International Journal of Electrical and Electronics Engineering
Energy is a fundamental necessity for human life and the cornerstone of modern industrial economi... more Energy is a fundamental necessity for human life and the cornerstone of modern industrial economies. In our increasingly connected world, the Internet of Things (IoT) has emerged as a transformative force across various sectors, including energy management. This paper explores the deployment and impact of the Energy wastage controlling and monitoring system, an IoT-based solution, in modern buildings in Mogadishu, Somalia, where energy efficiency is critical due to limited energy resources and high costs. The research reveals the system's capacity to significantly reduce energy consumption by strategically employing motion detection sensors to automate lighting, heating, and cooling based on occupancy and environmental data. Additionally, integrating temperature and humidity sensors contributes to maintaining optimal indoor conditions, enhancing occupant comfort, and curbing unnecessary energy use. A dedicated mobile application empowers users with real-time access to sensor data and remote control over connected devices, promoting active engagement in energy-saving practices. The system effectively addresses phantom power consumption by powering down electrical sockets during non-working hours, resulting in substantial energy savings. Beyond environmental benefits, reduced energy consumption translates into tangible cost savings for building owners and occupants, making buildings more economically viable and attractive. The system's modular design allows for scalability and adaptability to various building types and sizes, fostering tailored energy-efficient solutions. Despite initial setup costs and maintenance challenges, the Energy Wastage Controlling and Monitoring System signifies a significant stride toward achieving energy efficiency in Mogadishu and similar urban environments. It offers an intelligent and automated approach to energy management, contributing to sustainability, cost savings, and occupant comfort. Furthermore, this research underscores the broader implications of IoT-based energy management, emphasizing its potential to address energy challenges in emerging urban centres worldwide. As ongoing research and development efforts persist, IoT technology is poised to shape the future of smart buildings and promote sustainable energy practices, heralding a more efficient and environmentally conscious end.
The major food borne pathogen Campylobacter jejuni utilizes chemotaxis to colonise the chicken ga... more The major food borne pathogen Campylobacter jejuni utilizes chemotaxis to colonise the chicken gastrointestinal tract. Similar to the Escherichia coli chemotaxis system, C. jejuni produces a CheA-CheW-CheY backbone that transduces signals from surface chemoreceptors to direct flagellar rotational direction. In contrast to E. coli, C. jejuni also expresses CheV and CheA has a response regulator domain. The phosphorylation level of CheY in E. coli is modulated by the phosphatase CheZ, however until recently C. jejuni was thought to lack a CheZ homologue. The Hp0170 protein of Helicobacter pylori was shown to be a CheZ remote homologue. As C. jejuni Cj0700 has homology with HP0170, it was postulated that Cj0700 acts as a CheZ orthologue in campylobacters. The aim of this project was to characterise the role of Cj0700 in C. jejuni chemotaxis and demonstrate the phosphatase activity of the protein on C. jejuni CheY, CheV and the response regulator domain of CheA (CheA-RR). A mutant (Δcj0700) and the cognate complement (Δcj0700, Cj0046::cj0700) were constructed in C. jejuni NCTC 11168. No growth differences were seen between Δcj0700, ΔcheY and wild-type. On semi-solid agar the Δcj0700 mutant strain showed reduced motility relative to the wild-type and this phenotype was reversed in the complemented strain. The effect of a mutation in cj0700 was also demonstrated in C. jejuni strains 81-176 and 81116. Cj0700 was expressed as a His-tagged protein and was found to dephosphorylate C. jejuni CheY protein. Expressed Cj0700 also dephosphorylated CheA-RR-P and CheV-P, but less efficiently than CheY-P. The ability of Cj0700 to interact with CheY, CheA-RR and CheV was investigated using an in vitro pull down assay and also in a two hybrid system in E. coli. Using these approaches Cj0700 was shown to interact with CheY, CheA-RR and CheV, with the strength of interaction correlating with the observed cognate phosphatase activity. Although attempts to crystallise Cj0700 were not successful, a combination of bioinformatics, CD and NMR approaches indicated that expressed Cj0700 contains significant levels of disorder. These findings indicate that cj0700 has a role in C. jejuni chemotaxis and shows that Cj0700 can promote dephosphorylation of CheY and hence is likely to be a CheZ orthologue. Furthermore, Cj0700 also modulates the phosphorylation level of the response regulator domain on CheA and may also affect the related domain in CheV
The major food-borne pathogenCampylobacter jejuniemploys chemotactic motility to colonise the avi... more The major food-borne pathogenCampylobacter jejuniemploys chemotactic motility to colonise the avian gut, and also as a virulence mechanism in human diarrhoeal disease.InEscherichia coliCheY activity is modulated by CheZ, a phosphatase originally thought to be absent inC. jejuni. The Hp0170 protein ofHelicobacter pyloriis a distant homologue of CheZ and, asC. jejuniCj0700 is homologous to HP0170, Cj0700 could also act as a CheZ orthologue inCampylobacter. Both theC. jejuniCheV and CheA proteins also contain a response regulator (RR) domain that may be phosphorylated. Cj0700 would therefore be predicted to dephosphorylateC. jejuniCheY and possibly also the CheV and CheA RR domains.A mutant (Δcj0700)and complement (Δcj0700, cj0046::cj0700) were constructed inC.jejunistrains NCTC11168, NCTC11828 and 81-176. On semisolid agar the Δcj0700mutant strain showed reduced motility relative to wild-type and this phenotype was reversed in the complemented strain. In pull down and bacterial two hy...
