Jurnal Online Mahasiswa Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau, Feb 1, 2015
Cellulase is an enzyme that can hydrolyze the β-1,4 bonds on cellulose and production of glucose ... more Cellulase is an enzyme that can hydrolyze the β-1,4 bonds on cellulose and production of glucose so that the enzyme is widely used in various industries. Cellulose enzymes can be produced by microorganism such as endophytic bacteria (LBKURCC53, LBKURCC54, and LBKURCC60). The activity of cellulase enzyme was determined by measuring the glucose produced using Nelson-Somogyi method. The optimum activity of cellulase enzyme from the isolates of LBKURCC53, LBKURCC54, and LBKURCC60 we measured at different pH. The enzyme of LBKURCC53 isolate was optimum at pH 7 with enzyme activity of 11,31 ± 1,69 x 10-3 U/mL. While the enzyme of LBKURCC54 and LBKURCC60 isolates were optimum at pH 6,5 with enzyme activity 14,44 ± 2,94 x 10-3 U/mL and 8,33 ± 1,11 x 10-3 U/mL respectively. These comparison of cellulase enzyme activity of isolates LBKURCC53, LBKURCC54, and LBKURCC60 were determined by using Duncan multiple range test at 5% level (α≥0,05).
Jurnal Online Mahasiswa Fakultas Perikanan dan Ilmu Kelautan Universitas Riau, Jun 8, 2015
Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin ... more Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin antimicrobial. Research to analyzed the bacteriocin of 5 probiotic bacterial from isolates tiger shrimp (Penaeus monodon Fabricus). The bacteriocin product contained in 2 forms, that i.e. without precipitation (extract bacteriocin) and with precipitation (fragment bacteriocin) used ammonium sulfate (NH4)2SO4. Bacteriocin will be tested activity against pathogen bacterial that cause fish disease was is Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri. Inhibitory activity test conducted using paper disc diffusion method. Inhibitory activity bacteriocin with precipitation showed inhibition was higher the biggest than without precipitation. Fragment bacteriocin (BC) with precipitation had inhibitory activity or AU (Activity Unit) i.e. 881,71 mm2/ml at UWH9 isolate toward bacteria test A. hydrophila and P. stutzeri. The biggest Inhibitory activity of extract bacteriocin (CR) without precipitation was 365,65mm2/ml at UWH10 isolate toward bacteria test P. stutzeri.
Jurnal Online Mahasiswa Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau, Jun 30, 2014
Dumai water samples are estimated to contain cellulolytic microorganisms because of farm waste. S... more Dumai water samples are estimated to contain cellulolytic microorganisms because of farm waste. Samples 930 from Sei Pakning and 931 from Rupat Strait were water samples containing cellulolytic bacteria taken from Dumai. In this study the production of cellulase enzymes from cellulolytic bacteria in samples of 930 and 931 used CMC as a substrate. Cellulase is an enzyme that catalyses the hidrolisis of β-1-4-glycosidic bond of cellulose. All of the isolates produced cellulase and were able to degrade cellulose. The results indicated that 1% substrate gave the highest activity of Cellulase in the 931-4A (Rupat strait) (1.186 ± 0.319) x 10 -3 U/mL, whereas the highest specific enzyme activity of the bacterial isolates was 930-1C (8.438 ± 0.109) x 10 -3 U/mg.
Jurnal Online Mahasiswa Fakultas Perikanan dan Ilmu Kelautan Universitas Riau, Jun 27, 2015
This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiot... more This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiotic bacteria isolated from giant prawns (Macrobrachium rosenbergii). The method used was experiment method with tested the inhibition against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri). The results showed that bacteriocin capable of inhibiting the growth of pathogenic bacteria of P. stutzeri, A. hydrophila and V. alginolyticus. The best isolates obtained from results of this research was UG4 with clear zones ranging from 7.2 to 11.4 mm and the inhibition activity of 1476.53 mm²/mL after fragmentation with (NH 4) 2 SO 4 against pathogenic bacteria P. stutzeri was incubated at room temperature (37°C). This inhibition was caused by the presence of antimicrobial compounds that are produced by probiotic bacteria, namely bacteriocin. For isolates UG2 codes, the bacteria was resistant in which characterized by the formation of bluring zone around the paper disc.
