International Journal of Microbiology and Mycology (IJMM), Sep 10, 2013
Antagonist activities of different Trichoderma strains were evaluated in vitro against Colletotri... more Antagonist activities of different Trichoderma strains were evaluated in vitro against Colletotrichum capsici, a causal agent of anthracnose fruit rot of chili. Dual culture test showed that Trichoderma strains effectively inhibited mycelia growth of the pathogen. T. harzianum IMI-392433 showed the highest inhibition (81.96 %) and mycelial overgrowth(78.98%). Also, metabolites having 80% concentration extracted from 30-day-old T. harzianum IMI-392433 revealed the highest PIRG (percentage inhibition of radial growth) value of 85.16 and 87.18% by using normal poison and modified bilayer poison agar technique, respectively. Further, metabolites extracted from 30-day-old T. harzianum IMI-392433 at a concentration of 2000 mgL-1completely inhibited spore germination and germ tube elongation of the pathogen. Also, severity of anthracnose was significantly decreased as compared with the control (2% methanol) when chili fruits were soaked in 2000 mgL-1of 30-day-old metabolites from T. harzia...
Machine Learning has a significant impact on different aspects of science and technology includin... more Machine Learning has a significant impact on different aspects of science and technology including that of medical researches and life sciences. Diabetes Mellitus, more commonly known as diabetes, is a chronic disease that involves abnormally high levels of glucose sugar in blood cells and the usage of insulin in the human body. This article has focused on analyzing diabetes patients as well as detection of diabetes using different Machine Learning techniques to build up a model with a few dependencies based on the PIMA dataset. The model has been tested on an unseen portion of PIMA and also on the dataset collected from Kurmitola General Hospital, Dhaka, Bangladesh. The research is conducted to demonstrate the performance of several classifiers trained on a particular country’s diabetes dataset and tested on patients from a different country. We have evaluated decision tree, K-nearest neighbor, random forest, and Naïve Bayes in this research and the results show that both random fo...
Trichoderma fungus species are highly populations of fungi in world that they can colonize roots ... more Trichoderma fungus species are highly populations of fungi in world that they can colonize roots as plant symbiosis. Various types of Trichoderma are free-living fungi that are, generally, saprophytic on the remains of soil. In addition to its role in biological control, this fungus increases plant yield and growth. So far, many studies have been conducted to examine the ability of this agent to reduce biological tensions and biological control of plant pathogens. Thus, this study was conducted to isolate and identify species of Trichoderma fungus from rapeseed fields in Golestan and Qazvin province from Iran, and also to study isolated species on germination percentage and growth parameters of rapeseed seedlings in a randomized complete block design with three replications in vitro. Based on the results, three species of T. harzianum, T. virens, and T. atroviride were identified, which the isolate of T. harzianum Ta19 showed a significant effect on the control group and other treat...
Proceedings of the 2016 SIAM International Conference on Data Mining, 2016
Ensembles of graphs arise in several natural applications. Many techniques exist to compute frequ... more Ensembles of graphs arise in several natural applications. Many techniques exist to compute frequent, dense subgraphs in these ensembles. In contrast, in this paper, we propose to discover maximally variable regions of the graphs, i.e., sets of nodes that induce very different subgraphs across the ensemble. We first develop two intuitive and novel definitions of such node sets, which we then show can be efficiently enumerated using a level-wise algorithm. Finally, using extensive experiments on multiple real datasets, we show how these sets capture the main structural variations of the given set of networks and also provide us with interesting and relevant insights about these datasets.
