Background: Artificial separation of oral mucosa into epithelium and connective tissue represents... more Background: Artificial separation of oral mucosa into epithelium and connective tissue represents the first step in investigating both the basement membrane zone and its two components (epithelium and connective tissue) separately. It is a useful tool in analysing the possible role of hepatitis C virus in the pathogenesis of oral lichen planus (OLP). The aim of the present study was to test different methods to separate the epithelium from the connective tissue of oral mucosa affected by OLP as a preliminary step for further immunohistochemical or molecular investigations. Patients and methods: Fourteen tissue biopsies of the oral mucosa of clinically and histopathologically confirmed OLP lesions underwent epithelial connective separation (ECS) using six different reagents. Results and conclusions: Among the separation methods tested, CaCl 2 1 M at 37˚C for 30 min, NaCl 1 M at 4˚C for 26 h and elastase at 4˚C for 22 h allowed good ECS without structural alteration or tissue loss. The present study provides useful information regarding techniques to separate the epithelium from the connective tissue as a preliminary step to investigate oral mucosa affected by OLP from an immunohistochemical or molecular viewpoint. Bez C, Moneghini L, Nicali A, Cazzaniga A, Lodi G, Bosari S, Sardella A, Carrassi A. Characterization of induced mucosal connective tissue separation-a comparison of six different techniques.
Riassunto Obiettivi Descrivere un nuovo protocollo per la consegna di un’overdenture definitiva, ... more Riassunto Obiettivi Descrivere un nuovo protocollo per la consegna di un’overdenture definitiva, su impianti a carico immediato e con pilastri conici personalizzati, senza eseguire la fase di impronta degli impianti. Materiali e metodi E stato trattato il mascellare superiore edentulo di una paziente portatrice di protesi totale mobile. Dopo l’acquisizione di una tomografia computerizzata a fascio conico (Cone Beam Computed Tomography, CBCT) e di due impronte studio, il caso e stato elaborato con il software per chirurgia guidata Simplant (Dentsply IH, Molndal, Svezia). Tramite la dima chirurgica Simplant e stato ricavato il modello master modificando il modello ottenuto dall’impronta studio in alginato. Sono stati quindi richiesti i pilastri protesici personalizzati Atlantis (Dentsply IH) (pilastri conici con angolo di 5°) e si e realizzata la protesi definitiva in laboratorio. Al secondo appuntamento sono stati inseriti quattro impianti Astra Tech EV (Dentsply IH) in maniera guidata e flapless, avvitati i pilastri definitivi ed e stata consegnata la protesi overdenture a carico immediato. Risultati Nella stessa seduta sono stati inseriti gli impianti e si e applicata la protesi definitiva, senza complicanze. Conclusioni La procedura eseguita ha limitato notevolmente il tempo speso alla poltrona e il caso e stato realizzato in maniera mininvasiva e predicibile. Le tecniche e i materiali utilizzati godono di una documentazione scientifica consistente e di una certa versatilita.
Articolo originale ITALIAN ORAL SURGERY 2012;11(5)(S1):S74-S80 | S74 Riassunto Obiettivi : Discut... more Articolo originale ITALIAN ORAL SURGERY 2012;11(5)(S1):S74-S80 | S74 Riassunto Obiettivi : Discutere la gestione di interventi chirurgici a livello dei dotti salivari descrivendo il trattamento di uno pseudofi broma traumatico in corrispondenza del dotto di Stenone, un mucocele a livello della ghiandola sottolinguale e una sialolitiasi del pavimento orale. Materiali e metodi : l'incannulazione del dotto, la sialodocoplastica e la marsupializzazione della lesione. Grazie a queste procedure è possibile risolvere la maggior parte delle più comuni patologie legate all'ostruzione del fl usso salivare. Risultati e conclusioni : Un'attenta programmazione chirurgica e l'adozione di alcuni accorgimenti tecnici permettono di risolvere con semplicità i problemi clinici dei pazienti affetti da queste condizioni e di prevenire complicanze legate alla prossimità di strutture delicate.