This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiot... more This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiotic bacteria isolated from giant prawns (Macrobrachium rosenbergii). The method used was experiment method with tested the inhibition against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri). The results showed that bacteriocin capable of inhibiting the growth of pathogenic bacteria of P. stutzeri, A. hydrophila and V. alginolyticus. The best isolates obtained from results of this research was UG4 with clear zones ranging from 7.2 to 11.4 mm and the inhibition activity of 1476.53 mm²/mL after fragmentation with (NH4)2SO4 against pathogenic bacteria P. stutzeri was incubated at room temperature (37°C). This inhibition was caused by the presence of antimicrobial compounds that are produced by probiotic bacteria, namely bacteriocin. For isolates UG2 codes, the bacteria was resistant in which characterized by the formation of bluring zone around the pa...
Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin ... more Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin antimicrobial. Research to analyzed the bacteriocin of 5 probiotic bacterial from isolates tiger shrimp (Penaeus monodon Fabricus). The bacteriocin product contained in 2 forms, that i.e. without precipitation (extract bacteriocin) and with precipitation (fragment bacteriocin) used ammonium sulfate (NH4)2SO4. Bacteriocin will be tested activity against pathogen bacterial that cause fish disease was is Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri. Inhibitory activity test conducted using paper disc diffusion method. Inhibitory activity bacteriocin with precipitation showed inhibition was higher the biggest than without precipitation. Fragment bacteriocin (BC) with precipitation had inhibitory activity or AU (Activity Unit) i.e. 881,71 mm2/ml at UWH9 isolate toward bacteria test A. hydrophila and P. stutzeri. The biggest Inhibitory activity of extract bacteriocin (CR...
Kepok banana (Musa paradisiaca) peel waste contain sugar that can be changed to acetic aci... more Kepok banana (Musa paradisiaca) peel waste contain sugar that can be changed to acetic acid using Effective Microorganism (EM-4), kombucha (KO), cassava yeast (RT) and instant yeast (RI) (commercial starter). The Commercial starter contains variety of bacteria and yeasts with different content of microorganism that will influence the amount of ethanol produced. The aim of this study to determine the potential of kepok banana peel waste used as the main material in synthesis of acetic acid using commercial starter. Based on the fermentation results using the four starter, cassava yeast has a good ability to be fermented. The acetic acid formation was obtained under condition which substrate and starter amount are 80% w/v and 5 grams respectively and fermentation time 4 day. Based on these conditions it was found that the amount of organic acid total determined by titrimetric method is 4.712±0.066% and acetic acid formed which was ...
IOP Conference Series: Earth and Environmental Science, 2020
Fluorene is one of the Polycyclic Aromatic Hydrocarbons (PAHs) that has toxic, mutagenic, and giv... more Fluorene is one of the Polycyclic Aromatic Hydrocarbons (PAHs) that has toxic, mutagenic, and gives a persistent effect in the environment. One of the alternative ways that can be done to overcome environmental problems due to contamination of fluorene is biodegradation using marine bacteria. There are 25 marine bacterial isolates from Laboratory of Biocatalyst and Fermentation (LBF), Research Center for Biotechnology LIPI Cibinong, Bogor which have capability to degrade fluorene. Selection of these 25 marine bacterial isolates showed that LBF-1-0144 isolate was the good relatively in degrading fluorene. This isolate was able to grow in 1500 ppm fluorene containing medium with an initial cell OD600nm of 2.5 and growth percentage of 58.652%. Molecular identification results based on the 16S rDNA gene of the LBF-1-0144 showed that this isolate had similarity of 96% with Bacillus subtilis strain 168.
