Papers by Andrea Dorottya Székely
Bratislava Medical Journal, 2020
Neuroscience Letters, Jun 1, 1995
Combined nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry and nitr... more Combined nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry and nitric oxide synthase (NOS) immunocytochemistry were used to study the distribution of nitric oxide synthesizing elements in the cat submandibular gland. A large number of thin varicose fibres, with intense staining for both markers, were seen around or in close contact with the acini. Some of the stained nerve fibres were associated with intra- and interlobular salivary ducts and blood vessels. All neurones in the submandibular ganglia showed intense staining for both NADPH-d and NOS. The epithelial layer of the salivary ductal branches and the endothelial lining of blood vessels were NOS immunonegative but NADPH-d positive. Our results suggest that NO might act as a neurotransmitter in the regulation of blood flow and secretion in the submandibular salivary gland.
Journal of Periodontology, Mar 1, 2016
In the present study, the possible localization and role of vascular endothelial growth factor re... more In the present study, the possible localization and role of vascular endothelial growth factor receptor type 2 (VEGFR2) in the regulation of gingival venules in a rat model of experimental diabetes are examined. Six weeks after streptozotocin premedication, Wistar male rats presenting blood sugar levels >20 mmol/L were selected for investigation. The VEGFR2 antagonist ZM323881 [5-((7-benzyloxyquinazolin-4-yl)amino)-4-fluoro-2-methylphenol-hydrochloride] (20 μg/mL) was dripped onto the gingiva between the mandibular incisors. Changes in diameter of the selected gingival venule were measured by vital microscopy combined with digital photography at specified times. Immunohistochemical staining was used to localize VEGFR2. For controls, the same protocol was used on animals with normal blood sugar levels and healthy gingiva. There was a significant difference between the baseline venule diameter of the diabetic and the control groups (47 ± 1 and 28 ± 2 μm, respectively). After 15, 30, and 60 minutes of local application of ZM323881, significant vasoconstriction was observed in the venules of diabetic rats compared with the baseline (81.4% ± 4.6%, 81.8% ± 4.4%, and 80.6% ± 5.1%, respectively). The control group showed no change in the venule diameter. The immunohistochemical analysis showed significantly increased VEGFR2 expression in the mast cells along the venules in the diabetic group, whereas mast cells were rarely found in the control group. The findings suggest that VEGF expression is increased in gingiva in experimentally induced diabetes. After VEGFR2 activation, the mast cell-derived vasodilatory and inflammatory mediators may contribute markedly to the concomitant changes in the microcirculation.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 2009
The pentadecapeptide BPC 157 has been shown to have anti-inflammatory and wound healing effects o... more The pentadecapeptide BPC 157 has been shown to have anti-inflammatory and wound healing effects on multiple target tissues and organs. The purpose of the present study was to investigate the effect of BPC 157 on inflammation and bone resorption in experimental periodontitis in rats. First the acute effect of BPC was tested on gingival blood flow by laser doppler flowmetry. Then periodontitis was produced by a silk ligature placed around the lower left first molar. Rats were treated with BPC 157 (once daily for 12 days) or vehicle. At day 13, the gingivomucosal tissues encircling the molars were removed on both sides. Inflammation was assessed by Evans blue plasma extravasation technique and by histology. Alveolar bone loss was analyzed by microCT. BPC 157 had no effect on gingivomucosal blood flow. Twelve day ligature caused a significantly increased Evans blue extravasation in the gingivomucosal tissue, histological signs of inflammation, and alveolar bone destruction. BPC 157 trea...
Clinical Oral Investigations, Jul 1, 2018
Objectives The aim of the present study is to provide a detailed macroscopic mapping of the palat... more Objectives The aim of the present study is to provide a detailed macroscopic mapping of the palatal and tuberal blood supply applying anatomical methods and studying specific anastomoses to bridge the gap between basic structural and empirical clinical knowledge. Materials and methods Ten cadavers (three dentate, seven edentulous) have been prepared for this study in the Department of Anatomy, Semmelweis University, Budapest, Hungary, and in the Department of Anatomy of the Medical University of Graz. All cadavers were fixed with Thiel's solution. For the macroscopic analysis of the blood vessels supplying the palatal mucosa, corrosion casting in four cadavers and latex milk injection in other six cadavers were performed. Results We recorded major-and secondary branches of the greater palatine artery (GPA) and its relation to the palatine spine, different anastomoses with the nasopalatine artery (NPA), and lesser palatal artery (LPA) as well as with contralateral branches of the GPA. Penetrating intraosseous branches at the premolar-canine area were also detected. In edentulous patients, the GPA developed a curvy pathway in the premolar area. The blood supply around the maxillary tuberosity was also presented. Conclusion The combination of different staining methods has shed light to findings with relevance to palatal blood supply, offering a powerful tool for the design and execution of surgical interventions involving the hard palate. Clinical relevance The present study provides clinicians with a good basis to understand the anatomical background of palatal and tuberal blood supply. This might enable clinicians to design optimized incision-and flap designs. As a result, the risk of intraoperative bleeding and postoperative wound healing complications related to impaired blood supply can be minimized.
