Indonesian Food Science and Technology Journal, Dec 29, 2022
Detection of pathogenic bacteria Listeria monocytogenes in processed food products of frozen meat... more Detection of pathogenic bacteria Listeria monocytogenes in processed food products of frozen meatballs is one of the critical test parameters in product quality testing. The challenge in testing product quality is how to find a reliable and effective test method to generate test results data. With this research, it is hoped that it can provide information or references for similar research. The method used in this research is the enrichment method with a direct isolation technique and amplification using real-time PCR. Data analysis is based on the Ct (Cycle threshold) and Tm (Melt Curve) values. The results of the tested samples were detected at Ct 11.60 with a Tm value of 81.50. For NTC control not detected, this indicates that the master mix used is in good condition and no contamination occurred when testing the sample. The use of this NTC control plays an important role in monitoring the quality of the master mic and test run. For the LOD control and positive control, the results were detected, whereas in the LOD control, the Ct value was detected at 10.08 and the Tm value was at 81.30. for positive control, the value of Ct was detected at 10.85 and the value of Tm was at 82.0. Based on the results of the study, it can be concluded that the test method used can detect Listeria monocytogenes in processed meat products in the form of frozen meatballs.
The development of pathogenic bacteria detection methods in food products has led to the emergenc... more The development of pathogenic bacteria detection methods in food products has led to the emergence of faster, shorter, and more efficient techniques. However, when choosing a reference method for testing, it is crucial to ensure its re liability. The purpose of this study was to determine the limit of detection (LOD) for Salmonella testing in processed chicken egg food products (Pindang Egg). The research aims to provide valuable information and serve as a reference for similar studies. The method used in this study follows ISO 6579-1:2017, while the detection limit is determined according to ISO 16140-3:2021. The results from observations on pre-enrichment and enrichment media showed that all inoculated samples exhibited a color change to cloudy, indicating bacterial growth in the media. Isolation on selective media and conformational tests confirmed 100% positivity for all samples in contamination variations of 11, 3.67, and 1.22. However, for contamination variation of 0.41, only 75% of the anal yzed test data detected the presence of Salmonella, with 1 out of 4 replications not detecting it (approximately 20% failure rate). In conclusion, the lowest LOD value that can be reliably detected is 100% in the contamination variation of 1.22, while for the 0.41 variation, the detection rate only reaches 75% of the analyzed test data.
Asian Journal of Tropical Biotechnology, Jun 9, 2021
Sophian A. 2021. Short Communication: Analysis of purity and concentration of extracted DNA on sa... more Sophian A. 2021. Short Communication: Analysis of purity and concentration of extracted DNA on salted fish processed food products. Asian J Nat Prod Biochem 19: 21-24. Analysis of purity and concentration of extracted DNA on salted fish processed food products was carried out in the microbiology and molecular biology testing laboratory of the Food and Drug Administration in Gorontalo. The purpose of this study was to analyze the extracted DNA on salted fish processed food products based on the concentration and purity values in the A260 / A230 and A260 / A280 wavelengths. The method used for purity and concentration analysis was the absorbance method using a nanophotometer. The samples used were 10 types of salted fish processed food products sampled from 5 (five) traditional markets in Gorontalo City. The sample was extracted using the spin column method with the Dneasy Mericon Food Kit (50) paint kit. 69514 (Qiagen). The research data showed that extracted sample concentration was in the range of 24,600-27,150 with an average of 25,745, while purity value measured at A260 / A280 wavelength was obtained with a purity range between 1,668-1,768 with an average of 1,729. Based on the results of this study, it can be concluded that the results of DNA extraction carried out on salted fish processed food products show a value that is in the category of good DNA extraction results.
DNA detection of porcine species in processed meat samples (shredded meat) was carried out as an ... more DNA detection of porcine species in processed meat samples (shredded meat) was carried out as an effort to enrich the literature and reference alternative testing methods in the control of halal products circulating in Indonesia. The purpose of this study was to detect the DNA of porcine species in samples using real-time PCR. The research method used is a qualitative technique. This technique analyzes the CT value of the sample compared to the positive control by looking at the formation of the sigmoid curve if amplification occurs. The controls used consisted of negative control, positive control, other DNA control, and LOD control. The extraction technique used is the column centrifuge technique. The extraction results were then measured using a nano photometer to see the purity and concentration of the extracted DNA. Based on the research, it is known that the extraction results read on a nanophotometer at a wavelength of A260 / A280 show a concentration of 24,350 with a purity ...
HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY
The authentication test for spices made from turmeric using real-time. This study aimed to determ... more The authentication test for spices made from turmeric using real-time. This study aimed to determine whether the spice ingredients claimed to be made from turmeric actually contain turmeric, as asserted by the product. The sample set consisted of 10 types of spices based on turmeric, which were obtained from various supermarkets. The research employed real-time PCR analysis using the SYBR Green method as the chosen approach. Data analysis was performed based on Ct analysis and Tm analysis. The measurement of DNA purity and concentration resulted in a DNA concentration value of 56.205 and a DNA purity value of 2.302, measured at a wavelength of A260/A280. The real-time PCR analysis yielded Ct values ranging from 15.20 to 15.83 with an average of 15.50, and Tm values ranging from 78.70 to 89.00 with an average of 78.83. Based on these data, it can be concluded that the ready-to-use spice products, claimed to be made from genuine turmeric, indeed contain turmeric, as supported by the r...
Analysis of the purity and concentration of DNA isolation results was carried out using samples o... more Analysis of the purity and concentration of DNA isolation results was carried out using samples of dragonflies (Onychogomphus forcipatus) species of insects. This research is important because there are many kinds of research in the molecular field that require good DNA isolation techniques to support the success of the research. The purpose of this paper is to provide information about the quality of the isolated DNA seen using the purity and concentration parameters. The extraction method uses the centrifuge column method. The isolated data were analyzed statistically using the average test. The results of DNA isolation showed that the average concentration value was in the range of 60.10 – 69.95. The analysis of the purity of the isolated DNA read at the A260/A280 ratio was in the range of 1.817 – 1.929, while the purity analysis at the A260/A230 ratio was in the range of 0.760 – 0.822. Based on the results of the study, it can be concluded that the analysis of the purity and con...
HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY
Real-Time PCR is a molecular biology testing instrument used for various types of food product te... more Real-Time PCR is a molecular biology testing instrument used for various types of food product testing applications to monitor the quality of a product. In addition, the real-time PCR technique can also be used to test the detection of pathogenic bacteria in some foods and processed products. The purpose of this study was to detect Salmonella typhimurium using real-time PCR. This research can be a source of information and a reference for similar research. The test method used is the real-time PCR method with the green SYBR kit. Data analysis was carried out by looking at the results of interpreting Ct values and Tm values. Positive results are indicated by the formation of the Ct curve and the Tm curve. From the research conducted, it was found that the Ct value was in the range of 11.90 - 12.10 with an average of 12.02, while the Tm value was in the range of 85.10 - 85.20 with an average of 85.15. From the study results, it can be concluded that the use of real-time PCR for testin...
Analysis of the purity and concentration of isolated DNA is a way to see the quality of isolated ... more Analysis of the purity and concentration of isolated DNA is a way to see the quality of isolated DNA. This research was conducted as an initial stage to give. Information about research conducted in future research. The purpose of this research is to provide references and information regarding DNA isolation techniques in chondroitin samples so that they can be used in similar studies. The sample consisted of a sample of chondroitin which was weighed 50 mg and tested 10 times. The isolation method used in this study is the centrifuge column isolation method, while the purity and concentration analysis were analyzed using a nano photometer which was read at a wavelength of A260/A280. The value shown from the nano photometer was then analyzed statistically using the average test to determine the interpretation of the results from the data obtained. The results of DNA isolation obtained that the DNA concentration values tested were in the range of 39.10-54.70 with an average concentration value of 45.15. The value of the purity of the isolated DNA tested was in the range of 2.16-2.28 with an average purity value of 2.22. Based on the results of the DNA confirmation test of the isolation carried out using real-time PCR, it showed that the isolated sample was amplified at a value of Ct 36.43 while the positive control was amplified at Ct 32.49. Based on the research results, it was found that all samples tested showed good average values of DNA concentration and purity so that the results of the DNA isolation tested could be used as templates in real-time PCR analysis.