Jurnal Online Mahasiswa Bidang Matematika Dan Ilmu Pengetahuan Alam, Jan 14, 2015
Bacterial cellulose is a potential biopolymer that can be used in the medical field as an antibac... more Bacterial cellulose is a potential biopolymer that can be used in the medical field as an antibacterial membrane. Antibacterial membrane was synthesized from bacterial cellulose that was composited with silver nanoparticle that has antibacterial activity. Silver nanoparticles were impregnated into bacterial cellulose membrane to produce bacterial cellulose-Ag bionanocomposite membrane. Synthesis of silver nanoparticles was performed using reduction method with various concentrations of AgNO 3 as precursor (0.5 mM, 1 mM, 1.5 mM) and sodium citrate as reducing and stabilizing agent. Bacterial cellulose-Ag bionanocomposite membrane was characterized by Scanning Electron Microscopy-Energy Dispersive X-Ray Spectroscopy (SEM-EDX). The results of SEM-EDX characterization showed that bacterial cellulose-Ag bionanocomposite membrane with AgNO 3 0.5 mM has smallest size of silver nanoparticles (30-60 nm) and distributed homogeneously in the bacterial cellulose membrane. Antibacterial test against Staphylococcus aureus and Escherichia coli was carried out by the agar diffusion and turbidimetry method. Bacterial cellulose-Ag bionanocomposite membrane did not show antibacterial activity in the agar diffusion method. In turbidimetry method, bacterial cellulose-Ag bionanocomposite membrane with AgNO 3 0.5 mM showed antibacterial activity against Staphylococcus aureus and Escherichia coli. It was able to decrease Optical Density of Staphylococcus aureus (26,69%) and Escherichia coli (22,91%) compared to controls.
Jurnal Online Mahasiswa Bidang Matematika Dan Ilmu Pengetahuan Alam, May 29, 2014
Kepok banana (Musa paradisiaca) peel waste contain sugar that can be changed to acetic acid using... more Kepok banana (Musa paradisiaca) peel waste contain sugar that can be changed to acetic acid using Effective Microorganism (EM-4), kombucha (KO), cassava yeast (RT) and instant yeast (RI) (commercial starter). The Commercial starter contains variety of bacteria and yeasts with different content of microorganism that will influence the amount of ethanol produced. The aim of this study to determine the potential of kepok banana peel waste used as the main material in synthesis of acetic acid using commercial starter. Based on the fermentation results using the four starter, cassava yeast has a good ability to be fermented. The acetic acid formation was obtained under condition which substrate and starter amount are 80% w/v and 5 grams respectively and fermentation time 4 day. Based on these conditions it was found that the amount of organic acid total determined by titrimetric method is 4.712±0.066% and acetic acid formed which was determined by Ion Chromatography method is 0.7507± 0.0538%.
Jurnal Online Mahasiswa Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau, Feb 1, 2015
Cellulase is an enzyme that can hydrolyze the β-1,4 bonds on cellulose and production of glucose ... more Cellulase is an enzyme that can hydrolyze the β-1,4 bonds on cellulose and production of glucose so that the enzyme is widely used in various industries. Cellulose enzymes can be produced by microorganism such as endophytic bacteria (LBKURCC53, LBKURCC54, and LBKURCC60). The activity of cellulase enzyme was determined by measuring the glucose produced using Nelson-Somogyi method. The optimum activity of cellulase enzyme from the isolates of LBKURCC53, LBKURCC54, and LBKURCC60 we measured at different pH. The enzyme of LBKURCC53 isolate was optimum at pH 7 with enzyme activity of 11,31 ± 1,69 x 10-3 U/mL. While the enzyme of LBKURCC54 and LBKURCC60 isolates were optimum at pH 6,5 with enzyme activity 14,44 ± 2,94 x 10-3 U/mL and 8,33 ± 1,11 x 10-3 U/mL respectively. These comparison of cellulase enzyme activity of isolates LBKURCC53, LBKURCC54, and LBKURCC60 were determined by using Duncan multiple range test at 5% level (α≥0,05).
Jurnal Online Mahasiswa Fakultas Perikanan dan Ilmu Kelautan Universitas Riau, Jun 8, 2015
Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin ... more Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin antimicrobial. Research to analyzed the bacteriocin of 5 probiotic bacterial from isolates tiger shrimp (Penaeus monodon Fabricus). The bacteriocin product contained in 2 forms, that i.e. without precipitation (extract bacteriocin) and with precipitation (fragment bacteriocin) used ammonium sulfate (NH4)2SO4. Bacteriocin will be tested activity against pathogen bacterial that cause fish disease was is Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri. Inhibitory activity test conducted using paper disc diffusion method. Inhibitory activity bacteriocin with precipitation showed inhibition was higher the biggest than without precipitation. Fragment bacteriocin (BC) with precipitation had inhibitory activity or AU (Activity Unit) i.e. 881,71 mm2/ml at UWH9 isolate toward bacteria test A. hydrophila and P. stutzeri. The biggest Inhibitory activity of extract bacteriocin (CR) without precipitation was 365,65mm2/ml at UWH10 isolate toward bacteria test P. stutzeri.
