In this work a composite indicator is built, named the Colombia�s Children Deprivation Index (CCD... more In this work a composite indicator is built, named the Colombia�s Children Deprivation Index (CCDI). The CCDI summarizes the access to a set of goods and services in key areas for welfare and compliance with children�s rights. In particular, the living conditions measured by the CCDI indicate whether children: a) are legally detectable by the government, if their mothers enjoy a minimum level of education and pre-natal care that contributes to their welfare, if they are protected against health risks, if they attend school, if they live in decent housing, if they have acceptable levels of nutrition and if they are not prematurely exposed to adult-related obligations such as maternity and work. The best score, or ideal situation of the CCDI is zero (0), which shows that no child is exposed to the limitations signified by the selected partial indicators, and in the worst cases it is one (1). The results show that the minimum advances in children�s living conditions are concentrated in...
• Resumen: En este documento identificamos un conjunto de indicadores a nivel departamental y mun... more • Resumen: En este documento identificamos un conjunto de indicadores a nivel departamental y municipal (en la medida en que la información lo permite) con perspectiva de niñez, indicadores que facilitan analizar el bienestar y cumplimiento de los derechos de los niños y niñas en distintas áreas: salud, educación, alimentación, vivienda digna, y la exposición a riesgos tempranos como el trabajo y la maternidad. La construcción de indicadores a nivel municipal que reflejan el bienestar y/o el cumplimiento de los derechos, ofrece una oportunidad para obtener una imagen completa de la situación de la niñez en un departamento, y constituye una herramienta sumamente importante en el diseño de la política pública y la focalización del gasto. Los indicadores del Valle del Cauca aquí presentados reflejan inequidades geográficas en la provisión y acceso de servicios sociales básicos; en ellos se evidencian grandes brechas entre municipios frente al promedio departamental y la ciudad de Cali. Los mayores rezagos se concentran en algunos municipios del norte del Valle y el municipio de Buenaventura. Todo esto plantea importantes retos de política pública, e invitan al debate, al análisis y a la visualización de las condiciones de vida de la niñez en el Valle del Cauca.
· Resumen: En el presente artículo analizamos determinantes (individuales y de contexto) que inci... more · Resumen: En el presente artículo analizamos determinantes (individuales y de contexto) que inciden en el uso de servicios de atención en salud por parte de las madres, antes, durante y después del parto, en el Litoral Pacífico Colombiano. Para este objetivo construimos indicadores estadísticos y estimamos modelos de regresión binaria a partir de microdatos indicadores calculados y los modelos estimados muestran que las variables que más inciden en el uso -por parte de las madres-de los servicios de atención prenatal calificada, parto institucional y atención posparto son, en orden de importancia: la afiliación a un régimen de seguridad social en salud, el orden de nacimiento de los hijos e hijas, el nivel de educación y el lugar de residencia de la madre. De otra parte, se hace visible en este trabajo la profunda inequidad territorial que exhibe el litoral pacífico colombiano, cuando se analiza en forma específica su situación frente al promedio nacional en cuanto a la provisión y acceso a servicios sociales básicos.
We compared the performance of two clinical groups, Williams syndrome (WS) and Smith-Magenis synd... more We compared the performance of two clinical groups, Williams syndrome (WS) and Smith-Magenis syndrome (SMS), in terms of concrete and relational vocabulary. We analyzed (a) whether the WS group had an advantage in concrete vocabulary when compared to the SMS group, as good concrete vocabulary knowledge is considered a hallmark of WS; (b) if spatial processing difficulties in WS would be reflected specifically in their knowledge of relational spatial vocabulary; (c) if a specific vocabulary profile could be outlined for SMS. Our results show similar performances on receptive concrete and relational vocabulary in both groups. However, and as anticipated, performance on relational space concepts was significantly lower in the WS group.
International Journal of Developmental Disabilities, 2013
ABSTRACT Objectives: This study examined data on multidimensional functioning in a group of indiv... more ABSTRACT Objectives: This study examined data on multidimensional functioning in a group of individuals with Smith–Magenis syndrome (SMS). Methods: Parents of 19 children, adolescents, and young adults with SMS were interviewed on developmental, physical, medical, behavioral, and social-communicative aspects. Results: The data presented here provided relevant neurodevelopmental information, and focused not only on problems and deficits, but also on areas of relative strength, particularly social. Discussion: Our study contributed to further the understanding on the prevalence of a broad set of characteristics in a sample of individuals with SMS from a wide age range.