Native populations of bacteria were isolated from different habitats like soil, drain and garbage... more Native populations of bacteria were isolated from different habitats like soil, drain and garbage and the diversity were characterized. In all habitats total c.f.u's of bacteria were varied significantly (p=0.05) and were almost positively correlated with the characteristics of the habitat. On the basis of cultural and microscopic characteristics and results of biochemical test the isolated strains were identified as Bacillus sp. (SMH/2), Bacillus sp. (GLL/5), Cellulomonas sp., Micrococcus sp. and Pseudomonas sp. In this study BCDA medium was suitable for massive growth of Micrococcus sp. and Pseudomonas sp. and NA medium was suitable for Bacillus sp and Cellulomonas sp. The optimum ranges of pH and temperature were 7.25-7.55 and 25-35 <C, respectively. In Micrococcus sp. and Pseudomonas sp suitable incubation period was 24 hours but in Bacillus sp. (SMH/2), Bacillus sp. (GLL/5) and Cellulomonas sp. it was 36 hours. These selected bacteria were used for bioconversion of municipal solid waste to select the most effective strain. Bacterial suspension was found more effective then culture discs. The highest weight loss was observed 26.54% when bacterial suspension was used and it was 15.34% higher then control.
T. harzianum IMI-392434 were evaluated for antagonist potential against chili's fruit rot pathoge... more T. harzianum IMI-392434 were evaluated for antagonist potential against chili's fruit rot pathogen (A. tenuis) using dual culture, poison agar and direct assay methods. Two dual culture methods were applied and the highest percent inhibition of radial growth (PIRG) values occurred at 60.81±0.85 and 77.59±2.14% with T. harzianum IMI-392432 for first and second method, respectively. The minimum colony overgrowth time was recorded in T. harzianum IMI-392432 and maximum was exhibited in T. pseudokoningii IMI-392431. The PIRG values of Trichoderma strains against A. tenuis were significantly (P<0.05) varied at different concentrations of metabolites of different days. The highest PIRG values (84.64±1.25%) were achieved at 80% concentration on the 4 th day, with 30-day-old metabolites of T. harzianum IMI-392432 in normal poison agar. But in modified bilayer poison agar, the highest PIRG values (85.9±0.44%) were recorded at the same concentration and the same 30-day-old-metabolites of T. harzianum IMI-392432. In direct assay method, maximum percentage of inhibition of mycelial growth weight (PIGW) was achieved at the same concentration and the same days old metabolites of T. harzianum IMI-392432. Present study showed that the Trichoderma has a good antagonistic effect on mycelial growth of A. tenuis, and T. harzianum IMI-392432 has the highest potential to be applied in order to control fruit rot pathogen of chili.
To investigate the effects of bio compost, cow dung compost and NPK fertilizers on growth, yield ... more To investigate the effects of bio compost, cow dung compost and NPK fertilizers on growth, yield and yield components of chili the experiment was conducted randomized block design with three replications at Botanical Garden of Rajshahi University Campus, Bangladesh during August 2008 to February 2009. There were 15 treatments viz. T1 = bio compost (3 kg/pot) + NPK, T2 = bio compost (2 kg/pot) + NPK, T3 = bio compost (1.5 kg/pot) + NPK, T4 = bio compost (3 kg/pot), T5 = bio compost (2 kg/pot), T6 = bio compost (1.5 kg/pot), T7 = cow dung compost 3 kg/pot + NPK, T8 = cow dung compost (2 kg/pot) + NPK, T9 = cow dung compost (1.5 kg/pot) + NPK, T10 = cow dung compost (3 kg/pot), T11 = cow dung compost (2 kg/pot), T12 = cow dung compost (1.5 kg/pot), T13= NPK, T14= bacterial suspension, T15= control (only soil). Bio compost and NPK significantly (p=0.05) influenced the growth and yield of chili. The treatment bio compost (3kg/pot) +NPK (T1) produced the highest germination (%), vigour index, growth and yield of chili and the lowest yield and yield contributing parameters were recorded in control (T15). The correlation matrix showed that yield per plant of chili had significant and positive correlation with plant height (r = 0.929**), leaf number (r = 0.808**), number of primary branch (r = 0.918**), secondary branch (r = 0.985**), root number (r = 0.953**), root length (r = 0.947**), total number of flower at maximum flowering time(r = 0.981**), total number of fruit (r = 0.966**), fruit length (r = 0.917**), fresh fruit weight (r = 0.990**), dry fruit weight (r = 0.800**), number of seed/ fruit (r = 0.861**) and hundred seed weight (r = 0.954**) and yield was significant and negative correlation (r =-0.906**) with number of days required for first flower initiation. The results suggest that inorganic fertilizers (NPK) with bio compost (3kg/pot) is suitable for better production of chili that may increase soil fertility and this integrated approach could be contributed to improve crop production.