Analisis mutu DNA untuk melihat kualitas DNA hasil isolasi merupakan sebuah teknik analisis yang ... more Analisis mutu DNA untuk melihat kualitas DNA hasil isolasi merupakan sebuah teknik analisis yang dapat digunakan untuk mendukung pengujian bidang biologi molekuler yang menggunakan instrumen PCR atau real-time PCR. Tujuan dari penelitian ini adalah untuk menganalisis kualitas DNA hasil isolasi pada sampel produk pangan olahan ikan. Manfaat penelitian ini diharapkan dapat menjadi sumber informasi yang mendukung penelitian di bidang molekuler pada ruang lingkup pengawasan mutu dan autentifikasi pada produk pangan olahan ikan. Analisis DNA hasil isolasi dianalisis menggunakan NanoPhotometer untuk mengukur nilai konsentrasi dan kemurnian dengan panjang gelombang A260/A280. Data yang diperoleh dari hasil pengukuran kemudian diinterpretasikan menggunakan nilai uji rata-rata untuk menganalisis mutu DNA hasil isolasi. Data hasil penelitian menunjukan nilai konsentrasi DNA berada pada kisaran 14,5 – 17,8 dengan rata-rata nilai konsentrasi berada pada 16,1. Untuk nilai kemurnian DNA hasil is...
Muhammadiyah Journal of Nutrition and Food Science (MJNF)
Latar belakang: Parameter nilai kemurnian yang dianalisis dari panjang gelombang dengan rasio 260... more Latar belakang: Parameter nilai kemurnian yang dianalisis dari panjang gelombang dengan rasio 260/230 merupakan parameter validasi sekunder yang digunakan untuk melakukan analisis mutu DNA hasil isolasi pada produk nugget ayam. Tujuan: Tujuan dari dilakukannya penelitian ini adalah untuk memberi informasi tentang nilai analisis kemurnian DNA hasil isolasi yang dibaca pada rasio 260/230. Dengan diperolehnya informasi dari hasil penelitian ini diharapkan dapat memberi manfaat terhadap penelitian sejenis yang relevan. Metode: Metode yang digunakan dalam penelitian ini adalah metode isolasi DNA menggunakan teknik spin kolom, kemudian DNA hasil isolasi yang diperoleh dibaca menggunakan nano fotometer pada rasio 260/230. Hasil: Hasil pengukuran kemudian dihitung nilai rata-rata dan standar deviasinya. Berdasarkan hasil pengukuran DNA hasil isolasi diperoleh hasil kemurnian yang dibaca pada rasio A260/A230 berada pada kisaran 1,98 – 2,10, dengan nilai rata-rata berada pada nilai 2,043. S...
HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY
DNA isolation is one of the important steps in conducting PCR-based or real-time PCR-based molecu... more DNA isolation is one of the important steps in conducting PCR-based or real-time PCR-based molecular analysis. This study aims to test the quality of DNA isolation extracted from samples of processed food products from chicken meat (chicken nuggets). The novelty of this research lies in the DNA isolation technique modified from the manual kit which is used to produce good-quality DNA isolation results. The extraction method used was the modified extraction method and the standard extraction method of the DNeasy Mericon Food kit. Analysis of DNA quality was measured using a nanophotometer, where the analysis of DNA quality was analyzed based on the parameters of concentration values and purity values. Based on research data, it is known that the modified DNA extraction method produces a concentration value of DNA isolation with an average concentration value of 1295.1 ng/µL. Meanwhile, the value of the purity of the isolated DNA was 2.10 on average. while the standard DNA extraction ...