... Barco and Guadalupe formations. Mirador formation is being also affected by high GOR currentl... more ... Barco and Guadalupe formations. Mirador formation is being also affected by high GOR currently, being observed in most of Mirador wells, however in this study was not possible to discriminate that effect, due to limitations of current IPR models. Page 6. 6 A. PRADA., GE ...
This paper examined the association between individual and local level factors and the number of ... more This paper examined the association between individual and local level factors and the number of antenatal care visits completed by women in Colombia using data from the 2010 Colombian Demographic and Health Survey and multilevel logistic regression models. Our findings suggest that, in addition to maternal socioeconomic status, contextual factors influence whether pregnant women complete the minimum recommended number of antenatal care visits. These factors include: level of women's autonomy in the community, regional inequalities and access barriers caused by distance (OR = 0.057), costs of services (OR = 0.035), and/or a lack of confidence in doctors (OR = 0.036). Our results highlight the existence of inequalities in access to antenatal care and the importance of considering the local context in the design of effective maternal care policies in Colombia. Furthermore, our findings regarding individual factors corroborate the evidence from other countries and offer new insights into the association between local level factors and number of antenatal care visits.
ABSTRACT Contextual effects on child health have been investigated extensively in previous resear... more ABSTRACT Contextual effects on child health have been investigated extensively in previous research. However, few studies have considered the interplay between community characteristics and individual-level variables. This study examines the influence of community education and family socioeconomic characteristics on child health (as measured by height and weight-for-age Z-scores), as well as their interactions. We adapted the Commission on Social Determinants of Health (CSDH) framework to the context of child health. Using data from the 2010 Colombian Demographic and Health Survey (DHS), weighted multilevel models are fitted since the data are not self-weighting. The results show a positive impact of the level of education of other women in the community on child health, even after controlling for individual and family socioeconomic characteristics. Different pathways through which community education can substitute for the effect of family characteristics on child nutrition are found. The interaction terms highlight the importance of community education as a moderator of the impact of the mother’s own education and autonomy, on child health. In addition, the results reveal differences between height and weight-for-age indicators in their responsiveness to individual and contextual factors. Our findings suggest that community intervention programmes may have differential effects on child health. Therefore, their identification can contribute to a better targeting of child care policies.
A theoretical study of a diluted and random-bond Ising model applied to the magnetic properties o... more A theoretical study of a diluted and random-bond Ising model applied to the magnetic properties of the fcc disordered phase of Fe-Mn-Al alloys is reported. Using the variational Bogoliubov inequality approach, it was possible to obtain mean hyperfine field data at room temperature as a function of concentration and these are in agreement with the experimental data previously reported by Pérez Alcázar, Galvão da Silva, and Paduani ͓Hyperfine Int. 66, 221 ͑1991͔͒.
This study examines how structural determinants influence intermediary factors of child health in... more This study examines how structural determinants influence intermediary factors of child health inequities and how they operate through the communities where children live. In particular, we explore individual, family and community level characteristics associated with a composite indicator that quantitatively measures intermediary determinants of early childhood health in Colombia. We use data from the 2010 Colombian Demographic and Health Survey (DHS). Adopting the conceptual framework of the Commission on Social Determinants of Health (CSDH), three dimensions related to child health are represented in the index: behavioural factors, psychosocial factors and health system. In order to generate the weight of the variables and take into account the discrete nature of the data, principal component analysis (PCA) using polychoric correlations are employed in the index construction. Weighted multilevel models are used to examine community effects. The results show that the effect of household's SES is attenuated when community characteristics are included, indicating the importance that the level of community development may have in mediating individual and family characteristics. The findings indicate that there is a significant variance in intermediary determinants of child health between-community, especially for those determinants linked to the health system, even after controlling for individual, family and community characteristics. These results likely reflect that whilst the community context can exert a greater influence on intermediary factors linked directly to health, in the case of psychosocial factors and the parent's behaviours, the family context can be more important. This underlines the importance of distinguishing between community and family intervention programmes.
