We describe two specimens of an octopod from a hydrothermal vent of the East Pacific Rise (13°N, ... more We describe two specimens of an octopod from a hydrothermal vent of the East Pacific Rise (13°N, 2640 m) collected during the French cruise HOT 96 (CNRS/IFREMER). They are two males, one immature and one mature. The diagnosis of this new genus is as follows: benthic animals with a muscular and semi-translucent body; lack of any type of chromatic elements in the skin; eye with no trace of an iris; presence of a large white body covering the eye-ball, the optic nerves and the optic lobe; digestive gland multilobulate; presence of a black bulb, here called dark swelling, in proximal third of the intestine; presence of a crop and absence of an ink sac. The new species Vulcanoctopus hydrothermalis is a small benthic animal up to 35 mm mantle length. The posterior part of the mantle is mitre-like. Arms 2.5-4 times the length of the mantle. Right arm III hectocotylized. Ligula short (9.5 % of the hectocotylized arm), lance-shaped and without transverse ridges. Calamus short (17.9% of the l...
Based on feed resources, productive parameters and economic indicators, a typology was developed ... more Based on feed resources, productive parameters and economic indicators, a typology was developed for sheep production systems and sheep farms in the Sierra Norte region of Puebla State, Mexico. A questionnaire was designed to collect quantitative data on productive parameters and calculate relevant economic indicators. Interviews were done of a sample of 100 sheep producers and responses validated by monitoring 30 sheep farms for a one-year period. Data was collected on labor, grazing areas and practices, use of crop residues, flocks of sheep, income resources, production costs and income. Descriptive statistics were estimated for productive and economic variables and the farms classified with a multivariate analysis. Factor analysis helped to identify ewe sale price, flock size and family labor as possible competitive advantages, and production costs and lamb sale price as possible disadvantages. Productive and economical aspects had variable significance among the farms (P<0.05...
Alejandro Escánez, Álvaro Roura, Rodrigo Riera, Ángel Francisco González, and Ángel Guerra (2018)... more Alejandro Escánez, Álvaro Roura, Rodrigo Riera, Ángel Francisco González, and Ángel Guerra (2018) The systematics of the comb-fin squid species is problematic and poorly resolved. In total, 53 specimens of comb-fin squids (Chtenopteryx spp.) were caught at depths ranging from 30 to 800 m off the Canary Islands (NE Atlantic Ocean). Mantle lengths of the individuals ranged from 18 to 43 mm and the sample included immature, mature male and mature female specimens. Two species of comb-fin squids, Chtenopteryx canariensis and C. sicula, were identified by combining traditional morphological characters with a molecular analysis of a fragment of the cytochrome c oxidase subunit I (COI) gene. Intra- and interspecific genetic distances and maximum likelihood tree analyses based on COI sequences available from GenBank suggest the existence of at least four species, two from the Pacific and two from the Atlantic Ocean. Our data expand the current geographic range of C. canariensis from the NE ...
To detected alterations in the productive ecosystem of theCastillero Lagoon (Caicara of the Orino... more To detected alterations in the productive ecosystem of theCastillero Lagoon (Caicara of the Orinoco, Cedeño Municipality,Bolivar State, Venezuela), results are presented for granulometricmeasurements and heavy metals concentrations: Fe,Mn, Zn, Pb and Co made in June 2001 on superface sedimentsand of the muscular tissue of several native species offish (Plasgiosium squamossimos, Pigocentrus cariba, Pheudoplastylomafasciatum, and Hypostomus spp. By using atomicabsorption spectrophotometry with an air-acetylene flame, itwas determined that the highest concentrations of metals arerepresented by manganese, zinc and lead. It was determinedthat the highest concentrations of metals are represented bymanganese, zinc and lead. In the most internal part in the lagoonthe type of predominant sediments is the mud, constitutingamong 30% and 65% of the material collected by station. Isthat those levels fall toward the mouth of the lagoon, product of the interaction of the flow / reflux currents tha...
The content of Fe, Mn, Cu, Cr, Ni, Zn, Cd and Pb in muscle tissuesof fish and crustaceans from th... more The content of Fe, Mn, Cu, Cr, Ni, Zn, Cd and Pb in muscle tissuesof fish and crustaceans from the Unare lagoon (Lagunade Unare) in the state of Anzoátegui, Venezuela, was analyzedby flame Atomic Absorption Spectrometry. The levels of thesemetals present in suspended material of the water and in sedimentswere also determined in order to detect possible impacton the ecosystem. The mean concentrations in the water werelow (0.104-0.53 μmol/L Fe; 0.004-0.06 μmol/L Mn; 0.002-0.028μmol/L Zn; 0.004-0.012 μmol/L Cr; undetectable-0.011 μmol/LNi and undectable-0.001 μmol/L Cd). In the sediments, evidencewas found of anthropogenic intervention with mean valuesin decreasing order of Mn (516.37) > Zn (127.49) > Ni(52.41) > Cr (51.69) > Cu (41.13) > Pb (29) > Cd (1.51 μg/g),all of which are greater than established levels for uncontaminatedsediments. Toxic metals were detected in the examinedorganisms, such as lead and cadmium with levels above 0.16and 0.04 μg/g, respectively, a...