The myostatin (MSTN) gene provides instructions for making myostatin, which helps control the gro... more The myostatin (MSTN) gene provides instructions for making myostatin, which helps control the growth and development of tissue throughout the body and limits muscle growth. Myostatin has been studied in mice, cows, and other animals. However, the MSTN gene has not been studied in the nisi chicken. Further, the lack of information using the real-time polymerase chain reaction (PCR) to detect the MTSN gene is still to be explored. Accordingly, this study aimed to detect the MSTN gene in nisi chickens, one of the local-endemic Indonesian chickens, using real-time PCR. DNA of twenty-five male chickens was extracted using an automatic DNA extraction tool, and the MSTN gene was analyzed by real-time PCR Rotor-Gene 5 Plex. Ct and Tm values were indicators of the successful amplification of the MSTN gene. The results showed that the MSTN gene was detected by Ct 16.00 and Tm 80.00, respectively. The study novelty is based on the nisi chicken as the local-endemic chicken from Indonesia as the...
BiosciED: Journal of Biological Science and Education
Escherichia coli bacteria test on contaminated meat processed food products with several variatio... more Escherichia coli bacteria test on contaminated meat processed food products with several variations of positive control concentrations was carried out to provide additional information about determining the LOD value in the test method for the detection of Escherichia coli pathogenic bacteria. The purpose of this study was to detect and identify bacterial contamination of Escherichia coli ATCC 25922 in contaminated meat processed food products with variations in the concentration of positive control. The method used to identify is the enrichment method, using enrichment media to grow the suspected target bacterium Escherichia coli ATCC 25922 spiked in meat-processed food products, followed by isolation using selective media and ending with confirmation and affirmation tests. The samples used were 10 packages of processed meat food products. The samples were then spiked using various concentrations of positive control Escherichia coli 5, 10, 50, and 100 colonies/gr. The data from the...
Eruditio : Indonesia Journal of Food and Drug Safety
The presence of gelatin components can be identified through fat, protein and DNA. DNA can be obt... more The presence of gelatin components can be identified through fat, protein and DNA. DNA can be obtained through the DNA isolation process which is the process of separating DNA from other components of the cell. The DNA purification stage is the most important thing in DNA isolation because at this stage it can reduce to a minimum the number of contaminants in DNA isolates to determine the purity and concentration of the DNA isolate obtained. The purpose of this technique is to produce DNA isolates that are low inhibitors or are in the purity range of 1.7-2.1. The extraction method used in this study used the double wash technique, the technique was modified from the test stage following the manual kit used, it's just that the technique used was modified at the washing stage with each washing done twice in the washing section. first and second washing so that a total of four items of washing were carried out. The results of the DNA isolates were then analyzed for the purity and c...
Detection of Salmonella typhimurium ATCC 14028 bacteria in bakery product samples by real-time PC... more Detection of Salmonella typhimurium ATCC 14028 bacteria in bakery product samples by real-time PCR using boiling isolation technique. The basis of this research is to have an impact on economic value in carrying out the confirmation test for Salmonella typhimurium ATCC 14028, where testing is carried out conventionally will require large costs, so it is necessary to innovate in terms of modifying the testing phase so that it is more effective and efficient. The purpose of this study was to see whether the boiling isolation technique could be used for the detection test for Salmonella typhimurium ATCC 14028 on bacterial product samples. The sample in this study consisted of 15 types of bacterial product samples spiked with Salmonella typhimurium ATCC 14028 cultures that had been cultured into phase 2 working cultures. The method used in this study was qPCR analysis using the SYBR Green method. The results of real-time PCR analysis obtained Ct values in the range 7.55 - 8.91 with an a...
Species DNA detection using genetic markers of the PGR gene on chicken nuggets was carried out to... more Species DNA detection using genetic markers of the PGR gene on chicken nuggets was carried out to see whether these genetic markers could be used to test species DNA detection in chicken nuggets food products. The test method used in this study is a real-time PCR test method using the SYBR green technique where the test results can be either Ct or Tm values which indicate amplification or detection. The results of DNA isolation showed that the concentration and purity of the isolated DNA were in the range of 35.10 ng/µL – 36.10ng/µL with an average of 35,488 ng/µL. As for the purity value measured at the A260/A280 wavelength, the results were obtained with a purity range between 2,110 – 2,250 with an average of 2,165. For the results of real-time PCR amplification, the Ct value of the chest sample was at 24.50, the Ct LOD value was at 21.20 and the Ct value of the positive control was 27.10. For the Tm value of the busty sample at 81.10, the Tm LOD value at 81.20 and the positive ...
