Papers by Alireza Sadeghipour
PubMed, 2022
One rare and low-grade soft tissue tumor with intermediate malignant potential is angiomatoid fib... more One rare and low-grade soft tissue tumor with intermediate malignant potential is angiomatoid fibrous histiocytoma (AFH)و which occurs mainly in children and adolescents. The tumor naturally tends to local recurrence and recurrent hemorrhage but rarely to remote metastasis. AFH has been reported in different organs; however, there are rare reports of primary intracranial AFH. The diagnosis of AFH may be difficult due to its occurrence at multiple unusual anatomic sites and its spectrum of morphologic patterns; thus, it is especially important to diagnose it correctly because of the small risk of metastasis and death. The lesion is simply confused with a hematoma, soft tissue hemangioma, or malignant fibrous histiocytoma from clinical and radiographical aspects. We report a case of intracranial AFH in a 5-year-old boy. The tumor is a heterogeneous intra-axial with a size of 78*73mm at the right front temporal. There was also an extra-axial mass measured 8*12mm at the left superior frontal lobe in favor of metastasis. The diagnosis was confirmed using radiographical, immunohistochemical, and molecular tests. AFH is a rare tumor with a high probability of misdiagnosis. Surgeons must be aware of the presence of AFH and conduct a careful follow-up.
Research Square (Research Square), Sep 6, 2022
Introduction: Chronic lymphocytic leukemia (CLL) is de ned by the presence of lymphocyte in the b... more Introduction: Chronic lymphocytic leukemia (CLL) is de ned by the presence of lymphocyte in the blood, bone marrow, lymph nodes, and spleen, with a median lymphocyte count of 20-30×10 9 /L for more than three months. CLL is known to be rarely associated with myeloid malignancies. This study presents an extremely rare case of CLL, which transformed into myeloid /T neoplasm. Case presentation: A 62-year-old man with a 10-year history of CLL was referred to our hospital because of multiple skin bruises and anemia. He was monitored for ten years without any treatment until this time when the disease progressed, and chemotherapy was started. After eight months of treatment completion, he deteriorated. This time, bone marrow biopsy immunophenotyping revealed the transformation of CLL into mixed phenotype acute leukemia, myeloid/T neoplasm with no residue of preexisting CLL. Conclusion: Transformation of CLL into acute leukemia may arise from the same clone. Still, there is some evidence that it may be therapy-related, so any deterioration in a patient with CLL should be evaluated promptly for a better outcome.
Background and Objectives: Epstein Barr Virus (EBV) is one of the members of herpesviridae family... more Background and Objectives: Epstein Barr Virus (EBV) is one of the members of herpesviridae family and a sub-category of Gamma herpes virinae. EBV, which normally has CR2 or CD21 receptors on B-lymphocytes, has mutagenic features for them. The virus plays an important role in causing some malignant cancers. About 30% of the cases with non-Hodgkin's lymphoma are diffuse large B-cell lymphoma (DLBCL). In the present study, the incidence rate of EBV in DLBCL was evaluated. Materials and Methods: Immunohistochemistry (IHC) and PCR methods were used for studying the relationship between EBV and DLBCL. Paraffin blocks of 116 patients from Sina & Shariati hospitals, Tehran, Iran, with DLBCL diagnoses in 2005-2009 were collected. EBV-LMP in IHC and PCR virus genome in PCR were examined. Results: Findings of the PCR method showed that 28 cases of the total 116 patients with DLBCL were EBV positive (the frequency of EBV positivity was correspondingly 40% and 60% in females and males) and this shows a 25.8% EBV frequency in DLBCL. IHC findings showed that six cases were EBV positive. The compatibility of positive IHC and PCR responses was two cases and there are four conflicting cases. Conclusion: It seems that PCR is a more appropriate method for diagnosing EBV and IHC cannot solely prove the presence of EBV in DCBCL patients.
PubMed, Oct 1, 2016
Leiomyosarcoma is one of the soft tissue sarcomas that could originate from different parts of bo... more Leiomyosarcoma is one of the soft tissue sarcomas that could originate from different parts of body and are mostly presented as retropritoneal mass. Leiomyosarcomas of vascular origin are particularly rare tumors occurring mainly in inferior vena cava (IVC). Here, we report the case of a 35-year-old male patient who presented with ascites and jaundice. Further evaluation revealed large hepatic and cardiac masses with extension to IVC. Since it was not possible to determine the point of origin of leiomyosarcoma, the patient received chemotherapy under diagnosis of metastatic leiomyosarcoma but unfortunately passed away.