The FASEB Journal, Apr 1, 2013
Performance enhancement and health, Aug 1, 2012
Research into the Female Athlete Triad (FAT) often posits that the condition is one of the unwant... more Research into the Female Athlete Triad (FAT) often posits that the condition is one of the unwanted consequences of increased physical activity and the prevailing preference for a lean body among female athletes; as well as the result of mounting pressure for constant performance improvement, which is often coupled to a misconception that low body weight would help to achieve this goal. This paper challenges the prevailing concept of the FAT for being inexact and over-specific, giving the impression that only athletes are affected by this condition, whilst the narrow focus on the co-occurrence of disordered eating-amenorrhea-osteoporosis can potentially lead to incorrect diagnoses of females suffering from, or at risk of developing, the condition. As the common underlying factor in athletes and non-athlete females suffering from FAT conditions is chronically low energy availability (via increased physical activity and/or disordered or restricted eating), we propose a unified framework that focuses on this common characteristic. Under the umbrella term 'Female Energy Deficiency' (FED), the expanded FAT and related concepts such as Anorexia Athletica and atypical eating disorder may be reconciled. The suggested framework can facilitate the understanding of this convoluted field within and outside the athletic community and offers flexibility for future developments. To support our proposition, we discuss the: i) expansion of the components to capture the extent and depth of this health condition, ii) expansion of the 'at risk' population, and iii) effective prevention, along with the need for early diagnosis and treatment.
Chemistry Central Journal, Dec 1, 2012
Background: Anabolic androgenic steroids, such as stanozolol, are typically misused by athletes d... more Background: Anabolic androgenic steroids, such as stanozolol, are typically misused by athletes during preparation for competition. Out-of-competition testing presents a unique challenge in the current anti-doping detection system owing to logistic reasons. Analysing hair for the presence of a prohibited drug offers a feasible solution for covering the wider window in out-of-competition testing. To assist in vivo studies aiming to establish a relationship between drug levels detected in hair, urine and blood, sensitive methods for the determination of stanozolol and its major metabolite 3 0-hydroxystanozolol were developed in pigmented hair, urine and serum, using brown Norway rats as a model system and liquid chromatography tandem mass spectrometry (LC-MS/MS). Results: For method development, spiked drug free rat hair, blood and urine samples were used. The newly developed method was then applied to hair, urine and serum samples from five brown Norway rats after treatment (intraperitoneal) with stanozolol for six consecutive days at 5.0 mg/kg/day. The assay for each matrix was linear within the quantification range with determination coefficient (r 2) values above 0.995. The respective assay was capable of detecting 0.125 pg/mg stanozolol and 0.25 pg/mg 3 0-hydroxystanozolol with 50 mg hair; 0.063 ng/mL stanozolol and 0.125 ng/mL 3 0-hydroxystanozolol with 100 μL of urine or serum. The accuracy, precision and extraction recoveries of the assays were satisfactory for the detection of both compounds in all three matrices. The average concentrations of stanozolol and 3 0-hydroxystanozolol, were as follows: hair = 70.18 ± 22.32 pg/mg and 13.01 ± 3.43 pg/mg; urine = 4.34 ± 6.54 ng/mL and 9.39 ± 7.42 ng/mL; serum = 7.75 ± 3.58 ng/mL and 7.16 ± 1.97 ng/mL, respectively. Conclusions: The developed methods are sensitive, specific and reproducible for the determination of stanozolol and 3 0-hydroxystanozolol in rat hair, urine and serum. These methods can be used for in vivo studies further investigating stanozolol metabolism, but also could be extended for doping testing. Owing to the complementary nature of these tests, with urine and serum giving information on recent drug use and hair providing retrospective information on habitual use, it is suggested that blood or urine tests could accompany hair analysis and thus avoid false doping results.
