Although influenza A virus is endemic in wild waterfowl, domestic poultry, swine, humans, bats, c... more Although influenza A virus is endemic in wild waterfowl, domestic poultry, swine, humans, bats, cetaceans, dogs, and horses, there is a paucity of data on the potential role of camels in zoonotic transmission of the virus. To estimate the seroprevalence of the influenza A virus in camel populations, four local government areas of Nigeria that share an international border with the Niger Republic were selected. Blood samples from 184 one-hump camels (dromedaries) were collected and tested for influenza IgG antigen by ELISA. Each camel’s demographic variable, such as age, gender, location, production system, and usage, was recorded. The overall seroprevalence rate of influenza virus IgG in this study was 10.33% (95%CI: 6.33–15.66%). In the bivariate model, there was no significant difference in gender, age, site location and production system, except for usage. There was a significantly lower seroprevalence rate among camels used for labour (odds ratio (OR) = 0.34, 95% CI: 0.10–0.97) ...
Crimean-Congo hemorrhagic fever (CCHF) is a vector-borne viral hemorrhagic disease with global cl... more Crimean-Congo hemorrhagic fever (CCHF) is a vector-borne viral hemorrhagic disease with global clinical significance. Certain species of ticks are vectors of CCHF, which can be transmitted from animals to humans and humans to humans by direct exposure to blood or other body fluids. The zoonotic transmission at the human–animal interface from viremic animal hosts to humans is a public health concern with a paucity of data in Nigeria. Samples from 184 pastoral cattle from three local government areas (LGAs) of Plateau state, Nigeria, were screened for CCHF virus using a commercial enzyme-linked immunosorbent assay (ID Screen® CCHF Double Antigen for Multi-Species). Overall seropositivity of 30.4% (n = 56) (95% CI: 23.88%, 37.63%) was recorded from the study areas in Plateau State, while 48/126 (38.1%, 95% CI: 29.59%, 47.17%) sampled cows tested positive for CCHFV antibodies. Seropositivity was significantly higher (p < 0.001) among older cattle greater than two years, 54.69% (95% C...
Japanese encephalitis virus (JEV) is an arboviral, encephalitogenic, zoonotic flavivirus characte... more Japanese encephalitis virus (JEV) is an arboviral, encephalitogenic, zoonotic flavivirus characterized by its complex epidemiology whose transmission cycle involves reservoir and amplifying hosts, competent vector species and optimal environmental conditions. Although typically endemic in Asia and parts of the Pacific Islands, unprecedented outbreaks in both humans and domestic pigs in southeastern Australia emphasize the virus’ expanding geographical range. To estimate areas at highest risk of JEV transmission in Australia, ecological niche models of vectors and waterbirds, a sample of piggery coordinates and feral pig population density models were combined using mathematical and geospatial mapping techniques. These results highlight that both coastal and inland regions across the continent are estimated to have varying risks of enzootic and/or epidemic JEV transmission. We recommend increased surveillance of waterbirds, feral pigs and mosquito populations in areas where domestic ...
Dengue virus (DENV) is a leading mosquito-borne virus with a wide geographical spread and a major... more Dengue virus (DENV) is a leading mosquito-borne virus with a wide geographical spread and a major public health concern. DENV serotype 1 (DENV-1) and serotype 2 (DENV-2) were first reported in Africa in 1964 in Ibadan, Nigeria. Although the burden of dengue is unknown in many African countries, DENV-2 is responsible for major epidemics. In this study, we investigated the activities of DENV-2 to determine the circulating strains and to appraise the changing dynamics in the epidemiology of the virus in Nigeria. Nineteen DENV-2 sequences from 1966–2019 in Nigeria were retrieved from the GenBank of the National Center of Biotechnology Information (NCBI). A DENV genotyping tool was used to identify the specific genotypes. The evolutionary history procedure was performed on 54 DENV-2 sequences using MEGA 7. There is a deviation from Sylvatic DENV-2 to other genotypes in Nigeria. In 2019, the Asian I genotype of DENV-2 was predominant in southern Edo State, located in the tropical rainfore...