Adenomatoid odontogenic tumor (AOT) is a benign, slow-growing lesion and it is considered the fou... more Adenomatoid odontogenic tumor (AOT) is a benign, slow-growing lesion and it is considered the fourth most common odontogenic tumor. AOT affects more frequently the young subjects with a predilection for the second and third decades of life. We present here the case of a 36-year-old man affected by large extrafollicular AOT which caused roots resorption of multiple teeth. The lesion involved the entire right maxillary bone and extended into the right maxillary sinus up to the orbital floor and the nasal cavity. Intra-oral surgical excision of the AOT and functional endoscopic sinus surgery led to complete clinical healing in absence of local recurrences.
Riassunto Obiettivi Descrivere un nuovo protocollo per la consegna di un’overdenture definitiva, ... more Riassunto Obiettivi Descrivere un nuovo protocollo per la consegna di un’overdenture definitiva, su impianti a carico immediato e con pilastri conici personalizzati, senza eseguire la fase di impronta degli impianti. Materiali e metodi E stato trattato il mascellare superiore edentulo di una paziente portatrice di protesi totale mobile. Dopo l’acquisizione di una tomografia computerizzata a fascio conico (Cone Beam Computed Tomography, CBCT) e di due impronte studio, il caso e stato elaborato con il software per chirurgia guidata Simplant (Dentsply IH, Molndal, Svezia). Tramite la dima chirurgica Simplant e stato ricavato il modello master modificando il modello ottenuto dall’impronta studio in alginato. Sono stati quindi richiesti i pilastri protesici personalizzati Atlantis (Dentsply IH) (pilastri conici con angolo di 5°) e si e realizzata la protesi definitiva in laboratorio. Al secondo appuntamento sono stati inseriti quattro impianti Astra Tech EV (Dentsply IH) in maniera guidata e flapless, avvitati i pilastri definitivi ed e stata consegnata la protesi overdenture a carico immediato. Risultati Nella stessa seduta sono stati inseriti gli impianti e si e applicata la protesi definitiva, senza complicanze. Conclusioni La procedura eseguita ha limitato notevolmente il tempo speso alla poltrona e il caso e stato realizzato in maniera mininvasiva e predicibile. Le tecniche e i materiali utilizzati godono di una documentazione scientifica consistente e di una certa versatilita.
Objectives: Despite a large amount of materials and methods to make an implantsupported denture, ... more Objectives: Despite a large amount of materials and methods to make an implantsupported denture, nowadays there is no gold standard. Every solution has pros and cons that guide the clinician and the technician to choose the best solution for a single case. The aim of this study was to evaluate the mechanical characteristics of the fiber-reinforced composite superstructure made by using a novel threedimensional (3D) printing technology able to create a reinforcing structure patient-specific, more reliable, structurally optimized, and faster than conventional methods. Materials and Methods: To evaluate mechanical performances of 3D-printed fiberglass, mechanical characterization of 3D-printed material was performed. Before proceeding with the realization of the final prosthesis, five specimens were created on which the tensile test and volumetric fiber content measurement were performed. Then denture reinforcement 3D printing process began. Initially, the robot prints layers of fiber. Finally, the obtained 3D-printed reinforcement structure was finalized in the lab. Results: The prosthesis obtained through this process was lighter than a traditional prosthesis, there was a greater chemical adhesion between resin and 3D-printed reinforcement structure and a better result was obtained from an esthetic point of view. Conclusions: The outcomes we obtained endorse its performance both mechanical and esthetic. The entire process is automatic and does not require human operation thanks to specific software programming.
Parole chiave: * Terapia antitrombotica * Doppia terapia antiaggregante * Chirurgia orale * Clopi... more Parole chiave: * Terapia antitrombotica * Doppia terapia antiaggregante * Chirurgia orale * Clopidogrel * Emorragia unnecessary oral surgery should be postponed to avoid interrupting the dual therapy. Key words: * Antithrombotic therapy * Dual antiplatelet therapy * Oral surgery * Clopidogrel * Bleeding A. Valenti et al. * Sono indicati i dosaggi giornalieri la cui efficacia e stata dimostrata da studi randomizzati.