Journal of comparative neurology, Apr 8, 1993
ABSTRACT The present study is the first comprehensive mapping of glial fibrillary acidic protein ... more ABSTRACT The present study is the first comprehensive mapping of glial fibrillary acidic protein (GFAP)-immunopositive structures in the avian brain. Two main types of GFAP-immunopositive elements were observed: (1) nonbranching fibers, occasionally twisted or varicose, and (2) star-shaped cells. Long immunostained fibers orignate from the lateral ventricle to form three bundles. Fibers of the dorsal group, emanating from the dorsal/lateral corner of the ventricle, course in lateral, anterior, and ventral directions forming a semidome, which separates the outer pallial (lateral cortical) regions from the underlying striatal mass. The middle group of fibers is directed anteriorly and laterally corresponding to the laminae frontales superior and suprema. The ventral fiber bundle is conical and traverses the lobus parolfactorius, crossing also the lamina medullaris dorsalis (the latter consisting mainly of star-shaped cells). The hippocampus, septum, and hypothalamus also contain straight radial fibers. In some areas, given their variable orientation, the fibers cannot be regarded as merely persisting radial glia. In the telencephalon, the nuclei basalis, accumbens, ectostriatum, paleostriatum primitivum, and the ventral paleostriatum are particularly rich in GFAP-positive cells, whereas the neostriatum, hyperstriatum, and paleostriatum augmentatum are almost devoid of GFAP labelling. Certain nuclei of the thalamus and the lower brainstem are conspicuous by their low levels of GFAP immunoreactivity. The Bergmann glia were GFAP-immunonegative.
Subst Abus Treatment Prev Pol, 2010
Background and objective: With prolonged use of anabolic androgenic steroids (AAS), occasional in... more Background and objective: With prolonged use of anabolic androgenic steroids (AAS), occasional incidents of renal disorders have been observed. Independently, it has also been established that there are considerable inter-individual and inter-ethnic differences, in particular with reference to the uridine diphosphate-glucuronosyltransferase 2B17 (UGT2B17) gene, in metabolising these compounds. This report postulates the association of deletion polymorphism in the UGT2B17 gene with the occurrence of renal disorders on chronic exposure to AAS. Presentation of the hypothesis: The major deactivation and elimination pathway of AASs is through glucuronide conjugation, chiefly catalyzed by the UGT2B17 enzyme, followed by excretion in urine. Excretion of steroids is affected in individuals with a deletion mutation in the UGT2B17 gene. We hypothesize that UGT2B17 deficient individuals are more vulnerable to developing renal disorders with prolonged use of AAS owing to increases in body mass index and possible direct toxic effects of steroids on the kidneys. Elevated serum levels of biologically active steroids due to inadequate elimination can lead to prolonged muscle build up. An increase in body mass index may cause renal injuries due to sustained elevated glomerular pressure and flow rate. Testing the hypothesis: In the absence of controlled clinical trials in humans, observational studies can be carried out. Real time PCR with allelic discrimination should be employed to examine the prevalence of different UGT2B17 genotypes in patients with impaired renal function and AAS abuse. In individuals with the UGT2B17 deletion polymorphism, blood tests, biofluid analyses, urinalysis, and hair analyses following the administration of an anabolic steroid can be used to determine the fate of the substance once in the body. Implications of the hypothesis: If the hypothesis is upheld, anabolic steroid users with a deletion mutation in the UGT2B17 gene may be exposed to an increased risk of developing renal disorders. In the current detectingsanctioning anti-doping system, athletes motivated by the potential to evade detection owing to their unique genetic make-up could subject themselves to a serious health consequence. More research on AAS metabolism in the presence of UGT2B17 gene deletion is required. Benefit-harm evaluations in therapeutic use of anabolic steroids should also consider this potential link between UGT2B17 gene deletion polymorphism and renal disorders.
Journal of periodontology, 2016
In the present study, the possible localization and role of vascular endothelial growth factor re... more In the present study, the possible localization and role of vascular endothelial growth factor receptor type 2 (VEGFR2) in the regulation of gingival venules in a rat model of experimental diabetes are examined. Six weeks after streptozotocin premedication, Wistar male rats presenting blood sugar levels >20 mmol/L were selected for investigation. The VEGFR2 antagonist ZM323881 [5-((7-benzyloxyquinazolin-4-yl)amino)-4-fluoro-2-methylphenol-hydrochloride] (20 μg/mL) was dripped onto the gingiva between the mandibular incisors. Changes in diameter of the selected gingival venule were measured by vital microscopy combined with digital photography at specified times. Immunohistochemical staining was used to localize VEGFR2. For controls, the same protocol was used on animals with normal blood sugar levels and healthy gingiva. There was a significant difference between the baseline venule diameter of the diabetic and the control groups (47 ± 1 and 28 ± 2 μm, respectively). After 15, 30...