Recent Japanese encephalitis virus (JEV) outbreaks in southeastern Australia have sparked interes... more Recent Japanese encephalitis virus (JEV) outbreaks in southeastern Australia have sparked interest into epidemiological factors surrounding the virus’ novel emergence in this region. Here, the geographic distribution of mosquito species known to be competent JEV vectors in the country was estimated by combining known mosquito occurrences and ecological drivers of distribution to reveal insights into communities at highest risk of infectious disease transmission. Species distribution models predicted that Culex annulirostris and Culex sitiens presence was mostly likely along Australia’s eastern and northern coastline, while Culex quinquefasciatus presence was estimated to be most likely near inland regions of southern Australia as well as coastal regions of Western Australia. While Culex annulirostris is considered the dominant JEV vector in Australia, our ecological niche models emphasise the need for further entomological surveillance and JEV research within Australia.
The rise of bat-associated zoonotic viruses necessitates a close monitoring of their natural host... more The rise of bat-associated zoonotic viruses necessitates a close monitoring of their natural hosts. Since the detection of severe acute respiratory syndrome coronavirus (SARS-CoV), it is evident that bats are vital reservoirs of coronaviruses (CoVs). In this study, we investigated the presence of CoVs in multiple bat species in Nigeria to identify viruses in bats at high-risk human contact interfaces. Four hundred and nine bats comprising four bat species close to human habitats were individually sampled from five states in Nigeria between 2019 and 2021. Coronavirus detection was done using broadly reactive consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene of CoVs. Coronavirus RNA was detected in 39 samples (9.5%, CI 95%: [7.0, 12.8]), of which 29 were successfully sequenced. The identified CoVs in Nigerian bats were from the unclassified African alphacoronavirus lineage and betacoronavirus lineage D (Nobecovirus), with one sample from Hipposideros ruber c...
Hendra virus and Nipah virus are considered to be emerging viruses and cause severe zoonotic dise... more Hendra virus and Nipah virus are considered to be emerging viruses and cause severe zoonotic diseases, which occur in humans who have had close contact with horses and pigs in Australia and Asia, respectively. Both viruses belong to the genus Henipavirus. Although there are large populations of horses and pigs in northern Nigeria, no previous studies in this region have investigated henipavirus sero-surveillance in horses and pigs using the gold standard test, the serum neutralization test (SNT). A total of 536 apparently healthy horses and 508 apparently healthy pigs were sampled in northern Nigeria in 2018. Serum samples were tested for Hendra virus and Nipah virus-specific antibodies using either the Henipavirus Luminex binding assays for horses or the Hendra virus Competitive ELISA and Nipah virus Indirect ELISA for pigs as initial screening tests, followed by the confirmatory Hendra and Nipah virus SNT for both species, according to accredited protocols at the Australian Centre...
Rift Valley fever is an arboviral zoonoses causing severe morbidity and mortality among humans an... more Rift Valley fever is an arboviral zoonoses causing severe morbidity and mortality among humans and animals in many African countries. A cross-sectional study in populations of sheep reared around the Gidan-Waya Forest Reserve located in Jema’a LGA of Kaduna State, Nigeria to determine the serological evidence of exposure to Rift Valley fever virus (RVFV) using a commercial competitive enzyme-linked immunosorbent assay. Of the 200 sheep sampled, 9 (4.5%; 95 CI 2.23–8.33) were positive for antibodies to the RVFV. The detection of antibodies suggests a covert circulation among the sheep and may be indicative of a subclinical infection.
BackgroundRift Valley fever (RVF) is a zoonotic disease that has become emerging and re-emerging ... more BackgroundRift Valley fever (RVF) is a zoonotic disease that has become emerging and re-emerging in some regions of the world, infecting livestock and humans. One-humped camels are important economic livestock species in Africa used for traction, transportation, and food. Regional and international trade has continued to increase the risk of this disease, spreading widely and causing severe economic and public health catastrophes in affected regions. In spite of these risks, there is a dearth of information about the status of RVF in camels in Nigeria. This study was carried out to determine the prevalence of the RVF virus in one-humped camels in Nigeria and identify the risk factors associated with the disease.MethodsA cross-sectional study with simple random sampling was carried out in seven local government areas of Jigawa and Katsina States. The sera from camels were tested for anti-RVFV IgG. Camel owners were administered a structured questionnaire to ascertain their knowledge,...