Jurnal Online Mahasiswa Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau, Jun 30, 2014
Dumai water samples are estimated to contain cellulolytic microorganisms because of farm waste. S... more Dumai water samples are estimated to contain cellulolytic microorganisms because of farm waste. Samples 930 from Sei Pakning and 931 from Rupat Strait were water samples containing cellulolytic bacteria taken from Dumai. In this study the production of cellulase enzymes from cellulolytic bacteria in samples of 930 and 931 used CMC as a substrate. Cellulase is an enzyme that catalyses the hidrolisis of β-1-4-glycosidic bond of cellulose. All of the isolates produced cellulase and were able to degrade cellulose. The results indicated that 1% substrate gave the highest activity of Cellulase in the 931-4A (Rupat strait) (1.186 ± 0.319) x 10 -3 U/mL, whereas the highest specific enzyme activity of the bacterial isolates was 930-1C (8.438 ± 0.109) x 10 -3 U/mg.
Jurnal Online Mahasiswa Fakultas Perikanan dan Ilmu Kelautan Universitas Riau, Jun 27, 2015
This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiot... more This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiotic bacteria isolated from giant prawns (Macrobrachium rosenbergii). The method used was experiment method with tested the inhibition against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri). The results showed that bacteriocin capable of inhibiting the growth of pathogenic bacteria of P. stutzeri, A. hydrophila and V. alginolyticus. The best isolates obtained from results of this research was UG4 with clear zones ranging from 7.2 to 11.4 mm and the inhibition activity of 1476.53 mm²/mL after fragmentation with (NH 4) 2 SO 4 against pathogenic bacteria P. stutzeri was incubated at room temperature (37°C). This inhibition was caused by the presence of antimicrobial compounds that are produced by probiotic bacteria, namely bacteriocin. For isolates UG2 codes, the bacteria was resistant in which characterized by the formation of bluring zone around the paper disc.
This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiot... more This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiotic bacteria isolated from giant prawns (Macrobrachium rosenbergii). The method used was experiment method with tested the inhibition against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri). The results showed that bacteriocin capable of inhibiting the growth of pathogenic bacteria of P. stutzeri, A. hydrophila and V. alginolyticus. The best isolates obtained from results of this research was UG4 with clear zones ranging from 7.2 to 11.4 mm and the inhibition activity of 1476.53 mm²/mL after fragmentation with (NH4)2SO4 against pathogenic bacteria P. stutzeri was incubated at room temperature (37°C). This inhibition was caused by the presence of antimicrobial compounds that are produced by probiotic bacteria, namely bacteriocin. For isolates UG2 codes, the bacteria was resistant in which characterized by the formation of bluring zone around the pa...
Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin ... more Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin antimicrobial. Research to analyzed the bacteriocin of 5 probiotic bacterial from isolates tiger shrimp (Penaeus monodon Fabricus). The bacteriocin product contained in 2 forms, that i.e. without precipitation (extract bacteriocin) and with precipitation (fragment bacteriocin) used ammonium sulfate (NH4)2SO4. Bacteriocin will be tested activity against pathogen bacterial that cause fish disease was is Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri. Inhibitory activity test conducted using paper disc diffusion method. Inhibitory activity bacteriocin with precipitation showed inhibition was higher the biggest than without precipitation. Fragment bacteriocin (BC) with precipitation had inhibitory activity or AU (Activity Unit) i.e. 881,71 mm2/ml at UWH9 isolate toward bacteria test A. hydrophila and P. stutzeri. The biggest Inhibitory activity of extract bacteriocin (CR...
Kepok banana (Musa paradisiaca) peel waste contain sugar that can be changed to acetic aci... more Kepok banana (Musa paradisiaca) peel waste contain sugar that can be changed to acetic acid using Effective Microorganism (EM-4), kombucha (KO), cassava yeast (RT) and instant yeast (RI) (commercial starter). The Commercial starter contains variety of bacteria and yeasts with different content of microorganism that will influence the amount of ethanol produced. The aim of this study to determine the potential of kepok banana peel waste used as the main material in synthesis of acetic acid using commercial starter. Based on the fermentation results using the four starter, cassava yeast has a good ability to be fermented. The acetic acid formation was obtained under condition which substrate and starter amount are 80% w/v and 5 grams respectively and fermentation time 4 day. Based on these conditions it was found that the amount of organic acid total determined by titrimetric method is 4.712±0.066% and acetic acid formed which was ...