Atlas of the Sensory Organs, 2005
Taste sensation is a form of chemical sense, specialized for the detection of compounds (tastants... more Taste sensation is a form of chemical sense, specialized for the detection of compounds (tastants) dissolved in the saliva. Broadly speaking, taste is just one specific type of visceral sensation that is particularly relevant to food ingestion. Taste signals trigger a host of behavioral and autonomic responses, appetitive or aversive, most of which are visceral reflexes (salivation, gastrointestinal activation, swallowing, gagging, vomiting), or patterns of involuntary locomotor activity (orofacial movements, disgust responses). However, unlike many other visceral signals, taste is also accompanied by conscious perception, recognition, hedonic quality, and memory formation (i.e., predominantly cortical functions). Although these features make taste sensation a truly elaborate faculty, deserving a place among the five principal human senses, the intimate link with visceral sensation is reflected in the remarkably diffuse character of both taste perception and the processing of gustatory (taste-relevant) input.
Journal of Periodontology, 2009
Background: Endothelial cell proliferation, angiogenesis, and increased vascular permeability are... more Background: Endothelial cell proliferation, angiogenesis, and increased vascular permeability are among the effects of vascular endothelial growth factor (VEGF) in various organs. However, the effects of VEGF on gingival hemodynamics, especially on venules, have not been thoroughly investigated. This study investigated the acute circulatory effects of VEGF on rat gingival venules. Methods: Fifty-six anesthetized rats were divided into five study groups; each rat received 10 ml of experimental solution dripped onto the lower interincisal gingiva. The groups included: 1) saline control (after the experiment, gingiva was excised for VEGF receptor 2 [VEGFR2] immunohistochemistry); 2) VEGF (0.1, 1, 10, or 50 mg/ml); 3) VEGF2 receptor antagonist 5-((7-benzyloxyquinazolin-4-yl)amino)-4-fluoro-2-methyl-phenol-hydrochloride (ZM323881; 20 mg/ml); 4) ZM323881 (20 mg/ml) followed by VEGF application (50 mg/ml after 15 minutes); and 5) VEGF (10 mg/ml), these rats were premedicated with nitric oxide (NO) synthase blocker (N G-nitro-L-arginine-methyl-ester [L-NAME]; 1 mg/ ml in drinking water) for 1 week before the experiment. Changes in gingival superficial venule diameter were measured by vital microscopy prior to and 1, 5, 15, 30, and 60 minutes after the administration of the experimental solutions. Results: VEGF dose-dependently increased the venular diameter compared to saline. ZM323881 alone did not cause any alteration. Premedication with ZM323881 or L-NAME decreased the dilatory effects of VEGF. VEGFR2 immunohistochemical labeling was observed in the wall of the venules. Conclusions: There is no remarkable VEGF production under physiologic circumstances in rat gingiva, but VEGF is able to increase gingival blood flow through the activation of VEGF2 receptors. Furthermore, NO release may contribute to VEGF's vasodilatory effect.
Brain Research, 1993
The avian hippocampal formation (HP) is thought to play a role in the processing of spatial memor... more The avian hippocampal formation (HP) is thought to play a role in the processing of spatial memory related to food-storing behaviour. The HP of two food-storing species (marsh tit (Parus palustris) and magpie (Pica pica)) and two non-storing species (great tit (Parus major) and jackdaw (Corvus monedula)) were compared following calbindin-like immunostaining. In the dorsal hippocampal region, both species of food-storing birds had larger calbindin-immunoreactive cells than did the two non-storing species. The fact that this association between storing behaviour and cell morphology is seen in two unrelated families of birds, the Paridae (marsh tit versus great tit) and Corvidae (magpie versus jackdaw) suggests that there may be a direct link between food-storing behaviour and the dorsal hippocampal calbindin-immunoreactive cell population.
Brain structure & function, Jan 6, 2016
Retrograde tracing with choleratoxin B, injected into the nucleus accumbens (Ac) and bed nucleus ... more Retrograde tracing with choleratoxin B, injected into the nucleus accumbens (Ac) and bed nucleus of stria terminalis, lateral part (BSTL), yielded labeled perikarya in a ring-shaped area of arcopallium, including dorsal and hilar subdivisions, with a wedge-shaped node of dense accumulation in the amygdalopiriform area (APir). Also, the position of source neurons for this arcopallio-subpallial pathway was verified by anterograde tracing. Three subregions of arcopallium (amygdalopiriform, dorsal, hilar) were injected with dextran (10 kDa), and fibers and terminal fields were detected in Ac, BSTL and extended amygdala (EA). Most abundant projections to Ac arose from APir. The study enabled precise description of the main output fiber streams: the dorsal stream follows the dorsal border of arcopallium and, continuing in the ventral amygdalofugal tract, it traverses the EA and the BSTL before reaching the Ac. The ventral stream of fibers enters the EA along the ventral subpallial border ...
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Papers by Andrea Dorottya Székely