Papers by Andreani Odysseos
In the vast field of nanomedicine, "Theranostics" combine therapeutics and diagnostics, aiming to... more In the vast field of nanomedicine, "Theranostics" combine therapeutics and diagnostics, aiming to provide a comprehensive platform for diagnosis, therapy and monitoring of the patient, leading to customized approaches and personalized treatment. Emerging nanotechnology discoveries provide a unique opportunity to design and develop such combination agents, permitting the delivery of therapeutics and concurrently allowing the detection modality to be used not only before or after but also throughout the entire treatment regimen, defining new supra-disciplinary fields in major clinical specialties such as Radiology, Surgery, Neurology and Oncology, to mention few. A Nanotheranostics Conference is an important initiative, expected to provide the forum for idea exchange and create a potential high-impact nanomedicine paradigm. Inspired by the success of ICoN2013-the 1 st International Conference of Nanotheranostics, ICoN 2015 aims to provide the optimal venue to expand nanotheranostics research in a multidisciplinary environment which will bring together all the key researchers in the field. ICoN2015 has attracted the contribution of prominent entities in the Nanomedicine field and has succeeded in bringing together high-caliber researchers and a promising population of young scientists at the dawn of their career from all five continents. It combines thoughtfully topic-oriented plenary and keynote lectures with innovative research papers. Major thematic areas include (i) the roadmap of nanotheranostics development in a patient-oriented approach, (ii) emerging challenges for nanotheranostic applications, (iii) nanoscience technologies for theranostic approaches, (iv) an emphasis session on cancer nanotheranostics, (v) toxicology, regulatory issues and ethics. Specially designated sessions on (i) breaking through cellular and biological barriers and (b) facing challenges in thermotherapy are reflecting translational challenges under EC-funded schemes. Featuring two specially designated events, the "Meet with the Professor Session", wholeheartedly devoted to the younger generation of Theranostics scientists and a Clinically-oriented seminar opening horizons towards clinical translation, ICoN2015 is expected to set the grounds for the establishment of a lasting inter-sectoral and transdisciplinary network on novel diagnostics and therapeutics both locally and on an international dimension. Cyprus, an island of nano-scale dimensions from a global prospective, but with tremendous potentials when it comes to creativity and effectiveness, is certainly the place for a Nanotheranostics Conference to be! Limassol, the "Princess of Mediterranean" has attracted and assimilated onto its Hellenic trunk, cultures from around the world, has hosted Saints and Crusadors and is now waiting to serve you its splendid flavor. Surrounded by prehistoric , Hellenistic, Roman and Byzantine monuments the city of wine is laying for you to discover. I wholeheartedly wish that you make the most of ICoN2015 and take home a unique scientific and cultural experience.
The definitive version is available at www.blackwell-synergy.com © Federation of European Biochem... more The definitive version is available at www.blackwell-synergy.com © Federation of European Biochemical Societies
IEEE Transactions on Nanobioscience, Jul 1, 2021
Glioblastoma Multiforme (GBM), the most malignant human tumour, can be defined by the evolution o... more Glioblastoma Multiforme (GBM), the most malignant human tumour, can be defined by the evolution of growing bio-nanomachine networks within an interplay between self-renewal (Grow) and invasion (Go) potential of mutually exclusive phenotypes of transmitter and receiver cells. Herein, we present a mathematical model for the growth of GBM tumour driven by molecule-mediated inter-cellular communication between two populations of evolutionary bio-nanomachines representing the Glioma Stem Cells (GSCs) and Glioma Cells (GCs). The contribution of each subpopulation to tumour growth is quantified by a voxel model representing the end to end inter-cellular communication models for GSCs and progressively evolving invasiveness levels of glioma cells within a network of diverse cell configurations. Mutual information, information propagation speed and the impact of cell numbers and phenotypes on the communication output and GBM growth are studied by using analysis from information theory. The numerical simulations show that the progression of GBM is directly related to higher mutual information and higher input information flow of molecules between the GSCs and GCs, resulting in an increased tumour growth rate. These fundamental findings contribute to deciphering the mechanisms of tumour growth and are expected to provide new knowledge towards the development of future bio-nanomachine-based therapeutic approaches for GBM.
ChemInform, Mar 14, 2014
The reactivity of the Δ7,10-double bond of bicyclic[3.3.1]nonatriones such as (I) is investigated... more The reactivity of the Δ7,10-double bond of bicyclic[3.3.1]nonatriones such as (I) is investigated resulting in the discovery of a novel skeletal rearrangement to a highly functionalized bicyclo[3.3.0]octane.
