Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 1993
Comparative sequence analyses were performed on 14 genes encoding bacterial elongation factors EF... more Comparative sequence analyses were performed on 14 genes encoding bacterial elongation factors EF-Tu and 7 genes encoding the β-subunit of bacterial F1F0 type ATP-synthases. The corresponding predicted amino acid sequences were compared with published primary structures of homologous molecules. Phylogenetic trees were reconstructed from both data sets of aligned protein sequences and from an equivalent selection of 16S rRNA sequences by applying distance matrix and maximum parsimony methods. The EF-Tu data were in very good agreement with the rRNA data, although the resolution within the EF-Tu tree was reduced at certain phylogenetic levels. The resolution power of the ATPase β-subunit sequence data were more reduced than those of the EF-Tu data. In comparison with the 16S rRNA tree there are minor differences in the order of adjacent branchings within the ATPase β-subunit tree.
Mononuclear iron(III) nitrilotriacetato dichloride, [Fe(nta)Cl 2 ] 2( , and iron(III) bis-iminodi... more Mononuclear iron(III) nitrilotriacetato dichloride, [Fe(nta)Cl 2 ] 2( , and iron(III) bis-iminodiacetato, [Fe(ida) 2 ] ( , complexes were formed in py, from which they were isolated and characterized by X-ray crystallography. The iron Á/nta complex is the structurally simplest of the reported solid state iron Á/nta complexes, and is catalytic for the air oxidation of hydrogen sulfide to sulfur. # (M.A. Walters).
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 1988
A number of Escherichia coli strains have been isolated from dogs with urinary tract infections. ... more A number of Escherichia coli strains have been isolated from dogs with urinary tract infections. These strains have been characterised with respect to their O, K, H, and fimbrial antigens, colicin production, antibiotic resistance, plasmid content and their ability to haemagglutinate erythrocytes from various species. Crossed immunoelectrophoresis of fimbrial extracts, as well as the reaction of partly purified fimbriae of a number of these strains with monoclonal antibodies revealed homology or a strong crossereaction with an F12 fimbrial subunit protein of human uropathogenic E. coli strains. Unlike human F12 fimbriae producing strains, the dog isolates did agglutinate dog erythrocytes in the presence of D-mannose but not human erythrocytes, indicating that the adhesin carried by these strains is different from the adhesin on fimbriae of human uropathogenic E. coli. Similar indications were obtained from experiments with latex beads coated with the receptor for P-fimbriae. These beads were agglutinated by Escherichia coli strains from human urinary tract infections, but not by the dog isolates described here. Preliminary adhesion experiments of human and dog Escherichia coli to human bladder epithelial and canine kidney epithelial cells also showed differences in adhesion depending on the origin of the strain tested.
Insulin secretion from pancreatic -cells is mediated by the opening of voltage-gated Ca 2؉ chann... more Insulin secretion from pancreatic -cells is mediated by the opening of voltage-gated Ca 2؉ channels (Ca V ) and exocytosis of insulin dense core vesicles facilitated by the secretory soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein machinery. We previously observed that -cell exocytosis is sensitive to the acute removal of membrane cholesterol. However, less is known about the chronic changes in endogenous cholesterol and its biosynthesis in regulating -cell stimulus-secretion coupling. We examined the effects of inhibiting endogenous -cell cholesterol biosynthesis by using the squalene epoxidase inhibitor, NB598. The expression of squalene epoxidase in primary and clonal -cells was confirmed by RT-PCR. Cholesterol reduction of 36 -52% was observed in MIN6 cells, mouse and human pancreatic islets after a 48-h incubation with 10 M NB598. A similar reduction in cholesterol was observed in the subcel-
Abstract: Human skeletal muscle stem cells from healthy donors aged 2–82 years (n = 13) and from... more Abstract: Human skeletal muscle stem cells from healthy donors aged 2–82 years (n = 13) and from three children suffering from Duchenne Muscular Dystrophy (DMD) were implanted into soleus muscles of immunoincompetent mice and were also expanded in vitro until senescence. Growth of implanted cells was quantified by structural features and by the amount of human DNA present in a muscle. Proliferative capacity in vitro and in vivo was inversely related to age of the donor. In vitro, a decline of about two mean population doublings (MPDs) per 10 years of donor’s age was observed. Muscle stem cells from DMD children were prematurely aged. In general, cell preparations with low or decreasing content in desmin-positive cells produced more MPDs than age-matched high-desmin preparations and upon implantation more human DNA and more nonmyogenic than myogenic tissue. Thus, a “Desmin Factor” was derived which predicts “quality” of the human muscle tissue growing in vivo. This factor may serve as a prognostic tool.
