Papers by Ardeshir Bahmanimehr
Laboratory Medicine
Objective The main concern about measuring the concentration of rivaroxaban by anti-Xa assay in s... more Objective The main concern about measuring the concentration of rivaroxaban by anti-Xa assay in some laboratories is the lack of a commercial specific calibrator in emergencies. Therefore, this study aimed at providing a homemade anti-Xa calibrator and commercial low molecular weight heparin (LMWH) anti-Xa calibrator. Methods The anti-Xa plasma concentration of rivaroxaban was measured in 70 patients using a commercial specific anti-Xa calibrator, a commercial LMWH anti-Xa calibrator, and a homemade anti-Xa calibrator. Results We demonstrated a significant correlation and agreement (P < .001) between LMWH-calibrated anti-Xa and the commercial specific calibrator. A significant correlation (P < .001) was found between homemade calibrated anti-Xa made by normal pooled plasma and that calibrated with a commercial specific drug. The nonspecific homemade and LMWH calibrators had excellent agreement (P < .001) and can be used interchangeably. Conclusion Our data showed that for e...
Iranian Journal of Basic Medical Sciences, 2021
Objective(s): Many patients die due to vascular, gastrointestinal lumen problems, and coronary he... more Objective(s): Many patients die due to vascular, gastrointestinal lumen problems, and coronary heart diseases. Synthetic vessels that are made of biodegradable-nanofiber polymers have significant properties such as proper biodegradability and efficient physical properties such as high strength and flexibility. Some of the best options for supporting cells in soft tissue engineering and design are applications of thermoplastic polyurethane polymer in the venous tissue. In this study, the first nanoparticle-reinforced polymeric artificial prosthesis was designed and tested to be used in the human body. Materials and Methods: In this study, artificial gastrointestinal lumen were fabricated and prepared using a 3D printer. To improve cell adhesion, wettability properties and mechanical stability of elastin biopolymer with magnetic nanoparticles (MNPs) as well as single-walled carbon nanotubes (SWCNT) were prepared as separate filaments. MNPs were made in 5–7 mm sizes and then examined f...
Iranian Journal of Basic Medical Sciences, 2014
Objective(s): To determine the fetal discernment in suspected cases of sex linked recessive disea... more Objective(s): To determine the fetal discernment in suspected cases of sex linked recessive disease in the first trimester of pregnancy. Materials and Methods: After collection of 100 Chorionic Villi samples, the DNAs were extracted and baby gender was determined. Meanwhile, after increasing the sensitivity, the system was able to detect the sex of each cell which was obtained by biopsy. Results: Early fetal gender of 100 Chorionic Villi samples were assessed by PCR. After increasing sensitivity of the assay, the sexes in 13 fetuses that were in different cellular stages were detected. Morover, sexes were detected in two unfertilized and one fertilized ovum but without any division. Conclusion: Sex detection of fetus before delivery in the first trimester of pregnancy, will prevent babies with abnormalities being born. It can also be used in detection of recessive sex related diseases in In Vitro Fertilization cases for sex detection and to transfer female fetus to the mother. Our o...
Metabotropic glutamate receptor 5 (mGluR5) regulates excitatory postsynaptic signaling in the cen... more Metabotropic glutamate receptor 5 (mGluR5) regulates excitatory postsynaptic signaling in the central nervous system (CNS) and is implicated in various CNS disorders. Protein kinase A (PKA) signaling is known to play a critical role in neuropsychiatric disorders such as Parkinson's disease, schizophrenia and addiction. Dopamine signaling is known to modulate the properties of mGluR5 in a cAMP-and PKA-dependent manner, suggesting that mGluR5 may be a direct target for PKA. Our study identifies mGluR5 at Ser870 as a direct substrate for PKA phosphorylation and demonstrates that this phosphorylation plays a critical role in the PKA-mediated modulation of mGluR5 functions such as extracellular signal-regulated kinase (ERK) phosphorylation and intracellular Ca 2+ oscillations. The identification of the molecular mechanism by which PKA signaling modulates mGluR5-mediated cellular responses contributes to the understanding of the interaction between dopaminergic and glutamatergic neuronal signaling.