Increased animal intensification has led to increased antimicrobial usage (AMU) with consequent a... more Increased animal intensification has led to increased antimicrobial usage (AMU) with consequent antimicrobial resistance (AMR) development. This study assess farmers’ practices regarding AMU in beef cattle farms, explore pathways for AMR dissemination, establish risk status of AMU, and determine residues presence. We hypothesized socio-economic factors not to drive antimicrobial misuse and resistance emergence in beef production systems. Cross-sectional study was conducted on randomly selected beef farms in Northern Nigeria between 2018 and 2019. Traffic Light model was used to assess farms’ AMU risk status. Disc Diffusion Test was performed to detect residues in cattle urine. Descriptive and multivariable logistic regressions analyses were performed at 95% confidence level. Of 660 beef cattle farmers selected, 92.1% (n=608) participated in the study. About 78.9% intensive and 76.6% semi-intensive farmers do not followed instructions on antimicrobial dosage. Also, 72.4% of intensive...
Equine piroplasmosis, an economically important disease of equids caused by the hemoprotozoan par... more Equine piroplasmosis, an economically important disease of equids caused by the hemoprotozoan parasites Theileria equi, T. haneyi, and Babesia caballi, has a worldwide distribution. These parasites are transmitted by ixodid ticks. To improve the detection of horses in Nigeria exposed to piroplasm parasites, 72 horses with variable clinical signs of piroplasmosis were sampled from Northwest and Northcentral Nigeria and tested by nPCR and cELISA. Blood and serum samples were collected from each horse via jugular venesection. Individually, nPCR or cELISA failed to identify all horses exposed to piroplasms. A combination of species-specific nPCR and the OIE-approved T. equi and B. caballi cELISAs enhanced the detection of horses exposed to parasites. The results also demonstrated horses showing abnormal hematology were positive for only T. equi, except for one sample that was coinfected with T. equi and T. haneyi. We also identified ticks collected from some of the horses, with Rhipicep...
BACKGROUND This study aimed to evaluate the prevalence of anti-West Nile virus (WNV) neutralizing... more BACKGROUND This study aimed to evaluate the prevalence of anti-West Nile virus (WNV) neutralizing antibodies in donkeys from two areas in northern Nigeria. METHODS Serology was determined by a virus neutralization test in samples collected from 205 healthy adult donkeys. RESULTS Fifty-seven donkeys (27.8%) tested seropositive for WNV. Donkeys from Zaria were 2.6 times more likely to have been exposed to WNV (p<0.001). CONCLUSION The results of this study demonstrate that this zoonotic pathogen is prevalent in these areas and that measures should be implemented to reduce the risk for both humans and equids.
Background Hypertension is a leading cause of mortality and morbidity worldwide. Hypertensives ha... more Background Hypertension is a leading cause of mortality and morbidity worldwide. Hypertensives have been observed to have elevated hearing thresholds in various studies. Aims and objectives The aims of this study were to assess the hearing thresholds of hypertensives in a tertiary hospital in Nigeria and compare it with nonhypertensive controls. Patients and methods This was a cross-sectional study conducted among hypertensives and age-matched and sex-matched controls from August 2015 to April 2016. A pretested questionnaire was used to obtain information on demographic and medical history. General physical examination and blood pressure measurements were done. Hearing thresholds were then measured with a diagnostic pure tone audiometer. Data analysis The pure tone average was calculated. Data analysis was done using the Statistical Package for Social Sciences, version 23. Results A total number of 104 participants were enrolled into the study. Fifty-two were hypertensives while the...
Background: A large-scale outbreak of meningococcal meningitis, serotype C, occurred from Decembe... more Background: A large-scale outbreak of meningococcal meningitis, serotype C, occurred from December 2016 to June 2017 in some states in Nigeria. Meningitis is associated with significant morbidity and mortality. The most common sequel of this disease is hearing loss. The mechanism could be through spread of infection to the ear via the cochlear duct. Objective: The study objective was to assess the hearing threshold of patients diagnosed with meningitis during an outbreak of meningococcal meningitis in Sokoto. Materials and Methods: This was a cross-sectional descriptive study carried out on patients diagnosed with meningitis managed at the meningitis emergency response center in Sokoto. A standardized semi-structured questionnaire was used to obtain relevant information after which the patients were examined and their hearing thresholds were measured. Results: A total of 72 patients were recruited in the study. Twenty-eight (38.9%) patients had varying degrees of hearing loss. The m...