Background: Artificial separation of oral mucosa into epithelium and connective tissue represents... more Background: Artificial separation of oral mucosa into epithelium and connective tissue represents the first step in investigating both the basement membrane zone and its two components (epithelium and connective tissue) separately. It is a useful tool in analysing the possible role of hepatitis C virus in the pathogenesis of oral lichen planus (OLP). The aim of the present study was to test different methods to separate the epithelium from the connective tissue of oral mucosa affected by OLP as a preliminary step for further immunohistochemical or molecular investigations. Patients and methods: Fourteen tissue biopsies of the oral mucosa of clinically and histopathologically confirmed OLP lesions underwent epithelial connective separation (ECS) using six different reagents. Results and conclusions: Among the separation methods tested, CaCl 2 1 M at 37˚C for 30 min, NaCl 1 M at 4˚C for 26 h and elastase at 4˚C for 22 h allowed good ECS without structural alteration or tissue loss. The present study provides useful information regarding techniques to separate the epithelium from the connective tissue as a preliminary step to investigate oral mucosa affected by OLP from an immunohistochemical or molecular viewpoint. Bez C, Moneghini L, Nicali A, Cazzaniga A, Lodi G, Bosari S, Sardella A, Carrassi A. Characterization of induced mucosal connective tissue separation-a comparison of six different techniques.
AIM The aim of the present study was to verify the possibility of obtaining an optimized prosthet... more AIM The aim of the present study was to verify the possibility of obtaining an optimized prosthetic substructure using generic software, respecting the distribution loads and forces involved. What is considered to be original and innovative in this study is the possibility of designing the prosthetic substructure on the basis of the individual patient's chewing biomechanics, with the purpose of obtaining an even greater efficiency than a prosthesis designed according to a traditional method. MATERIALS AND METHODS The starting standard triangulation language (STL) file was processed with Rhinoceros software and the tOpos plugin. It was decided to submit the entire prosthetic solution, intended as total volume, to structural analysis and topological optimization because the entire prosthesis is subjected to load during the chewing act. The software program was provided with information on the material, modulus, and direction of the applied forces. The objective was to optimize sti...
Background: Artificial separation of oral mucosa into epithelium and connective tissue represents... more Background: Artificial separation of oral mucosa into epithelium and connective tissue represents the first step in investigating both the basement membrane zone and its two components (epithelium and connective tissue) separately. It is a useful tool in analysing the possible role of hepatitis C virus in the pathogenesis of oral lichen planus (OLP). The aim of the present study was to test different methods to separate the epithelium from the connective tissue of oral mucosa affected by OLP as a preliminary step for further immunohistochemical or molecular investigations. Patients and methods: Fourteen tissue biopsies of the oral mucosa of clinically and histopathologically confirmed OLP lesions underwent epithelial connective separation (ECS) using six different reagents. Results and conclusions: Among the separation methods tested, CaCl 2 1 M at 37˚C for 30 min, NaCl 1 M at 4˚C for 26 h and elastase at 4˚C for 22 h allowed good ECS without structural alteration or tissue loss. The present study provides useful information regarding techniques to separate the epithelium from the connective tissue as a preliminary step to investigate oral mucosa affected by OLP from an immunohistochemical or molecular viewpoint. Bez C, Moneghini L, Nicali A, Cazzaniga A, Lodi G, Bosari S, Sardella A, Carrassi A. Characterization of induced mucosal connective tissue separation-a comparison of six different techniques.