Post-mortem bacterial culture and specific biochemical tests are currently performed to character... more Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. Regarding the analytical sensitivity, DNA of the M. bovis AN5 strain was detected up to 1.5 pg by nested-PCR, whereas DNA of M. tuberculosis H37Rv strain was detected up to 6.1 pg. The nested-PCR system showed 100% analytical specificity for MTC when tested with DNA of reference strains of non-tuberculous mycobacteria and closely-related Actinomycetales. A clinical sensitivity level of 76.7% was detected with tissues samples positive for MTC by means of the culture and conventional PCR. A clinical specificity of 100% was detected with DNA from tissue samples of cattle with negative results in the comparative intradermal tuberculin test. These cattle exhibited no visible lesions and were negative in the culture for MTC. The use of the nested-PCR assay to detect M. tuberculosis complex in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. Table 1 -Bacterial strains used to assess the analytical specificity or sensitivity of the nested-PCR. Bacterial strains Strain /origin Corynebacterium pseudotuberculosis LGCM/FIOCRUZ* Mycobacterium abscessus ATCC 19977/FIOCRUZ Mycobacterium avium ATCC 25291/FIOCRUZ Mycobacterium bovis AN5 strain, Ministry of Agriculture -LANAGRO-MG Mycobacterium fortuitum ATCC 6841/FIOCRUZ Mycobacterium gordonae ATCC 14470/FIOCRUZ Mycobacterium kansasii ATCC 12478/FIOCRUZ Mycobacterium tuberculosis H37Rv/FIOCRUZ Mycobacterium smegmatis ATCC 700044/FIOCRUZ Rhodococcus equi ATCC 6939/FIOCRUZ
En este artículo se estima una línea de pobreza subjetiva (LPS), a través de las respuestas de lo... more En este artículo se estima una línea de pobreza subjetiva (LPS), a través de las respuestas de los hogares colombianos y vallecaucanos a la pregunta de suficiencia de ingresos de la Encuesta de Calidad de Vida 2003 (ECV2003). Los resultados encontrados muestran que un hogar en Colombia se considera pobre si sus ingresos en pesos colombianos son menores a $1.142.097,
The parasitic infection neurocysticercosis may give rise to a variety of psychiatric manifestatio... more The parasitic infection neurocysticercosis may give rise to a variety of psychiatric manifestations that resemble, but are different from, primary psychiatric disorders. The aim of this study was to determine if among individuals from a neurocysticercosis-endemic area of Colombia who apparently had a psychiatric manifestation with associated neurological finding ('cases'), some could have been infected with Taenia solium cysticerci. This case-control study was done in individuals hospitalized in two mental institutions. The control-1 individuals were those classified with primary psychiatric disease, and the control-2 group consisted of healthy, non-hospitalized individuals. A serological test for cysticercosis was positive in 5/96 (5.1%) cases, 4/153 (2.6%) psychiatric controls, and 5/246 (2%) healthy controls. The data analysis indicated a weak association between the cases and a positive serology for neurocysticercosis (odds ratio > 2; P > 0.05). The lower education level of the cases influenced this association.
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance o... more In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT-results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT-animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. Citation: Araú jo CP, Osó rio ALAR, Jorge KSG, Ramos CAN, Filho AFS, et al. (2014) Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. PLoS ONE 9(3): e91023.
The thermodynamic properties of a ternary magnetic system are studied by considering a random-sit... more The thermodynamic properties of a ternary magnetic system are studied by considering a random-site Ising model in the context of the Bogoliubov inequality for the free energy. Only nearest-neighbor sites are considered in order to account for the different interactions in the model. The critical temperature, magnetization, and other quantities have been obtained in an approximation which reproduces the exact results at least in one dimension. The calculations are used to interpret the experimental data of the mean hyperfine field obtained by Mössbauer spectroscopy and the critical temperature obtained by calorimetry measurements of the antiferromagnetic ternary Fe-Mn-Al alloys in the fcc-disordered phase.
Pesq. Vet. Bras. 18(2): 75-82, abr./jun. 1998 75 RESUMO.-Quinze focos de meningoencefalite por he... more Pesq. Vet. Bras. 18(2): 75-82, abr./jun. 1998 75 RESUMO.-Quinze focos de meningoencefalite por herpesvírus bovino-5 (BHV-5) foram diagnosticados entre agosto de 1993 e dezembro de 1996, sendo 14 provenientes do estado do Mato Grosso do Sul e um do estado de São Paulo. A doença ocorreu em diversos municípios e em diferentes épocas do ano. Foram afetados bovinos de 6 a 60 meses de idade, com uma morbidade de 0,05% a 5% e letalidade próxima a 100%.