Resistance to last resort drugs such as carbapenem and colistin is a serious global health threat... more Resistance to last resort drugs such as carbapenem and colistin is a serious global health threat. This study investigated carbapenem and colistin resistance in 583 non-duplicate Enterobacteriaceae isolates utilizing phenotypic methods and whole genome sequencing (WGS). Of the 583 isolates recovered from humans, animals and the environment in Nigeria, 18.9% (110/583) were resistant to at least one carbapenem (meropenem, ertapenem, and imipenem) and 9.1% (53/583) exhibited concurrent carbapenem-colistin resistance. The minimum inhibitory concentrations of carbapenem and colistin were 2–32 μg/mL and 8 to >64 μg/mL, respectively. No carbapenem resistant isolates produced carbapenemase nor harbored any known carbapenemase producing genes. WGS supported that concurrent carbapenem-colistin resistance was mediated by novel and previously described alterations in chromosomal efflux regulatory genes, particularly mgrB (M1V) ompC (M1_V24del) ompK37 (I70M, I128M) ramR (M1V), and marR (M1V)....
Resistance to last resort drugs such as carbapenem and colistin is a serious global health threat... more Resistance to last resort drugs such as carbapenem and colistin is a serious global health threat. This study investigated carbapenem and colistin resistance in 583 non-duplicate Enterobacteriaceae isolates utilizing phenotypic methods and whole genome sequencing (WGS). Of the 583 isolates recovered from humans, animals and the environment in Nigeria, 18.9% (110/583) were resistant to at least one carbapenem (meropenem, ertapenem, and imipenem) and 9.1% (53/583) exhibited concurrent carbapenem-colistin resistance. The minimum inhibitory concentrations of carbapenem and colistin were 2–32 μg/mL and 8 to >64 μg/mL, respectively. No carbapenem resistant isolates produced carbapenemase nor harbored any known carbapenemase producing genes. WGS supported that concurrent carbapenem-colistin resistance was mediated by novel and previously described alterations in chromosomal efflux regulatory genes, particularly mgrB (M1V) ompC (M1_V24del) ompK37 (I70M, I128M) ramR (M1V), and marR (M1V)....
Although influenza A virus is endemic in wild waterfowl, domestic poultry, swine, humans, bats, c... more Although influenza A virus is endemic in wild waterfowl, domestic poultry, swine, humans, bats, cetaceans, dogs, and horses, there is a paucity of data on the potential role of camels in zoonotic transmission of the virus. To estimate the seroprevalence of the influenza A virus in camel populations, four local government areas of Nigeria that share an international border with the Niger Republic were selected. Blood samples from 184 one-hump camels (dromedaries) were collected and tested for influenza IgG antigen by ELISA. Each camel’s demographic variable, such as age, gender, location, production system, and usage, was recorded. The overall seroprevalence rate of influenza virus IgG in this study was 10.33% (95%CI: 6.33–15.66%). In the bivariate model, there was no significant difference in gender, age, site location and production system, except for usage. There was a significantly lower seroprevalence rate among camels used for labour (odds ratio (OR) = 0.34, 95% CI: 0.10–0.97) ...
Crimean-Congo hemorrhagic fever (CCHF) is a vector-borne viral hemorrhagic disease with global cl... more Crimean-Congo hemorrhagic fever (CCHF) is a vector-borne viral hemorrhagic disease with global clinical significance. Certain species of ticks are vectors of CCHF, which can be transmitted from animals to humans and humans to humans by direct exposure to blood or other body fluids. The zoonotic transmission at the human–animal interface from viremic animal hosts to humans is a public health concern with a paucity of data in Nigeria. Samples from 184 pastoral cattle from three local government areas (LGAs) of Plateau state, Nigeria, were screened for CCHF virus using a commercial enzyme-linked immunosorbent assay (ID Screen® CCHF Double Antigen for Multi-Species). Overall seropositivity of 30.4% (n = 56) (95% CI: 23.88%, 37.63%) was recorded from the study areas in Plateau State, while 48/126 (38.1%, 95% CI: 29.59%, 47.17%) sampled cows tested positive for CCHFV antibodies. Seropositivity was significantly higher (p < 0.001) among older cattle greater than two years, 54.69% (95% C...