The planktonic paralarval stage of cephalopods (octopus, squids and cuttlefishes) is an important... more The planktonic paralarval stage of cephalopods (octopus, squids and cuttlefishes) is an important dispersal phase, particularly of benthic species, that lasts from days to months. Cephalopod paralarvae modify their vertical position in the water in upwelling ecosystems and such behaviour influences their spatial distribution and genetic structure, but to what extent? In this work specific water masses were sampled with Lagrangian buoys in two contrasting upwelling systems (Iberian Peninsula and Morocco) of the Iberian-Canary current eastern boundary upwelling (ICC) in order to: (i) identify the cephalopod assemblage in the different upwelling systems (ii) define their planktonic dispersal patterns and (iii) analyse the effect of different dispersal patterns on genetic structure and connectivity. Cephalopod paralarvae were identified using the cytochrome c oxidase subunit I gene (COI), revealing 21 different species and F st values showed no population structure between both upwelling systems. Cephalopod species richness was two times higher in the Moroccan upwelling than in the Iberian Peninsula, with an undescribed Ancistrocheiridae species identified in Moroccan waters. Three common planktonic dispersal patterns were identified in the ICC: coastal, coastal-oceanic and oceanic. Coastal and oceanic dispersal patterns favoured spatio-temporal paralarval retention or "schooling" of different cohorts over the continental shelf and continental slope in 9 and 11 species, respectively. Such spatio-temporal retention was reflected in the complex haplotype networks and high nucleotide/haplotype diversity recorded for these two groups. The only cephalopod species displaying a coastal-oceanic dispersal pattern was Octopus vulgaris, where low nucleotide and haplotype diversity was observed. The observed decline in genetic structure resulted from the dispersal of similar cohorts within upwelling currents and upwelling filaments to the oceanic realm. Seascape analysis revealed that cephalopod paralarvae from two coastal upwelling ecosystems of the ICC display three planktonic dispersal patterns with contrasting distributions and signatures at the genetic level.
A sampling programme to understand the factors affecting zoonotic parasite presence and infection... more A sampling programme to understand the factors affecting zoonotic parasite presence and infection intensity of Atlantic European hake populations was conducted from 2013 to 2015. Commercial fish samples comprising 430 hake specimens from northern (Grand Sole) and southern (Atlantic Iberian Peninsula) fishing grounds were sampled, recorded and inspected. We also analysed 75 additional samples collected around Scotland. Parasites were microscopically identified to genus level. Fish biometric measurements were statistically evaluated as categorical predictors of parasite recruitment into fish stocks. Stable isotope composition was determined in another sample batch of 501 hakes from the NE Atlantic (Grand Sole and Galician grounds) to explore trophic indicators as potential predictors of parasite burden. Partial sequences from mitochondrial and nuclear marker genes (mtDNA cox2 and EF1 α-1 nDNA) from a set of 915 Anisakis larvae confirmed the northern stock to be parasitized by A. simplex (s.s.) while off the Iberian Peninsula hake there was a mixed infection pattern with larvae of A. simplex s.s. (67.4% of identified larvae), A. pegreffii (31.9%) and the F1hybrid (0.7%). The observed burden of Anisakis spp. larvae differed markedly between fishing areas. The presence of very high Anisakis spp. burdens in a comparatively small proportion of individual fish was well-illustrated with characteristic tensegrity grid architecture for parasite aggregation derived from a plaque-forming cell response observed in the abdominal cavity of larger fish specimens. Demographic infection values were remarkably high in Grand Sole and off the Iberian Peninsula, with 100% prevalence and mean abundances in the fillets ranging from 247 (A. simplex) in hake from Grand Sole to 60 A. simplex and 20 A. pegreffii in southern European populations. Infections were less prevalent and less intense in the hake sample from Scottish waters. Fish size was the most important factor affecting Anisakis spp. prevalence and abundance, with larger fish containing more Anisakis larvae. Anisakis abundance within individual fish was also positively correlated with the condition index although this does not rule out negative effects on the host, and with trophic level, even after fish size was accounted for. These results suggest that higher Anisakis burden is associated with a higher food intake and with eating larger prey. The risk profile for zoonotic parasites herein described for European hake populations underlines the urgent need for adopting a contingency plan that minimizes the risk exposure. Several previous studies have investigated the level of Anisakis infection in European hake. Valero et al. (2006) and Cipriani et al. (2014) both reported higher levels of Anisakis infection in hake from the Atlantic than in the Mediterranean, noting that infection levels were also higher in bigger fish. Ceballos-Mendiola et al. (2010) found that prevalence of Anisakis simples s.s. in hake caught at Little Sole Bank (northeast Atlantic) was 100%. Studies by Sharif and Negm-Eldin (2013) and Ferrer-Maza et al. (2014) both reported a positive relationship between hake size and the number of anisakid larvae present and the latter authors also suggested a negative relationship between overall metazoan parasite burden and reproduction.