In this study, dual culture, poison agar, and direct methods were used to assess the ability of T... more In this study, dual culture, poison agar, and direct methods were used to assess the ability of Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433, and T. harzianum IMI-392434 to control Ceratocystis paradoxa, which causes the pineapple disease of sugarcane. The highest percentage inhibition of radial growth (PIRG) values were observed with T. harzianum IMI-392432 using two dual culture methods, 63.80% in Method I and 80.82% in Method II. The minimum colony overgrowth time was observed with T. harzianum IMI-392432 and the maximum was observed with T. pseudokoningii IMI-392431. Different concentrations of different day-old metabolites of Trichoderma isolates were tested against mycelial growth of C. paradoxa. The highest PIRG (84.685%) exhibited at 80% concentration of 30-day-old metabolites of T. harzianum IMI-392432 using the modified bilayer poison agar method. In the direct assay method the maximum mycelial growth weight ...
Five strains of Trichoderma were used to select the most effective strain for bioconversion of Mu... more Five strains of Trichoderma were used to select the most effective strain for bioconversion of Municipal solid waste. Culture pellet was found more effective than spore suspension. The highest weight loss was 31.80 %, when culture pellet (6 pieces) was used and it was 12% higher than control. Autoclaved solid waste was good for degradation than non-autoclaved. Among the studied strains, T. harzianum (IMI-392432) was found most effective. Using combination of Trichoderma showed 10% better result than single strain used.
2008 International Conference on Electrical and Computer Engineering, 2008
... Sharif Uddin, Rahat Khan, ABM Tariqul Islam, SM Rafizul Haque, A New Approach in Computer Re... more ... Sharif Uddin, Rahat Khan, ABM Tariqul Islam, SM Rafizul Haque, A New Approach in Computer Representation of Bangla Words and Bangla Sorting Algorithm, in Proceeding of National Conference on Computer Processing of Bangla, Dhaka, Bangladesh, 2005. ...
2010 13th International Conference on Computer and Information Technology (ICCIT), 2010
Given a graph G = (V, E) and two positive integers k and ∆, the ∆-closest phylogenetic k-th root ... more Given a graph G = (V, E) and two positive integers k and ∆, the ∆-closest phylogenetic k-th root problem (∆CP R k) is to find a (phylogenetic) tree T such that the degree of each internal node in T is at least three and at most ∆, the external nodes of T are exactly the elements of V , and the number of "kdisagreements" is minimized. In this paper we give an approximation algorithm for ∆CP R2 for any fixed ∆ > 3. The expected ratio of our algorithm is 3, if there is no vertex with degree more than ∆ − 2, and ∆ + 2, otherwise, which improves the best known previous ratio of 8 for ∆ ≤ 5.
T. virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392... more T. virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433 and T. harzianum IMI-392434 were evaluated for their potentiality on seed germination and seedling parameters in chili both laboratory and field conditions. Chili seeds were coated with spore suspension of each test strains of Thichoderma supplemented with 2 % of starch (w/v) as an adhesive. Seed germination percentages and the vigour index were significantly affected by the application of different strains of Trichoderma. Among the five Trichoderma strains, T. harzianum IMI-3924332 gave the highest germination percentage followed by T. harzianum IMI-3924333, T. harzianum IMI-3924334, T. virens IMI-392430 and T. pseudokoningii IMI-392431 treatment both in laboratory and field conditions, respectively while control decrease these value. Chili seeds also gave the highest vigour index values with T. harzianum IMI-3924332 which confirmed to better germination. Seed treatment with T. harz...