In this work a composite indicator is built, named the Colombia�s Children Deprivation Index (CCD... more In this work a composite indicator is built, named the Colombia�s Children Deprivation Index (CCDI). The CCDI summarizes the access to a set of goods and services in key areas for welfare and compliance with children�s rights. In particular, the living conditions measured by the CCDI indicate whether children: a) are legally detectable by the government, if their mothers enjoy a minimum level of education and pre-natal care that contributes to their welfare, if they are protected against health risks, if they attend school, if they live in decent housing, if they have acceptable levels of nutrition and if they are not prematurely exposed to adult-related obligations such as maternity and work. The best score, or ideal situation of the CCDI is zero (0), which shows that no child is exposed to the limitations signified by the selected partial indicators, and in the worst cases it is one (1). The results show that the minimum advances in children�s living conditions are concentrated in...
• Resumen: En este documento identificamos un conjunto de indicadores a nivel departamental y mun... more • Resumen: En este documento identificamos un conjunto de indicadores a nivel departamental y municipal (en la medida en que la información lo permite) con perspectiva de niñez, indicadores que facilitan analizar el bienestar y cumplimiento de los derechos de los niños y niñas en distintas áreas: salud, educación, alimentación, vivienda digna, y la exposición a riesgos tempranos como el trabajo y la maternidad. La construcción de indicadores a nivel municipal que reflejan el bienestar y/o el cumplimiento de los derechos, ofrece una oportunidad para obtener una imagen completa de la situación de la niñez en un departamento, y constituye una herramienta sumamente importante en el diseño de la política pública y la focalización del gasto. Los indicadores del Valle del Cauca aquí presentados reflejan inequidades geográficas en la provisión y acceso de servicios sociales básicos; en ellos se evidencian grandes brechas entre municipios frente al promedio departamental y la ciudad de Cali. Los mayores rezagos se concentran en algunos municipios del norte del Valle y el municipio de Buenaventura. Todo esto plantea importantes retos de política pública, e invitan al debate, al análisis y a la visualización de las condiciones de vida de la niñez en el Valle del Cauca.
· Resumen: En el presente artículo analizamos determinantes (individuales y de contexto) que inci... more · Resumen: En el presente artículo analizamos determinantes (individuales y de contexto) que inciden en el uso de servicios de atención en salud por parte de las madres, antes, durante y después del parto, en el Litoral Pacífico Colombiano. Para este objetivo construimos indicadores estadísticos y estimamos modelos de regresión binaria a partir de microdatos indicadores calculados y los modelos estimados muestran que las variables que más inciden en el uso -por parte de las madres-de los servicios de atención prenatal calificada, parto institucional y atención posparto son, en orden de importancia: la afiliación a un régimen de seguridad social en salud, el orden de nacimiento de los hijos e hijas, el nivel de educación y el lugar de residencia de la madre. De otra parte, se hace visible en este trabajo la profunda inequidad territorial que exhibe el litoral pacífico colombiano, cuando se analiza en forma específica su situación frente al promedio nacional en cuanto a la provisión y acceso a servicios sociales básicos.
We compared the performance of two clinical groups, Williams syndrome (WS) and Smith-Magenis synd... more We compared the performance of two clinical groups, Williams syndrome (WS) and Smith-Magenis syndrome (SMS), in terms of concrete and relational vocabulary. We analyzed (a) whether the WS group had an advantage in concrete vocabulary when compared to the SMS group, as good concrete vocabulary knowledge is considered a hallmark of WS; (b) if spatial processing difficulties in WS would be reflected specifically in their knowledge of relational spatial vocabulary; (c) if a specific vocabulary profile could be outlined for SMS. Our results show similar performances on receptive concrete and relational vocabulary in both groups. However, and as anticipated, performance on relational space concepts was significantly lower in the WS group.
International Journal of Developmental Disabilities, 2013
ABSTRACT Objectives: This study examined data on multidimensional functioning in a group of indiv... more ABSTRACT Objectives: This study examined data on multidimensional functioning in a group of individuals with Smith–Magenis syndrome (SMS). Methods: Parents of 19 children, adolescents, and young adults with SMS were interviewed on developmental, physical, medical, behavioral, and social-communicative aspects. Results: The data presented here provided relevant neurodevelopmental information, and focused not only on problems and deficits, but also on areas of relative strength, particularly social. Discussion: Our study contributed to further the understanding on the prevalence of a broad set of characteristics in a sample of individuals with SMS from a wide age range.
