Tropical animal health and production, Jun 1, 2024
cattle, pig, horses and small ruminants) as well as poultry where the economic importance is undo... more cattle, pig, horses and small ruminants) as well as poultry where the economic importance is undoubted (Almeida et al. 2021; Almeida et al. 2017; Ferlizza et al. 2015; Ribeiro et al. 2020). A main advantage of proteomics is the provision of a global view of protein expression patterns in an organism. This is critical for identifying new biomarkers, drug targets, and pathways involved in different biological processes (Petricoin and Liotta 2003). The tropics and the subtropics, being regions facing inherent local challenges, are particularly important in the framework of animal production, given the fact that the vast majority of the human population inhabits these regions. Over the last two decades, mass spectrometry (MS)based techniques have become prominent and a preferred approach for accurately and comprehensively identifying and quantifying proteins in biological samples (Schubert et al. 2017). These methods have played a pivotal role in advancing our comprehension of cellular signalling networks, shedding light on the intricate dynamics of protein-protein interactions across various cellular states, and enhancing our diagnostic capabilities and molecular insights into the mechanisms underlying diseases. Distinguishing between high-quality and low-quality proteomics Mounir Adnane
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Background and Aim: Foot-and-mouth disease (FMD), a major transboundary animal ailment in Algeria... more Background and Aim: Foot-and-mouth disease (FMD), a major transboundary animal ailment in Algeria, is a serious economic burden on the livestock sector. This study aimed to investigate the spatiotemporal distribution of FMD in Algeria and identifies the factors contributing to this phenomenon. Materials and Methods: Data on FMD cases occurring in Algeria from 2014 to 2022 were collected from various sources, including archives at the Ministry of Agriculture, peer-reviewed journal articles, conference proceedings, reference laboratory reports, and unpublished scientific reports. The data were compiled and analyzed using MS Office Excel® and SPSS® software. Results: A total of 22,690 FMD cases and 1,141 outbreaks were reported in Algeria between 2014 and 2022. The apex of infections occurred in 2014 (34.5%), followed by an increase in the number of infections in 2019 and 2017. The prevalence of FMD extended to 91.6% of the districts of the country, particularly in the north (center) and eastern regions. Cows were the most affected, with 654 outbreaks and more than 3,665 cases. Although FMD affected all four regions, there was a statistically significant long-term decline in the incidence. Conclusion: These spatial and temporal trends underscore the robust disease control methodologies implemented by the Algerian government, such as the strategic immunization of livestock to fortify their viral resistance, stringent constraints on animal mobility, and enlightenment of farmers regarding the hazards associated with unrestricted livestock movement to effectively curb FMD dissemination.
Introduction: The inducible form of nitric oxide (iNOS) is induced by cytokines and endotoxins. T... more Introduction: The inducible form of nitric oxide (iNOS) is induced by cytokines and endotoxins. The cardiac-protective effects of nitric oxide (NO) secreted by endothelial NOS are dependent on arginine. Arginine production occurs mainly within the organism, with the kidneys playing a key role in its synthesis and the elimination of asymmetric dimethylarginine (ADM). In the present study the relationship between iNOS, ADMA and left ventricular hypertrophy in chronic kidney disease (CKD) patients and the effect of treatment with angiotensin converting enzyme inhibitor (ACEI) associated with vitamin C (Vit C) were investigated. Material and methods: A longitudinal observational study was conducted on 153 patients with CKD. We studied the correlation between the mean values of iNOS and ADMA in CKD patients and its relationship with left ventricular hypertrophy and the benefit of treating these patients with an associated ACEI and Vit C. Results: The mean age of the patients was 58.85 ±12.75 years. The mean values of iNOS and ADMA were 63.92 ± 0.59 µmol/l and 16.77 ±0.91 µmol/l, respectively. These values increased significantly with the degradation of the renal function (p < 0.05). A significant positive correlation was found between the left ventricular mass index (LVMI) and the two markers, ADMA (0.901 and p = 0001) and iNOS (0.718 and p = 0.0001). After 2 years of treatment with Vit C and ACEI, a significant decrease in LVMI was observed. Conclusions: NO secreted by the iNOS system and ADMAs initiates cardiac remodeling to lead to left ventricular hypertrophy and cardiac fibrosis. ACEIs increase the expression and activity of eNOS and decrease iNOS. Vit C prevents oxidative damage by scavenging ROS species and reagents nitrogen while. iNOS and ADMA accelerate cardiac aging. We conclude that ACEIs combined with Vit C may improve heart health and limite left ventricular hypertrophy in CKD patients.