International Journal of Microbiology and Mycology (IJMM), Sep 10, 2013
Antagonist activities of different Trichoderma strains were evaluated in vitro against Colletotri... more Antagonist activities of different Trichoderma strains were evaluated in vitro against Colletotrichum capsici, a causal agent of anthracnose fruit rot of chili. Dual culture test showed that Trichoderma strains effectively inhibited mycelia growth of the pathogen. T. harzianum IMI-392433 showed the highest inhibition (81.96 %) and mycelial overgrowth(78.98%). Also, metabolites having 80% concentration extracted from 30-day-old T. harzianum IMI-392433 revealed the highest PIRG (percentage inhibition of radial growth) value of 85.16 and 87.18% by using normal poison and modified bilayer poison agar technique, respectively. Further, metabolites extracted from 30-day-old T. harzianum IMI-392433 at a concentration of 2000 mgL-1completely inhibited spore germination and germ tube elongation of the pathogen. Also, severity of anthracnose was significantly decreased as compared with the control (2% methanol) when chili fruits were soaked in 2000 mgL-1of 30-day-old metabolites from T. harzia...
Machine Learning has a significant impact on different aspects of science and technology includin... more Machine Learning has a significant impact on different aspects of science and technology including that of medical researches and life sciences. Diabetes Mellitus, more commonly known as diabetes, is a chronic disease that involves abnormally high levels of glucose sugar in blood cells and the usage of insulin in the human body. This article has focused on analyzing diabetes patients as well as detection of diabetes using different Machine Learning techniques to build up a model with a few dependencies based on the PIMA dataset. The model has been tested on an unseen portion of PIMA and also on the dataset collected from Kurmitola General Hospital, Dhaka, Bangladesh. The research is conducted to demonstrate the performance of several classifiers trained on a particular country’s diabetes dataset and tested on patients from a different country. We have evaluated decision tree, K-nearest neighbor, random forest, and Naïve Bayes in this research and the results show that both random fo...
Trichoderma fungus species are highly populations of fungi in world that they can colonize roots ... more Trichoderma fungus species are highly populations of fungi in world that they can colonize roots as plant symbiosis. Various types of Trichoderma are free-living fungi that are, generally, saprophytic on the remains of soil. In addition to its role in biological control, this fungus increases plant yield and growth. So far, many studies have been conducted to examine the ability of this agent to reduce biological tensions and biological control of plant pathogens. Thus, this study was conducted to isolate and identify species of Trichoderma fungus from rapeseed fields in Golestan and Qazvin province from Iran, and also to study isolated species on germination percentage and growth parameters of rapeseed seedlings in a randomized complete block design with three replications in vitro. Based on the results, three species of T. harzianum, T. virens, and T. atroviride were identified, which the isolate of T. harzianum Ta19 showed a significant effect on the control group and other treat...
Proceedings of the 2016 SIAM International Conference on Data Mining, 2016
Ensembles of graphs arise in several natural applications. Many techniques exist to compute frequ... more Ensembles of graphs arise in several natural applications. Many techniques exist to compute frequent, dense subgraphs in these ensembles. In contrast, in this paper, we propose to discover maximally variable regions of the graphs, i.e., sets of nodes that induce very different subgraphs across the ensemble. We first develop two intuitive and novel definitions of such node sets, which we then show can be efficiently enumerated using a level-wise algorithm. Finally, using extensive experiments on multiple real datasets, we show how these sets capture the main structural variations of the given set of networks and also provide us with interesting and relevant insights about these datasets.
