Papers by Cherif ABDENNOUR
The study was designed to investigate the possible protective role of argan oil in mercuric chlor... more The study was designed to investigate the possible protective role of argan oil in mercuric chloride induced renal stress, by using biochemical approaches. The effects of argan oil on mercuric chloride induced oxidative and renal stress were evaluated by serum creatinine, urea and uric acid levels, kidney tissue lipid peroxidation, GSH levels, GSH-Px and GST activities. Administration of mercuric chloride induced significant increase in serum: interleukine 1, interleukine 6 and tumor necrosis factor α (TNFα) levels, creatinine, urea and uric acid concentration showing renal stress. Mercuric chloride also induced oxidative stress, as indicate by decreased kidney tissue of GSH level, GSH-Px and GST activities along with increase the level of lipid peroxidation. Argan oil treatment markedly reduced elevated serum: IL1, IL6, TNFα, creatinine, urea and uric acid levels and counteracted the deterious effects of mercuric chloride on oxidative stress markers changes caused by HgCl 2 in kidney. Our results indicate that argan oil could have a beneficial role against mercuric chloride induced nephrotoxicity and oxidative stress in rat.
Turkish Journal of Agriculture: Food Science and Technology, Nov 9, 2022
Background: Matricaria chamomilla L. is a famous medicinal plant distributed worldwide. It is wid... more Background: Matricaria chamomilla L. is a famous medicinal plant distributed worldwide. It is widely used in traditional medicine to treat all kinds of diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders. It is also used as a sedative, antispasmodic, antiseptic, and antiemetic. Our aims in this study was thus to quantify the phenolic, flavonoids contents in the flower of this plant, and also to evaluate the in vitro antioxidant potential and the in vivo analgesic activity. Methods: The total phenolic and flavonoid contents of the plant aqueous extract (MCAqE) were estimated using the Folin-Ciocalteu and AlCl3 colorimetric methods, respectively. However, DPPH method was used to evaluate the in vitro antioxidant activity. Analgesic activity was tested by acetic acid induced writhing model in mice. Results: Quantitative determination of total polyphenols and flavonoids revealed that this extract contained 158.41±1.6 mg gallic acid equivalent/g of dry extract and 37.06±0.56 mg quercetin equivalent/g of dry extract, respectively. The antioxidant activity of the plant extract was important (IC50=3.08±0.25 mg/mL). MCAqE extract, at 400 mg/kg, showed analgesic activity (39.60±8.70%) against acetic acid induced pain in mice while the standard reference drug Diclofenac sodium exhibited 90.44±2.80% activity at 10 mg/kg dose.
Immunomodulatory activity of Argan oil was evaluated on phagocytic activity by carbon clearance t... more Immunomodulatory activity of Argan oil was evaluated on phagocytic activity by carbon clearance test. Adult Albinos Wistar rats randomly divided into four groups, were the first was served as a control, while animals of treatment group were given Argan oil at dose of: 2.5, 5 and 10 mL kg−1 by gavage respectively 10 days before injected the carbon ink suspension. In carbone clearance test, Argan oil exhibited significantly phagocytic index dose-dependent against control group, indicating stimulation of the reticulo-endothelial system. Present study thus reveals that argan oil holds promise as immunomodulatory agent, which act by stimulating dose dependent phagocytic function.
The study was designed to investigate the possible protective role of argan oil in mercuric chlor... more The study was designed to investigate the possible protective role of argan oil in mercuric chloride induced renal stress, by using biochemical approaches. The effects of argan oil on mercuric chloride induced oxidative and renal stress were evaluated by serum creatinine, urea and uric acid levels, kidney tissue lipid peroxidation, GSH levels, GSH-Px and GST activities. Administration of mercuric chloride induced significant increase in serum: interleukine 1, interleukine 6 and tumor necrosis factor α (TNFα) levels, creatinine, urea and uric acid concentration showing renal stress. Mercuric chloride also induced oxidative stress, as indicate by decreased kidney tissue of GSH level, GSH-Px and GST activities along with increase the level of lipid peroxidation. Argan oil treatment markedly reduced elevated serum: IL1, IL6, TNFα, creatinine, urea and uric acid levels and counteracted the deterious effects of mercuric chloride on oxidative stress markers changes caused by HgCl 2 in kidn...
Biological Trace Element Research, 2021
The current study investigates the potential alleviating activity of bulbs (B) and leaves (L) of ... more The current study investigates the potential alleviating activity of bulbs (B) and leaves (L) of Allium triquetrum aqueous extract (ATE) on repro-toxicity induced by lead acetate (Pb) in male Wistar rats administrated orally for 3 consecutive weeks. Eighteen groups of rats were divided into the control, Pb (500 mg/kg body weight/day), positive controls of B and L (2 g, 3 g, 4 g, 6 g/kg body weight/day), in addition to four mixtures of each of Pb-B (Pb-B1, Pb-B2, Pb-B3, Pb-B4) and Pb-L (Pb-L1, Pb-L2, Pb-L3, Pb-L4). The two extracts were subjected to phytochemical screening and HPLC analysis. Sperm characteristics were evaluated by CASA system, as well as the serum testosterone, testicular and epididymal levels of glutathione (GSH), glutathione peroxidase (GPx), and malondialdehyde (MDA). The phytochemical screening proved that bulbs' and leaves' extracts were rich in various compounds and the HPLC showed that leaves contain more tannins. Results revealed a significant decrease in the testicular and in the epididymal weights, sperm concentration, motility, testosterone, velocity, vitality, round cells, GSH, and GPx levels in the Pb-intoxicated rats compared to the control, with the exception of MDA concentration that was significantly increased. However, the co-administration of garlic extracts (Pb-B and Pb-L) exhibited a significant increase in all mentioned markers, except for the MDA level which was reduced. Likewise, Pb caused histological injuries in the testicular seminiferous of rats, while the co-administration of wild garlic has reduced such effect, especially in the higher doses. Both extracts of Pb-B and Pb-L have attenuated Pb toxicity in a dose-dependent manner. In conclusion, aqueous extracts of A. triquetrum have the potential to reduce Pb testicular injuries by boosting sperm characteristics and ameliorating oxidative stress markers.