First record on stranding of a live giant squid Architeuthis dux outside Japanese waters After mu... more First record on stranding of a live giant squid Architeuthis dux outside Japanese waters After much discussion on the number of species of giant squid (Guerra et al. 2006), Winkelmann et al. (2013) showed that mitochondrial DNA lacks phylogenetic structure and that nucleotide diversity is exceptionally low, suggesting either a recent population expansion or a selective sweep. These observations are consistent with the existence of a single species of giant squid, Architeuthis dux Steenstrup, 1857, and suggest that it is highly vagile, possibly dispersing through both a drifting larval stage and migration of larger individuals. Historically, records of this elusive mesopelagic species are concentrated in five regions: (1) from Newfoundland to the Gulf of Mexico; (2) Northeast Atlantic waters, at present mostly in Northern Spain; (3) Namibia and South Africa; (4) Japan; and (5) New Zealand and Southeastern Australia (Guerra et al. 2006, Roper et al. 2015, Kubodera et al. 2016). Common features of these regions are the presence of submarine canyons near the continental shelf, high productivity, mainly produced by the upwelling of cold water (4°-6°C), existence of large predators, closeness of bays that retain carcasses, and inhabited coastal areas that allow their
Under the influence of the Western Iberian upwelling system, the Iberian Atlantic coast holds imp... more Under the influence of the Western Iberian upwelling system, the Iberian Atlantic coast holds important hatcheries and recruitment areas for Octopus vulgaris. Recently identified as an octopus hatchery, the Ría de Vigo harbors an important mesozooplankton community that supports O. vulgaris paralarvae during the first days of their planktonic stage. This study represents a preliminary approach to determine the nutritional link between wild O. vulgaris hatchlings, paralarvae and their zooplankton prey in the Ría de Vigo, by analyzing their lipid class content and fatty acid profiles. The results show that octopus hatchlings are richer in structural lipids as phospholipids and cholesterol, while the zooplankton is richer in reserve lipids like triacylglycerol and waxes. Zooplankton samples are also particularly rich in C18:1n9 and 22:6n3 (DHA), that seem to be successfully incorporated by O. vulgaris paralarvae thus resulting in a distinct fatty acid profile to that of the hatchlings....
The high mortality of cephalopod early stages is the main bottleneck to grow them from paralarvae... more The high mortality of cephalopod early stages is the main bottleneck to grow them from paralarvae to adults in culture conditions, probably because the inadequacy of the diet that results in malnutrition. Since visual analysis of digestive tract contents of paralarvae provides little evidence of diet composition, the use of molecular tools, particularly next generation sequencing (NGS) platforms, offers an alternative to understand prey preferences and nutrient requirements of wild paralarvae. In this work, we aimed to determine the diet of paralarvae of the loliginid squid Alloteuthis media and to enhance the knowledge of the diet of recently hatched Octopus vulgaris paralarvae collected in different areas and seasons in an upwelling area (NW Spain). DNA from the dissected digestive glands of 32 A. media and 64 O. vulgaris paralarvae was amplified with universal primers for the mitochondrial gene COI, and specific primers targeting the mitochondrial gene 16S gene of arthropods and ...
Turkish Journal of Anesthesia and Reanimation, 2017
Droperidol is a D2 receptor antagonist currently used in Europe for preventing postoperative naus... more Droperidol is a D2 receptor antagonist currently used in Europe for preventing postoperative nausea and vomiting. It was used to perform neurolept anaesthesia in combination with fentanyl until a Food and Drug Administration (FDA) 'black box' warning restricted its use due to cardiovascular side effects in 2001. There is no literature regarding the effects of droperidol on transcranial motor evoked potentials (TcMEPs) elicited by electrical stimulation. Our aim was to report two cases of spine surgery in which TcMEPs were lost due to droperidol administration. We report the cases of a 4-year-old male with scoliosis undergoing correction and a 58-year-old woman with metastasis on the D8 vertebrae undergoing kyphosis correction. Intraoperative neurophysiological monitoring was achieved through TcMEPs and somatosensory evoked potentials (SEPs). Intraoperative neurophysiological monitoring (IONM) showed a temporal loss of TcMEPs without SEPs changes coinciding with the administration of droperidol. TcMEP stimulation parameters were changed to double train of pulses, with the aim to elicit them, obtaining responses. Five minutes after droperidol administration, TcMEPs were equal to those at baseline. Droperidol used as prophylaxis for postoperative nausea abolishes TcMEPs. Changing stimulation parameters to double train of pulses, it allows to bypass droperidol central action, achieving monitorable TcMEPs.
