Papers by Alena Gajdosova
Acta horticulturae, Dec 1, 2010
Fifty-year-old specimens of Betula pendula 'Dalecarlica', 'Fastigiata', 'Purpurea', 'Youngii', an... more Fifty-year-old specimens of Betula pendula 'Dalecarlica', 'Fastigiata', 'Purpurea', 'Youngii', and var. typica were used as donor plants. For callus induction, leaves obtained from in vitro were used as initial explants. The effects of the cultivar types and different media (MS, WPM and S) supplemented with zeatin (2, 5, and 10 mg L -1 ) or BA (0.5, 0.8, and 1.0 mg L -1 ) on adventitious bud formation from callus segments were studied. The best regeneration potential was observed in 'Youngii' and the purest in 'dalecarlica'. The best results were obtained on medium S containing 5 and 10 mg L -1 zeatin. The effect of IBA (0.3 and 0.5 mg L -1 ), NAA (0.3 and 0.5 mg L -1 ) and their combinations on the rooting of adventitious shoots was investigated. The highest percentage of rooting (100%) and the best root system was achieved on half-strength MS medium in combination with 0.3 mg L -1 IBA and 0.3 mg L -1 NAA. The high air humidity and the type of substrate were important factors for successful acclimatization. Maximum survival of plants (from 98.3 ± 1.7% up to 100.0 ± 0.0%) was obtained by opening the test tubes in the cultivation room for a period of 6 days, ensuring high air humidity for 14 days, and utilizing peat. The genotypes of the donor and in vitro propagated plants were analyzed by seven isozyme systems (GOT, IDH, LAP, MDH, PGI, PGM, and 6-PGD). Changes of the isozyme fractions of in vitro-propagated plants in comparison with the donor plants were not found.
Book of Abstracts, 2022
Targeting sonic hedgehog pathway in combination with proton radiation or gamma irradiation decrea... more Targeting sonic hedgehog pathway in combination with proton radiation or gamma irradiation decreases viability of glioma cell lines .
Genetika, 2015
The introduction of foreign DNA into the plant genome by Agrobacterium tumefaciens is a promising... more The introduction of foreign DNA into the plant genome by Agrobacterium tumefaciens is a promising technique of targeted gene transfer which depends on good working regeneration system. The aim of the work was to elaborate the system for efficient adventitious organogenesis and transgenic plant regeneration in Rubus fruticosus L. using explants from mature plants. Regeneration of putative transgenic shoots took place from flag explants cultivated vertically on MS medium with 1 mg l-1 TDZ and 0.02 mg l-1 IBA followed by transfer on MS medium with 1 mg l-1 BAP, 0.02 mg l-1 IBA and 0.1 mg l-1 GA 3 supplemented with 10-15 mg l-1 hygromycin after transformation by A. tumefaciens strain LBA 4404 carrying plasmid pCambia 1304. Four putative transgenic plants of cv. 'Čačanska Bestrna' were rooted and acclimatized.
Springer eBooks, 2007
Oaks are important broad-leaved hardwood species of forest ecosystems, valuable from economical a... more Oaks are important broad-leaved hardwood species of forest ecosystems, valuable from economical and ecological point of view. The preferred means of oak propagation is from seeds, what favours broadening of genetic diversity of these species. However, the possibilities of generative reproduction are limited by long life span necessary for achievement of physiological maturity, by irregular occurrence of mast years, by
… Vestis (Latvia); Latvian …, 2009
... Mária Gabriela Ostrolucká1, Alena Gajdoová1, Emilia Ondruková2 and Gabriela Libiaková1 1 In... more ... Mária Gabriela Ostrolucká1, Alena Gajdoová1, Emilia Ondruková2 and Gabriela Libiaková1 1 Institute of Plant Genetics and Biotechnology SAS, Akademická 2, POBox 39A, 950 07 Nitra, Slovak Republic; e-mail: gabriela ... (Chandler and Draper, 1986; Rowland and Ogden ...
Book of Abstracts, 2022
Targeting sonic hedgehog pathway in combination with proton radiation or gamma irradiation decrea... more Targeting sonic hedgehog pathway in combination with proton radiation or gamma irradiation decreases viability of glioma cell lines .
Acta horticulturae, 2012
Murashige and Skoog medium (MS) and modified Anderson's Rhododendron medium (mAN) were compared f... more Murashige and Skoog medium (MS) and modified Anderson's Rhododendron medium (mAN) were compared for in vitro shoot multiplication of three highbush blueberries 'Berkeley', 'Bluecrop' and 'Goldtraube'. All media contained 0.5 mg/L zeatin applied either alone or combined with 0.1, 1 and 5 mg/L IBA. In vitro rooting was induced using mAN medium supplemented with 0.8 mg/L IBA and 4 g/L activated charcoal. The results obtained showed that mAN medium is more suitable for in vitro multiplication of the selected highbush blueberry cultivars than MS medium. Low concentration of IBA (≤1 mg/L) added in zeatin-supplemented mAN medium increases shoot multiplication efficiency of highbush blueberries in vitro and can be recommended for large-scale propagation of high quality plants. MS medium induced partial or full necrosis of stems and leaves, which was more pronounced on media containing zeatin combined with increasing concentration of IBA. Rooting capacity of shoots varied widely among the tested blueberry cultivars. The highest rooting and acclimatisation rates were achieved in 'Goldtraube' (82.8 and 91.8% respectively), and the lowest (10 and 66.7% respectively) were in 'Berkeley'.
