison of microbial diversity in salts from two countries showed the presence of many archaeal and bacterial genera that occurred in salt samples from one country but not the other. The bacterial genera Enterobacter and Halovibrio were found only in Korean and Ethiopian salts, respectively. This study indicated the occurrence and diversity of halophilic bacteria and archaea in commercial salts that could be important in the gastrointestinal tract after ingestion.
Genome Announcements, 2015
Here we report the draft whole-genome sequence (3.72 Mbp) of Bacillus sp. strain SB49, an extreme... more Here we report the draft whole-genome sequence (3.72 Mbp) of Bacillus sp. strain SB49, an extremely halophilic bacterium isolated from a salt crystallizer pond of the Little Rann of Kutch in India. Unraveling the genome of this organism will facilitate understanding and isolation of the genes involved in imparting extreme osmotolerance.
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Papers by Ashagrie gibtan