Anisakids are fish-borne parasites that are responsible for a large number of human infections an... more Anisakids are fish-borne parasites that are responsible for a large number of human infections and allergic reactions around the world. World health organizations and food safety authorities aim to control and prevent this emerging health problem. In the present work, a new method for the fast monitoring of these parasites is described. The strategy is divided in three steps: (i) purification of thermostable proteins from fish-borne parasites (Anisakids), (ii) in-solution HIFU trypsin digestion and (iii) monitoring of several peptide markers by parallel reaction monitoring (PRM) mass spectrometry. This methodology allows the fast detection of Anisakids in $_amp_$lt;2h. An affordable assay utilizing this methodology will facilitate testing for regulatory and safety applications. The work describes for the first time, the Protein Biomarker Discovery and the Fast Monitoring for the identification and detection of Anisakids in fishery products. The strategy is based on the purification ...
Cuadro 2. Composición química de fosfito de potasio (FOSKA) 9 Cuadro 3. Características del mater... more Cuadro 2. Composición química de fosfito de potasio (FOSKA) 9 Cuadro 3. Características del material experimental (variedad Williams) 13 Cuadro 4. Tratamientos estudiados 14 Cuadro 5. Esquema del análisis de varianza a utilizarse en el experimento 15 Cuadro 6. Promedios de los principales caracteres morfológicos de la planta de banano 23 Cuadro 7. Presupuesto parcial 24 Cuadro 8. Análisis de dominancia 25 ÍNDICE DE CUADROS DEL ANEXO Pág. Cuadro 1A. Datos de la altura de planta del hijo sucesivo (cm). Cuadro 2A. Análisis de varianza de la variable altura de la planta del hijo sucesivo (cm). Cuadro 3A. Datos del diámetro del tallo (cm). Cuadro 4A. Análisis de varianza de la variable diámetro del tallo (cm). Cuadro 5A. Datos de emisión foliar (%). Cuadro 6A. Análisis de varianza de la variable emisión foliar (%). Cuadro 7A. Datos del peso del racimo (kg). Cuadro 8A. Análisis de varianza de la variable peso del racimo (kg). xi Cuadro 9A. Datos sobre el número de manos. Cuadro 10A. Análisis de varianza de la variable número de manos. Cuadro 11A. Datos del porcentaje de rechazo. Cuadro 12 A. Análisis de varianza de la variable porcentaje de rechazo. Cuadro 13 A. Datos del ratio. Cuadro 14 A. Análisis de varianza de la variable ratio. Cuadro 15 A. Datos de la longitud de dedo (cm). Cuadro 16 A. Análisis de varianza de la variable longitud de dedo (cm). Cuadro 17 A. Datos del calibre de fruta. Cuadro 18 A. Análisis de varianza de la variable calibre de fruta.
This report summarizes current knowledge on the identification, geographical distribution, nomenc... more This report summarizes current knowledge on the identification, geographical distribution, nomenclature, taxonomy, life history, ecology, and exploitation of cephalopod species of interest to fisheries in European waters. The 17 species range from those currently of significant fishery importance and targeted in at least part of their range (Octopus vulgaris, Sepia officinalis, Loligo vulgaris, Loligo forbesii), through those regularly landed as bycatch (Todaropsis eblanae, Illex coindetii, Eledone cirrhosa, Eledone moschata, Todarodes sagittatus), to those of minor and/or local importance (Alloteuthis subulata, Alloteuthis media, Sepia orbignyana, Sepia elegans, Sepietta oweniana, Sepiola atlantica, Ommastrephes bartramii, Gonatus fabricii). The species reviews aim to provide a concise yet comprehensive account of each, while remaining distinctive from previous and recent accounts. For most of these species, taxonomic status is well resolved, exceptions being the Alloteuthis specie...
Journal of Northwest Atlantic Fishery Science, 2010
As top predators, marine mammals can provide information on the accumulation of anthropogenic tox... more As top predators, marine mammals can provide information on the accumulation of anthropogenic toxins which present the greatest risk to consumers. We assessed the impacts of persistent organic pollutants (POPs) on two cetacean species that feed on commercially important fi sh species in the eastern North Atlantic; the common dolphin (Delphinus delphis) and the harbour porpoise (Phocoena phocoena). In order to evaluate the possible long-term effects of POPs on the continued viability of these populations, we investigated their effects on reproductive activity in females, using ovarian scars as an index of reproductive activity. In harbour porpoises, high POP burdens tended to be associated with lower ovarian scar number, possibly indicating that high contaminant levels were inhibiting ovulation, or some females may go through a number of infertile ovulations prior to a successful pregnancy, birth, and survival of their fi rst offspring during early lactation. In contrast, initial results identifi ed that the common dolphins with contaminant burdens above a threshold level for adverse health effects in marine mammals (17 μg g-1 total PCBs lipid) were resting mature females, with
Journal of the Japanese Physical Therapy Association, 2014
The types and characteristics of tension-type headaches (TTH) were included in primary headaches ... more The types and characteristics of tension-type headaches (TTH) were included in primary headaches by the International Headache Society (IHS) in 2004 1). The most common tension type headaches are episodic tension headaches (ETTH), which frequency is less than 15 days per month, and chronic tension-type headaches (CTTH), which frequency is more than 15 days per month. This last type also is characterised for its episodes of pain ranging from 30 minutes to 7 days. To be considered chronic, the headache SCIENTIFIC
We describe two specimens of an octopod from a hydrothermal vent of the East Pacific Rise (13°N, ... more We describe two specimens of an octopod from a hydrothermal vent of the East Pacific Rise (13°N, 2640 m) collected during the French cruise HOT 96 (CNRS/IFREMER). They are two males, one immature and one mature. The diagnosis of this new genus is as follows: benthic animals with a muscular and semi-translucent body; lack of any type of chromatic elements in the skin; eye with no trace of an iris; presence of a large white body covering the eye-ball, the optic nerves and the optic lobe; digestive gland multilobulate; presence of a black bulb, here called dark swelling, in proximal third of the intestine; presence of a crop and absence of an ink sac. The new species Vulcanoctopus hydrothermalis is a small benthic animal up to 35 mm mantle length. The posterior part of the mantle is mitre-like. Arms 2.5-4 times the length of the mantle. Right arm III hectocotylized. Ligula short (9.5 % of the hectocotylized arm), lance-shaped and without transverse ridges. Calamus short (17.9% of the l...
