DESIDOC Journal of Library & Information Technology, 2011
... pp 1-49. 21. Kademani, BS; Vijai Kumar; Anil Sagar & Anil Kumar. Scientometric ... procee... more ... pp 1-49. 21. Kademani, BS; Vijai Kumar; Anil Sagar & Anil Kumar. Scientometric ... proceedings. Mrs Shalini Tewari is working as Scientific Officer B in Scientific Information Resource Division, BARC, Trombay, Mumbai, which she joined in 2000. ...
ABSTRACT In this paper we report the frequency and temperature dependence of ac conductivity and ... more ABSTRACT In this paper we report the frequency and temperature dependence of ac conductivity and dielectric studies of Se85-xTe15Inx glasses. The measurements have been made on Se85-xTe15Inx (x = 2, 6, 10, 15) in the frequency range 2 kHz to 50 kHz and temperature range 203 K and 313 K. Results indicate that dielectric dispersion occurs in this frequency and temperature range. The results have been explained on the basis of correlated Barrier Hopping (CBH) model. The dc conductivity increases with increase in temperature for all composition. The Log σdc versus 1000/T plots are almost straight lines in whole studied temperature range indicative of singly activated phenomena having single activation energy.
Background: The concept of DNA barcoding for species identification has gained considerable momen... more Background: The concept of DNA barcoding for species identification has gained considerable momentum in animals because of fairly successful species identification using cytochrome oxidase I (COI). In plants, matK and rbcL have been proposed as standard barcodes. However, barcoding in complex genera is a challenging task. Methodology and Principal Findings: We investigated the species discriminatory power of four reportedly most promising plant DNA barcoding loci (one from nuclear genome-ITS, and three from plastid genome-trnH-psbA, rbcL and matK) in species of Indian Berberis L. (Berberidaceae) and two other genera, Ficus L. (Moraceae) and Gossypium L. (Malvaceae). Berberis species were delineated using morphological characters. These characters resulted in a well resolved species tree. Applying both nucleotide distance and nucleotide character-based approaches, we found that none of the loci, either singly or in combinations, could discriminate the species of Berberis. ITS resolved all the tested species of Ficus and Gossypium and trnH-psbA resolved 82% of the tested species in Ficus. The highly regarded matK and rbcL could not resolve all the species. Finally, we employed amplified fragment length polymorphism test in species of Berberis to determine their relationships. Using ten primer pair combinations in AFLP, the data demonstrated incomplete species resolution. Further, AFLP analysis showed that there was a tendency of the Berberis accessions to cluster according to their geographic origin rather than species affiliation. Conclusions/Significance: We reconfirm the earlier reports that the concept of universal barcode in plants may not work in a number of genera. Our results also suggest that the matK and rbcL, recommended as universal barcode loci for plants, may not work in all the genera of land plants. Morphological, geographical and molecular data analyses of Indian species of Berberis suggest probable reticulate evolution and thus barcode markers may not work in this case.
Total cross sections for quenching of low-n Rydberg states of Li (n<10, l=0) in collision ... more Total cross sections for quenching of low-n Rydberg states of Li (n<10, l=0) in collision with the ground state He have been calculated in the low-energy region (v<=0.1 a.u.) by employing the molecular orbital approximation. Results for the total depopulation of the parent states, along with those for excitation and de-excitation mechanisms have been presented. Present cross sections exhibit the
Two genotypes showing differential immunity against Karnal bunt (Tilletia indica) were used to in... more Two genotypes showing differential immunity against Karnal bunt (Tilletia indica) were used to investigate the role of three members of cystatin gene family in growth stage dependent immunity in wheat (Triticum aestivum L.). Three members of cystatin gene family (WC1, WC2, and WC4) were cloned and sequenced. Analysis of sequenced data showed that there was 76-99% nucleotide and protein sequence identity between different genes of the wheat cystatin. In silico amino acid sequence analysis revealed the presence of a conserved signature pattern of residues and also the functional domains were presumed to be actively involved in imparting cysteine protease inhibition capability. The semi-quantitative and quantitative levels of these members were measured by means of RT-PCR, northern blotting, western blotting, and by ELISA techniques. The members of cystatin gene family were expressed in both resistant (HD 29) and susceptible genotypes (WH 542); however, the expression level was significantly (P \ 0.001) higher in resistant compared to susceptible genotype at all the stages of wheat spikes. The patterns of expression of WC2, WC4 were similar except in the levels in S 1 and S 2 stages as it remained constant (P [ 0.05) in contrary to WC1 family whose expression gradually increased from S v to S 2 stage. According to the intensity of the detected band in RT PCR, northern blot and western blot, WC1 family seems to be expressed more than the other gene families. The immunoassay results further showed that WC1 protein was abundantly expressed in resistant genotype and high expression was observed at the S2 stage as compared to susceptible genotype (P \ 0.001) suggesting that low level of expression of WC1 in S2 stage is responsible for KB infection. The results of the present study clearly indicate the role of cystatin gene family in differential and stage dependent immunity against KB.