iranian journal of epidemiology, 2014
See next page for additional authors This Open Access Preprint is brought to you for free and ope... more See next page for additional authors This Open Access Preprint is brought to you for free and open access by the WSU Press at DigitalCommons@WayneState. It has been accepted for inclusion in Human Biology Open Access Pre-Prints by an authorized administrator of DigitalCommons@WayneState. Recommended Citation
Journal of Cell and Molecular Research, 2016
The first step in the prenatal diagnosis of X-linked genetic disorders is determining fetus gende... more The first step in the prenatal diagnosis of X-linked genetic disorders is determining fetus gender. Current invasive methods to obtain the DNA source of the fetus instead of its miscarriage risk, has harmful stress for high risk pregnancies. Cell free fetal DNA (cffDNA) circulating in the maternal blood, has now become a useful source of noninvasive prenatal diagnosis. Considering limitation of cffDNA; as its small fragment size and low concentration in maternal plasma; using this source for clinical diagnostic material, requires a high efficiency extraction method and reasonable molecular tests to lead more accurate results.In the current study, we optimized Triton/Heat/Phenol (THP) protocol for extracting cffDNA in 8 and 12 weeks gestation. Fetal sex determined for prenatal diagnosis of hemophilia using SRY gene markers and high resolution markers of sex chromosomes by QF-PCR. The results compared with genetic tests on CVS samples. We confirmed the persistence of fetal DNA in mate...
Meta Gene, 2021
Cytochrome P450 3A4 (CYP3A4), the main drug-metabolizing enzyme in the liver, has a functional SN... more Cytochrome P450 3A4 (CYP3A4), the main drug-metabolizing enzyme in the liver, has a functional SNP in intron six named CYP3A4⁎22. Given the CYP3A4⁎22 polymorphism effect on the reduced CYP3A4 mRNA/protein expression, this study aimed to evaluate the prevalence of CYP3A4⁎22 polymorphism in Iranian patients with stroke compared to the control group. A total of 175 patients with stroke and 236 control age-sex matched ones have been examined for CYP3A4⁎22 polymorphism by Tetra ARMS-PCR methods. The results were confirmed by the PCR-RFLP technique and the sequencing of DNA. This study is the first report of CYP3A4⁎22 polymorphism from Iran. The frequency of the CYP3A4⁎22 wild-type (GG genotype) and heterozygous genotypes (GA genotype) was 93.1% and 6.9% in patients, and 92.8% and 7.2% in the controls, respectively. None of the participants was the recessive model for this polymorphism. There were no statistical differences between the patient and control groups (p > 0.05). Also, the G...
Archives of Iranian medicine, 2018
BACKGROUND Blood gas analysis is very important in the patients with respiratory problems. Arteri... more BACKGROUND Blood gas analysis is very important in the patients with respiratory problems. Arterial puncture may cause complications such as pain, local hematoma, infection and nerve injury. The procedure itself can be technically difficult. In contrast, venous sampling is an easier procedure with fewer complications. Therefore, this study aims to determine the possibility of replacement of venous blood gas (VBG) values by ABG values in ICU wards admitted patients. METHODS In this study, 155 paired blood gas samples collected from patients admitted to ICU wards in Motahari hospital, Marvdasht, Fars, Iran. Statistical means of blood gas parameters, including PO2, PCO2, HCO3 and PH have been compared in both, arterial and venous, blood samples in parallel using paired t-test. RESULTS Mean difference of arterial and venous gas parameters, PO2, PCO2 and HCO3 , was significantly differ. All paired gas parameters in arterial and venous blood samples were significantly correlated, while th...
B ase d on contempor ary DNA distributions and cautious inferences o n migratory histories, consi... more B ase d on contempor ary DNA distributions and cautious inferences o n migratory histories, considering previous ly localized the potential geographic origin of haplogroup G - M201, by using high - resolution phylogenetic relationships containing both G1 - M285 - and G2 - P287 - related lineages , this research tries to find some genetic evidence s to trace the spread of early Neolithic agriculture in the Northwest of Iran. For Iranian Azeris, the presence of haplogroup G - M201 has been associated with the spread of agriculture, as it report ed especially for the European context (King RJ, et al., 2011). Considering general frequency pattern of haplogroup G in Iranians, it could be inferred that m ountainous and unique harsh geography of west and northwest of Iran might facilitated the establis hment of specific lineage of Neolithic agriculturalists , e.g. G haplogroup, in the region. However uninhabited Great Salt Desert created particular sharp border in the center and east of th...
Annals of Hematology, 2021
Over the past 20 years, hydroxyurea (HU) has emerged as an effective therapeutic agent in thalass... more Over the past 20 years, hydroxyurea (HU) has emerged as an effective therapeutic agent in thalassemic patients to improve anemia and decrease the transfusion dependency. We evaluated long-term safety and clinical response to HU in patients with non-transfusion-dependent β-thalassemia (NTDT). In this retrospective study, medical records of 181 patients with NTDT were evaluated during October to December 2020 in Southern Iran. No requirement to blood transfusion was considered as sustained transfusion independence response. All patients were regularly examined and monitored for the occurrence of any adverse event (AE) of HU. The mean duration of HU consumption ± SD was 18.2 ± 4.0 (8–22) years. Overall, 149 patients (82.3%) had sustained transfusion independence response. β-globin gene mutations and XmnI polymorphisms were not significantly associated with clinical response (P > 0.05). Mild and transient AEs were reported in 60 patients (33%) with no requirement to drug interruption. Hydroxyurea with the dose of 8–15 mg/kg can be used as a safe and effective treatment in NTDT patients. It was well tolerated in long term without any serious complication or secondary malignancy. No relationship between XmnI or β-globin gene mutations with HU response was observed in this geographic area of the world.