Based on feed resources, productive parameters and economic indicators, a typology was developed ... more Based on feed resources, productive parameters and economic indicators, a typology was developed for sheep production systems and sheep farms in the Sierra Norte region of Puebla State, Mexico. A questionnaire was designed to collect quantitative data on productive parameters and calculate relevant economic indicators. Interviews were done of a sample of 100 sheep producers and responses validated by monitoring 30 sheep farms for a one-year period. Data was collected on labor, grazing areas and practices, use of crop residues, flocks of sheep, income resources, production costs and income. Descriptive statistics were estimated for productive and economic variables and the farms classified with a multivariate analysis. Factor analysis helped to identify ewe sale price, flock size and family labor as possible competitive advantages, and production costs and lamb sale price as possible disadvantages. Productive and economical aspects had variable significance among the farms (P<0.05...
Alejandro Escánez, Álvaro Roura, Rodrigo Riera, Ángel Francisco González, and Ángel Guerra (2018)... more Alejandro Escánez, Álvaro Roura, Rodrigo Riera, Ángel Francisco González, and Ángel Guerra (2018) The systematics of the comb-fin squid species is problematic and poorly resolved. In total, 53 specimens of comb-fin squids (Chtenopteryx spp.) were caught at depths ranging from 30 to 800 m off the Canary Islands (NE Atlantic Ocean). Mantle lengths of the individuals ranged from 18 to 43 mm and the sample included immature, mature male and mature female specimens. Two species of comb-fin squids, Chtenopteryx canariensis and C. sicula, were identified by combining traditional morphological characters with a molecular analysis of a fragment of the cytochrome c oxidase subunit I (COI) gene. Intra- and interspecific genetic distances and maximum likelihood tree analyses based on COI sequences available from GenBank suggest the existence of at least four species, two from the Pacific and two from the Atlantic Ocean. Our data expand the current geographic range of C. canariensis from the NE ...
To detected alterations in the productive ecosystem of theCastillero Lagoon (Caicara of the Orino... more To detected alterations in the productive ecosystem of theCastillero Lagoon (Caicara of the Orinoco, Cedeño Municipality,Bolivar State, Venezuela), results are presented for granulometricmeasurements and heavy metals concentrations: Fe,Mn, Zn, Pb and Co made in June 2001 on superface sedimentsand of the muscular tissue of several native species offish (Plasgiosium squamossimos, Pigocentrus cariba, Pheudoplastylomafasciatum, and Hypostomus spp. By using atomicabsorption spectrophotometry with an air-acetylene flame, itwas determined that the highest concentrations of metals arerepresented by manganese, zinc and lead. It was determinedthat the highest concentrations of metals are represented bymanganese, zinc and lead. In the most internal part in the lagoonthe type of predominant sediments is the mud, constitutingamong 30% and 65% of the material collected by station. Isthat those levels fall toward the mouth of the lagoon, product of the interaction of the flow / reflux currents tha...
The content of Fe, Mn, Cu, Cr, Ni, Zn, Cd and Pb in muscle tissuesof fish and crustaceans from th... more The content of Fe, Mn, Cu, Cr, Ni, Zn, Cd and Pb in muscle tissuesof fish and crustaceans from the Unare lagoon (Lagunade Unare) in the state of Anzoátegui, Venezuela, was analyzedby flame Atomic Absorption Spectrometry. The levels of thesemetals present in suspended material of the water and in sedimentswere also determined in order to detect possible impacton the ecosystem. The mean concentrations in the water werelow (0.104-0.53 μmol/L Fe; 0.004-0.06 μmol/L Mn; 0.002-0.028μmol/L Zn; 0.004-0.012 μmol/L Cr; undetectable-0.011 μmol/LNi and undectable-0.001 μmol/L Cd). In the sediments, evidencewas found of anthropogenic intervention with mean valuesin decreasing order of Mn (516.37) > Zn (127.49) > Ni(52.41) > Cr (51.69) > Cu (41.13) > Pb (29) > Cd (1.51 μg/g),all of which are greater than established levels for uncontaminatedsediments. Toxic metals were detected in the examinedorganisms, such as lead and cadmium with levels above 0.16and 0.04 μg/g, respectively, a...