Native populations of bacteria were isolated from different habitats like soil, drain and garbage... more Native populations of bacteria were isolated from different habitats like soil, drain and garbage and the diversity were characterized. In all habitats total c.f.u's of bacteria were varied significantly (p=0.05) and were almost positively correlated with the characteristics of the habitat. On the basis of cultural and microscopic characteristics and results of biochemical test the isolated strains were identified as Bacillus sp. (SMH/2), Bacillus sp. (GLL/5), Cellulomonas sp., Micrococcus sp. and Pseudomonas sp. In this study BCDA medium was suitable for massive growth of Micrococcus sp. and Pseudomonas sp. and NA medium was suitable for Bacillus sp and Cellulomonas sp. The optimum ranges of pH and temperature were 7.25-7.55 and 25-35 <C, respectively. In Micrococcus sp. and Pseudomonas sp suitable incubation period was 24 hours but in Bacillus sp. (SMH/2), Bacillus sp. (GLL/5) and Cellulomonas sp. it was 36 hours. These selected bacteria were used for bioconversion of municipal solid waste to select the most effective strain. Bacterial suspension was found more effective then culture discs. The highest weight loss was observed 26.54% when bacterial suspension was used and it was 15.34% higher then control.
T. harzianum IMI-392434 were evaluated for antagonist potential against chili's fruit rot pathoge... more T. harzianum IMI-392434 were evaluated for antagonist potential against chili's fruit rot pathogen (A. tenuis) using dual culture, poison agar and direct assay methods. Two dual culture methods were applied and the highest percent inhibition of radial growth (PIRG) values occurred at 60.81±0.85 and 77.59±2.14% with T. harzianum IMI-392432 for first and second method, respectively. The minimum colony overgrowth time was recorded in T. harzianum IMI-392432 and maximum was exhibited in T. pseudokoningii IMI-392431. The PIRG values of Trichoderma strains against A. tenuis were significantly (P<0.05) varied at different concentrations of metabolites of different days. The highest PIRG values (84.64±1.25%) were achieved at 80% concentration on the 4 th day, with 30-day-old metabolites of T. harzianum IMI-392432 in normal poison agar. But in modified bilayer poison agar, the highest PIRG values (85.9±0.44%) were recorded at the same concentration and the same 30-day-old-metabolites of T. harzianum IMI-392432. In direct assay method, maximum percentage of inhibition of mycelial growth weight (PIGW) was achieved at the same concentration and the same days old metabolites of T. harzianum IMI-392432. Present study showed that the Trichoderma has a good antagonistic effect on mycelial growth of A. tenuis, and T. harzianum IMI-392432 has the highest potential to be applied in order to control fruit rot pathogen of chili.
To investigate the effects of bio compost, cow dung compost and NPK fertilizers on growth, yield ... more To investigate the effects of bio compost, cow dung compost and NPK fertilizers on growth, yield and yield components of chili the experiment was conducted randomized block design with three replications at Botanical Garden of Rajshahi University Campus, Bangladesh during August 2008 to February 2009. There were 15 treatments viz. T1 = bio compost (3 kg/pot) + NPK, T2 = bio compost (2 kg/pot) + NPK, T3 = bio compost (1.5 kg/pot) + NPK, T4 = bio compost (3 kg/pot), T5 = bio compost (2 kg/pot), T6 = bio compost (1.5 kg/pot), T7 = cow dung compost 3 kg/pot + NPK, T8 = cow dung compost (2 kg/pot) + NPK, T9 = cow dung compost (1.5 kg/pot) + NPK, T10 = cow dung compost (3 kg/pot), T11 = cow dung compost (2 kg/pot), T12 = cow dung compost (1.5 kg/pot), T13= NPK, T14= bacterial suspension, T15= control (only soil). Bio compost and NPK significantly (p=0.05) influenced the growth and yield of chili. The treatment bio compost (3kg/pot) +NPK (T1) produced the highest germination (%), vigour index, growth and yield of chili and the lowest yield and yield contributing parameters were recorded in control (T15). The correlation matrix showed that yield per plant of chili had significant and positive correlation with plant height (r = 0.929**), leaf number (r = 0.808**), number of primary branch (r = 0.918**), secondary branch (r = 0.985**), root number (r = 0.953**), root length (r = 0.947**), total number of flower at maximum flowering time(r = 0.981**), total number of fruit (r = 0.966**), fruit length (r = 0.917**), fresh fruit weight (r = 0.990**), dry fruit weight (r = 0.800**), number of seed/ fruit (r = 0.861**) and hundred seed weight (r = 0.954**) and yield was significant and negative correlation (r =-0.906**) with number of days required for first flower initiation. The results suggest that inorganic fertilizers (NPK) with bio compost (3kg/pot) is suitable for better production of chili that may increase soil fertility and this integrated approach could be contributed to improve crop production.