IOP Conference Series: Earth and Environmental Science, 2020
Fluorene is one of the Polycyclic Aromatic Hydrocarbons (PAHs) that has toxic, mutagenic, and giv... more Fluorene is one of the Polycyclic Aromatic Hydrocarbons (PAHs) that has toxic, mutagenic, and gives a persistent effect in the environment. One of the alternative ways that can be done to overcome environmental problems due to contamination of fluorene is biodegradation using marine bacteria. There are 25 marine bacterial isolates from Laboratory of Biocatalyst and Fermentation (LBF), Research Center for Biotechnology LIPI Cibinong, Bogor which have capability to degrade fluorene. Selection of these 25 marine bacterial isolates showed that LBF-1-0144 isolate was the good relatively in degrading fluorene. This isolate was able to grow in 1500 ppm fluorene containing medium with an initial cell OD600nm of 2.5 and growth percentage of 58.652%. Molecular identification results based on the 16S rDNA gene of the LBF-1-0144 showed that this isolate had similarity of 96% with Bacillus subtilis strain 168.
Jurnal Online Mahasiswa Bidang Matematika Dan Ilmu Pengetahuan Alam, Jan 14, 2015
Bacterial cellulose is a potential biopolymer that can be used in the medical field as an antibac... more Bacterial cellulose is a potential biopolymer that can be used in the medical field as an antibacterial membrane. Antibacterial membrane was synthesized from bacterial cellulose that was composited with silver nanoparticle that has antibacterial activity. Silver nanoparticles were impregnated into bacterial cellulose membrane to produce bacterial cellulose-Ag bionanocomposite membrane. Synthesis of silver nanoparticles was performed using reduction method with various concentrations of AgNO 3 as precursor (0.5 mM, 1 mM, 1.5 mM) and sodium citrate as reducing and stabilizing agent. Bacterial cellulose-Ag bionanocomposite membrane was characterized by Scanning Electron Microscopy-Energy Dispersive X-Ray Spectroscopy (SEM-EDX). The results of SEM-EDX characterization showed that bacterial cellulose-Ag bionanocomposite membrane with AgNO 3 0.5 mM has smallest size of silver nanoparticles (30-60 nm) and distributed homogeneously in the bacterial cellulose membrane. Antibacterial test against Staphylococcus aureus and Escherichia coli was carried out by the agar diffusion and turbidimetry method. Bacterial cellulose-Ag bionanocomposite membrane did not show antibacterial activity in the agar diffusion method. In turbidimetry method, bacterial cellulose-Ag bionanocomposite membrane with AgNO 3 0.5 mM showed antibacterial activity against Staphylococcus aureus and Escherichia coli. It was able to decrease Optical Density of Staphylococcus aureus (26,69%) and Escherichia coli (22,91%) compared to controls.
Jurnal Online Mahasiswa Bidang Matematika Dan Ilmu Pengetahuan Alam, May 29, 2014
Kepok banana (Musa paradisiaca) peel waste contain sugar that can be changed to acetic acid using... more Kepok banana (Musa paradisiaca) peel waste contain sugar that can be changed to acetic acid using Effective Microorganism (EM-4), kombucha (KO), cassava yeast (RT) and instant yeast (RI) (commercial starter). The Commercial starter contains variety of bacteria and yeasts with different content of microorganism that will influence the amount of ethanol produced. The aim of this study to determine the potential of kepok banana peel waste used as the main material in synthesis of acetic acid using commercial starter. Based on the fermentation results using the four starter, cassava yeast has a good ability to be fermented. The acetic acid formation was obtained under condition which substrate and starter amount are 80% w/v and 5 grams respectively and fermentation time 4 day. Based on these conditions it was found that the amount of organic acid total determined by titrimetric method is 4.712±0.066% and acetic acid formed which was determined by Ion Chromatography method is 0.7507± 0.0538%.
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