The vaginal microbiota has been studied in animal reproduction and fertility, in particular littl... more The vaginal microbiota has been studied in animal reproduction and fertility, in particular little information of vaginal microbes in reference to bovine reproduction and pheromone production is known. The vaginal mucosa in healthy cow is colonized by an equilibrated and dynamic composition of aerobic, facultative anaerobic and obligate anaerobic microbes. Cervico-vaginal mucus (CVM) composition, viscosity and volume vary with the cyclicity and health status of the reproductive tract. In addition, CVM contains pheromones, volatile compounds, and proteins that attract males for coitus. Commensal microbiota plays a key role in protection of the genital tract from pathogenic microbes by competition effect. In the bovine species, the microbial composition, its abundance and diversity in the female gut, vagina, urine, saliva, and feces, and the associated chemical communication remains poorly documented. The impact of microbes in the reproductive tract of cow, buffalo and certain mammals are discussed in this review. Since the microbial population diversity of CVM is modified during estrus phase it presumes that it may have a role for pheromone production in conspecific. Herein, we would like to critically discuss the current state of knowledge on microbially produced signals in animals and the role of genital and CVM microbiota in estrous cycle and pregnancy.
Objective: To study the putative effects of Advanced Oxidation Protein Products (AOPPs) and Advan... more Objective: To study the putative effects of Advanced Oxidation Protein Products (AOPPs) and Advanced Glycation End Products (AGEs) in the development and progression of cardiovascular disease (CVD). Methodology: AGEs, AOPPs, e-NOS, lipid profile, circulating stress and inflammatory biomarkers were evaluated among fifty cardiovascular patients and fifty controls. Independent student's t-test was done for statistical analysis. Results: The malondialdehyde mean level in CVD patients (5.45 nmol/ml) was significantly higher than control (1.36 nmol/ml) (p value = 0.018). Nitric oxide in CVD patients (55.72 ng/ml) was remarkably increased as compared to normal subjects (19.19 ng/ml). A significant change in the mean serum level of AGEs in CVD patients (2.74 ng/ml) and normal individuals (0.85 ng/ml) was recorded (p value = 0.000). The AOPPs also showed significant increased levels in CVD group (132.07 ng/ml) in comparison with normal subjects (83.05 ng/ml) (p value = 0.011). The mean eNOS serum level in CVD group (15.50 U/L) was higher than control group (11.28 U/L) (p value = 0.004). Cardiovascular disease patients, in comparison with healthy controls, showed increased level of total cholesterol (5.48 mmol/L vs 4.45 mmol/L), triglycerides (2.59 mmol/L vs 1.24 mmol/L), and low density lipoprotein (2.47 mmol/L vs 2.31 mmol/L) along with decrease in high density lipoprotein (1.39 mmol/L vs 1.74 mmol/L). The mean MMP-11 serum levels in CVD group (98.69 ng/ml) was almost double of control group (45.60 ng/ml) (p value = 0.017). The mean serum level of TNF-a and IL1-a were 32.16 pg/ml and 6.64 pg/ml in CVD patient. The significant decreasing trend of SOD (p value = 0.041), CAT (p value = 0.018), GSH (p value = 0.036) and GRx (p value = 0.029) but increasing drift of GPx (0.023) level was observed in CVD patients.
Microbiota regulate endometrial health in cattle. It is important to know what a ‘good’ microbiom... more Microbiota regulate endometrial health in cattle. It is important to know what a ‘good’ microbiome is, in order to understand pathogeneses of uterine disease. Given that microbial influx into the genital tract of cows at calving is unavoidable, exploring the involvement of genital tract bacteria in promoting endometrial health is warranted. The dysbiosis of endometrial microbiota is associated with benign and malign uterine diseases. The present review discusses current knowledge about the altered endometrial microbiome and the implications of this modulation on endometrial inflammation, ovarian activity, fecundation, pregnancy, and postpartum complications. Intravaginal administration of symbiotic microbes in cattle is a realistic alternative to antibiotic and hormone therapy to treat uterine disease. Genital microbial diversity can be modeled by nutrition, as the energy balance would improve the growth of specific microbial populations. It may be that probiotics that alter the end...