Different parts of the Salvia officinalis L. (sage) (Labiatae) are used to treat liver disorders,... more Different parts of the Salvia officinalis L. (sage) (Labiatae) are used to treat liver disorders, traditionally. It is one among the constituents in various folk medicines used for the treatment of liver disorders and other diseases. The aim of the present study is to evaluate the protective effect of Salvia officinalis against experimentally induced liver injury. The methanolic extract of aerial parts of Salvia officinalis was evaluated for the hepatoprotective activity against Aspergillus parasiticus aflatoxin induced hepatotoxicity in rats. Various biochemical parameters like serum alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (AP) and total protein (TP) levels were determined. The treatment of aflatoxin at dose 480 µg/kg increased serum ALT, AST and AP levels, while decreased total protein levels in contaminated rats in comparison to control normal rats. Treatment of sage extract at doses 25, 50, 100 and 150 mg/kg mg/kg body weight decreased the raise of serum AST, ALT and AP levels and increased serum total protein level in treated rats in comparison to control rats. This study demonstrates the hepatoprotective activity of Salvia officinalis and thus scientifically supports the usage of this plant in traditional medicine for treatment of liver disorders.
Razi Journal of Medical Sciences, Sep 15, 2005
Clinical Case Reports, Nov 11, 2020
Mesenteric cysts (MCs) are rare benign lesions found in the abdomen with an incidence rate of 1 p... more Mesenteric cysts (MCs) are rare benign lesions found in the abdomen with an incidence rate of 1 per 100 000 adults and 1 per 20 000 children. Although it can be found at all ages, it is more common among adults at the age of between 40 and 70 years and children under 10 years without any gender preference. While their typical sizes are up to 10 cm, they can emerge in larger sizes. However, very large mesenteric cysts are rare presentations of such benign tumors. 1,2 This lesion was first diagnosed and introduced by Benineni, an Italian anatomist, in an eight-year-old boy, in 1507. Afterward, in 1850, Paul Jules Tillaux successfully resected a mass of this type of cyst for the first time. Subsequently, marsupialization of the cyst was conducted by Pean in 1883. 3 The mesenteric cysts exhibit no signs in 40% of cases. They are usually diagnosed accidentally, while tumor expansion, mesenteric stretching, or pressure on surrounding organs may cause symptoms such as abdominal pain, intestinal obstruction, nausea, and vomiting. 1 Ganglioneuromas (GNs) are benign tumors in sympathic system, originating from neural crest cells. They are composed of ganglionic cells, Shawn cells, and neurites. Generally, this tumor is found in posterior mediastinum and retroperitoneum. In addition, there are a few rare cases of its formation in the adrenal medulla. GN tumors can be found at all ages. However, the rare cases of GN tumor of the adrenal gland are more common in children and young adults. GNs are often hormonally silent and present no symptom; hence, the clinical diagnosis is difficult and virtually in all cases accidental. 4
PubMed, Sep 1, 2013
Introduction: BIOMED-2 multiplex polymerase chain reaction (PCR) protocol is a widely accepted to... more Introduction: BIOMED-2 multiplex polymerase chain reaction (PCR) protocol is a widely accepted tool for evaluation of clonality in lymphoma diagnosis. Diffuse large B-cell lymphoma (DLBCL) is the most common non- Hodgkin,s lymphoma and displays a special challenge for PCR-based clonality analysis due to a high frequency of somatic hypermutation in rearranged immunoglobulin (Ig) domains. In this study, we evaluated detection of B- cell clonality in DLBCL by using Ig heavy chain (IgH) framework region 3 (FR3) primers in formalin- fixed paraffin- embedded (FFPE) tissue. Method: FFPE samples from 100 cases diagnosed as DLBCL in the period of 2005 through 2011 were assessed in this study. Clonality of IgH (FR3) was evaluated by PCR amplification method that was optimized for FFPE tissue. Results: The clonal detection rate was (62.8%) with IgH (FR3) assay after modification, using filter for better DNA purification on negative cases. DNA quality in FFPE samples stored in recent five years were significantly better than older paraffin blocks (P < 0.001). Although a higher rate of clonality was observed in more recent group, it was not statistically significant. Conclusion: By using IgH (FR3) primers followed by one additional filter tube for better DNA purification, we could achieve a considerable rate of clonality with little adverse impact of DNA degradation.
International Journal for Vitamin and Nutrition Research, Nov 1, 2019
. Introduction: The effect of using high dose pomegranate extract on sepsis and its safety is not... more . Introduction: The effect of using high dose pomegranate extract on sepsis and its safety is not clarified. Considering the fact that proper immune and inflammatory responses are needed to cope with infection, the aim of current study was to assess the effect of high dose pomegranate extract consumption on oxidative and inflammatory responses after disease induction in rat model of sepsis. Methods: Sepsis was induced by Cecal Ligation and Perforation (CLP) surgery. Adult male Wistar rats were divided into three groups of eight animals: Sham; CLP and POMx [consumed POMx (250 mg of pomegranate fruit extract/kg/day) for four weeks before CLP]. Results: Peritoneal neutrophil myeloperoxidase activity was significantly lower in POMx compared with Sham and CLP groups ( p &lt; 0.01 and p &lt; 0.05, respectively). Although antioxidant enzymes were higher in POMx group after sepsis induction, lower serum total antioxidant status (TAS) (p &lt; 0.01 compared with both CLP and Sham groups) and higher liver thiobarbituric acid reactive species (TBARS) levels were observed in this group ( p &lt; 0.01 and p &lt; 0.05, compared with Sham and CLP groups, respectively). Conclusion: High dose POMx consumption prior to sepsis induction, suppressed the vital function of neutrophils in early hours after sepsis initiation, resulting in higher oxidative stress. These findings indicate that caution should be made in using high dose pomegranate products. The main message of current study is that such useful compounds as antioxidants including pomegranate juice which have beneficial effects on general health status may have detrimental effects if misused or used in high doses.