Oslo University College, Faculty of Nursing places undergraduate students in peer learning partne... more Oslo University College, Faculty of Nursing places undergraduate students in peer learning partnerships: 3rd year students as tutors, and 1st year students as tutees during practical skill learning. A study was designed to investigate this peer learning project. One of the research questions in particular on the role of the tutors: How do 3rd year nursing students carry out their role as supervisors for 1st year students in the skills laboratory? The study had an exploratory design. Qualitative data was gathered by three members of a research team, using video recordings of the supervised sessions and focus group interviews with both 1st and 3rd year students. Data collection was repeated in 2006e2007e2008 in different groups of students. Four identified themes illustrate how the 3rd year students supervise during the performance of the procedures in the skills laboratory:
Background: The postnatal development of the epididymis is a complex process that results in a hi... more Background: The postnatal development of the epididymis is a complex process that results in a highly differentiated epithelium, divided into several segments. Recent studies indicate a role for RNA interference (RNAi) in the development of the epididymis, however, the actual requirement for RNAi has remained elusive. Here, we present the first evidence of a direct need for RNAi in the differentiation of the epididymal epithelium.
This paper reviews EU regulation on international roaming and discusses whether this will lead to... more This paper reviews EU regulation on international roaming and discusses whether this will lead to more cost orientation when setting roaming charges in Europe. First, a cost analysis for providing international roaming is presented. Then, the various proposals put forward during the debate in the EU Parliament are discussed. Finally, the issue of cost orientation is discussed.
The purification of a 6x-histidine tagged viral coat protein (L1) in expanded mode directly follo... more The purification of a 6x-histidine tagged viral coat protein (L1) in expanded mode directly following chemical extraction from the cytoplasm of Escherichia coli HMS174(DE3) is investigated. Chelating adsorbents based on the ligands 21 21
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 1993
Comparative sequence analyses were performed on 14 genes encoding bacterial elongation factors EF... more Comparative sequence analyses were performed on 14 genes encoding bacterial elongation factors EF-Tu and 7 genes encoding the β-subunit of bacterial F1F0 type ATP-synthases. The corresponding predicted amino acid sequences were compared with published primary structures of homologous molecules. Phylogenetic trees were reconstructed from both data sets of aligned protein sequences and from an equivalent selection of 16S rRNA sequences by applying distance matrix and maximum parsimony methods. The EF-Tu data were in very good agreement with the rRNA data, although the resolution within the EF-Tu tree was reduced at certain phylogenetic levels. The resolution power of the ATPase β-subunit sequence data were more reduced than those of the EF-Tu data. In comparison with the 16S rRNA tree there are minor differences in the order of adjacent branchings within the ATPase β-subunit tree.
Mononuclear iron(III) nitrilotriacetato dichloride, [Fe(nta)Cl 2 ] 2( , and iron(III) bis-iminodi... more Mononuclear iron(III) nitrilotriacetato dichloride, [Fe(nta)Cl 2 ] 2( , and iron(III) bis-iminodiacetato, [Fe(ida) 2 ] ( , complexes were formed in py, from which they were isolated and characterized by X-ray crystallography. The iron Á/nta complex is the structurally simplest of the reported solid state iron Á/nta complexes, and is catalytic for the air oxidation of hydrogen sulfide to sulfur. # (M.A. Walters).
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 1988
A number of Escherichia coli strains have been isolated from dogs with urinary tract infections. ... more A number of Escherichia coli strains have been isolated from dogs with urinary tract infections. These strains have been characterised with respect to their O, K, H, and fimbrial antigens, colicin production, antibiotic resistance, plasmid content and their ability to haemagglutinate erythrocytes from various species. Crossed immunoelectrophoresis of fimbrial extracts, as well as the reaction of partly purified fimbriae of a number of these strains with monoclonal antibodies revealed homology or a strong crossereaction with an F12 fimbrial subunit protein of human uropathogenic E. coli strains. Unlike human F12 fimbriae producing strains, the dog isolates did agglutinate dog erythrocytes in the presence of D-mannose but not human erythrocytes, indicating that the adhesin carried by these strains is different from the adhesin on fimbriae of human uropathogenic E. coli. Similar indications were obtained from experiments with latex beads coated with the receptor for P-fimbriae. These beads were agglutinated by Escherichia coli strains from human urinary tract infections, but not by the dog isolates described here. Preliminary adhesion experiments of human and dog Escherichia coli to human bladder epithelial and canine kidney epithelial cells also showed differences in adhesion depending on the origin of the strain tested.
