Papers by Amely Zavattieri
Apresentação didáctica sobre o tema "Biotização, feita no INTA Bella Vista, Corrientes, Arge... more Apresentação didáctica sobre o tema "Biotização, feita no INTA Bella Vista, Corrientes, Argentina ao pessoal técnico-científico durante a Sabática de 2015 nessa Instituiçã
Oral presentation Micropropagation of recalcitrant pine (Pinus pinea L.). An overview of the effe... more Oral presentation Micropropagation of recalcitrant pine (Pinus pinea L.). An overview of the effects of ectomycorrhizal inoculation Carla Ragonezi1; Ana Teresa Caldeira2 3; Maria do Rosário Martins2,5; Otília Miralto5,6; Luís Silva Dias6; Elsa Ganhão6, Krystyna Klimaszewska4, Amely Zavattieri5,6 1 Plant Breeding and Biotechnology Laboratory, University of Évora, Ap. 94, 7002-554 Évora, Portugal 2 Department of Chemistry, University of Évora, Ap. 94, 7002-554 Évora, Portugal 3 CQE, University of Évora, Rua Romão Ramalho, nº 59, 7000-671 Évora, Portugal 4 Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy, Québec, QC G1V 4C7, Canada 5 ICAAM, University of Évora, Ap. 94, 7002-554 Évora, Portugal 6 Department of Biology, University of Évora, Ap. 94, 7002-554 Évora, Portugal Corresponding author: Carla Ragonezi email: [email protected] Abstract Stone pine (Pinus pinea L.) is an economically important forest s...
Alqueva is nowadays the most important water reservoir in Portugal. Additionally, it is becoming ... more Alqueva is nowadays the most important water reservoir in Portugal. Additionally, it is becoming an important agronomic and touristic region. These facts bring the questions of the water quality use. During the Alqueva hydro-meteorological experiment (ALEX) campaign, the team of the Water Laboratory from the University of Évora investigated the biological quality indicators of the Alqueva water. Among biological communities investigated, such as Chironomid pupal exuvia, benthic diatoms and phytoplankton, a special attention was given to the presence of cyanobacteria since cyanobacterial blooms are associated with the production of cyanotoxins that pose a health risk to human and livestock water consumers. Polymerase chain reaction (PCR) was used to corroborate microscopic identification and to verify the presence of toxic genes associated with cyanobacteria. Field campaign in Alqueva reservoir took place from June to September 2014. An additional campaign was performed in October du...
Ecological assessment of Mediterranean reservoirs: Alqueva reservoir as a case study (Alentejo, S... more Ecological assessment of Mediterranean reservoirs: Alqueva reservoir as a case study (Alentejo, Southern Portugal) Manuela Morais, Maria Helena Novais, Susana Nunes, Joana Rosado, Alexandra Penha, Amely Zavattieri, Miguel Potes & Rui Salgado Abstract The Mediterranean region, with almost 7% of the world population, has less than 3% of the world freshwater resources, and these are irregularly distributed, 72% in the northern countries (Spain; France; Monaco, Italy, Greece, Malta, Slovenia, Bosnia-Herzegovina, Croatia, Montenegro and Albania) 20 % in the eastern countries (Turkey, Syria, Lebanon, Israel, Palestinian Territories and Cyprus) and the remaining 8% in the Southern countries (Egypt, Libya, Tunisia, Algeria and Morocco) (Aquastat, 2011). Mediterranean Climate Regions occur between 30°- 45° North and South of the Equator, in the west side of continents of the Mediterranean Basin, Middle East California, central Chile, Western Cape in South Africa, and the South West in Wester...