... Barco and Guadalupe formations. Mirador formation is being also affected by high GOR currentl... more ... Barco and Guadalupe formations. Mirador formation is being also affected by high GOR currently, being observed in most of Mirador wells, however in this study was not possible to discriminate that effect, due to limitations of current IPR models. Page 6. 6 A. PRADA., GE ...
This paper examined the association between individual and local level factors and the number of ... more This paper examined the association between individual and local level factors and the number of antenatal care visits completed by women in Colombia using data from the 2010 Colombian Demographic and Health Survey and multilevel logistic regression models. Our findings suggest that, in addition to maternal socioeconomic status, contextual factors influence whether pregnant women complete the minimum recommended number of antenatal care visits. These factors include: level of women's autonomy in the community, regional inequalities and access barriers caused by distance (OR = 0.057), costs of services (OR = 0.035), and/or a lack of confidence in doctors (OR = 0.036). Our results highlight the existence of inequalities in access to antenatal care and the importance of considering the local context in the design of effective maternal care policies in Colombia. Furthermore, our findings regarding individual factors corroborate the evidence from other countries and offer new insights into the association between local level factors and number of antenatal care visits.
ABSTRACT Contextual effects on child health have been investigated extensively in previous resear... more ABSTRACT Contextual effects on child health have been investigated extensively in previous research. However, few studies have considered the interplay between community characteristics and individual-level variables. This study examines the influence of community education and family socioeconomic characteristics on child health (as measured by height and weight-for-age Z-scores), as well as their interactions. We adapted the Commission on Social Determinants of Health (CSDH) framework to the context of child health. Using data from the 2010 Colombian Demographic and Health Survey (DHS), weighted multilevel models are fitted since the data are not self-weighting. The results show a positive impact of the level of education of other women in the community on child health, even after controlling for individual and family socioeconomic characteristics. Different pathways through which community education can substitute for the effect of family characteristics on child nutrition are found. The interaction terms highlight the importance of community education as a moderator of the impact of the mother’s own education and autonomy, on child health. In addition, the results reveal differences between height and weight-for-age indicators in their responsiveness to individual and contextual factors. Our findings suggest that community intervention programmes may have differential effects on child health. Therefore, their identification can contribute to a better targeting of child care policies.
A theoretical study of a diluted and random-bond Ising model applied to the magnetic properties o... more A theoretical study of a diluted and random-bond Ising model applied to the magnetic properties of the fcc disordered phase of Fe-Mn-Al alloys is reported. Using the variational Bogoliubov inequality approach, it was possible to obtain mean hyperfine field data at room temperature as a function of concentration and these are in agreement with the experimental data previously reported by Pérez Alcázar, Galvão da Silva, and Paduani ͓Hyperfine Int. 66, 221 ͑1991͔͒.
This study examines how structural determinants influence intermediary factors of child health in... more This study examines how structural determinants influence intermediary factors of child health inequities and how they operate through the communities where children live. In particular, we explore individual, family and community level characteristics associated with a composite indicator that quantitatively measures intermediary determinants of early childhood health in Colombia. We use data from the 2010 Colombian Demographic and Health Survey (DHS). Adopting the conceptual framework of the Commission on Social Determinants of Health (CSDH), three dimensions related to child health are represented in the index: behavioural factors, psychosocial factors and health system. In order to generate the weight of the variables and take into account the discrete nature of the data, principal component analysis (PCA) using polychoric correlations are employed in the index construction. Weighted multilevel models are used to examine community effects. The results show that the effect of household's SES is attenuated when community characteristics are included, indicating the importance that the level of community development may have in mediating individual and family characteristics. The findings indicate that there is a significant variance in intermediary determinants of child health between-community, especially for those determinants linked to the health system, even after controlling for individual, family and community characteristics. These results likely reflect that whilst the community context can exert a greater influence on intermediary factors linked directly to health, in the case of psychosocial factors and the parent's behaviours, the family context can be more important. This underlines the importance of distinguishing between community and family intervention programmes.