The planktonic paralarval stage of cephalopods (octopus, squids and cuttlefishes) is an important... more The planktonic paralarval stage of cephalopods (octopus, squids and cuttlefishes) is an important dispersal phase, particularly of benthic species, that lasts from days to months. Cephalopod paralarvae modify their vertical position in the water in upwelling ecosystems and such behaviour influences their spatial distribution and genetic structure, but to what extent? In this work specific water masses were sampled with Lagrangian buoys in two contrasting upwelling systems (Iberian Peninsula and Morocco) of the Iberian-Canary current eastern boundary upwelling (ICC) in order to: (i) identify the cephalopod assemblage in the different upwelling systems (ii) define their planktonic dispersal patterns and (iii) analyse the effect of different dispersal patterns on genetic structure and connectivity. Cephalopod paralarvae were identified using the cytochrome c oxidase subunit I gene (COI), revealing 21 different species and F st values showed no population structure between both upwelling systems. Cephalopod species richness was two times higher in the Moroccan upwelling than in the Iberian Peninsula, with an undescribed Ancistrocheiridae species identified in Moroccan waters. Three common planktonic dispersal patterns were identified in the ICC: coastal, coastal-oceanic and oceanic. Coastal and oceanic dispersal patterns favoured spatio-temporal paralarval retention or "schooling" of different cohorts over the continental shelf and continental slope in 9 and 11 species, respectively. Such spatio-temporal retention was reflected in the complex haplotype networks and high nucleotide/haplotype diversity recorded for these two groups. The only cephalopod species displaying a coastal-oceanic dispersal pattern was Octopus vulgaris, where low nucleotide and haplotype diversity was observed. The observed decline in genetic structure resulted from the dispersal of similar cohorts within upwelling currents and upwelling filaments to the oceanic realm. Seascape analysis revealed that cephalopod paralarvae from two coastal upwelling ecosystems of the ICC display three planktonic dispersal patterns with contrasting distributions and signatures at the genetic level.
A sampling programme to understand the factors affecting zoonotic parasite presence and infection... more A sampling programme to understand the factors affecting zoonotic parasite presence and infection intensity of Atlantic European hake populations was conducted from 2013 to 2015. Commercial fish samples comprising 430 hake specimens from northern (Grand Sole) and southern (Atlantic Iberian Peninsula) fishing grounds were sampled, recorded and inspected. We also analysed 75 additional samples collected around Scotland. Parasites were microscopically identified to genus level. Fish biometric measurements were statistically evaluated as categorical predictors of parasite recruitment into fish stocks. Stable isotope composition was determined in another sample batch of 501 hakes from the NE Atlantic (Grand Sole and Galician grounds) to explore trophic indicators as potential predictors of parasite burden. Partial sequences from mitochondrial and nuclear marker genes (mtDNA cox2 and EF1 α-1 nDNA) from a set of 915 Anisakis larvae confirmed the northern stock to be parasitized by A. simplex (s.s.) while off the Iberian Peninsula hake there was a mixed infection pattern with larvae of A. simplex s.s. (67.4% of identified larvae), A. pegreffii (31.9%) and the F1hybrid (0.7%). The observed burden of Anisakis spp. larvae differed markedly between fishing areas. The presence of very high Anisakis spp. burdens in a comparatively small proportion of individual fish was well-illustrated with characteristic tensegrity grid architecture for parasite aggregation derived from a plaque-forming cell response observed in the abdominal cavity of larger fish specimens. Demographic infection values were remarkably high in Grand Sole and off the Iberian Peninsula, with 100% prevalence and mean abundances in the fillets ranging from 247 (A. simplex) in hake from Grand Sole to 60 A. simplex and 20 A. pegreffii in southern European populations. Infections were less prevalent and less intense in the hake sample from Scottish waters. Fish size was the most important factor affecting Anisakis spp. prevalence and abundance, with larger fish containing more Anisakis larvae. Anisakis abundance within individual fish was also positively correlated with the condition index although this does not rule out negative effects on the host, and with trophic level, even after fish size was accounted for. These results suggest that higher Anisakis burden is associated with a higher food intake and with eating larger prey. The risk profile for zoonotic parasites herein described for European hake populations underlines the urgent need for adopting a contingency plan that minimizes the risk exposure. Several previous studies have investigated the level of Anisakis infection in European hake. Valero et al. (2006) and Cipriani et al. (2014) both reported higher levels of Anisakis infection in hake from the Atlantic than in the Mediterranean, noting that infection levels were also higher in bigger fish. Ceballos-Mendiola et al. (2010) found that prevalence of Anisakis simples s.s. in hake caught at Little Sole Bank (northeast Atlantic) was 100%. Studies by Sharif and Negm-Eldin (2013) and Ferrer-Maza et al. (2014) both reported a positive relationship between hake size and the number of anisakid larvae present and the latter authors also suggested a negative relationship between overall metazoan parasite burden and reproduction.