Indonesian Food Science and Technology Journal, Dec 29, 2022
Detection of pathogenic bacteria Listeria monocytogenes in processed food products of frozen meat... more Detection of pathogenic bacteria Listeria monocytogenes in processed food products of frozen meatballs is one of the critical test parameters in product quality testing. The challenge in testing product quality is how to find a reliable and effective test method to generate test results data. With this research, it is hoped that it can provide information or references for similar research. The method used in this research is the enrichment method with a direct isolation technique and amplification using real-time PCR. Data analysis is based on the Ct (Cycle threshold) and Tm (Melt Curve) values. The results of the tested samples were detected at Ct 11.60 with a Tm value of 81.50. For NTC control not detected, this indicates that the master mix used is in good condition and no contamination occurred when testing the sample. The use of this NTC control plays an important role in monitoring the quality of the master mic and test run. For the LOD control and positive control, the results were detected, whereas in the LOD control, the Ct value was detected at 10.08 and the Tm value was at 81.30. for positive control, the value of Ct was detected at 10.85 and the value of Tm was at 82.0. Based on the results of the study, it can be concluded that the test method used can detect Listeria monocytogenes in processed meat products in the form of frozen meatballs.
The development of pathogenic bacteria detection methods in food products has led to the emergenc... more The development of pathogenic bacteria detection methods in food products has led to the emergence of faster, shorter, and more efficient techniques. However, when choosing a reference method for testing, it is crucial to ensure its re liability. The purpose of this study was to determine the limit of detection (LOD) for Salmonella testing in processed chicken egg food products (Pindang Egg). The research aims to provide valuable information and serve as a reference for similar studies. The method used in this study follows ISO 6579-1:2017, while the detection limit is determined according to ISO 16140-3:2021. The results from observations on pre-enrichment and enrichment media showed that all inoculated samples exhibited a color change to cloudy, indicating bacterial growth in the media. Isolation on selective media and conformational tests confirmed 100% positivity for all samples in contamination variations of 11, 3.67, and 1.22. However, for contamination variation of 0.41, only 75% of the anal yzed test data detected the presence of Salmonella, with 1 out of 4 replications not detecting it (approximately 20% failure rate). In conclusion, the lowest LOD value that can be reliably detected is 100% in the contamination variation of 1.22, while for the 0.41 variation, the detection rate only reaches 75% of the analyzed test data.
Asian Journal of Tropical Biotechnology, Jun 9, 2021
Sophian A. 2021. Short Communication: Analysis of purity and concentration of extracted DNA on sa... more Sophian A. 2021. Short Communication: Analysis of purity and concentration of extracted DNA on salted fish processed food products. Asian J Nat Prod Biochem 19: 21-24. Analysis of purity and concentration of extracted DNA on salted fish processed food products was carried out in the microbiology and molecular biology testing laboratory of the Food and Drug Administration in Gorontalo. The purpose of this study was to analyze the extracted DNA on salted fish processed food products based on the concentration and purity values in the A260 / A230 and A260 / A280 wavelengths. The method used for purity and concentration analysis was the absorbance method using a nanophotometer. The samples used were 10 types of salted fish processed food products sampled from 5 (five) traditional markets in Gorontalo City. The sample was extracted using the spin column method with the Dneasy Mericon Food Kit (50) paint kit. 69514 (Qiagen). The research data showed that extracted sample concentration was in the range of 24,600-27,150 with an average of 25,745, while purity value measured at A260 / A280 wavelength was obtained with a purity range between 1,668-1,768 with an average of 1,729. Based on the results of this study, it can be concluded that the results of DNA extraction carried out on salted fish processed food products show a value that is in the category of good DNA extraction results.
DNA detection of porcine species in processed meat samples (shredded meat) was carried out as an ... more DNA detection of porcine species in processed meat samples (shredded meat) was carried out as an effort to enrich the literature and reference alternative testing methods in the control of halal products circulating in Indonesia. The purpose of this study was to detect the DNA of porcine species in samples using real-time PCR. The research method used is a qualitative technique. This technique analyzes the CT value of the sample compared to the positive control by looking at the formation of the sigmoid curve if amplification occurs. The controls used consisted of negative control, positive control, other DNA control, and LOD control. The extraction technique used is the column centrifuge technique. The extraction results were then measured using a nano photometer to see the purity and concentration of the extracted DNA. Based on the research, it is known that the extraction results read on a nanophotometer at a wavelength of A260 / A280 show a concentration of 24,350 with a purity ...
HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY
The authentication test for spices made from turmeric using real-time. This study aimed to determ... more The authentication test for spices made from turmeric using real-time. This study aimed to determine whether the spice ingredients claimed to be made from turmeric actually contain turmeric, as asserted by the product. The sample set consisted of 10 types of spices based on turmeric, which were obtained from various supermarkets. The research employed real-time PCR analysis using the SYBR Green method as the chosen approach. Data analysis was performed based on Ct analysis and Tm analysis. The measurement of DNA purity and concentration resulted in a DNA concentration value of 56.205 and a DNA purity value of 2.302, measured at a wavelength of A260/A280. The real-time PCR analysis yielded Ct values ranging from 15.20 to 15.83 with an average of 15.50, and Tm values ranging from 78.70 to 89.00 with an average of 78.83. Based on these data, it can be concluded that the ready-to-use spice products, claimed to be made from genuine turmeric, indeed contain turmeric, as supported by the r...
Analysis of the purity and concentration of DNA isolation results was carried out using samples o... more Analysis of the purity and concentration of DNA isolation results was carried out using samples of dragonflies (Onychogomphus forcipatus) species of insects. This research is important because there are many kinds of research in the molecular field that require good DNA isolation techniques to support the success of the research. The purpose of this paper is to provide information about the quality of the isolated DNA seen using the purity and concentration parameters. The extraction method uses the centrifuge column method. The isolated data were analyzed statistically using the average test. The results of DNA isolation showed that the average concentration value was in the range of 60.10 – 69.95. The analysis of the purity of the isolated DNA read at the A260/A280 ratio was in the range of 1.817 – 1.929, while the purity analysis at the A260/A230 ratio was in the range of 0.760 – 0.822. Based on the results of the study, it can be concluded that the analysis of the purity and con...
HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY
Real-Time PCR is a molecular biology testing instrument used for various types of food product te... more Real-Time PCR is a molecular biology testing instrument used for various types of food product testing applications to monitor the quality of a product. In addition, the real-time PCR technique can also be used to test the detection of pathogenic bacteria in some foods and processed products. The purpose of this study was to detect Salmonella typhimurium using real-time PCR. This research can be a source of information and a reference for similar research. The test method used is the real-time PCR method with the green SYBR kit. Data analysis was carried out by looking at the results of interpreting Ct values and Tm values. Positive results are indicated by the formation of the Ct curve and the Tm curve. From the research conducted, it was found that the Ct value was in the range of 11.90 - 12.10 with an average of 12.02, while the Tm value was in the range of 85.10 - 85.20 with an average of 85.15. From the study results, it can be concluded that the use of real-time PCR for testin...
Analysis of the purity and concentration of isolated DNA is a way to see the quality of isolated ... more Analysis of the purity and concentration of isolated DNA is a way to see the quality of isolated DNA. This research was conducted as an initial stage to give. Information about research conducted in future research. The purpose of this research is to provide references and information regarding DNA isolation techniques in chondroitin samples so that they can be used in similar studies. The sample consisted of a sample of chondroitin which was weighed 50 mg and tested 10 times. The isolation method used in this study is the centrifuge column isolation method, while the purity and concentration analysis were analyzed using a nano photometer which was read at a wavelength of A260/A280. The value shown from the nano photometer was then analyzed statistically using the average test to determine the interpretation of the results from the data obtained. The results of DNA isolation obtained that the DNA concentration values tested were in the range of 39.10-54.70 with an average concentration value of 45.15. The value of the purity of the isolated DNA tested was in the range of 2.16-2.28 with an average purity value of 2.22. Based on the results of the DNA confirmation test of the isolation carried out using real-time PCR, it showed that the isolated sample was amplified at a value of Ct 36.43 while the positive control was amplified at Ct 32.49. Based on the research results, it was found that all samples tested showed good average values of DNA concentration and purity so that the results of the DNA isolation tested could be used as templates in real-time PCR analysis.