A seguinte apresentação foi apresentada feita durante o Workshop On Observations in atmospheric a... more A seguinte apresentação foi apresentada feita durante o Workshop On Observations in atmospheric and water sciences durante o Projecto Alex. Problemas das Albufeiras do Sul de Portugal e medidas para a sua remediação
LOCUS JQ066791 439 bp DNA linear PLN 20-MAR-2012 DEFINITION Lactarius deliciosus isolate UEZB1 in... more LOCUS JQ066791 439 bp DNA linear PLN 20-MAR-2012 DEFINITION Lactarius deliciosus isolate UEZB1 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. ACCESSION JQ066791 VERSION JQ066791.1 GI:380467948 KEYWORDS . SOURCE Lactarius deliciosus ORGANISM Lactarius deliciosus Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Russulales; Russulaceae; Lactarius
The ALqueva hydro-meteorological EXperiment (ALEX) field campaign took place monthly during summe... more The ALqueva hydro-meteorological EXperiment (ALEX) field campaign took place monthly during summer 2014 and consisted in in situ measurements and sampling of water and biological elements, collected from three fixed platforms placed in the lacustrine zone. This integrated overview, including meteorological, environmental and biological results contributes to improve the knowledge of the reservoir dynamics and therefore to propose adequate management measures to preserve the observed biological integrity.
of somatic embryogenesis as an example of stress-related plant reactions
During the four months of the ALEX 2014, the over water fluxes of momentum, heat and mass (H2O an... more During the four months of the ALEX 2014, the over water fluxes of momentum, heat and mass (H2O and CO2) were obtained with an integrated Open-Path CO2 /H2O Gas Analyser and 3D Sonic Anemometer, mounted on a floating platform (Fig.1) belonging to the Portuguese Environment Agency. The short and long wave, up and down, radiative fluxes were measured at the same place, as well as the water temperature profile. Eight near surface weather stations were operating during the field campaign, two in floating platforms and one in a little island. The air quality, the atmospheric electrical field (Potential Gradient) and the radon (222Rn) concentration were continuous monitored. The solar direct normal irradiation (DNI) as well as other solar radiation components were also measured. Along this period, in situ measurements, water sam-ples and biological elements were monthly collected from three fixed platforms placed in the lacustrine zone and from selected sites in the margins. In each campaign, the vertical profiles of temperature, dis-solved oxygen (mg DO L-1 and %), pH, oxidation-reduction potential, turbidity and solar inwater irra-diation were taken. Simultaneously, water samples were collected at three depths for nutrient determina-tion and microscopic and molecular characterization of cyanobacteria. Diatoms were collected from artificial substrates placed in the platforms and Chironomid pupal exuviae were sampled from two selected sites on the reservoir margins During the IOP, 18 balloons with meteorological radiosondes were launched. In 10 occasions Geigersondes were coupled to the radiosondes in order to obtain the atmospheric ionization profile. The boundary layer was characterized with a Ceilometer and the vertical distribution of O3 and NO2 were obtained from a Spectrometer. A GPS network of 15 GNSS stations was installed in order to map the water vapour. The sky brightness on the nights of July 24 and 25, was measured using a Sky Quality Meter
The ALqueva hydro-meteorological EXperiment (ALEX) field campaign took place monthly during summe... more The ALqueva hydro-meteorological EXperiment (ALEX) field campaign took place monthly during summer 2014 and consisted in in situ measurements and sampling of water and biological elements, collected from three fixed platforms placed in the lacustrine zone. This integrated overview, including meteorological, environmental and biological results contributes to improve the knowledge of the reservoir dynamics and therefore to propose adequate management measures to preserve the observed biological integrity.
Even when stimulated by application of exogenous growth regulators, the ability for ESTUDO HISTOL... more Even when stimulated by application of exogenous growth regulators, the ability for ESTUDO HISTOLÓGICO DE RAÍZES EM ESTACAS CAULINARES DE OLIVEIRA 477 adventitious root formation in olive remains highly genotype dependent. When semi-hardwood cuttings are used as propagation material, it’s possible to find cultivars like ‘Cobrançosa’, where it is easy to achieve 70% of adventitious root formation, and cultivars like ‘Galega vulgar’, where 10% of rooting it is very difficult to exceed. Preliminary results of a comparative histological study between the abovementioned Olea europaea cultivars, looking for differences, both in the stem anatomy and in tissues involved in adventitious root ontogenesis, are now presented. Concerning stem anatomy, the results achieved so far, allow to conclude that no significant differences were observed between the cultivars, both presenting a sclerenchymal ring, that may act as a mechanical barrier to the adventitious root development. The observations ma...