First record on stranding of a live giant squid Architeuthis dux outside Japanese waters After mu... more First record on stranding of a live giant squid Architeuthis dux outside Japanese waters After much discussion on the number of species of giant squid (Guerra et al. 2006), Winkelmann et al. (2013) showed that mitochondrial DNA lacks phylogenetic structure and that nucleotide diversity is exceptionally low, suggesting either a recent population expansion or a selective sweep. These observations are consistent with the existence of a single species of giant squid, Architeuthis dux Steenstrup, 1857, and suggest that it is highly vagile, possibly dispersing through both a drifting larval stage and migration of larger individuals. Historically, records of this elusive mesopelagic species are concentrated in five regions: (1) from Newfoundland to the Gulf of Mexico; (2) Northeast Atlantic waters, at present mostly in Northern Spain; (3) Namibia and South Africa; (4) Japan; and (5) New Zealand and Southeastern Australia (Guerra et al. 2006, Roper et al. 2015, Kubodera et al. 2016). Common features of these regions are the presence of submarine canyons near the continental shelf, high productivity, mainly produced by the upwelling of cold water (4°-6°C), existence of large predators, closeness of bays that retain carcasses, and inhabited coastal areas that allow their
Under the influence of the Western Iberian upwelling system, the Iberian Atlantic coast holds imp... more Under the influence of the Western Iberian upwelling system, the Iberian Atlantic coast holds important hatcheries and recruitment areas for Octopus vulgaris. Recently identified as an octopus hatchery, the Ría de Vigo harbors an important mesozooplankton community that supports O. vulgaris paralarvae during the first days of their planktonic stage. This study represents a preliminary approach to determine the nutritional link between wild O. vulgaris hatchlings, paralarvae and their zooplankton prey in the Ría de Vigo, by analyzing their lipid class content and fatty acid profiles. The results show that octopus hatchlings are richer in structural lipids as phospholipids and cholesterol, while the zooplankton is richer in reserve lipids like triacylglycerol and waxes. Zooplankton samples are also particularly rich in C18:1n9 and 22:6n3 (DHA), that seem to be successfully incorporated by O. vulgaris paralarvae thus resulting in a distinct fatty acid profile to that of the hatchlings....
The high mortality of cephalopod early stages is the main bottleneck to grow them from paralarvae... more The high mortality of cephalopod early stages is the main bottleneck to grow them from paralarvae to adults in culture conditions, probably because the inadequacy of the diet that results in malnutrition. Since visual analysis of digestive tract contents of paralarvae provides little evidence of diet composition, the use of molecular tools, particularly next generation sequencing (NGS) platforms, offers an alternative to understand prey preferences and nutrient requirements of wild paralarvae. In this work, we aimed to determine the diet of paralarvae of the loliginid squid Alloteuthis media and to enhance the knowledge of the diet of recently hatched Octopus vulgaris paralarvae collected in different areas and seasons in an upwelling area (NW Spain). DNA from the dissected digestive glands of 32 A. media and 64 O. vulgaris paralarvae was amplified with universal primers for the mitochondrial gene COI, and specific primers targeting the mitochondrial gene 16S gene of arthropods and ...
Turkish Journal of Anesthesia and Reanimation, 2017
Droperidol is a D2 receptor antagonist currently used in Europe for preventing postoperative naus... more Droperidol is a D2 receptor antagonist currently used in Europe for preventing postoperative nausea and vomiting. It was used to perform neurolept anaesthesia in combination with fentanyl until a Food and Drug Administration (FDA) 'black box' warning restricted its use due to cardiovascular side effects in 2001. There is no literature regarding the effects of droperidol on transcranial motor evoked potentials (TcMEPs) elicited by electrical stimulation. Our aim was to report two cases of spine surgery in which TcMEPs were lost due to droperidol administration. We report the cases of a 4-year-old male with scoliosis undergoing correction and a 58-year-old woman with metastasis on the D8 vertebrae undergoing kyphosis correction. Intraoperative neurophysiological monitoring was achieved through TcMEPs and somatosensory evoked potentials (SEPs). Intraoperative neurophysiological monitoring (IONM) showed a temporal loss of TcMEPs without SEPs changes coinciding with the administration of droperidol. TcMEP stimulation parameters were changed to double train of pulses, with the aim to elicit them, obtaining responses. Five minutes after droperidol administration, TcMEPs were equal to those at baseline. Droperidol used as prophylaxis for postoperative nausea abolishes TcMEPs. Changing stimulation parameters to double train of pulses, it allows to bypass droperidol central action, achieving monitorable TcMEPs.