Riassunto Obiettivi Descrivere un nuovo protocollo per la consegna di un’overdenture definitiva, ... more Riassunto Obiettivi Descrivere un nuovo protocollo per la consegna di un’overdenture definitiva, su impianti a carico immediato e con pilastri conici personalizzati, senza eseguire la fase di impronta degli impianti. Materiali e metodi E stato trattato il mascellare superiore edentulo di una paziente portatrice di protesi totale mobile. Dopo l’acquisizione di una tomografia computerizzata a fascio conico (Cone Beam Computed Tomography, CBCT) e di due impronte studio, il caso e stato elaborato con il software per chirurgia guidata Simplant (Dentsply IH, Molndal, Svezia). Tramite la dima chirurgica Simplant e stato ricavato il modello master modificando il modello ottenuto dall’impronta studio in alginato. Sono stati quindi richiesti i pilastri protesici personalizzati Atlantis (Dentsply IH) (pilastri conici con angolo di 5°) e si e realizzata la protesi definitiva in laboratorio. Al secondo appuntamento sono stati inseriti quattro impianti Astra Tech EV (Dentsply IH) in maniera guidata e flapless, avvitati i pilastri definitivi ed e stata consegnata la protesi overdenture a carico immediato. Risultati Nella stessa seduta sono stati inseriti gli impianti e si e applicata la protesi definitiva, senza complicanze. Conclusioni La procedura eseguita ha limitato notevolmente il tempo speso alla poltrona e il caso e stato realizzato in maniera mininvasiva e predicibile. Le tecniche e i materiali utilizzati godono di una documentazione scientifica consistente e di una certa versatilita.
Articolo originale ITALIAN ORAL SURGERY 2012;11(5)(S1):S74-S80 | S74 Riassunto Obiettivi : Discut... more Articolo originale ITALIAN ORAL SURGERY 2012;11(5)(S1):S74-S80 | S74 Riassunto Obiettivi : Discutere la gestione di interventi chirurgici a livello dei dotti salivari descrivendo il trattamento di uno pseudofi broma traumatico in corrispondenza del dotto di Stenone, un mucocele a livello della ghiandola sottolinguale e una sialolitiasi del pavimento orale. Materiali e metodi : l'incannulazione del dotto, la sialodocoplastica e la marsupializzazione della lesione. Grazie a queste procedure è possibile risolvere la maggior parte delle più comuni patologie legate all'ostruzione del fl usso salivare. Risultati e conclusioni : Un'attenta programmazione chirurgica e l'adozione di alcuni accorgimenti tecnici permettono di risolvere con semplicità i problemi clinici dei pazienti affetti da queste condizioni e di prevenire complicanze legate alla prossimità di strutture delicate.
Adenomatoid odontogenic tumor (AOT) is a benign, slow-growing lesion and it is considered the fou... more Adenomatoid odontogenic tumor (AOT) is a benign, slow-growing lesion and it is considered the fourth most common odontogenic tumor. AOT affects more frequently the young subjects with a predilection for the second and third decades of life. We present here the case of a 36-year-old man affected by large extrafollicular AOT which caused roots resorption of multiple teeth. The lesion involved the entire right maxillary bone and extended into the right maxillary sinus up to the orbital floor and the nasal cavity. Intra-oral surgical excision of the AOT and functional endoscopic sinus surgery led to complete clinical healing in absence of local recurrences.
Riassunto Obiettivi Descrivere un nuovo protocollo per la consegna di un’overdenture definitiva, ... more Riassunto Obiettivi Descrivere un nuovo protocollo per la consegna di un’overdenture definitiva, su impianti a carico immediato e con pilastri conici personalizzati, senza eseguire la fase di impronta degli impianti. Materiali e metodi E stato trattato il mascellare superiore edentulo di una paziente portatrice di protesi totale mobile. Dopo l’acquisizione di una tomografia computerizzata a fascio conico (Cone Beam Computed Tomography, CBCT) e di due impronte studio, il caso e stato elaborato con il software per chirurgia guidata Simplant (Dentsply IH, Molndal, Svezia). Tramite la dima chirurgica Simplant e stato ricavato il modello master modificando il modello ottenuto dall’impronta studio in alginato. Sono stati quindi richiesti i pilastri protesici personalizzati Atlantis (Dentsply IH) (pilastri conici con angolo di 5°) e si e realizzata la protesi definitiva in laboratorio. Al secondo appuntamento sono stati inseriti quattro impianti Astra Tech EV (Dentsply IH) in maniera guidata e flapless, avvitati i pilastri definitivi ed e stata consegnata la protesi overdenture a carico immediato. Risultati Nella stessa seduta sono stati inseriti gli impianti e si e applicata la protesi definitiva, senza complicanze. Conclusioni La procedura eseguita ha limitato notevolmente il tempo speso alla poltrona e il caso e stato realizzato in maniera mininvasiva e predicibile. Le tecniche e i materiali utilizzati godono di una documentazione scientifica consistente e di una certa versatilita.