Clinical endometritis (CE) is a multifactorial disease of dairy animals. Retained fetal membranes... more Clinical endometritis (CE) is a multifactorial disease of dairy animals. Retained fetal membranes (RFM) and metritis are the major risk factors of CE in dairy cows. Because uterine inflammation affects the profitability of the dairy industry, antibiotics and hormonal therapies are commonly used to mitigate against the disease. However, the One-Health concept aims to reduce antibiotic use in food animals to avoid the emergence of drug resistance or residues in milk or meat. Thus, phytotherapy may represent a good alternative to antibiotics in food animals. Echinops spinosus (E. spinosus) is a natural plant known to have therapeutic, anti-inflammatory, antimicrobial, and wound-healing properties in vitro. The aim of the present study was to investigate the efficacy of E. spinosus as a preventive strategy for CE in dairy cows with other postpartum complications. Holstein–Friesian cows (n = 36) diagnosed with RFM or metritis enrolled in the study were allocated into three groups. One gr...
Cattle have a genital microbiome that is established early in life, even before calving. Microbia... more Cattle have a genital microbiome that is established early in life, even before calving. Microbial influx into the reproductive system of cows, during calving or mating, is unavoidable and is likely to alter the commensal microflora composition. It is now well established that a commensal endometrial flora is largely responsible for the overall fertility of cows. These microbes are important for maintenance of structural integrity of the genital mucosal barrier, immunomodulation, and protection against pathogens. Further, the genital microbiome functions in the semiochemical communication between a male and female. An optimal balance between the abundance and diversity of the microbiome is essential to promote female genital tract health. Disruption of this balance leads to dysbiosis and genital diseases and perturbed fertility. As part of the global strategy of One World, One Health, there is a need to reduce antibiotic use in animals. This area of research has the potential to exp...
Table S4. Lists of all the DEGs from this study that were shared in common with the previous glob... more Table S4. Lists of all the DEGs from this study that were shared in common with the previous global bovine salivary proteome analysis by Ang et al. (DOCX 12 kb)
Archives of Medical Sciences. Atherosclerotic Diseases, 2020
Introduction The atherosclerosis process is highly accelerated in patients with chronic kidney di... more Introduction The atherosclerosis process is highly accelerated in patients with chronic kidney disease (CKD). Oxidative stress is considered as one of the pro-atherogenic factors involved in accelerating the atherosclerosis process of the carotid artery. The aim of the present study was to determine the relationship between oxidative stress markers and the progression of carotid atherosclerosis in CKD patients. Material and methods The study was conducted on 162 patients with CKD and 40 controls, and the disease stage was scored between 2 and 5D. Blood samples were taken and advanced oxidative protein product, myeloperoxidases, malondialdehyde, nitric oxide, glutathione, and oxidised low-density lipoprotein were measured. Furthermore, we studied the correlations between these biomarkers and clinical and para-clinical cardiovascular complications. Results The average age of patients was 56.5 years. The oxidative stress markers average ± SD levels in CKD groups compared to the control...
Background and Aims Advanced Oxidation Protein Product (AOPP) are created during oxidative stress... more Background and Aims Advanced Oxidation Protein Product (AOPP) are created during oxidative stress and are considered as markers of this phenomenon and of inflammation. Vitamin E (Vit-E) is a powerful antioxidant, whoever no consensus on its effectiveness on the level of AOPP or the process of atherosclerosis have been made. The aim of the present study was to determine AOPP levels in patients with chronic kidney disease (CKD) at different stages and their relationship with the progression of atherosclerosis in patients under Vit-E treatment. Method A longitudinal study has been conducted on 205 patients with CKD and 40 controls. the correlations between AOPP and glomerular filtration rate (GFR) and different biological markers were analyzed. Doppler of the supraoptic trunks was conducted to assess the correlation of AOPP with intima-media thickness. The effectiveness of Vit-E treatment on AOPP levels and atherosclerosis progression was also investigated. Results AOPP levels were sig...