Analisis mutu DNA untuk melihat kualitas DNA hasil isolasi merupakan sebuah teknik analisis yang ... more Analisis mutu DNA untuk melihat kualitas DNA hasil isolasi merupakan sebuah teknik analisis yang dapat digunakan untuk mendukung pengujian bidang biologi molekuler yang menggunakan instrumen PCR atau real-time PCR. Tujuan dari penelitian ini adalah untuk menganalisis kualitas DNA hasil isolasi pada sampel produk pangan olahan ikan. Manfaat penelitian ini diharapkan dapat menjadi sumber informasi yang mendukung penelitian di bidang molekuler pada ruang lingkup pengawasan mutu dan autentifikasi pada produk pangan olahan ikan. Analisis DNA hasil isolasi dianalisis menggunakan NanoPhotometer untuk mengukur nilai konsentrasi dan kemurnian dengan panjang gelombang A260/A280. Data yang diperoleh dari hasil pengukuran kemudian diinterpretasikan menggunakan nilai uji rata-rata untuk menganalisis mutu DNA hasil isolasi. Data hasil penelitian menunjukan nilai konsentrasi DNA berada pada kisaran 14,5 – 17,8 dengan rata-rata nilai konsentrasi berada pada 16,1. Untuk nilai kemurnian DNA hasil is...
Muhammadiyah Journal of Nutrition and Food Science (MJNF)
Latar belakang: Parameter nilai kemurnian yang dianalisis dari panjang gelombang dengan rasio 260... more Latar belakang: Parameter nilai kemurnian yang dianalisis dari panjang gelombang dengan rasio 260/230 merupakan parameter validasi sekunder yang digunakan untuk melakukan analisis mutu DNA hasil isolasi pada produk nugget ayam. Tujuan: Tujuan dari dilakukannya penelitian ini adalah untuk memberi informasi tentang nilai analisis kemurnian DNA hasil isolasi yang dibaca pada rasio 260/230. Dengan diperolehnya informasi dari hasil penelitian ini diharapkan dapat memberi manfaat terhadap penelitian sejenis yang relevan. Metode: Metode yang digunakan dalam penelitian ini adalah metode isolasi DNA menggunakan teknik spin kolom, kemudian DNA hasil isolasi yang diperoleh dibaca menggunakan nano fotometer pada rasio 260/230. Hasil: Hasil pengukuran kemudian dihitung nilai rata-rata dan standar deviasinya. Berdasarkan hasil pengukuran DNA hasil isolasi diperoleh hasil kemurnian yang dibaca pada rasio A260/A230 berada pada kisaran 1,98 – 2,10, dengan nilai rata-rata berada pada nilai 2,043. S...
HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY
DNA isolation is one of the important steps in conducting PCR-based or real-time PCR-based molecu... more DNA isolation is one of the important steps in conducting PCR-based or real-time PCR-based molecular analysis. This study aims to test the quality of DNA isolation extracted from samples of processed food products from chicken meat (chicken nuggets). The novelty of this research lies in the DNA isolation technique modified from the manual kit which is used to produce good-quality DNA isolation results. The extraction method used was the modified extraction method and the standard extraction method of the DNeasy Mericon Food kit. Analysis of DNA quality was measured using a nanophotometer, where the analysis of DNA quality was analyzed based on the parameters of concentration values and purity values. Based on research data, it is known that the modified DNA extraction method produces a concentration value of DNA isolation with an average concentration value of 1295.1 ng/µL. Meanwhile, the value of the purity of the isolated DNA was 2.10 on average. while the standard DNA extraction ...