Plants, Jun 6, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of Berry Research, Aug 28, 2019
BACKGROUND: Amelanchier alnifolia is an attractive small fruit difficult to propagate by traditio... more BACKGROUND: Amelanchier alnifolia is an attractive small fruit difficult to propagate by traditional methods. Moreover, this species can be susceptible to dormancy after transplanting to the soil. OBJECTIVE: The aim of this work was to evaluate factors that contribute to effective in vitro rooting and acclimatization of micropropagated shoots of Amelanchier alnifolia. METHODS: Different auxins, media concentrations, and sprays containing plant growth regulators were tested. The experimental data were treated by analysis of variance. RESULTS: 1-naphthalene acetic acid (NAA) was superior for rooting compared to indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). Cultivation of shoots on Murashige & Skoog (MS) medium with a half-strength concentration (1 / 2 MS) led to higher rooting frequencies than on full-strength MS medium. Addition of 1.5 mg l-1 spermidine to cultivation medium did not significantly improve rooting. Gibberellic acid (GA 4 + 7) alone or in combination with 6-benzylaminopurine (BAP) did not effectively break post-rooting dormancy. CONCLUSION: The greatest number of actively growing plants was recorded after rooting the shoots on 1 / 2 MS medium with 1 mg l-1 NAA followed by a spray treatment with 1 mg l-1 BAP. These results are directly applicable for improving rooting efficiency and acclimatization of micropropagated Amelanchier spp. plantlets.
CRC Press eBooks, Jan 6, 2007
Chemicke Listy, Aug 15, 2011
Investigation of Protein Profile Changes in Amaranth Seeds after Radiation Mutagenesis The main f... more Investigation of Protein Profile Changes in Amaranth Seeds after Radiation Mutagenesis The main focus of the study was to investigate the changes in protein content due to radiation mutagenesis by comparing the protein profile of amaranth mutant lines with that of untreated controls. We have found several promising lines with high coefficients of nutrition quality. Considering overall nutrition values, the line C82/1 is the most promising genotype, which could be possibly used in a future breeding programme. The protein composition of amaranth seeds can be changed and their nutrition values significantly influenced through radiation mutagenesis.
Agronomy, Dec 16, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of Central European Agriculture, 2016
The aim of this work was to develop protocols for micropropagation of selected Rubus cultivars-'T... more The aim of this work was to develop protocols for micropropagation of selected Rubus cultivars-'Tulameen' and 'Black Jewel' (Rubus idaeus L.), 'Black Satin' (Rubus fruticosus L.) and 'Tayberry' (Rubus fruticosus x Rubus idaeus). Nodal segments carrying dormant buds were used for in vitro culture establishment. For shoot initiation, MS medium containing 8 g*L-1 phytoagar, 30 g*L-1 sucrose, 1 mg*L-1 6-benzylaminopurine (BAP), 0.2 mg*L-1 indole-3-butyric acid (IBA) and 150 mg*L-1 cefotaxime was used. The shoot proliferation ability of each cultivar was evaluated by multiplication coefficient (number of shoots formed per explant) during 4 subcultures. Statistical analyses confirmed the significantly higher shoot multiplication ability in cvs. 'Black Satin' and 'Tulameen' in comparison with cvs. 'Tayberry' and 'Black Jewel', as well as significant differences in shoot proliferation between subcultures (1 st and 2 nd versus 3 rd and 4 th). Testing of influence of different BAP concentrations on shoot formation showed that BAP 1-2 mg*L-1 is the best for shoot proliferation with the higher multiplication rate obtained in cv. 'Black Satin' in comparison with 'Black Jewel'. Statistical evaluation showed that genotype and BAP concentration significantly influenced the ability of shoot proliferation in Rubus cultivars.
Turkish Journal of Agriculture and Forestry, 2016
Introduction Plants, and particularly the horticulture section, are used by people for food, eith... more Introduction Plants, and particularly the horticulture section, are used by people for food, either as edible products or for culinary ingredients, and for medicinal use or ornamental and aesthetic purposes. They are genetically a very diverse group and play a major role in modern society end economy. Fruits and vegetables are important components of traditional food, but are also central to healthy diets of modern urban populations (
Antioxidants, 2021
Amaranth species represent a diverse group of plants. Many of them are a rich source of secondary... more Amaranth species represent a diverse group of plants. Many of them are a rich source of secondary metabolites with many positive biological effects. Total phenolic, total flavonoid and rutin content, antioxidant activity against superoxide and hydroxyl radicals, FRAP (Ferric-reducing ability of plasma) assay and DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging assay were determined in ethanol extracts of dried leaves of the new Slovak amaranth varieties ‘Pribina’ and ‘Zobor’. The amount of total phenolic substances (‘Pribina’ GAE 38.3 mg.g−1 DM and ‘Zobor’ GAE 26.1 mg.g−1 DM), content of total flavonoids (‘Pribina’ QE 26.5 mg.g−1 DM and ‘Zobor’ QE 20.3 mg.g−1 DM) and rutin (‘Pribina’ 50.8 mg.g−1 DM and ‘Zobor’ 15.2 mg.g−1 DM) were higher in the variety ‘Pribina’, compared to the variety ‘Zobor’. A statistically higher antioxidant activity against superoxide radical (1.63%·mg−1g−1 DM), hydroxyl radical (3.20%.mg−1g−1 DM), FRAP assay (292.80 µmol.L−1·mg−1.g−1 DM) and DPPH (54.2...
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Papers by Alena Gajdosova