Clinical endometritis (CE) is a serious disease leading to poor reproductive performances in lact... more Clinical endometritis (CE) is a serious disease leading to poor reproductive performances in lactating dairy cows, thus diminishing farm profitability. To preserve optimum reproductive efficiency, various strategies and therapeutic approaches have been proposed to manage cows with CE, often with contradictory results. Thus, investigating new paths to CE treatment is economically important. The aim of the present study was to test the efficacy of three therapeutic protocols on the clinical cure rate of CE, and improvement of reproductive performance. Cows with CE (n=42), 21–38 days in milk (DIM), were assigned to three treatment groups: PGF: cows (n=19) were treated systemically with two doses of d-cloprostenol, a PGF2α analogue, at 14-days intervals; CEFAX: cows (n=10) received an intrauterine infusion of the combined antibiotics Cefacetrile and Rifaximin; and NAX: cows (n=13) received systemic treatment with Ceftiofur crystalline free acid (CCFA). A control group included cows (n=3...
Aim: The present study was conducted to detect and identify Helicobacter pylori within local cow ... more Aim: The present study was conducted to detect and identify Helicobacter pylori within local cow breeds in the central region of Algeria. Materials and Methods: Two hundred (n=200) cows from three provinces of the central region of Algeria were studied, between January 2016 and September 2017. Each cow was subject to stool, milk, and blood sampling. Milk and fecal samples were used to detect and identify H. pylori using bacteriology culture method. Blood and milk samples were used to detect H. pylori immunoglobulin G (IgG) antibody using enzyme-linked immunosorbent assay. Polymerase chain reaction was used to confirm the abundance of H. pylori in milk by detecting glmM gene. Results: Out of 200 sera and 200 milk samples, 12% (24) and 4% (8/200) were positive for the H. pylori IgG antibody. glmM gene was detected in the milk of 13% of cows and was confirmed in all cows presenting IgG in milk. Conclusion: From the present study, we concluded that the glmM gene is an important marker for detecting H. pylori in milk. Moreover, Algerian local-breed cows are a source of H. pylori and could be responsible for serious zoonosis.
Tropical animal health and production, Jun 1, 2024
cattle, pig, horses and small ruminants) as well as poultry where the economic importance is undo... more cattle, pig, horses and small ruminants) as well as poultry where the economic importance is undoubted (Almeida et al. 2021; Almeida et al. 2017; Ferlizza et al. 2015; Ribeiro et al. 2020). A main advantage of proteomics is the provision of a global view of protein expression patterns in an organism. This is critical for identifying new biomarkers, drug targets, and pathways involved in different biological processes (Petricoin and Liotta 2003). The tropics and the subtropics, being regions facing inherent local challenges, are particularly important in the framework of animal production, given the fact that the vast majority of the human population inhabits these regions. Over the last two decades, mass spectrometry (MS)based techniques have become prominent and a preferred approach for accurately and comprehensively identifying and quantifying proteins in biological samples (Schubert et al. 2017). These methods have played a pivotal role in advancing our comprehension of cellular signalling networks, shedding light on the intricate dynamics of protein-protein interactions across various cellular states, and enhancing our diagnostic capabilities and molecular insights into the mechanisms underlying diseases. Distinguishing between high-quality and low-quality proteomics Mounir Adnane
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Background and Aim: Foot-and-mouth disease (FMD), a major transboundary animal ailment in Algeria... more Background and Aim: Foot-and-mouth disease (FMD), a major transboundary animal ailment in Algeria, is a serious economic burden on the livestock sector. This study aimed to investigate the spatiotemporal distribution of FMD in Algeria and identifies the factors contributing to this phenomenon. Materials and Methods: Data on FMD cases occurring in Algeria from 2014 to 2022 were collected from various sources, including archives at the Ministry of Agriculture, peer-reviewed journal articles, conference proceedings, reference laboratory reports, and unpublished scientific reports. The data were compiled and analyzed using MS Office Excel® and SPSS® software. Results: A total of 22,690 FMD cases and 1,141 outbreaks were reported in Algeria between 2014 and 2022. The apex of infections occurred in 2014 (34.5%), followed by an increase in the number of infections in 2019 and 2017. The prevalence of FMD extended to 91.6% of the districts of the country, particularly in the north (center) and eastern regions. Cows were the most affected, with 654 outbreaks and more than 3,665 cases. Although FMD affected all four regions, there was a statistically significant long-term decline in the incidence. Conclusion: These spatial and temporal trends underscore the robust disease control methodologies implemented by the Algerian government, such as the strategic immunization of livestock to fortify their viral resistance, stringent constraints on animal mobility, and enlightenment of farmers regarding the hazards associated with unrestricted livestock movement to effectively curb FMD dissemination.