Organic Letters, Oct 11, 2013
A novel skeletal rearrangement of bicyclo[3.3.1]nonane-2,4,9-trione (16) to an unprecedented high... more A novel skeletal rearrangement of bicyclo[3.3.1]nonane-2,4,9-trione (16) to an unprecedented highly functionalized bicyclo[3.3.0]octane system (17), induced by an intramolecular Michael addition, is presented. This novel framework was found to be similarly active to hyperforin (1), against PC-3 cell lines. A mechanistic study was examined in detail, proposing a number of cascade transformations. Also, reactivity of the Δ 7,10-double bond was examined under several conditions to explain the above results.
Advances in Experimental Medicine and Biology, 1991
The CD4 and CD8 antigens on the surface of T cells appear to bind to major histocompatibility com... more The CD4 and CD8 antigens on the surface of T cells appear to bind to major histocompatibility complex (MHC) class II and I antigens, respectively. These antigens also synergize with the Ti(TcR)/CD3 complex in the potentiation of T-cell proliferation. Our earlier work demonstrated that the CD4 and CD8 receptors are coupled to a protein-tyrosine kinase termed p56lck from normal and transformed T lymphocytes. The p56lck protein is a member of the src family and its homology with receptor-kinases such as the epidermal growth factor receptor (EGFR) make it an important candidate in signal transduction. In this paper, we show in transfectants that p56lck interacts with the cytoplasmic tail of the CD4 antigen. Murine p56lck can interact across species with the human CD4 receptor. Furthermore, peptide competition studies showed that a specific sequence within the cytoplasmic tail of CD4 interacts with the kinase. Cysteine residues also appear to play key roles in this interaction. Lastly, we show biochemically that the CD4:p56lck complex can physically associate with the epsilon chain of the CD3 complex on HPB-ALL transformed T cells. This interaction may provide a bridge by which events related to ligand binding to Ti(TcR)/CD3 may trigger T cells via the CD4/CD8:p56lck complex.
Bioorganic & Medicinal Chemistry, Apr 1, 2006
Vitamin E succinate selenium-conjugated molecules were synthesized and their apoptogenic properti... more Vitamin E succinate selenium-conjugated molecules were synthesized and their apoptogenic properties were evaluated. 4-Methyl-2-phenylselenyl succinate (4) was prepared by the reaction of sodium benzeneselenolate with 2-bromosuccinic anhydrite in methanol solution. The methyl ester was converted to the acid (5) by hydrolysis with aqueous hydrochloric acid. Reaction of the 2-phenylselenyl succinic anhydrite (6) with a-tocopherol (1a), c-tocopherol (1c), and c-tocotrienol (2c) in acidic conditions gave the respective esters. The free radical scavenging properties of a-tocopheryl-2-phenylselenyl succinate (7), c-tocopheryl-2-phenylselenyl succinate (8), and c-tocotrienyl-2-phenylselenyl succinate (9) were evaluated in comparison with those of a-tocopheryl succinate (10), c-tocopheryl succinate (11), and c-tocotrienyl succinate (12), respectively, and the free tocopherols and c-tocotrienol. Compounds 7-9 induced a statistically significant decrease in prostate cancer cell viability compared to 10-12, respectively, or 5, exhibiting features of apoptotic cell death and associated with caspase-3 activation. These data show that structural modifications of vitamin E components by 5 enhance their apoptogenic properties in cancer cells.