The ALqueva hydro-meteorological EXperiment, ALEX 2014, was an integrated field campaign with mea... more The ALqueva hydro-meteorological EXperiment, ALEX 2014, was an integrated field campaign with measurements of chemical, physical and biological parameters in water and air at different experimental sites in the region of the Alqueva reservoir, a 250 km2 man made lake, in the southeast of Portugal. The Field campaign took place from June 1 to September 30, 2014 and comprises an Intensive Observation Period (IOP) of three days (22 to 24 July). During the four months, the over water fluxes of momentum, heat and mass (H2O and CO2) were obtained with an integrated Open-Path CO2 /H2O Gas Analyser and 3D Sonic Anemometer, mounted on a floating platform, where radiative fluxes were also measured, as well as the water temperature profile. Eight near surface weather stations were operating in the area and air quality, atmospheric electrical field (Potential Gradient) and radon (222Rn) concentration were continuous monitored. Along this period, in situ measurements, water samples and biologica...
IV International Symposium on Acclimatization and Establishment of Micropropagated Plants, 2010
In the present study of Pinus pinea L., further improvement of microshoot rooting was achieved by... more In the present study of Pinus pinea L., further improvement of microshoot rooting was achieved by applying Cool-white light at increased intensity from 60 to 90 µmol m-2 s-1. In contrast, light provided by Gro-lux lamps promoted rooting of the microshoots at the same frequency regardless of its intensity. Majority of microshoots (70.4%) grown under Cool-white lamps at the intensity of 90 µmol m-2 s-1 were also significantly taller when compared with those from other tested treatments.
IV International Symposium on Acclimatization and Establishment of Micropropagated Plants, 2010
Pinus pinea L. (stone pine) is one of the major plantation species in Iberian Peninsula, being Po... more Pinus pinea L. (stone pine) is one of the major plantation species in Iberian Peninsula, being Portugal the largest edible seed producer in the world. The induction and improvement of in vitro rhizogenesis of microshoots of Pinus pinea was developed in our laboratory using a co-culture system with ECM fungi. In the acclimation phase in mixed substrates, or in rhizotrons, anatomical and morphological studies were done to observe the evolution of the root system in microshoots from the co-culture system vs. control plants. Extensive dichotomous and coralloid branching of lateral roots occurred spontaneously in inoculated and control plants as well. Moreover, similar branching occurred in liquid culture of excised seedling roots without the presence of ECM fungi. The striking similarity of these organs with pine ectomycorrhizas prompted their anatomical analysis; however the presence of Hartig net was not confirmed. These results suggested that the development of ECM-like structures might have occurred spontaneously.
Plant Physiology and Biochemistry, 2009
The paper highlights Daucus carota L. as an ideal model to complement plant stress research on Ar... more The paper highlights Daucus carota L. as an ideal model to complement plant stress research on Arabidopsis thaliana L. Recently, alternative oxidase (AOX) is discussed as functional marker candidate for cell reprogramming upon stress. Carrot is the most studied species for cell reprogramming and our current research reveals that it is the only one that has expanded both AOX sub-family genes. We point to recently published, but not discussed results on conserved differences in the vicinity of the most active functional site of AOX1 and AOX2, which indicate the importance of studying AOX sequence polymorphism, structure and functionality. Thus, stress-inducible experimental systems of D. carota are especially appropriate to bring research on stress tolerance a significant step forward.