Insulin secretion from pancreatic -cells is mediated by the opening of voltage-gated Ca 2؉ chann... more Insulin secretion from pancreatic -cells is mediated by the opening of voltage-gated Ca 2؉ channels (Ca V ) and exocytosis of insulin dense core vesicles facilitated by the secretory soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein machinery. We previously observed that -cell exocytosis is sensitive to the acute removal of membrane cholesterol. However, less is known about the chronic changes in endogenous cholesterol and its biosynthesis in regulating -cell stimulus-secretion coupling. We examined the effects of inhibiting endogenous -cell cholesterol biosynthesis by using the squalene epoxidase inhibitor, NB598. The expression of squalene epoxidase in primary and clonal -cells was confirmed by RT-PCR. Cholesterol reduction of 36 -52% was observed in MIN6 cells, mouse and human pancreatic islets after a 48-h incubation with 10 M NB598. A similar reduction in cholesterol was observed in the subcel-
Abstract: Human skeletal muscle stem cells from healthy donors aged 2–82 years (n = 13) and from... more Abstract: Human skeletal muscle stem cells from healthy donors aged 2–82 years (n = 13) and from three children suffering from Duchenne Muscular Dystrophy (DMD) were implanted into soleus muscles of immunoincompetent mice and were also expanded in vitro until senescence. Growth of implanted cells was quantified by structural features and by the amount of human DNA present in a muscle. Proliferative capacity in vitro and in vivo was inversely related to age of the donor. In vitro, a decline of about two mean population doublings (MPDs) per 10 years of donor’s age was observed. Muscle stem cells from DMD children were prematurely aged. In general, cell preparations with low or decreasing content in desmin-positive cells produced more MPDs than age-matched high-desmin preparations and upon implantation more human DNA and more nonmyogenic than myogenic tissue. Thus, a “Desmin Factor” was derived which predicts “quality” of the human muscle tissue growing in vivo. This factor may serve as a prognostic tool.
Oslo University College, Faculty of Nursing places undergraduate students in peer learning partne... more Oslo University College, Faculty of Nursing places undergraduate students in peer learning partnerships: 3rd year students as tutors, and 1st year students as tutees during practical skill learning. A study was designed to investigate this peer learning project. One of the research questions in particular on the role of the tutors: How do 3rd year nursing students carry out their role as supervisors for 1st year students in the skills laboratory? The study had an exploratory design. Qualitative data was gathered by three members of a research team, using video recordings of the supervised sessions and focus group interviews with both 1st and 3rd year students. Data collection was repeated in 2006e2007e2008 in different groups of students. Four identified themes illustrate how the 3rd year students supervise during the performance of the procedures in the skills laboratory:
Background: The postnatal development of the epididymis is a complex process that results in a hi... more Background: The postnatal development of the epididymis is a complex process that results in a highly differentiated epithelium, divided into several segments. Recent studies indicate a role for RNA interference (RNAi) in the development of the epididymis, however, the actual requirement for RNAi has remained elusive. Here, we present the first evidence of a direct need for RNAi in the differentiation of the epididymal epithelium.
This paper reviews EU regulation on international roaming and discusses whether this will lead to... more This paper reviews EU regulation on international roaming and discusses whether this will lead to more cost orientation when setting roaming charges in Europe. First, a cost analysis for providing international roaming is presented. Then, the various proposals put forward during the debate in the EU Parliament are discussed. Finally, the issue of cost orientation is discussed.
The purification of a 6x-histidine tagged viral coat protein (L1) in expanded mode directly follo... more The purification of a 6x-histidine tagged viral coat protein (L1) in expanded mode directly following chemical extraction from the cytoplasm of Escherichia coli HMS174(DE3) is investigated. Chelating adsorbents based on the ligands 21 21
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