... Vikrant Gupta a , Anil Kumar b * , GK Garg a & AK Rai c pages 283-293... more ... Vikrant Gupta a , Anil Kumar b * , GK Garg a & AK Rai c pages 283-293. ... Indian J. Mycol. Plant Pathol. , 5: 185187. View all references, [10]10. Agarwal, VK, Singh, A. and Verma, HS 1976. Outbreak of Karnal Bunt of Wheat. FAO Plant Prot. Bull. , 24: 99100. View all references. ...
Disease diagnostics and pathogen detection, a primary component of any crop management programme,... more Disease diagnostics and pathogen detection, a primary component of any crop management programme, is also helpful in monitoring sanitary and phyto-sanitary measures to seed quality. Molecular diagnostics based on immunological and DNA techniques can provide an efficient way for disease surveillance and disease forecasting. Karnal bunt (KB) of wheat has become a major disease of economic importance and an important issue in the international wheat trade. Due to its seed borne, soil borne and air borne nature, KB can be diagnosed in three pathogenic forms of pathogens viz. teliospores, sporidial and mycelial forms which occur in infectious or proliferative entities at different stages of the disease cycle. Suitable sampling and extraction procedures can improve the detection limits of modern molecular diagnostic methods used to test KB. For the extraction of teliospores from both infected and infested seeds, highly efficacious detergent washing techniques have been developed and extracted pathogenic entities were diagnosed by involving several species of specific primers and immuno-chemical reagents. Nucleic acid and antibody based techniques have been developed for the detection of KB pathogen in different forms. These techniques can also be successfully employed for differential diagnosis, disease surveillance of seed borne pathogens of quarantine importance and determination of teliospore load in wheat seeds. These tests can also be designed to assist in the trade of wheat, development of disease forecasting models and molecular and immuno-pathotyping of Tilletia indica isolates collected from different agro-climatic regions. Moreover, these formats could also be employed for molecular characterization of disease determinants and in comprehending their role in molecular basis of host-pathogen interaction. The present paper highlights the use of the principle of molecular diagnostics in exerting disease surveillance and quality assurances of seeds.
DESIDOC Journal of Library & Information Technology, 2012
Indian Institute of Management Ahmedabad (IIMA) is one of the oldest institutes of management and... more Indian Institute of Management Ahmedabad (IIMA) is one of the oldest institutes of management and generally accepted as one of the leading business schools in India. Established in 1961, IIMA is frequented by the industry, local and global, to recruit its future leaders. An inherently academic institution of excellence, IIMA has unfortunately not been known for traditional research output in the form of papers in journals, as is the case with many international research institutions. It is in this context that the present study aims to review the research performance of IIMA based on the papers published in journals that have been indexed in Web of Science and Scopus for the past twelve years. The authors attempt to identify the trends in research output over the period 1999 to 2010 that includes types of publications, most preferred journals, most prolific authors from IIMA, authorship pattern, and the journals most cited by the researchers from IIMA. The findings throw interesting facts like increase in number of papers being published by IIMA over the years 1999 to 2010, increase in collaborations among authors, decrease in single author publications and multi disciplinary nature of research undertaken at the Institute.