Women’s Health Bulletin, 2018
Background: Assisted reproductive technology (ART) helps infertility treatment. In this study, th... more Background: Assisted reproductive technology (ART) helps infertility treatment. In this study, the researchers tried to find several prognostic factors for ART success. Methods: Data of 189 couples that had undergone ART at Qadir-Mother and Child hospital, were retrospectively explored. Cycles were classified as succeeded/failed and female/male/combined causes. Three classes of ovarian response based on the number of retrieved oocytes were determined. Duration of ovarian stimulation, dosages of gonadotropins, and sperm parameters were evaluated. Chi square, t-test and Pearson correlation were used for the analysis. Results: Couples with male factor infertility had a higher success rate. Female age had a negative correlation with follicle count (r =-0.25), retrieved oocytes (r =-0.25), fertilized oocytes (r=-0.2), and clinical pregnancy (r =-0.21). Female age was negatively correlated with AMH, although not significant. The fertilization rate had a reverse correlation with the basal FSH (r =-0.22, P= 0.01) and LH (r=-0.18, P= 0.05). Finally, ART success rate was positively correlated with the number of follicles before (r = 0.18, P = 0.05) and after retrieval (r = 0.15, P = 0.05) and fertilized oocyte (r = 0.23, P = 0.01). Conclusions: Female age, the number of mature follicles before retrieval, the number of retrieved oocyte, and fertilized oocytes are predictive factors in ART outcomes.
International journal of fertility & sterility, 2018
Y chromosome deletions (YCDs) in azoospermia factor (AZF) region are associated with abnormal spe... more Y chromosome deletions (YCDs) in azoospermia factor (AZF) region are associated with abnormal spermatogenesis and may lead to azoospermia or severe oligozoospermia. Assisted reproductive technologies (ART) by intracytoplasmic sperm injection (ICSI) and testicular sperm extraction (TESE) are commonly required for infertility management of patients carrying YCDs. The aim of this study was to estimate the frequency of YCDs, to find the most frequent variant in infertile men candidate for ART and to compare YCD distribution with a control fertile group. The semen parameters, hormonal profiles and ART outcomes of the infertile group were studied. This case-control study consisted of 97 oligozoospermic or non-obstructive azoospermic (NOA) infertile men, who had undergone ART, as the case group and 100 fertile men as the control group. DNA samples were extracted from blood samples taken from all 197 participants and YCDs were identified by multiplex polymerase chain reaction (PCR) of eight...
Scientific reports, Apr 7, 2017
Human mitochondrial DNA haplogroup U is among the initial maternal founders in Southwest Asia and... more Human mitochondrial DNA haplogroup U is among the initial maternal founders in Southwest Asia and Europe and one that best indicates matrilineal genetic continuity between late Pleistocene hunter-gatherer groups and present-day populations of Europe. While most haplogroup U subclades are older than 30 thousand years, the comparatively recent coalescence time of the extant variation of haplogroup U7 (~16-19 thousand years ago) suggests that its current distribution is the consequence of more recent dispersal events, despite its wide geographical range across Europe, the Near East and South Asia. Here we report 267 new U7 mitogenomes that - analysed alongside 100 published ones - enable us to discern at least two distinct temporal phases of dispersal, both of which most likely emanated from the Near East. The earlier one began prior to the Holocene (~11.5 thousand years ago) towards South Asia, while the later dispersal took place more recently towards Mediterranean Europe during the ...
Human biology, 2013
The origin and history of the Ashkenazi Jewish population have long been of great interest, and a... more The origin and history of the Ashkenazi Jewish population have long been of great interest, and advances in high-throughput genetic analysis have recently provided a new approach for investigating these topics. We and others have argued on the basis of genome-wide data that the Ashkenazi Jewish population derives its ancestry from a combination of sources tracing to both Europe and the Middle East. It has been claimed, however, through a reanalysis of some of our data, that a large part of the ancestry of the Ashkenazi population originates with the Khazars, a Turkic-speaking group that lived to the north of the Caucasus region ~1,000 years ago. Because the Khazar population has left no obvious modern descendants that could enable a clear test for a contribution to Ashkenazi Jewish ancestry, the Khazar hypothesis has been difficult to examine using genetics. Furthermore, because only limited genetic data have been available from the Caucasus region, and because these data have been ...