Introduction: The inducible form of nitric oxide (iNOS) is induced by cytokines and endotoxins. T... more Introduction: The inducible form of nitric oxide (iNOS) is induced by cytokines and endotoxins. The cardiac-protective effects of nitric oxide (NO) secreted by endothelial NOS are dependent on arginine. Arginine production occurs mainly within the organism, with the kidneys playing a key role in its synthesis and the elimination of asymmetric dimethylarginine (ADM). In the present study the relationship between iNOS, ADMA and left ventricular hypertrophy in chronic kidney disease (CKD) patients and the effect of treatment with angiotensin converting enzyme inhibitor (ACEI) associated with vitamin C (Vit C) were investigated. Material and methods: A longitudinal observational study was conducted on 153 patients with CKD. We studied the correlation between the mean values of iNOS and ADMA in CKD patients and its relationship with left ventricular hypertrophy and the benefit of treating these patients with an associated ACEI and Vit C. Results: The mean age of the patients was 58.85 ±12.75 years. The mean values of iNOS and ADMA were 63.92 ± 0.59 µmol/l and 16.77 ±0.91 µmol/l, respectively. These values increased significantly with the degradation of the renal function (p < 0.05). A significant positive correlation was found between the left ventricular mass index (LVMI) and the two markers, ADMA (0.901 and p = 0001) and iNOS (0.718 and p = 0.0001). After 2 years of treatment with Vit C and ACEI, a significant decrease in LVMI was observed. Conclusions: NO secreted by the iNOS system and ADMAs initiates cardiac remodeling to lead to left ventricular hypertrophy and cardiac fibrosis. ACEIs increase the expression and activity of eNOS and decrease iNOS. Vit C prevents oxidative damage by scavenging ROS species and reagents nitrogen while. iNOS and ADMA accelerate cardiac aging. We conclude that ACEIs combined with Vit C may improve heart health and limite left ventricular hypertrophy in CKD patients.
The vaginal microbiota has been studied in animal reproduction and fertility, in particular littl... more The vaginal microbiota has been studied in animal reproduction and fertility, in particular little information of vaginal microbes in reference to bovine reproduction and pheromone production is known. The vaginal mucosa in healthy cow is colonized by an equilibrated and dynamic composition of aerobic, facultative anaerobic and obligate anaerobic microbes. Cervico-vaginal mucus (CVM) composition, viscosity and volume vary with the cyclicity and health status of the reproductive tract. In addition, CVM contains pheromones, volatile compounds, and proteins that attract males for coitus. Commensal microbiota plays a key role in protection of the genital tract from pathogenic microbes by competition effect. In the bovine species, the microbial composition, its abundance and diversity in the female gut, vagina, urine, saliva, and feces, and the associated chemical communication remains poorly documented. The impact of microbes in the reproductive tract of cow, buffalo and certain mammals are discussed in this review. Since the microbial population diversity of CVM is modified during estrus phase it presumes that it may have a role for pheromone production in conspecific. Herein, we would like to critically discuss the current state of knowledge on microbially produced signals in animals and the role of genital and CVM microbiota in estrous cycle and pregnancy.
