Proceedings of the International Conference on Maritime and Archipelago (ICoMA 2018), 2019
Sukal, West Bangka district became one of the centers of Anadara sp culture. Not yet known the ef... more Sukal, West Bangka district became one of the centers of Anadara sp culture. Not yet known the effect of shellfish cultivation on the ability of decomposition in mangroves. Cellulolytic bacteria are part of degradation in mangroves. Cellulolytic bacteria have cellulose degradation ability and it made carbohydrates are easier about digested for livestock. This research aims to obtained cellulolytic bacteria in soil, decayed wood and leaf from mangrove with Anadara sp culture in Sukal, West Bangka. The research was conducted from March until June 2018. Isolation was done using agar media with 1% Carboxymethyl Cellulose (CMC). An isolate of bacteria grew in agar with 1% CMC and given the Lugol and Congo Red at 72 hours to test cellulose degradation ability. Isolates from Sukal Mangrove that result the largest cellulose degradation had their species identification using 16S rRNA gene sequenced and microbact analysis. The results of biochemical tests using Mikrobact for bacterial isolates cellulolytic show selected lead to species Bacillus megaterium, Citrobacter freundii, and Vibrio alginoliticus. DNA sequences of isolate bacteria showed close kinship with Pseudomonas psychotolerans.
Biodiversitas Journal of Biological Diversity, 2021
Osteochilus spilurus is a freshwater fish from Southeast Asia. Species identification for this fi... more Osteochilus spilurus is a freshwater fish from Southeast Asia. Species identification for this fish in Indonesia is still solely limited to morphological characteristics. Therefore it is highly important to validate using short DNA sequences that have been proven to be fast, effective, and accurate. Fish specimens were collected from Lenggang river in Belitung island, and Lebak river in Bangka island, Indonesia. Mitochondrial cytochrome b gene (cyt b, 408 bp) was amplified and sequenced. We collected fish specimens from Lenggang River in Belitung Island and Lebak River in Bangka Island, Indonesia. The results suggested that individuals from both islands are clustered together with a strong bootstrap value (100%) in a monophyletic clade. The specimens deposited at NCBI as the first Indonesian genetic records. They have a sister lineage to individuals from Sabah, Malaysia, although supported by a low bootstrap value. While geographical connection during the Pleistocene period allowed wide distribution of the species, differences in the evolutionary processes of historical biogeography and environmental changes are the predicted cause of the low genetic similarity. These findings can be used as a sequence reference and basic information for conservation of this species.
peralatan listrik merupakan sarana yang paling sering digunakan sebagai penunjang untuk memenuhi ... more peralatan listrik merupakan sarana yang paling sering digunakan sebagai penunjang untuk memenuhi kebutuhan sehari-hari. Sebab tanpa adanya listrik maka tidak akan dapat dilakukannya suatu aktifitas di dalam gedung bertingkat yang memang ...
Proceedings of the International Conference on Maritime and Archipelago (ICoMA 2018), 2019
Sukal, West Bangka district became one of the centers of Anadara sp culture. Not yet known the ef... more Sukal, West Bangka district became one of the centers of Anadara sp culture. Not yet known the effect of shellfish cultivation on the ability of decomposition in mangroves. Cellulolytic bacteria are part of degradation in mangroves. Cellulolytic bacteria have cellulose degradation ability and it made carbohydrates are easier about digested for livestock. This research aims to obtained cellulolytic bacteria in soil, decayed wood and leaf from mangrove with Anadara sp culture in Sukal, West Bangka. The research was conducted from March until June 2018. Isolation was done using agar media with 1% Carboxymethyl Cellulose (CMC). An isolate of bacteria grew in agar with 1% CMC and given the Lugol and Congo Red at 72 hours to test cellulose degradation ability. Isolates from Sukal Mangrove that result the largest cellulose degradation had their species identification using 16S rRNA gene sequenced and microbact analysis. The results of biochemical tests using Mikrobact for bacterial isolates cellulolytic show selected lead to species Bacillus megaterium, Citrobacter freundii, and Vibrio alginoliticus. DNA sequences of isolate bacteria showed close kinship with Pseudomonas psychotolerans.
Biodiversitas Journal of Biological Diversity, 2021
Osteochilus spilurus is a freshwater fish from Southeast Asia. Species identification for this fi... more Osteochilus spilurus is a freshwater fish from Southeast Asia. Species identification for this fish in Indonesia is still solely limited to morphological characteristics. Therefore it is highly important to validate using short DNA sequences that have been proven to be fast, effective, and accurate. Fish specimens were collected from Lenggang river in Belitung island, and Lebak river in Bangka island, Indonesia. Mitochondrial cytochrome b gene (cyt b, 408 bp) was amplified and sequenced. We collected fish specimens from Lenggang River in Belitung Island and Lebak River in Bangka Island, Indonesia. The results suggested that individuals from both islands are clustered together with a strong bootstrap value (100%) in a monophyletic clade. The specimens deposited at NCBI as the first Indonesian genetic records. They have a sister lineage to individuals from Sabah, Malaysia, although supported by a low bootstrap value. While geographical connection during the Pleistocene period allowed wide distribution of the species, differences in the evolutionary processes of historical biogeography and environmental changes are the predicted cause of the low genetic similarity. These findings can be used as a sequence reference and basic information for conservation of this species.
peralatan listrik merupakan sarana yang paling sering digunakan sebagai penunjang untuk memenuhi ... more peralatan listrik merupakan sarana yang paling sering digunakan sebagai penunjang untuk memenuhi kebutuhan sehari-hari. Sebab tanpa adanya listrik maka tidak akan dapat dilakukannya suatu aktifitas di dalam gedung bertingkat yang memang ...
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