Anisakids are fish-borne parasites that are responsible for a large number of human infections an... more Anisakids are fish-borne parasites that are responsible for a large number of human infections and allergic reactions around the world. World health organizations and food safety authorities aim to control and prevent this emerging health problem. In the present work, a new method for the fast monitoring of these parasites is described. The strategy is divided in three steps: (i) purification of thermostable proteins from fish-borne parasites (Anisakids), (ii) in-solution HIFU trypsin digestion and (iii) monitoring of several peptide markers by parallel reaction monitoring (PRM) mass spectrometry. This methodology allows the fast detection of Anisakids in $_amp_$lt;2h. An affordable assay utilizing this methodology will facilitate testing for regulatory and safety applications. The work describes for the first time, the Protein Biomarker Discovery and the Fast Monitoring for the identification and detection of Anisakids in fishery products. The strategy is based on the purification ...
Cuadro 2. Composición química de fosfito de potasio (FOSKA) 9 Cuadro 3. Características del mater... more Cuadro 2. Composición química de fosfito de potasio (FOSKA) 9 Cuadro 3. Características del material experimental (variedad Williams) 13 Cuadro 4. Tratamientos estudiados 14 Cuadro 5. Esquema del análisis de varianza a utilizarse en el experimento 15 Cuadro 6. Promedios de los principales caracteres morfológicos de la planta de banano 23 Cuadro 7. Presupuesto parcial 24 Cuadro 8. Análisis de dominancia 25 ÍNDICE DE CUADROS DEL ANEXO Pág. Cuadro 1A. Datos de la altura de planta del hijo sucesivo (cm). Cuadro 2A. Análisis de varianza de la variable altura de la planta del hijo sucesivo (cm). Cuadro 3A. Datos del diámetro del tallo (cm). Cuadro 4A. Análisis de varianza de la variable diámetro del tallo (cm). Cuadro 5A. Datos de emisión foliar (%). Cuadro 6A. Análisis de varianza de la variable emisión foliar (%). Cuadro 7A. Datos del peso del racimo (kg). Cuadro 8A. Análisis de varianza de la variable peso del racimo (kg). xi Cuadro 9A. Datos sobre el número de manos. Cuadro 10A. Análisis de varianza de la variable número de manos. Cuadro 11A. Datos del porcentaje de rechazo. Cuadro 12 A. Análisis de varianza de la variable porcentaje de rechazo. Cuadro 13 A. Datos del ratio. Cuadro 14 A. Análisis de varianza de la variable ratio. Cuadro 15 A. Datos de la longitud de dedo (cm). Cuadro 16 A. Análisis de varianza de la variable longitud de dedo (cm). Cuadro 17 A. Datos del calibre de fruta. Cuadro 18 A. Análisis de varianza de la variable calibre de fruta.
This report summarizes current knowledge on the identification, geographical distribution, nomenc... more This report summarizes current knowledge on the identification, geographical distribution, nomenclature, taxonomy, life history, ecology, and exploitation of cephalopod species of interest to fisheries in European waters. The 17 species range from those currently of significant fishery importance and targeted in at least part of their range (Octopus vulgaris, Sepia officinalis, Loligo vulgaris, Loligo forbesii), through those regularly landed as bycatch (Todaropsis eblanae, Illex coindetii, Eledone cirrhosa, Eledone moschata, Todarodes sagittatus), to those of minor and/or local importance (Alloteuthis subulata, Alloteuthis media, Sepia orbignyana, Sepia elegans, Sepietta oweniana, Sepiola atlantica, Ommastrephes bartramii, Gonatus fabricii). The species reviews aim to provide a concise yet comprehensive account of each, while remaining distinctive from previous and recent accounts. For most of these species, taxonomic status is well resolved, exceptions being the Alloteuthis specie...
Journal of Northwest Atlantic Fishery Science, 2010
As top predators, marine mammals can provide information on the accumulation of anthropogenic tox... more As top predators, marine mammals can provide information on the accumulation of anthropogenic toxins which present the greatest risk to consumers. We assessed the impacts of persistent organic pollutants (POPs) on two cetacean species that feed on commercially important fi sh species in the eastern North Atlantic; the common dolphin (Delphinus delphis) and the harbour porpoise (Phocoena phocoena). In order to evaluate the possible long-term effects of POPs on the continued viability of these populations, we investigated their effects on reproductive activity in females, using ovarian scars as an index of reproductive activity. In harbour porpoises, high POP burdens tended to be associated with lower ovarian scar number, possibly indicating that high contaminant levels were inhibiting ovulation, or some females may go through a number of infertile ovulations prior to a successful pregnancy, birth, and survival of their fi rst offspring during early lactation. In contrast, initial results identifi ed that the common dolphins with contaminant burdens above a threshold level for adverse health effects in marine mammals (17 μg g-1 total PCBs lipid) were resting mature females, with
Journal of the Japanese Physical Therapy Association, 2014
The types and characteristics of tension-type headaches (TTH) were included in primary headaches ... more The types and characteristics of tension-type headaches (TTH) were included in primary headaches by the International Headache Society (IHS) in 2004 1). The most common tension type headaches are episodic tension headaches (ETTH), which frequency is less than 15 days per month, and chronic tension-type headaches (CTTH), which frequency is more than 15 days per month. This last type also is characterised for its episodes of pain ranging from 30 minutes to 7 days. To be considered chronic, the headache SCIENTIFIC
Uploads
Papers by Angel Gonzalez