Objective: To study the putative effects of Advanced Oxidation Protein Products (AOPPs) and Advan... more Objective: To study the putative effects of Advanced Oxidation Protein Products (AOPPs) and Advanced Glycation End Products (AGEs) in the development and progression of cardiovascular disease (CVD). Methodology: AGEs, AOPPs, e-NOS, lipid profile, circulating stress and inflammatory biomarkers were evaluated among fifty cardiovascular patients and fifty controls. Independent student's t-test was done for statistical analysis. Results: The malondialdehyde mean level in CVD patients (5.45 nmol/ml) was significantly higher than control (1.36 nmol/ml) (p value = 0.018). Nitric oxide in CVD patients (55.72 ng/ml) was remarkably increased as compared to normal subjects (19.19 ng/ml). A significant change in the mean serum level of AGEs in CVD patients (2.74 ng/ml) and normal individuals (0.85 ng/ml) was recorded (p value = 0.000). The AOPPs also showed significant increased levels in CVD group (132.07 ng/ml) in comparison with normal subjects (83.05 ng/ml) (p value = 0.011). The mean eNOS serum level in CVD group (15.50 U/L) was higher than control group (11.28 U/L) (p value = 0.004). Cardiovascular disease patients, in comparison with healthy controls, showed increased level of total cholesterol (5.48 mmol/L vs 4.45 mmol/L), triglycerides (2.59 mmol/L vs 1.24 mmol/L), and low density lipoprotein (2.47 mmol/L vs 2.31 mmol/L) along with decrease in high density lipoprotein (1.39 mmol/L vs 1.74 mmol/L). The mean MMP-11 serum levels in CVD group (98.69 ng/ml) was almost double of control group (45.60 ng/ml) (p value = 0.017). The mean serum level of TNF-a and IL1-a were 32.16 pg/ml and 6.64 pg/ml in CVD patient. The significant decreasing trend of SOD (p value = 0.041), CAT (p value = 0.018), GSH (p value = 0.036) and GRx (p value = 0.029) but increasing drift of GPx (0.023) level was observed in CVD patients.
Microbiota regulate endometrial health in cattle. It is important to know what a ‘good’ microbiom... more Microbiota regulate endometrial health in cattle. It is important to know what a ‘good’ microbiome is, in order to understand pathogeneses of uterine disease. Given that microbial influx into the genital tract of cows at calving is unavoidable, exploring the involvement of genital tract bacteria in promoting endometrial health is warranted. The dysbiosis of endometrial microbiota is associated with benign and malign uterine diseases. The present review discusses current knowledge about the altered endometrial microbiome and the implications of this modulation on endometrial inflammation, ovarian activity, fecundation, pregnancy, and postpartum complications. Intravaginal administration of symbiotic microbes in cattle is a realistic alternative to antibiotic and hormone therapy to treat uterine disease. Genital microbial diversity can be modeled by nutrition, as the energy balance would improve the growth of specific microbial populations. It may be that probiotics that alter the end...
Clinical endometritis (CE) is a multifactorial disease of dairy animals. Retained fetal membranes... more Clinical endometritis (CE) is a multifactorial disease of dairy animals. Retained fetal membranes (RFM) and metritis are the major risk factors of CE in dairy cows. Because uterine inflammation affects the profitability of the dairy industry, antibiotics and hormonal therapies are commonly used to mitigate against the disease. However, the One-Health concept aims to reduce antibiotic use in food animals to avoid the emergence of drug resistance or residues in milk or meat. Thus, phytotherapy may represent a good alternative to antibiotics in food animals. Echinops spinosus (E. spinosus) is a natural plant known to have therapeutic, anti-inflammatory, antimicrobial, and wound-healing properties in vitro. The aim of the present study was to investigate the efficacy of E. spinosus as a preventive strategy for CE in dairy cows with other postpartum complications. Holstein–Friesian cows (n = 36) diagnosed with RFM or metritis enrolled in the study were allocated into three groups. One gr...
Cattle have a genital microbiome that is established early in life, even before calving. Microbia... more Cattle have a genital microbiome that is established early in life, even before calving. Microbial influx into the reproductive system of cows, during calving or mating, is unavoidable and is likely to alter the commensal microflora composition. It is now well established that a commensal endometrial flora is largely responsible for the overall fertility of cows. These microbes are important for maintenance of structural integrity of the genital mucosal barrier, immunomodulation, and protection against pathogens. Further, the genital microbiome functions in the semiochemical communication between a male and female. An optimal balance between the abundance and diversity of the microbiome is essential to promote female genital tract health. Disruption of this balance leads to dysbiosis and genital diseases and perturbed fertility. As part of the global strategy of One World, One Health, there is a need to reduce antibiotic use in animals. This area of research has the potential to exp...