Objectives: Despite a large amount of materials and methods to make an implantsupported denture, ... more Objectives: Despite a large amount of materials and methods to make an implantsupported denture, nowadays there is no gold standard. Every solution has pros and cons that guide the clinician and the technician to choose the best solution for a single case. The aim of this study was to evaluate the mechanical characteristics of the fiber-reinforced composite superstructure made by using a novel threedimensional (3D) printing technology able to create a reinforcing structure patient-specific, more reliable, structurally optimized, and faster than conventional methods. Materials and Methods: To evaluate mechanical performances of 3D-printed fiberglass, mechanical characterization of 3D-printed material was performed. Before proceeding with the realization of the final prosthesis, five specimens were created on which the tensile test and volumetric fiber content measurement were performed. Then denture reinforcement 3D printing process began. Initially, the robot prints layers of fiber. Finally, the obtained 3D-printed reinforcement structure was finalized in the lab. Results: The prosthesis obtained through this process was lighter than a traditional prosthesis, there was a greater chemical adhesion between resin and 3D-printed reinforcement structure and a better result was obtained from an esthetic point of view. Conclusions: The outcomes we obtained endorse its performance both mechanical and esthetic. The entire process is automatic and does not require human operation thanks to specific software programming.
Parole chiave: * Terapia antitrombotica * Doppia terapia antiaggregante * Chirurgia orale * Clopi... more Parole chiave: * Terapia antitrombotica * Doppia terapia antiaggregante * Chirurgia orale * Clopidogrel * Emorragia unnecessary oral surgery should be postponed to avoid interrupting the dual therapy. Key words: * Antithrombotic therapy * Dual antiplatelet therapy * Oral surgery * Clopidogrel * Bleeding A. Valenti et al. * Sono indicati i dosaggi giornalieri la cui efficacia e stata dimostrata da studi randomizzati.
Background: Artificial separation of oral mucosa into epithelium and connective tissue represents... more Background: Artificial separation of oral mucosa into epithelium and connective tissue represents the first step in investigating both the basement membrane zone and its two components (epithelium and connective tissue) separately. It is a useful tool in analysing the possible role of hepatitis C virus in the pathogenesis of oral lichen planus (OLP). The aim of the present study was to test different methods to separate the epithelium from the connective tissue of oral mucosa affected by OLP as a preliminary step for further immunohistochemical or molecular investigations. Patients and methods: Fourteen tissue biopsies of the oral mucosa of clinically and histopathologically confirmed OLP lesions underwent epithelial connective separation (ECS) using six different reagents. Results and conclusions: Among the separation methods tested, CaCl 2 1 M at 37˚C for 30 min, NaCl 1 M at 4˚C for 26 h and elastase at 4˚C for 22 h allowed good ECS without structural alteration or tissue loss. The present study provides useful information regarding techniques to separate the epithelium from the connective tissue as a preliminary step to investigate oral mucosa affected by OLP from an immunohistochemical or molecular viewpoint. Bez C, Moneghini L, Nicali A, Cazzaniga A, Lodi G, Bosari S, Sardella A, Carrassi A. Characterization of induced mucosal connective tissue separation-a comparison of six different techniques.
AIM The aim of the present study was to verify the possibility of obtaining an optimized prosthet... more AIM The aim of the present study was to verify the possibility of obtaining an optimized prosthetic substructure using generic software, respecting the distribution loads and forces involved. What is considered to be original and innovative in this study is the possibility of designing the prosthetic substructure on the basis of the individual patient's chewing biomechanics, with the purpose of obtaining an even greater efficiency than a prosthesis designed according to a traditional method. MATERIALS AND METHODS The starting standard triangulation language (STL) file was processed with Rhinoceros software and the tOpos plugin. It was decided to submit the entire prosthetic solution, intended as total volume, to structural analysis and topological optimization because the entire prosthesis is subjected to load during the chewing act. The software program was provided with information on the material, modulus, and direction of the applied forces. The objective was to optimize sti...
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