Molecular Pharmaceutics, Sep 11, 2019
Chemical Synthesis Procedures and Characterization Data 7-Nitroquinazolin-4(3H)-one (3). To a rou... more Chemical Synthesis Procedures and Characterization Data 7-Nitroquinazolin-4(3H)-one (3). To a round-bottom flask were transferred 2-amino-4-nitrobenzoic acid (1) (10.00 g, 55 mmol, 1 equiv) and formamidine acetate (2) (11.45 g, 110 mmol, 2 equiv). The flask was fitted with a vertical condenser and set under argon atmosphere. Methoxyethanol (55 mL) was added and the mixture was refluxed at 130 o C for 18 h. It was then cooled down to r.t. The resulting precipitate was filtered, washed several times with aqueous ammonia (1.5 L x 0.01 M) and with DCM, then allowed to dry under vacuum, to yield 9.75 g (51 mmol, 93%) of compound 3, as a beige powder. 1
Journal of Biomedical Materials Research Part B, Aug 9, 2012
The mechanical properties and hydraulic permeabilities of biomaterial scaffolds play a crucial ro... more The mechanical properties and hydraulic permeabilities of biomaterial scaffolds play a crucial role in their efficacy as tissue engineering platforms, separation processors, and drug delivery vehicles. In this study, electrospun cellulose acetate fiber meshes of random orientations were created using four different concentrations, 10.0, 12.5, 15.0, and 17.5 wt % in acetone or ethyl acetate. The tensile mechanical properties and the hydraulic permeabilities of these meshes were measured, and a multiscale model was employed to predict their mechanical behavior. Experimentally, the elastic modulus ranged from 3.5 to 12.4 MPa depending on the polymer concentration and the solvent. Model predictions agreed well with the experimental measurements when a fitted single-fiber modulus of 123.3 MPa was used. The model also predicted that changes in fiber alignment may result in a 3.6-fold increase in the elastic modulus for moderately aligned meshes and a 8.5-fold increase for highly align meshes. Hydraulic permeabilities ranged from 1.4 x 10 À12 to 8.9 x 10 À12 m 2 depending on polymer concentration but not the choice of solvent. In conclusion, polymer concentration, fiber alignment, and solvent have significant impact on the mechanical and fluid transport properties of electrospun cellulose acetate fiber meshes. V
International Journal of Obesity, Feb 17, 2009
Objectives: Cross-sectional data suggest a strong association between low levels of physical acti... more Objectives: Cross-sectional data suggest a strong association between low levels of physical activity and obesity. The EPIC-PANACEA (European Prospective Investigation into Cancer-Physical Activity, Nutrition, Alcohol, Cessation of Smoking, Eating out of home And obesity) project was designed to investigate the associations between physical activity and body mass index (BMI) and waist circumference based on individual data collected across nine European countries. Methods: In the European Prospective Investigation into Cancer and Nutrition (EPIC), 519 931 volunteers were recruited between 1992 and 2000, of whom 405 819 had data on main variables of interest. Height, body weight and waist circumference were measured using standardized procedures. Physical activity was assessed using a validated four-category
European Journal of Immunology, Jul 1, 1991
The CD4 and CD8 antigens function in synergy with the TcR/CD3 complex in the generation of intrac... more The CD4 and CD8 antigens function in synergy with the TcR/CD3 complex in the generation of intracellular signals leading to T cell proliferation. The association of the protein‐tyrosine kinase p56lck with CD4 and CD8 provides a potential mechanism in the generation of intracellular signals. Several studies have shown that CD4 can co‐modulate with TcR/CD3 suggesting that these receptor complexes may associate on the surface of the T cell. Nevertheless, it has proven difficult to formally demonstrate a direct physical interaction between the CD4 and TcR/CD3 complexes using biochemical techniques. In this study, we have used the sensitivity of the in vitro kinase assay to show a direct physical linkage between the CD4: p56lck complex and various CD3 subunits. Immunoprecipitation of CD4 from cell lysates derived from the T lymphoblastoid line HPB‐ALL results in the co‐purification of p56lck with an additional polypeptide at 20 kDa. Re‐precipitation analysis and isoelectric focusing demonstrated that this band corresponds to the CD3ϵ chain. An alternative approach which involves the labeling of microsomal membranes with [γ−32P]ATP revealed the presence of CD3ϵ and ζ chains in anti‐CD4 immunoprecipitates. By contrast, we were unable to demonstrate the association of the CD4: p56lck and TcR/CD3 complex in resting peripheral blood lymphocytes. These data indicate that the CD4: p56lck and TcR/CD3 complexes have the ability to form stable complexes on the surface of certain T cell lines.
bioRxiv (Cold Spring Harbor Laboratory), Apr 18, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
PLOS ONE, Aug 11, 2014
Mechanical forces play a crucial role in tumor patho-physiology. Compression of cancer cells inhi... more Mechanical forces play a crucial role in tumor patho-physiology. Compression of cancer cells inhibits their proliferation rate, induces apoptosis and enhances their invasive and metastatic potential. Additionally, compression of intratumor blood vessels reduces the supply of oxygen, nutrients and drugs, affecting tumor progression and treatment. Despite the great importance of the mechanical microenvironment to the pathology of cancer, there are limited studies for the constitutive modeling and the mechanical properties of tumors and on how these parameters affect tumor growth. Also, the contribution of the host tissue to the growth and state of stress of the tumor remains unclear. To this end, we performed unconfined compression experiments in two tumor types and found that the experimental stress-strain response is better fitted to an exponential constitutive equation compared to the widely used neo-Hookean and Blatz-Ko models. Subsequently, we incorporated the constitutive equations along with the corresponding values of the mechanical properties-calculated by the fit-to a biomechanical model of tumor growth. Interestingly, we found that the evolution of stress and the growth rate of the tumor are independent from the selection of the constitutive equation, but depend strongly on the mechanical interactions with the surrounding host tissue. Particularly, model predictions-in agreement with experimental studies-suggest that the stiffness of solid tumors should exceed a critical value compared with that of the surrounding tissue in order to be able to displace the tissue and grow in size. With the use of the model, we estimated this critical value to be on the order of 1.5. Our results suggest that the direct effect of solid stress on tumor growth involves not only the inhibitory effect of stress on cancer cell proliferation and the induction of apoptosis, but also the resistance of the surrounding tissue to tumor expansion.