International Journal of Electrical and Electronics Engineering
Energy is a fundamental necessity for human life and the cornerstone of modern industrial economi... more Energy is a fundamental necessity for human life and the cornerstone of modern industrial economies. In our increasingly connected world, the Internet of Things (IoT) has emerged as a transformative force across various sectors, including energy management. This paper explores the deployment and impact of the Energy wastage controlling and monitoring system, an IoT-based solution, in modern buildings in Mogadishu, Somalia, where energy efficiency is critical due to limited energy resources and high costs. The research reveals the system's capacity to significantly reduce energy consumption by strategically employing motion detection sensors to automate lighting, heating, and cooling based on occupancy and environmental data. Additionally, integrating temperature and humidity sensors contributes to maintaining optimal indoor conditions, enhancing occupant comfort, and curbing unnecessary energy use. A dedicated mobile application empowers users with real-time access to sensor data and remote control over connected devices, promoting active engagement in energy-saving practices. The system effectively addresses phantom power consumption by powering down electrical sockets during non-working hours, resulting in substantial energy savings. Beyond environmental benefits, reduced energy consumption translates into tangible cost savings for building owners and occupants, making buildings more economically viable and attractive. The system's modular design allows for scalability and adaptability to various building types and sizes, fostering tailored energy-efficient solutions. Despite initial setup costs and maintenance challenges, the Energy Wastage Controlling and Monitoring System signifies a significant stride toward achieving energy efficiency in Mogadishu and similar urban environments. It offers an intelligent and automated approach to energy management, contributing to sustainability, cost savings, and occupant comfort. Furthermore, this research underscores the broader implications of IoT-based energy management, emphasizing its potential to address energy challenges in emerging urban centres worldwide. As ongoing research and development efforts persist, IoT technology is poised to shape the future of smart buildings and promote sustainable energy practices, heralding a more efficient and environmentally conscious end.
The major food borne pathogen Campylobacter jejuni utilizes chemotaxis to colonise the chicken ga... more The major food borne pathogen Campylobacter jejuni utilizes chemotaxis to colonise the chicken gastrointestinal tract. Similar to the Escherichia coli chemotaxis system, C. jejuni produces a CheA-CheW-CheY backbone that transduces signals from surface chemoreceptors to direct flagellar rotational direction. In contrast to E. coli, C. jejuni also expresses CheV and CheA has a response regulator domain. The phosphorylation level of CheY in E. coli is modulated by the phosphatase CheZ, however until recently C. jejuni was thought to lack a CheZ homologue. The Hp0170 protein of Helicobacter pylori was shown to be a CheZ remote homologue. As C. jejuni Cj0700 has homology with HP0170, it was postulated that Cj0700 acts as a CheZ orthologue in campylobacters. The aim of this project was to characterise the role of Cj0700 in C. jejuni chemotaxis and demonstrate the phosphatase activity of the protein on C. jejuni CheY, CheV and the response regulator domain of CheA (CheA-RR). A mutant (Δcj0700) and the cognate complement (Δcj0700, Cj0046::cj0700) were constructed in C. jejuni NCTC 11168. No growth differences were seen between Δcj0700, ΔcheY and wild-type. On semi-solid agar the Δcj0700 mutant strain showed reduced motility relative to the wild-type and this phenotype was reversed in the complemented strain. The effect of a mutation in cj0700 was also demonstrated in C. jejuni strains 81-176 and 81116. Cj0700 was expressed as a His-tagged protein and was found to dephosphorylate C. jejuni CheY protein. Expressed Cj0700 also dephosphorylated CheA-RR-P and CheV-P, but less efficiently than CheY-P. The ability of Cj0700 to interact with CheY, CheA-RR and CheV was investigated using an in vitro pull down assay and also in a two hybrid system in E. coli. Using these approaches Cj0700 was shown to interact with CheY, CheA-RR and CheV, with the strength of interaction correlating with the observed cognate phosphatase activity. Although attempts to crystallise Cj0700 were not successful, a combination of bioinformatics, CD and NMR approaches indicated that expressed Cj0700 contains significant levels of disorder. These findings indicate that cj0700 has a role in C. jejuni chemotaxis and shows that Cj0700 can promote dephosphorylation of CheY and hence is likely to be a CheZ orthologue. Furthermore, Cj0700 also modulates the phosphorylation level of the response regulator domain on CheA and may also affect the related domain in CheV
The major food-borne pathogenCampylobacter jejuniemploys chemotactic motility to colonise the avi... more The major food-borne pathogenCampylobacter jejuniemploys chemotactic motility to colonise the avian gut, and also as a virulence mechanism in human diarrhoeal disease.InEscherichia coliCheY activity is modulated by CheZ, a phosphatase originally thought to be absent inC. jejuni. The Hp0170 protein ofHelicobacter pyloriis a distant homologue of CheZ and, asC. jejuniCj0700 is homologous to HP0170, Cj0700 could also act as a CheZ orthologue inCampylobacter. Both theC. jejuniCheV and CheA proteins also contain a response regulator (RR) domain that may be phosphorylated. Cj0700 would therefore be predicted to dephosphorylateC. jejuniCheY and possibly also the CheV and CheA RR domains.A mutant (Δcj0700)and complement (Δcj0700, cj0046::cj0700) were constructed inC.jejunistrains NCTC11168, NCTC11828 and 81-176. On semisolid agar the Δcj0700mutant strain showed reduced motility relative to wild-type and this phenotype was reversed in the complemented strain. In pull down and bacterial two hy...
Uploads
Papers by Abdullahi Jama