Medical journal of the Islamic Republic of Iran, Apr 30, 2021
A critical point for better management of COVID-19 is: knowing more about exact pathomechanisms o... more A critical point for better management of COVID-19 is: knowing more about exact pathomechanisms of events in different organs during disease course and COVID-related histopathological events like tissue sampling, either biopsy or necropsy/autopsy of died people, collecting from different populations. →What this article adds: In lung necropsy of 15 Iranian patients, we found similar presentation of histopathologic events compared with previous similar studiers: A high percentage of anthracosis and viral cytopathic effects, and a lower percentage of microthrombotic vascular lung injuries, suggesting some probable differences in pathologic events among different populations.
Ear, nose, & throat journal, Oct 1, 2007
Primary giant-cell tum or of the salivary gland is a rare lesion with an incompletely characteriz... more Primary giant-cell tum or of the salivary gland is a rare lesion with an incompletely characterized histogenesis. To the best of our kno wledge, only 16 cases have been previously documented in the Engli sh-lan gua ge literatu re. We report a new cas e, which occurred in a 75-year-old man who presented with a parotid mass and cervica l lymphadenopathy. The patient underwent a lef t total parotidectomy and cervical lymph node dissection. As far as we know, ours is the only reported case of a primary giant-cell tumor of the salivary gland in whic h the pati ent presented with lymph node metastasis. Because so little is known about giant-cell tumor ofthe salivary gland, we use the occasion of this case report to describ e the cytologic, histologic, and immun ohistochemical characteristics that we observed.
Journal of Clinical Oncology, May 20, 2008
Methods in molecular biology, Dec 12, 2018
Paraffin embedding is a standard technique used in clinical and research laboratories to create a... more Paraffin embedding is a standard technique used in clinical and research laboratories to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue. Formalin-fixed tissue undergoes tissue processing and then is embedded in paraffin (wax) to create a FFPE block or paraffin block. The paraffin block can be cut using a microtome to generate thin sections of tissue contained in paraffin to be stained or paraffin tissue ribbons suitable for nucleic acid extraction. In addition, the FFPE blocks can be stored at room temperature for years. Herein, we provide a basic knowledge, and introduce common methods of the paraffin embedding process.
Iranian Red Crescent …, 2010
Annals of Saudi Medicine, May 1, 2005
Multiple sclerosis and related disorders, Aug 1, 2020
The new severe acute respiratory syndrome-coronavirus 2 is reported to affect the nervous system.... more The new severe acute respiratory syndrome-coronavirus 2 is reported to affect the nervous system. Among the reports of the various neurological manifestations, there are a few documented specific processes to explain the neurological signs. We report a para-infectious encephalitis patient with clinical, laboratory, and imaging findings during evolution and convalescence phase of coronavirus infection. This comprehensive overview can illuminate the natural history of similar cases. As the two previously reported cases of encephalitis associated with this virus were not widely discussed regarding the treatment, we share our successful approach and add some recommendations about this new and scarce entity.
Iranian Journal of Allergy Asthma and Immunology, Oct 15, 2018
Despite many years having passed since exposure to sulfur mustard (SM) gas, there are many expose... more Despite many years having passed since exposure to sulfur mustard (SM) gas, there are many exposed subjects who are still suffering from delayed pulmonary complications. The levels of pro-inflammatory cytokines in the lung of these subjects have not been investigated in delay phase. In this study, we evaluated mRNA expression of pro-inflammatory cytokines (tumor necrosis factor alpha (TNFα), tumor necrosis factor receptor type 1 (TNFR1), and interleukin 1 beta (IL-1β)) in lung biopsy of SM-exposed subjects and compared them with control (non-exposed) subjects. We used formalin-fixed, paraffin-embedded (FFPE) tissue for this purpose. Lung FFPE blocks of SM-exposed subjects (30 samples) and a control group (30 samples) were collected from archival pathology department. The total mRNA of FFPE tissues were extracted and the mRNA expression of pro-inflammatory cytokines were determined by quantitative Real Time PCR (RT-qPCR). The obtained results from two groups were compared to each other and non-parametric statistical analyses were carried out on them. Our studies showed that the mRNA expression of TNFα, TNFR1 and IL-1β in lung tissue of SM injured and control people have no significant difference (p-value= 0.159, 0.832 and 0.314 respectivly). TNFR1 showed direct correlation with TNFα (r=0.867, p=0.002) and IL-1β (r= 0.65, p=0.006). The evaluation of mRNA expression in pro-inflammatory cytokines in lung of SMexposed subjects after 20 years showed that these mediators are similar to those of nonexposed group and there was no acute inflammation in lung of these patients.
Jcr-journal of Clinical Rheumatology, Feb 27, 2021
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Papers by Alireza Sadeghipour