Journal of Nutrition, Sep 1, 2009
Given the lack of consistent evidence of the relationship between Mediterranean dietary patterns ... more Given the lack of consistent evidence of the relationship between Mediterranean dietary patterns and body fat, we assessed the cross-sectional association between adherence to a modified Mediterranean diet, BMI, and waist
In this paper, we propose a mathematical and computational model for the GBM initiating of recurr... more In this paper, we propose a mathematical and computational model for the GBM initiating of recurring focus as a quasi-spherical absorbing receiver considering the irregular shape as a Bernoulli trial process that accounts for the uncontrollable tumor growth over an initial spherical surface. Our results show that when GBM grow to irregular quasi-sphere shapes, they will increase the channel capacity, which is fully aligned with the evolution and configuration of GSC niches in GBM cultures. CCS CONCEPTS • Mathematics of computing → Information theory; • Applied computing → Systems biology; • Computing methodologies → Modeling and simulation.
Glioblastoma Multiform (GBM) is known as one of the most malignant tumours in the brain, and chal... more Glioblastoma Multiform (GBM) is known as one of the most malignant tumours in the brain, and challenges remain in developing effective therapeutic solutions. This paper addresses an open-loop control molecular communication system using an adaptive algorithm that controls engineered induced Neural Stem Cells (iNSCs) to release therapeutic exosomes for treating GBM. The adaptive algorithm is based on the Lotka-Volterra Predator-Prey model, and virtually monitors the tumour growth from an external Brain-Machine Interface to control the release of the exosomes for the treatment. We developed the model to incorporate the control from an external RF signal that controls the production of exosomes as well as the diffusion propagation of exosomes through a 3D simulated Extracellular Space tissue. Based on numerical analysis coupled with simulations, we found that factors such as stochastic propagation of exosomes influence the aggressiveness of the model to tackle the tumour. This work can lay the foundation for future adaptive Brain-Machine Interface that controls molecular communication system for GBM treatment.
Bone, May 1, 2011
The aim of this study was evaluation of osteoinductive properties of demineralized bovine foetal ... more The aim of this study was evaluation of osteoinductive properties of demineralized bovine foetal growth plate in submuscular transplantation (ectopic osteoinduction). Demineralized bovine foetal growth plate was ectopically implanted in 18 male Sprague-Dawley rats. In 18 of the animals under aseptic conditions two submuscular pouches were created between external and internal oblique abdominal muscles in the two flanks: the right was left empty (sham) and the left was filled with 20 mg of demineralized bovine foetal growth plate powder. Radiographs were taken in 2, 4 and 6 weeks after the surgery, then six animals were pharmacologically euthanized after 2, 4 and 6 weeks for histopathological evaluation. Results showed: (1) osteoinductivity of xenogenic demineralized bovine foetal growth plate powder, and (2) earlier mineralization of ectopically implanted demineralized bovine foetal growth plate in the submuscular implanted area. Our results show that submuscular implantation of xenogenic demineralized bovine foetal growth plate has osteoinductive properties in a rat model.
Networks of bio-nanomachines that communicate through molecular communication are expected to per... more Networks of bio-nanomachines that communicate through molecular communication are expected to perform complex functionalities within biological systems. The natural history of malignant tumors can be defined by the evolution of growing bio-nanomachine networks within an interplay between proliferation or self-renewal (Grow) and invasion (Go) potential of mutually exclusive phenotypes. Herein we present a model of two populations of bio-nanomachines representing distinct phenotypes propagating throughout the progression of malignant gliomas within spatiotemporally evolving bionanomachine networks, driven by either attractive and linkforming or repulsive and cluster-forming forces. This model is further applied in computer simulations to examine the growth of bio-nanomachine networks in terms of size and network connectivity. Understanding the mechanisms by which malignant cells form bio-nanomachine networks and controlling network connectivity can contribute to deciphering mechanisms of tumor evolution and progression and provide new nanonetwork-based therapeutic approaches.
IEEE Transactions on Molecular, Biological and Multi-Scale Communications
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Papers by Andreani Odysseos