Japanese encephalitis virus (JEV) is an arboviral, encephalitogenic, zoonotic flavivirus characte... more Japanese encephalitis virus (JEV) is an arboviral, encephalitogenic, zoonotic flavivirus characterized by its complex epidemiology whose transmission cycle involves reservoir and amplifying hosts, competent vector species and optimal environmental conditions. Although typically endemic in Asia and parts of the Pacific Islands, unprecedented outbreaks in both humans and domestic pigs in southeastern Australia emphasize the virus’ expanding geographical range. To estimate areas at highest risk of JEV transmission in Australia, ecological niche models of vectors and waterbirds, a sample of piggery coordinates and feral pig population density models were combined using mathematical and geospatial mapping techniques. These results highlight that both coastal and inland regions across the continent are estimated to have varying risks of enzootic and/or epidemic JEV transmission. We recommend increased surveillance of waterbirds, feral pigs and mosquito populations in areas where domestic ...
Dengue virus (DENV) is a leading mosquito-borne virus with a wide geographical spread and a major... more Dengue virus (DENV) is a leading mosquito-borne virus with a wide geographical spread and a major public health concern. DENV serotype 1 (DENV-1) and serotype 2 (DENV-2) were first reported in Africa in 1964 in Ibadan, Nigeria. Although the burden of dengue is unknown in many African countries, DENV-2 is responsible for major epidemics. In this study, we investigated the activities of DENV-2 to determine the circulating strains and to appraise the changing dynamics in the epidemiology of the virus in Nigeria. Nineteen DENV-2 sequences from 1966–2019 in Nigeria were retrieved from the GenBank of the National Center of Biotechnology Information (NCBI). A DENV genotyping tool was used to identify the specific genotypes. The evolutionary history procedure was performed on 54 DENV-2 sequences using MEGA 7. There is a deviation from Sylvatic DENV-2 to other genotypes in Nigeria. In 2019, the Asian I genotype of DENV-2 was predominant in southern Edo State, located in the tropical rainfore...
Recent Japanese encephalitis virus (JEV) outbreaks in southeastern Australia have sparked interes... more Recent Japanese encephalitis virus (JEV) outbreaks in southeastern Australia have sparked interest into epidemiological factors surrounding the virus’ novel emergence in this region. Here, the geographic distribution of mosquito species known to be competent JEV vectors in the country was estimated by combining known mosquito occurrences and ecological drivers of distribution to reveal insights into communities at highest risk of infectious disease transmission. Species distribution models predicted that Culex annulirostris and Culex sitiens presence was mostly likely along Australia’s eastern and northern coastline, while Culex quinquefasciatus presence was estimated to be most likely near inland regions of southern Australia as well as coastal regions of Western Australia. While Culex annulirostris is considered the dominant JEV vector in Australia, our ecological niche models emphasise the need for further entomological surveillance and JEV research within Australia.
The rise of bat-associated zoonotic viruses necessitates a close monitoring of their natural host... more The rise of bat-associated zoonotic viruses necessitates a close monitoring of their natural hosts. Since the detection of severe acute respiratory syndrome coronavirus (SARS-CoV), it is evident that bats are vital reservoirs of coronaviruses (CoVs). In this study, we investigated the presence of CoVs in multiple bat species in Nigeria to identify viruses in bats at high-risk human contact interfaces. Four hundred and nine bats comprising four bat species close to human habitats were individually sampled from five states in Nigeria between 2019 and 2021. Coronavirus detection was done using broadly reactive consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene of CoVs. Coronavirus RNA was detected in 39 samples (9.5%, CI 95%: [7.0, 12.8]), of which 29 were successfully sequenced. The identified CoVs in Nigerian bats were from the unclassified African alphacoronavirus lineage and betacoronavirus lineage D (Nobecovirus), with one sample from Hipposideros ruber c...
Hendra virus and Nipah virus are considered to be emerging viruses and cause severe zoonotic dise... more Hendra virus and Nipah virus are considered to be emerging viruses and cause severe zoonotic diseases, which occur in humans who have had close contact with horses and pigs in Australia and Asia, respectively. Both viruses belong to the genus Henipavirus. Although there are large populations of horses and pigs in northern Nigeria, no previous studies in this region have investigated henipavirus sero-surveillance in horses and pigs using the gold standard test, the serum neutralization test (SNT). A total of 536 apparently healthy horses and 508 apparently healthy pigs were sampled in northern Nigeria in 2018. Serum samples were tested for Hendra virus and Nipah virus-specific antibodies using either the Henipavirus Luminex binding assays for horses or the Hendra virus Competitive ELISA and Nipah virus Indirect ELISA for pigs as initial screening tests, followed by the confirmatory Hendra and Nipah virus SNT for both species, according to accredited protocols at the Australian Centre...