Table S4. Lists of all the DEGs from this study that were shared in common with the previous glob... more Table S4. Lists of all the DEGs from this study that were shared in common with the previous global bovine salivary proteome analysis by Ang et al. (DOCX 12 kb)
Archives of Medical Sciences. Atherosclerotic Diseases, 2020
Introduction The atherosclerosis process is highly accelerated in patients with chronic kidney di... more Introduction The atherosclerosis process is highly accelerated in patients with chronic kidney disease (CKD). Oxidative stress is considered as one of the pro-atherogenic factors involved in accelerating the atherosclerosis process of the carotid artery. The aim of the present study was to determine the relationship between oxidative stress markers and the progression of carotid atherosclerosis in CKD patients. Material and methods The study was conducted on 162 patients with CKD and 40 controls, and the disease stage was scored between 2 and 5D. Blood samples were taken and advanced oxidative protein product, myeloperoxidases, malondialdehyde, nitric oxide, glutathione, and oxidised low-density lipoprotein were measured. Furthermore, we studied the correlations between these biomarkers and clinical and para-clinical cardiovascular complications. Results The average age of patients was 56.5 years. The oxidative stress markers average ± SD levels in CKD groups compared to the control...
Background and Aims Advanced Oxidation Protein Product (AOPP) are created during oxidative stress... more Background and Aims Advanced Oxidation Protein Product (AOPP) are created during oxidative stress and are considered as markers of this phenomenon and of inflammation. Vitamin E (Vit-E) is a powerful antioxidant, whoever no consensus on its effectiveness on the level of AOPP or the process of atherosclerosis have been made. The aim of the present study was to determine AOPP levels in patients with chronic kidney disease (CKD) at different stages and their relationship with the progression of atherosclerosis in patients under Vit-E treatment. Method A longitudinal study has been conducted on 205 patients with CKD and 40 controls. the correlations between AOPP and glomerular filtration rate (GFR) and different biological markers were analyzed. Doppler of the supraoptic trunks was conducted to assess the correlation of AOPP with intima-media thickness. The effectiveness of Vit-E treatment on AOPP levels and atherosclerosis progression was also investigated. Results AOPP levels were sig...
Clinical endometritis (CE) is a serious disease leading to poor reproductive performances in lact... more Clinical endometritis (CE) is a serious disease leading to poor reproductive performances in lactating dairy cows, thus diminishing farm profitability. To preserve optimum reproductive efficiency, various strategies and therapeutic approaches have been proposed to manage cows with CE, often with contradictory results. Thus, investigating new paths to CE treatment is economically important. The aim of the present study was to test the efficacy of three therapeutic protocols on the clinical cure rate of CE, and improvement of reproductive performance. Cows with CE (n=42), 21–38 days in milk (DIM), were assigned to three treatment groups: PGF: cows (n=19) were treated systemically with two doses of d-cloprostenol, a PGF2α analogue, at 14-days intervals; CEFAX: cows (n=10) received an intrauterine infusion of the combined antibiotics Cefacetrile and Rifaximin; and NAX: cows (n=13) received systemic treatment with Ceftiofur crystalline free acid (CCFA). A control group included cows (n=3...
Aim: The present study was conducted to detect and identify Helicobacter pylori within local cow ... more Aim: The present study was conducted to detect and identify Helicobacter pylori within local cow breeds in the central region of Algeria. Materials and Methods: Two hundred (n=200) cows from three provinces of the central region of Algeria were studied, between January 2016 and September 2017. Each cow was subject to stool, milk, and blood sampling. Milk and fecal samples were used to detect and identify H. pylori using bacteriology culture method. Blood and milk samples were used to detect H. pylori immunoglobulin G (IgG) antibody using enzyme-linked immunosorbent assay. Polymerase chain reaction was used to confirm the abundance of H. pylori in milk by detecting glmM gene. Results: Out of 200 sera and 200 milk samples, 12% (24) and 4% (8/200) were positive for the H. pylori IgG antibody. glmM gene was detected in the milk of 13% of cows and was confirmed in all cows presenting IgG in milk. Conclusion: From the present study, we concluded that the glmM gene is an important marker for detecting H. pylori in milk. Moreover, Algerian local-breed cows are a source of H. pylori and could be responsible for serious zoonosis.
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