The Turkic peoples represent a diverse collection of ethnic groups defined by the Turkic language... more The Turkic peoples represent a diverse collection of ethnic groups defined by the Turkic languages. These groups have dispersed across a vast area, including Siberia, Northwest China, Central Asia, East Europe, the Caucasus, Anatolia, the Middle East, and Afghanistan. The origin and early dispersal history of the Turkic peoples is disputed, with candidates for their ancient homeland ranging from the Transcaspian steppe to Manchuria in Northeast Asia. Previous genetic studies have not identified a clear-cut unifying genetic signal for the Turkic peoples, which lends support for language replacement rather than demic diffusion as the model for the Turkic language's expansion. We addressed the genetic origin of 373 individuals from 22 Turkic-speaking populations, representing their current geographic range, by analyzing genome-wide high-density genotype data. Most of the Turkic peoples studied, except those in Central Asia, genetically resembled their geographic neighbors, in agree...
PLoS ONE, 2013
Ethnic Belarusians make up more than 80% of the nine and half million people inhabiting the Repub... more Ethnic Belarusians make up more than 80% of the nine and half million people inhabiting the Republic of Belarus. Belarusians together with Ukrainians and Russians represent the East Slavic linguistic group, largest both in numbers and territory, inhabiting East Europe alongside Baltic-, Finno-Permic-and Turkic-speaking people. Till date, only a limited number of low resolution genetic studies have been performed on this population. Therefore, with the phylogeographic analysis of 565 Y-chromosomes and 267 mitochondrial DNAs from six well covered geographic sub-regions of Belarus we strove to complement the existing genetic profile of eastern Europeans. Our results reveal that around 80% of the paternal Belarusian gene pool is composed of R1a, I2a and N1c Y-chromosome haplogroups-a profile which is very similar to the two other eastern European populations-Ukrainians and Russians. The maternal Belarusian gene pool encompasses a full range of West Eurasian haplogroups and agrees well with the genetic structure of central-east European populations. Our data attest that latitudinal gradients characterize the variation of the uniparentally transmitted gene pools of modern Belarusians. In particular, the Y-chromosome reflects movements of people in central-east Europe, starting probably as early as the beginning of the Holocene. Furthermore, the matrilineal legacy of Belarusians retains two rare mitochondrial DNA haplogroups, N1a3 and N3, whose phylogeographies were explored in detail after de novo sequencing of 20 and 13 complete mitogenomes, respectively, from all over Eurasia. Our phylogeographic analyses reveal that two mitochondrial DNA lineages, N3 and N1a3, both of Middle Eastern origin, might mark distinct events of matrilineal gene flow to Europe: during the mid-Holocene period and around the Pleistocene-Holocene transition, respectively.
PLoS ONE, 2013
Due to its pivotal geographical location and proximity to transcontinental migratory routes, Iran... more Due to its pivotal geographical location and proximity to transcontinental migratory routes, Iran has played a key role in subsequent migrations, both prehistoric and historic, between Africa, Asia and Europe. To shed light on the genetic structure of the Iranian population as well as on the expansion patterns and population movements which affected this region, the complete mitochondrial genomes of 352 Iranians were obtained. All Iranian populations studied here exhibit similarly high diversity values comparable to the other groups from the Caucasus, Anatolia and Europe. The results of AMOVA and MDS analyses did not associate any regional and/or linguistic group of populations in the Anatolia/Caucasus and Iran region pointing to close genetic positions of Persians and Qashqais to each other and to Armenians, and Azeris from Iran to Georgians. By reconstructing the complete mtDNA phylogeny of haplogroups R2,
European Journal of Human Genetics, 2012
Haplogroup G, together with J2 clades, has been associated with the spread of agriculture, especi... more Haplogroup G, together with J2 clades, has been associated with the spread of agriculture, especially in the European context. However, interpretations based on simple haplogroup frequency clines do not recognize underlying patterns of genetic diversification. Although progress has been recently made in resolving the haplogroup G phylogeny, a comprehensive survey of the geographic distribution patterns of the significant sub-clades of this haplogroup has not been conducted yet. Here we present the haplogroup frequency distribution and STR variation of 16 informative G sub-clades by evaluating 1472 haplogroup G chromosomes belonging to 98 populations ranging from Europe to Pakistan. Although no basal G-M201* chromosomes were detected in our data set, the homeland of this haplogroup has been estimated to be somewhere nearby eastern Anatolia, Armenia or western Iran, the only areas characterized by the co-presence of deep basal branches as well as the occurrence of high sub-haplogroup diversity. The P303 SNP defines the most frequent and widespread G sub-haplogroup. However, its sub-clades have more localized distribution with the U1-defined branch largely restricted to Near/Middle Eastern and the Caucasus, whereas L497 lineages essentially occur in Europe where they likely originated. In contrast, the only U1 representative in Europe is the G-M527 lineage whose distribution pattern is consistent with regions of Greek colonization. No clinal patterns were detected suggesting that the distributions are rather indicative of isolation by distance and demographic complexities.
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Papers by Ardeshir Bahmanimehr