In this study, dual culture, poison agar, and direct methods were used to assess the ability of T... more In this study, dual culture, poison agar, and direct methods were used to assess the ability of Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433, and T. harzianum IMI-392434 to control Ceratocystis paradoxa, which causes the pineapple disease of sugarcane. The highest percentage inhibition of radial growth (PIRG) values were observed with T. harzianum IMI-392432 using two dual culture methods, 63.80% in Method I and 80.82% in Method II. The minimum colony overgrowth time was observed with T. harzianum IMI-392432 and the maximum was observed with T. pseudokoningii IMI-392431. Different concentrations of different day-old metabolites of Trichoderma isolates were tested against mycelial growth of C. paradoxa. The highest PIRG (84.685%) exhibited at 80% concentration of 30-day-old metabolites of T. harzianum IMI-392432 using the modified bilayer poison agar method. In the direct assay method the maximum mycelial growth weight ...
Five strains of Trichoderma were used to select the most effective strain for bioconversion of Mu... more Five strains of Trichoderma were used to select the most effective strain for bioconversion of Municipal solid waste. Culture pellet was found more effective than spore suspension. The highest weight loss was 31.80 %, when culture pellet (6 pieces) was used and it was 12% higher than control. Autoclaved solid waste was good for degradation than non-autoclaved. Among the studied strains, T. harzianum (IMI-392432) was found most effective. Using combination of Trichoderma showed 10% better result than single strain used.
2008 International Conference on Electrical and Computer Engineering, 2008
... Sharif Uddin, Rahat Khan, ABM Tariqul Islam, SM Rafizul Haque, A New Approach in Computer Re... more ... Sharif Uddin, Rahat Khan, ABM Tariqul Islam, SM Rafizul Haque, A New Approach in Computer Representation of Bangla Words and Bangla Sorting Algorithm, in Proceeding of National Conference on Computer Processing of Bangla, Dhaka, Bangladesh, 2005. ...
2010 13th International Conference on Computer and Information Technology (ICCIT), 2010
Given a graph G = (V, E) and two positive integers k and ∆, the ∆-closest phylogenetic k-th root ... more Given a graph G = (V, E) and two positive integers k and ∆, the ∆-closest phylogenetic k-th root problem (∆CP R k) is to find a (phylogenetic) tree T such that the degree of each internal node in T is at least three and at most ∆, the external nodes of T are exactly the elements of V , and the number of "kdisagreements" is minimized. In this paper we give an approximation algorithm for ∆CP R2 for any fixed ∆ > 3. The expected ratio of our algorithm is 3, if there is no vertex with degree more than ∆ − 2, and ∆ + 2, otherwise, which improves the best known previous ratio of 8 for ∆ ≤ 5.
T. virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392... more T. virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433 and T. harzianum IMI-392434 were evaluated for their potentiality on seed germination and seedling parameters in chili both laboratory and field conditions. Chili seeds were coated with spore suspension of each test strains of Thichoderma supplemented with 2 % of starch (w/v) as an adhesive. Seed germination percentages and the vigour index were significantly affected by the application of different strains of Trichoderma. Among the five Trichoderma strains, T. harzianum IMI-3924332 gave the highest germination percentage followed by T. harzianum IMI-3924333, T. harzianum IMI-3924334, T. virens IMI-392430 and T. pseudokoningii IMI-392431 treatment both in laboratory and field conditions, respectively while control decrease these value. Chili seeds also gave the highest vigour index values with T. harzianum IMI-3924332 which confirmed to better germination. Seed treatment with T. harz...
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Papers by Ahsanur Rahman