Rift Valley fever is an arboviral zoonoses causing severe morbidity and mortality among humans an... more Rift Valley fever is an arboviral zoonoses causing severe morbidity and mortality among humans and animals in many African countries. A cross-sectional study in populations of sheep reared around the Gidan-Waya Forest Reserve located in Jema’a LGA of Kaduna State, Nigeria to determine the serological evidence of exposure to Rift Valley fever virus (RVFV) using a commercial competitive enzyme-linked immunosorbent assay. Of the 200 sheep sampled, 9 (4.5%; 95 CI 2.23–8.33) were positive for antibodies to the RVFV. The detection of antibodies suggests a covert circulation among the sheep and may be indicative of a subclinical infection.
BackgroundRift Valley fever (RVF) is a zoonotic disease that has become emerging and re-emerging ... more BackgroundRift Valley fever (RVF) is a zoonotic disease that has become emerging and re-emerging in some regions of the world, infecting livestock and humans. One-humped camels are important economic livestock species in Africa used for traction, transportation, and food. Regional and international trade has continued to increase the risk of this disease, spreading widely and causing severe economic and public health catastrophes in affected regions. In spite of these risks, there is a dearth of information about the status of RVF in camels in Nigeria. This study was carried out to determine the prevalence of the RVF virus in one-humped camels in Nigeria and identify the risk factors associated with the disease.MethodsA cross-sectional study with simple random sampling was carried out in seven local government areas of Jigawa and Katsina States. The sera from camels were tested for anti-RVFV IgG. Camel owners were administered a structured questionnaire to ascertain their knowledge,...
Increased animal intensification has led to increased antimicrobial usage (AMU) with consequent a... more Increased animal intensification has led to increased antimicrobial usage (AMU) with consequent antimicrobial resistance (AMR) development. This study assess farmers’ practices regarding AMU in beef cattle farms, explore pathways for AMR dissemination, establish risk status of AMU, and determine residues presence. We hypothesized socio-economic factors not to drive antimicrobial misuse and resistance emergence in beef production systems. Cross-sectional study was conducted on randomly selected beef farms in Northern Nigeria between 2018 and 2019. Traffic Light model was used to assess farms’ AMU risk status. Disc Diffusion Test was performed to detect residues in cattle urine. Descriptive and multivariable logistic regressions analyses were performed at 95% confidence level. Of 660 beef cattle farmers selected, 92.1% (n=608) participated in the study. About 78.9% intensive and 76.6% semi-intensive farmers do not followed instructions on antimicrobial dosage. Also, 72.4% of intensive...
Equine piroplasmosis, an economically important disease of equids caused by the hemoprotozoan par... more Equine piroplasmosis, an economically important disease of equids caused by the hemoprotozoan parasites Theileria equi, T. haneyi, and Babesia caballi, has a worldwide distribution. These parasites are transmitted by ixodid ticks. To improve the detection of horses in Nigeria exposed to piroplasm parasites, 72 horses with variable clinical signs of piroplasmosis were sampled from Northwest and Northcentral Nigeria and tested by nPCR and cELISA. Blood and serum samples were collected from each horse via jugular venesection. Individually, nPCR or cELISA failed to identify all horses exposed to piroplasms. A combination of species-specific nPCR and the OIE-approved T. equi and B. caballi cELISAs enhanced the detection of horses exposed to parasites. The results also demonstrated horses showing abnormal hematology were positive for only T. equi, except for one sample that was coinfected with T. equi and T. haneyi. We also identified ticks collected from some of the horses, with Rhipicep...
BACKGROUND This study aimed to evaluate the prevalence of anti-West Nile virus (WNV) neutralizing... more BACKGROUND This study aimed to evaluate the prevalence of anti-West Nile virus (WNV) neutralizing antibodies in donkeys from two areas in northern Nigeria. METHODS Serology was determined by a virus neutralization test in samples collected from 205 healthy adult donkeys. RESULTS Fifty-seven donkeys (27.8%) tested seropositive for WNV. Donkeys from Zaria were 2.6 times more likely to have been exposed to WNV (p<0.001). CONCLUSION The results of this study demonstrate that this zoonotic pathogen is prevalent in these areas and that measures should be implemented to reduce the risk for both humans and equids.