The myostatin (MSTN) gene provides instructions for making myostatin, which helps control the gro... more The myostatin (MSTN) gene provides instructions for making myostatin, which helps control the growth and development of tissue throughout the body and limits muscle growth. Myostatin has been studied in mice, cows, and other animals. However, the MSTN gene has not been studied in the nisi chicken. Further, the lack of information using the real-time polymerase chain reaction (PCR) to detect the MTSN gene is still to be explored. Accordingly, this study aimed to detect the MSTN gene in nisi chickens, one of the local-endemic Indonesian chickens, using real-time PCR. DNA of twenty-five male chickens was extracted using an automatic DNA extraction tool, and the MSTN gene was analyzed by real-time PCR Rotor-Gene 5 Plex. Ct and Tm values were indicators of the successful amplification of the MSTN gene. The results showed that the MSTN gene was detected by Ct 16.00 and Tm 80.00, respectively. The study novelty is based on the nisi chicken as the local-endemic chicken from Indonesia as the...
BiosciED: Journal of Biological Science and Education
Escherichia coli bacteria test on contaminated meat processed food products with several variatio... more Escherichia coli bacteria test on contaminated meat processed food products with several variations of positive control concentrations was carried out to provide additional information about determining the LOD value in the test method for the detection of Escherichia coli pathogenic bacteria. The purpose of this study was to detect and identify bacterial contamination of Escherichia coli ATCC 25922 in contaminated meat processed food products with variations in the concentration of positive control. The method used to identify is the enrichment method, using enrichment media to grow the suspected target bacterium Escherichia coli ATCC 25922 spiked in meat-processed food products, followed by isolation using selective media and ending with confirmation and affirmation tests. The samples used were 10 packages of processed meat food products. The samples were then spiked using various concentrations of positive control Escherichia coli 5, 10, 50, and 100 colonies/gr. The data from the...
Eruditio : Indonesia Journal of Food and Drug Safety
The presence of gelatin components can be identified through fat, protein and DNA. DNA can be obt... more The presence of gelatin components can be identified through fat, protein and DNA. DNA can be obtained through the DNA isolation process which is the process of separating DNA from other components of the cell. The DNA purification stage is the most important thing in DNA isolation because at this stage it can reduce to a minimum the number of contaminants in DNA isolates to determine the purity and concentration of the DNA isolate obtained. The purpose of this technique is to produce DNA isolates that are low inhibitors or are in the purity range of 1.7-2.1. The extraction method used in this study used the double wash technique, the technique was modified from the test stage following the manual kit used, it's just that the technique used was modified at the washing stage with each washing done twice in the washing section. first and second washing so that a total of four items of washing were carried out. The results of the DNA isolates were then analyzed for the purity and c...
Detection of Salmonella typhimurium ATCC 14028 bacteria in bakery product samples by real-time PC... more Detection of Salmonella typhimurium ATCC 14028 bacteria in bakery product samples by real-time PCR using boiling isolation technique. The basis of this research is to have an impact on economic value in carrying out the confirmation test for Salmonella typhimurium ATCC 14028, where testing is carried out conventionally will require large costs, so it is necessary to innovate in terms of modifying the testing phase so that it is more effective and efficient. The purpose of this study was to see whether the boiling isolation technique could be used for the detection test for Salmonella typhimurium ATCC 14028 on bacterial product samples. The sample in this study consisted of 15 types of bacterial product samples spiked with Salmonella typhimurium ATCC 14028 cultures that had been cultured into phase 2 working cultures. The method used in this study was qPCR analysis using the SYBR Green method. The results of real-time PCR analysis obtained Ct values in the range 7.55 - 8.91 with an a...
Species DNA detection using genetic markers of the PGR gene on chicken nuggets was carried out to... more Species DNA detection using genetic markers of the PGR gene on chicken nuggets was carried out to see whether these genetic markers could be used to test species DNA detection in chicken nuggets food products. The test method used in this study is a real-time PCR test method using the SYBR green technique where the test results can be either Ct or Tm values which indicate amplification or detection. The results of DNA isolation showed that the concentration and purity of the isolated DNA were in the range of 35.10 ng/µL – 36.10ng/µL with an average of 35,488 ng/µL. As for the purity value measured at the A260/A280 wavelength, the results were obtained with a purity range between 2,110 – 2,250 with an average of 2,165. For the results of real-time PCR amplification, the Ct value of the chest sample was at 24.50, the Ct LOD value was at 21.20 and the Ct value of the positive control was 27.10. For the Tm value of the busty sample at 81.10, the Tm LOD value at 81.20 and the positive ...
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