Post-mortem bacterial culture and specific biochemical tests are currently performed to character... more Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. Regarding the analytical sensitivity, DNA of the M. bovis AN5 strain was detected up to 1.5 pg by nested-PCR, whereas DNA of M. tuberculosis H37Rv strain was detected up to 6.1 pg. The nested-PCR system showed 100% analytical specificity for MTC when tested with DNA of reference strains of non-tuberculous mycobacteria and closely-related Actinomycetales. A clinical sensitivity level of 76.7% was detected with tissues samples positive for MTC by means of the culture and conventional PCR. A clinical specificity of 100% was detected with DNA from tissue samples of cattle with negative results in the comparative intradermal tuberculin test. These cattle exhibited no visible lesions and were negative in the culture for MTC. The use of the nested-PCR assay to detect M. tuberculosis complex in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. Table 1 -Bacterial strains used to assess the analytical specificity or sensitivity of the nested-PCR. Bacterial strains Strain /origin Corynebacterium pseudotuberculosis LGCM/FIOCRUZ* Mycobacterium abscessus ATCC 19977/FIOCRUZ Mycobacterium avium ATCC 25291/FIOCRUZ Mycobacterium bovis AN5 strain, Ministry of Agriculture -LANAGRO-MG Mycobacterium fortuitum ATCC 6841/FIOCRUZ Mycobacterium gordonae ATCC 14470/FIOCRUZ Mycobacterium kansasii ATCC 12478/FIOCRUZ Mycobacterium tuberculosis H37Rv/FIOCRUZ Mycobacterium smegmatis ATCC 700044/FIOCRUZ Rhodococcus equi ATCC 6939/FIOCRUZ
En este artículo se estima una línea de pobreza subjetiva (LPS), a través de las respuestas de lo... more En este artículo se estima una línea de pobreza subjetiva (LPS), a través de las respuestas de los hogares colombianos y vallecaucanos a la pregunta de suficiencia de ingresos de la Encuesta de Calidad de Vida 2003 (ECV2003). Los resultados encontrados muestran que un hogar en Colombia se considera pobre si sus ingresos en pesos colombianos son menores a $1.142.097,
The parasitic infection neurocysticercosis may give rise to a variety of psychiatric manifestatio... more The parasitic infection neurocysticercosis may give rise to a variety of psychiatric manifestations that resemble, but are different from, primary psychiatric disorders. The aim of this study was to determine if among individuals from a neurocysticercosis-endemic area of Colombia who apparently had a psychiatric manifestation with associated neurological finding ('cases'), some could have been infected with Taenia solium cysticerci. This case-control study was done in individuals hospitalized in two mental institutions. The control-1 individuals were those classified with primary psychiatric disease, and the control-2 group consisted of healthy, non-hospitalized individuals. A serological test for cysticercosis was positive in 5/96 (5.1%) cases, 4/153 (2.6%) psychiatric controls, and 5/246 (2%) healthy controls. The data analysis indicated a weak association between the cases and a positive serology for neurocysticercosis (odds ratio > 2; P > 0.05). The lower education level of the cases influenced this association.
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance o... more In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT-results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT-animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. Citation: Araú jo CP, Osó rio ALAR, Jorge KSG, Ramos CAN, Filho AFS, et al. (2014) Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. PLoS ONE 9(3): e91023.
The thermodynamic properties of a ternary magnetic system are studied by considering a random-sit... more The thermodynamic properties of a ternary magnetic system are studied by considering a random-site Ising model in the context of the Bogoliubov inequality for the free energy. Only nearest-neighbor sites are considered in order to account for the different interactions in the model. The critical temperature, magnetization, and other quantities have been obtained in an approximation which reproduces the exact results at least in one dimension. The calculations are used to interpret the experimental data of the mean hyperfine field obtained by Mössbauer spectroscopy and the critical temperature obtained by calorimetry measurements of the antiferromagnetic ternary Fe-Mn-Al alloys in the fcc-disordered phase.
Pesq. Vet. Bras. 18(2): 75-82, abr./jun. 1998 75 RESUMO.-Quinze focos de meningoencefalite por he... more Pesq. Vet. Bras. 18(2): 75-82, abr./jun. 1998 75 RESUMO.-Quinze focos de meningoencefalite por herpesvírus bovino-5 (BHV-5) foram diagnosticados entre agosto de 1993 e dezembro de 1996, sendo 14 provenientes do estado do Mato Grosso do Sul e um do estado de São Paulo. A doença ocorreu em diversos municípios e em diferentes épocas do ano. Foram afetados bovinos de 6 a 60 meses de idade, com uma morbidade de 0,05% a 5% e letalidade próxima a 100%.
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