DESIDOC Journal of Library & Information Technology, 2011
... pp 1-49. 21. Kademani, BS; Vijai Kumar; Anil Sagar & Anil Kumar. Scientometric ... procee... more ... pp 1-49. 21. Kademani, BS; Vijai Kumar; Anil Sagar & Anil Kumar. Scientometric ... proceedings. Mrs Shalini Tewari is working as Scientific Officer B in Scientific Information Resource Division, BARC, Trombay, Mumbai, which she joined in 2000. ...
ABSTRACT In this paper we report the frequency and temperature dependence of ac conductivity and ... more ABSTRACT In this paper we report the frequency and temperature dependence of ac conductivity and dielectric studies of Se85-xTe15Inx glasses. The measurements have been made on Se85-xTe15Inx (x = 2, 6, 10, 15) in the frequency range 2 kHz to 50 kHz and temperature range 203 K and 313 K. Results indicate that dielectric dispersion occurs in this frequency and temperature range. The results have been explained on the basis of correlated Barrier Hopping (CBH) model. The dc conductivity increases with increase in temperature for all composition. The Log σdc versus 1000/T plots are almost straight lines in whole studied temperature range indicative of singly activated phenomena having single activation energy.
Background: The concept of DNA barcoding for species identification has gained considerable momen... more Background: The concept of DNA barcoding for species identification has gained considerable momentum in animals because of fairly successful species identification using cytochrome oxidase I (COI). In plants, matK and rbcL have been proposed as standard barcodes. However, barcoding in complex genera is a challenging task. Methodology and Principal Findings: We investigated the species discriminatory power of four reportedly most promising plant DNA barcoding loci (one from nuclear genome-ITS, and three from plastid genome-trnH-psbA, rbcL and matK) in species of Indian Berberis L. (Berberidaceae) and two other genera, Ficus L. (Moraceae) and Gossypium L. (Malvaceae). Berberis species were delineated using morphological characters. These characters resulted in a well resolved species tree. Applying both nucleotide distance and nucleotide character-based approaches, we found that none of the loci, either singly or in combinations, could discriminate the species of Berberis. ITS resolved all the tested species of Ficus and Gossypium and trnH-psbA resolved 82% of the tested species in Ficus. The highly regarded matK and rbcL could not resolve all the species. Finally, we employed amplified fragment length polymorphism test in species of Berberis to determine their relationships. Using ten primer pair combinations in AFLP, the data demonstrated incomplete species resolution. Further, AFLP analysis showed that there was a tendency of the Berberis accessions to cluster according to their geographic origin rather than species affiliation. Conclusions/Significance: We reconfirm the earlier reports that the concept of universal barcode in plants may not work in a number of genera. Our results also suggest that the matK and rbcL, recommended as universal barcode loci for plants, may not work in all the genera of land plants. Morphological, geographical and molecular data analyses of Indian species of Berberis suggest probable reticulate evolution and thus barcode markers may not work in this case.
Total cross sections for quenching of low-n Rydberg states of Li (n<10, l=0) in collision ... more Total cross sections for quenching of low-n Rydberg states of Li (n<10, l=0) in collision with the ground state He have been calculated in the low-energy region (v<=0.1 a.u.) by employing the molecular orbital approximation. Results for the total depopulation of the parent states, along with those for excitation and de-excitation mechanisms have been presented. Present cross sections exhibit the
Two genotypes showing differential immunity against Karnal bunt (Tilletia indica) were used to in... more Two genotypes showing differential immunity against Karnal bunt (Tilletia indica) were used to investigate the role of three members of cystatin gene family in growth stage dependent immunity in wheat (Triticum aestivum L.). Three members of cystatin gene family (WC1, WC2, and WC4) were cloned and sequenced. Analysis of sequenced data showed that there was 76-99% nucleotide and protein sequence identity between different genes of the wheat cystatin. In silico amino acid sequence analysis revealed the presence of a conserved signature pattern of residues and also the functional domains were presumed to be actively involved in imparting cysteine protease inhibition capability. The semi-quantitative and quantitative levels of these members were measured by means of RT-PCR, northern blotting, western blotting, and by ELISA techniques. The members of cystatin gene family were expressed in both resistant (HD 29) and susceptible genotypes (WH 542); however, the expression level was significantly (P \ 0.001) higher in resistant compared to susceptible genotype at all the stages of wheat spikes. The patterns of expression of WC2, WC4 were similar except in the levels in S 1 and S 2 stages as it remained constant (P [ 0.05) in contrary to WC1 family whose expression gradually increased from S v to S 2 stage. According to the intensity of the detected band in RT PCR, northern blot and western blot, WC1 family seems to be expressed more than the other gene families. The immunoassay results further showed that WC1 protein was abundantly expressed in resistant genotype and high expression was observed at the S2 stage as compared to susceptible genotype (P \ 0.001) suggesting that low level of expression of WC1 in S2 stage is responsible for KB infection. The results of the present study clearly indicate the role of cystatin gene family in differential and stage dependent immunity against KB.