Trees, 2010
In conifers, vegetative propagation of superior genotypes is the most direct means for making lar... more In conifers, vegetative propagation of superior genotypes is the most direct means for making large genetic gains, because it allows a large proportion of genetic diversity to be captured in a single cycle of selection. There are two aims of vegetative propagation, namely large-scale multiplication of select genotypes and production of large numbers of plants from scarce and costly seed that originates from controlled seed orchard pollinations. This can be achieved, in some species, either through rooted cuttings or rooted microshoots, the latter regenerated through tissue culture in vitro. Thus far, both strategies have been used but often achieved limited success mainly because of difficult and inefficient rooting process. In this overview of technology, we focus on the progress in defining the physical and chemical factors that help the conifer cuttings and microshoots to develop adventitious roots. These factors include plant growth regulators, carbohydrates, light quality, temperature and rooting substrates/media as major variables for development of reliable adventitious rooting protocols for different conifer species. Keywords Cuttings Á Gymnosperms Á In vitro culture Á Micropropagation Á Microshoots Abbreviations ACC 1-Aminocyclopropane-1-carboxylic acid AOA Aminooxyacetic acid ARF Adventitious root formation AVG Aminoethoxyvinylglycine BA 6-Benzyladenine cGMP Cyclic guanosine monophosphate CW Cool white light 123
Plant Growth Regulation, 2011
Juniperus navicularis Gand (= J. oxycedrus L. subsp. transtagana Franco) is an endemic dioecious ... more Juniperus navicularis Gand (= J. oxycedrus L. subsp. transtagana Franco) is an endemic dioecious shrub from the Plio-Plistocene transition sands of the west Portuguese coast line. It grows in sole or mixed stands along with Pinus pinea and P. pinaster and, more rarely, with Quercus lusitanica. In order to reduce fire incidence in these Mediterranean mixed stands, forest management practices of mechanical or chemical treatments, have drastically reduced J. navicularis populations. In addition, the fast expansion of touristic areas along with a low seed germination rate further endangers this species. In this study we developed a protocol for micropropagation of adult J. navicularis. Microcuttings were obtained from lateral and apical twigs of both female and male mature plants, and used as explants. Microcuttings with axillary buds were grown on different media and plant growth regulators combinations. Developmental parameters were evaluated to define the best medium and plant growth regulator concentration for shoot bud induction during the multiplication cycles. Olive Medium and Gupta and Durzan medium, both supplemented with 0.45 µM 6-benzylamino-purine, were the most favourable of all combinations tested achieving more than 3 new shoots per explant. The highest rooting (60%) was obtained in microshoots cultured in Olive Medium when supplemented with indole-3-butyric acid at 12.3 μM. With this multiplication protocol, it was possible to obtain more than 500 shoots from the initial 20, after three multiplication cycles in three months.
Physiologia Plantarum, 2009
The gymnosperm Pinus pinea L. (stone pine) is a typical Mediterranean pine used for nuts and timb... more The gymnosperm Pinus pinea L. (stone pine) is a typical Mediterranean pine used for nuts and timber production, and as an ornamental around the world. Pine genomes are large in comparison to other species. The hypothesis that retrotransposons, such as gymny, made a large contribution to this alteration in genome size was recently confirmed. However, P. pinea is unique in other various aspects. P. pinea demonstrates a different pattern of gymny organization than other Pinus subgenera. Additionally, P. pinea has a highly recalcitrant behaviour in relation to standard conifer protocols for the induction of somatic embryogenesis or rooting. Because such types of cell reprogramming can be explained as a reaction of plant cells to external stress, it is of special interest to study sequence peculiarities in stress-inducible genes, such as the alternative oxidase (AOX). This is the first report containing molecular evidence for the existence of AOX in gymnosperms at the genetic level. P. pinea AOXs were isolated by a polymerase chain reaction (PCR) approach and three genes were identified. Two of the genes belong to the AOX1 subfamily and one belongs to the AOX2 subfamily. The existence of both AOX subfamilies in gymnosperms is reported here for the first time. This discovery supports the hypothesis that AOX1 and AOX2 subfamilies arose prior to the separation of gymnosperms and angiosperms, and indicates that the AOX2 is absent in monocots because of subsequent gene loss events. Polymorphic P. pinea AOX1 sequences from a selected genetic clone are presented indicating non-allelic, non-synonymous and synonymous translation products.
Citação: Ragonezi, C., Klimaszewska, K., Santos-Silva, C., Martins, MR, Caldeira, AT, Zavattieri,... more Citação: Ragonezi, C., Klimaszewska, K., Santos-Silva, C., Martins, MR, Caldeira, AT, Zavattieri, A. 2011. Pisolithus sp. P1001 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8 S ribosomal RNA gene, and internal transcribed spacer ...
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Papers by Amely Zavattieri