AimsWe set out to characterize the voiding phenotypes of male mice to a water avoidance stress (W... more AimsWe set out to characterize the voiding phenotypes of male mice to a water avoidance stress (WAS) protocol and compare the molecular changes with those induced by surgically induced partial bladder outlet obstruction (pBOO).MethodsSix‐week‐old male Swiss Webster mice housed with sibling littermates were individually placed on a platform centered in the middle of a water filled basin for 1 hr daily for 4 weeks. A non stressed cohort of sibling littermates served as controls. Measured end points included voiding frequency, voided volume, bladder mass, and in vivo cystometry. Molecular end points included myosin heavy chain (MHC) isoform distribution by PCR, and nuclear translocation of hypoxia inducible factor (HIF1α) and the nuclear factor of activated T‐cells (NFAT) by gel shift assay. These molecular endpoints were compared with samples from male mice undergoing anatomic pBOO.ResultsWAS resulted in increased average voided volumes and bladder mass, and a decrease in voiding freq...
was compared with that on day 13-16 and that of adults by relative power spectral density (rPSD) ... more was compared with that on day 13-16 and that of adults by relative power spectral density (rPSD) calculated with Fast Fourier Transform. RESULTS: AVSOP method could retrieve micturition data from infant mice, with a distinct circadian rhythmicity (one of infant male data shown in figure). VV and UV increased considerably along with age until 6 weeks old. Freq and micturition rhythmicities in male mice were similar between post-weaning pups, adolescents around 5-6 weeks, and adults at 8 weeks, while female mice developed these factors along with age. For example, values of rPSD of Freq in male were 0.10Ϯ0.02, 0.15Ϯ0.03 and 0.13Ϯ0.04, respectively and in female were 0.07Ϯ0.01, 0.13Ϯ0.01 and 0.21Ϯ0.03, respectively (meanϮs.d.). CONCLUSIONS: Development of micturition after weaning was largely characterized by increases of VV and UV. The Freq and micturition rhythmicities seem to be established by weaning in male but not yet in female. These data could be basic informations for establishing mouse model of developmental micturition disorder, such as nocturnal enuresis.
Purpose/objective Long-term complications from anti-incontinence surgical procedures are rarely r... more Purpose/objective Long-term complications from anti-incontinence surgical procedures are rarely reported. We report on delayed presentation of complications relating to the synthetic bolster placed for the Stamey bladder neck suspension. Materials and methods: Patients undergoing re-operative surgery following prior Stamey endoscopic bladder neck suspension were selected from a surgical database. Four women with lower urinary tract and/or vaginal symptoms following prior Stamey endoscopic bladder neck suspension were identified. All patients had undergone removal of the bolster material by a single surgeon (ESR) at re-operation. Preoperative, operative, and postoperative inpatient and outpatient records were reviewed. Results: Patients presented with a variety of symptoms including urinary incontinence, recurrent cystitis, vaginitis, and urinary frequency at 9, 11, 11, and 12 years after Stamey bladder neck suspension. In addition, two patients presented with recurrent, intermittent bloody vaginal discharge and two patients complained of recurrent urinary tract infections and irritative voiding symptoms. All patients underwent transvaginal excision of the Dacron bolster. Three patients also underwent placement of an autologous pubovaginal sling for symptomatic recurrent stress urinary incontinence. At a mean follow-up of 30 months all four patients were improved. There was no recurrence of vaginal discharge or urinary tract infections. Irritative voiding symptoms resolved. Conclusions: Delayed complications from surgically implanted synthetic materials can present many years after initial implanta-tion. The clinical findings are often subtle and require a high degree of suspicion. Vaginal discharge and irritative urinary symptoms in patients with even a remote history of Stamey bladder neck suspension should prompt a thorough vaginal exam and cystoscopy. Excision of the bolsters can be performed and is usually followed by symptomatic improvement.
AimsWe set out to characterize the voiding phenotypes of male mice to a water avoidance stress (W... more AimsWe set out to characterize the voiding phenotypes of male mice to a water avoidance stress (WAS) protocol and compare the molecular changes with those induced by surgically induced partial bladder outlet obstruction (pBOO).MethodsSix‐week‐old male Swiss Webster mice housed with sibling littermates were individually placed on a platform centered in the middle of a water filled basin for 1 hr daily for 4 weeks. A non stressed cohort of sibling littermates served as controls. Measured end points included voiding frequency, voided volume, bladder mass, and in vivo cystometry. Molecular end points included myosin heavy chain (MHC) isoform distribution by PCR, and nuclear translocation of hypoxia inducible factor (HIF1α) and the nuclear factor of activated T‐cells (NFAT) by gel shift assay. These molecular endpoints were compared with samples from male mice undergoing anatomic pBOO.ResultsWAS resulted in increased average voided volumes and bladder mass, and a decrease in voiding freq...
was compared with that on day 13-16 and that of adults by relative power spectral density (rPSD) ... more was compared with that on day 13-16 and that of adults by relative power spectral density (rPSD) calculated with Fast Fourier Transform. RESULTS: AVSOP method could retrieve micturition data from infant mice, with a distinct circadian rhythmicity (one of infant male data shown in figure). VV and UV increased considerably along with age until 6 weeks old. Freq and micturition rhythmicities in male mice were similar between post-weaning pups, adolescents around 5-6 weeks, and adults at 8 weeks, while female mice developed these factors along with age. For example, values of rPSD of Freq in male were 0.10Ϯ0.02, 0.15Ϯ0.03 and 0.13Ϯ0.04, respectively and in female were 0.07Ϯ0.01, 0.13Ϯ0.01 and 0.21Ϯ0.03, respectively (meanϮs.d.). CONCLUSIONS: Development of micturition after weaning was largely characterized by increases of VV and UV. The Freq and micturition rhythmicities seem to be established by weaning in male but not yet in female. These data could be basic informations for establishing mouse model of developmental micturition disorder, such as nocturnal enuresis.
Purpose/objective Long-term complications from anti-incontinence surgical procedures are rarely r... more Purpose/objective Long-term complications from anti-incontinence surgical procedures are rarely reported. We report on delayed presentation of complications relating to the synthetic bolster placed for the Stamey bladder neck suspension. Materials and methods: Patients undergoing re-operative surgery following prior Stamey endoscopic bladder neck suspension were selected from a surgical database. Four women with lower urinary tract and/or vaginal symptoms following prior Stamey endoscopic bladder neck suspension were identified. All patients had undergone removal of the bolster material by a single surgeon (ESR) at re-operation. Preoperative, operative, and postoperative inpatient and outpatient records were reviewed. Results: Patients presented with a variety of symptoms including urinary incontinence, recurrent cystitis, vaginitis, and urinary frequency at 9, 11, 11, and 12 years after Stamey bladder neck suspension. In addition, two patients presented with recurrent, intermittent bloody vaginal discharge and two patients complained of recurrent urinary tract infections and irritative voiding symptoms. All patients underwent transvaginal excision of the Dacron bolster. Three patients also underwent placement of an autologous pubovaginal sling for symptomatic recurrent stress urinary incontinence. At a mean follow-up of 30 months all four patients were improved. There was no recurrence of vaginal discharge or urinary tract infections. Irritative voiding symptoms resolved. Conclusions: Delayed complications from surgically implanted synthetic materials can present many years after initial implanta-tion. The clinical findings are often subtle and require a high degree of suspicion. Vaginal discharge and irritative urinary symptoms in patients with even a remote history of Stamey bladder neck suspension should prompt a thorough vaginal exam and cystoscopy. Excision of the bolsters can be performed and is usually followed by symptomatic improvement.
Uploads
Papers by Ariana Smith