... Vikrant Gupta a , Anil Kumar b * , GK Garg a & AK Rai c pages 283-293... more ... Vikrant Gupta a , Anil Kumar b * , GK Garg a & AK Rai c pages 283-293. ... Indian J. Mycol. Plant Pathol. , 5: 185187. View all references, [10]10. Agarwal, VK, Singh, A. and Verma, HS 1976. Outbreak of Karnal Bunt of Wheat. FAO Plant Prot. Bull. , 24: 99100. View all references. ...
Disease diagnostics and pathogen detection, a primary component of any crop management programme,... more Disease diagnostics and pathogen detection, a primary component of any crop management programme, is also helpful in monitoring sanitary and phyto-sanitary measures to seed quality. Molecular diagnostics based on immunological and DNA techniques can provide an efficient way for disease surveillance and disease forecasting. Karnal bunt (KB) of wheat has become a major disease of economic importance and an important issue in the international wheat trade. Due to its seed borne, soil borne and air borne nature, KB can be diagnosed in three pathogenic forms of pathogens viz. teliospores, sporidial and mycelial forms which occur in infectious or proliferative entities at different stages of the disease cycle. Suitable sampling and extraction procedures can improve the detection limits of modern molecular diagnostic methods used to test KB. For the extraction of teliospores from both infected and infested seeds, highly efficacious detergent washing techniques have been developed and extracted pathogenic entities were diagnosed by involving several species of specific primers and immuno-chemical reagents. Nucleic acid and antibody based techniques have been developed for the detection of KB pathogen in different forms. These techniques can also be successfully employed for differential diagnosis, disease surveillance of seed borne pathogens of quarantine importance and determination of teliospore load in wheat seeds. These tests can also be designed to assist in the trade of wheat, development of disease forecasting models and molecular and immuno-pathotyping of Tilletia indica isolates collected from different agro-climatic regions. Moreover, these formats could also be employed for molecular characterization of disease determinants and in comprehending their role in molecular basis of host-pathogen interaction. The present paper highlights the use of the principle of molecular diagnostics in exerting disease surveillance and quality assurances of seeds.
DESIDOC Journal of Library & Information Technology, 2012
Indian Institute of Management Ahmedabad (IIMA) is one of the oldest institutes of management and... more Indian Institute of Management Ahmedabad (IIMA) is one of the oldest institutes of management and generally accepted as one of the leading business schools in India. Established in 1961, IIMA is frequented by the industry, local and global, to recruit its future leaders. An inherently academic institution of excellence, IIMA has unfortunately not been known for traditional research output in the form of papers in journals, as is the case with many international research institutions. It is in this context that the present study aims to review the research performance of IIMA based on the papers published in journals that have been indexed in Web of Science and Scopus for the past twelve years. The authors attempt to identify the trends in research output over the period 1999 to 2010 that includes types of publications, most preferred journals, most prolific authors from IIMA, authorship pattern, and the journals most cited by the researchers from IIMA. The findings throw interesting facts like increase in number of papers being published by IIMA over the years 1999 to 2010, increase in collaborations among authors, decrease in single author publications and multi disciplinary nature of research undertaken at the Institute.
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