Background Hypertension is a leading cause of mortality and morbidity worldwide. Hypertensives ha... more Background Hypertension is a leading cause of mortality and morbidity worldwide. Hypertensives have been observed to have elevated hearing thresholds in various studies. Aims and objectives The aims of this study were to assess the hearing thresholds of hypertensives in a tertiary hospital in Nigeria and compare it with nonhypertensive controls. Patients and methods This was a cross-sectional study conducted among hypertensives and age-matched and sex-matched controls from August 2015 to April 2016. A pretested questionnaire was used to obtain information on demographic and medical history. General physical examination and blood pressure measurements were done. Hearing thresholds were then measured with a diagnostic pure tone audiometer. Data analysis The pure tone average was calculated. Data analysis was done using the Statistical Package for Social Sciences, version 23. Results A total number of 104 participants were enrolled into the study. Fifty-two were hypertensives while the...
Background: A large-scale outbreak of meningococcal meningitis, serotype C, occurred from Decembe... more Background: A large-scale outbreak of meningococcal meningitis, serotype C, occurred from December 2016 to June 2017 in some states in Nigeria. Meningitis is associated with significant morbidity and mortality. The most common sequel of this disease is hearing loss. The mechanism could be through spread of infection to the ear via the cochlear duct. Objective: The study objective was to assess the hearing threshold of patients diagnosed with meningitis during an outbreak of meningococcal meningitis in Sokoto. Materials and Methods: This was a cross-sectional descriptive study carried out on patients diagnosed with meningitis managed at the meningitis emergency response center in Sokoto. A standardized semi-structured questionnaire was used to obtain relevant information after which the patients were examined and their hearing thresholds were measured. Results: A total of 72 patients were recruited in the study. Twenty-eight (38.9%) patients had varying degrees of hearing loss. The m...
Resistance to last resort drugs such as carbapenem and colistin is a serious global health threat... more Resistance to last resort drugs such as carbapenem and colistin is a serious global health threat. This study investigated carbapenem and colistin resistance in 583 non-duplicate Enterobacteriaceae isolates utilizing phenotypic methods and whole genome sequencing (WGS). Of the 583 isolates recovered from humans, animals and the environment in Nigeria, 18.9% (110/583) were resistant to at least one carbapenem (meropenem, ertapenem, and imipenem) and 9.1% (53/583) exhibited concurrent carbapenem-colistin resistance. The minimum inhibitory concentrations of carbapenem and colistin were 2–32 μg/mL and 8 to >64 μg/mL, respectively. No carbapenem resistant isolates produced carbapenemase nor harbored any known carbapenemase producing genes. WGS supported that concurrent carbapenem-colistin resistance was mediated by novel and previously described alterations in chromosomal efflux regulatory genes, particularly mgrB (M1V) ompC (M1_V24del) ompK37 (I70M, I128M) ramR (M1V), and marR (M1V)....
Resistance to last resort drugs such as carbapenem and colistin is a serious global health threat... more Resistance to last resort drugs such as carbapenem and colistin is a serious global health threat. This study investigated carbapenem and colistin resistance in 583 non-duplicate Enterobacteriaceae isolates utilizing phenotypic methods and whole genome sequencing (WGS). Of the 583 isolates recovered from humans, animals and the environment in Nigeria, 18.9% (110/583) were resistant to at least one carbapenem (meropenem, ertapenem, and imipenem) and 9.1% (53/583) exhibited concurrent carbapenem-colistin resistance. The minimum inhibitory concentrations of carbapenem and colistin were 2–32 μg/mL and 8 to >64 μg/mL, respectively. No carbapenem resistant isolates produced carbapenemase nor harbored any known carbapenemase producing genes. WGS supported that concurrent carbapenem-colistin resistance was mediated by novel and previously described alterations in chromosomal efflux regulatory genes, particularly mgrB (M1V) ompC (M1_V24del) ompK37 (I70M, I128M) ramR (M1V), and marR (M1V)....
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