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology, 2020
The aim of the current investigation has been to evaluate the toxic effects of Diflubenzuron (DFB... more The aim of the current investigation has been to evaluate the toxic effects of Diflubenzuron (DFB) insecticide on domestic rabbits. In this study, we aimed to evaluate the toxicity of DFB on some reproductive parameters in male Oryctolagus cuniculus rabbits. In animals (6 to 7 months and 1700 ± 170 g) the insecticide was used on three different doses:250,500,750 mg/kg. The insecticide was daily administrated for four weeks of experimental periods. Results showed the following effects as compared with the control group: adecrease in reproductive indicators (concentration, motility, speed, and vitality of spermatozoa) and in serum testosterone concentration. Histological examination showed pathological alterations such as a remarkable reduction in spermatozoa concentration and cellular malformation within seminiferous tubules and epithelial cells especially in groups treated by the highest dose of Diflubenzuron, data demonstrated that DFB caused an effect on reproductive function in adult male rabbits.
New Visions in Biological Science Vol. 6, 2021
Article history: Received 15 April 2014 Received in revised form 22 May 2014 Accepted 25 May 2014... more Article history: Received 15 April 2014 Received in revised form 22 May 2014 Accepted 25 May 2014 Available online 15 June 2014
Article history: Received 4 September 2014 Received in revised form 24 November 2014 Accepted 8 D... more Article history: Received 4 September 2014 Received in revised form 24 November 2014 Accepted 8 December 2014 Available online 16 December 2014
The objective of this work is to use the Hawthorn Crataegus monogyna, as a protective agent again... more The objective of this work is to use the Hawthorn Crataegus monogyna, as a protective agent against copper chronic intoxication. Male Wistar rats were divided into six groups; the control received tap water, standard diet ad libitum, two positive controls treated respectively with Hawthorn leaves and fruits aqueous extract, a group treated with Cu and finally, two groups treated with Cu+leaves (CuL) and Cu+fruits (CuF). The treatment was done by gavage for 30 consecutive days, where: glucose-6-phosphate of erythrocytes (G6PD), white blood cells (WBC), red blood cells (RBC), platelets (PLT), hemoglobin (HGB), hematocrits (HCT), mean corpuscular volume (MCV), triglycerides (TRIG), cholesterol (CHOL), high density lipoproteins (HDL) and low density lipoproteins (LDL) were measured. Copper treatment reduced G6PD, RBC, HGB, HCT, MCV, TRIG and CHOL levels, compared to the control. Compared to the Cu group, the two combined treatments (Cu L and Cu F) have an increase on G6PDH, RBC, HGB, HC...
Annals of Biological Research, 2014
Di-n-butyl phthalate (DBP) is an environmental pollutant, widely used as a plasticizer in various... more Di-n-butyl phthalate (DBP) is an environmental pollutant, widely used as a plasticizer in various products including plastics and cosmetics. Previous studies have revealed that DBP has potential reproductive toxicity in animals and humans. The present study was designed to investigate the effect of DBP on histological, heamatological and reproductive markers in adult male rabbits. Thus, 24 adult male rabbits Oryctolagus cuniculus were divided into four groups. DBP was administered to each group by gavage at doses of 0 (control), 250, 500 and 750 mg/kg/day for a period of 4 weeks. The results revealed a significant reduction in the weight of testes, epididymis and also in the number, speed, mobility and vitality of sperm, accompanied with a significant decrease in morphological changes of flagellum in the treated groups compared to control group. Moreover, a significant decline was observed in the mean of red blood cells counts, haematocrit and hemoglobin in all treated groups compar...
Pharmacognosy Journal, 2021
Background: Medicinal plants are a reservoir of biologically active compounds with therapeutic pr... more Background: Medicinal plants are a reservoir of biologically active compounds with therapeutic properties that, over time, have been used for the treatment of various diseases. This study aims to evaluate the protective effect of the aqueous extract of the leaves of Salvia officinalis against cypermethrin-induced toxicity. Methods: 30 male rats weighing approximately 240 g were divided into equal six groups; the control group received tap water, the positive control received the aqueous extract of sage leaves (SLE) at a dose of (0.5g/kg bw), the groups treated with cypermethrin (Cyp1) at 8.33 mg/kg bw and (Cyp2) at 25 mg/kg bw, and the groups treated by cypermethrin combined with aqueous extract of sage SLE+Cyp1 (0.5g/kg bw+8.33 mg/kg bw) and SLE+Cyp2 (0.5g/kg bw+25 mg/kg bw) for four days/week. After 4 weeks of oral administration, epididymal seminal fluid was analyzed via the CASA system, in addition to the histological study testis and epididymis. Results: The obtained results showed a decrease in the absolute weight of the reproductive organs, with a significant decrease in sperm concentration, motility and speed in the cypermethrin-treated group compared to the control. Histological study of the testes and epididymis indicates an alteration in the stages of spermatogenesis in groups Cyp1 and Cyp2 compared to the control. However, the above-mentioned parameters were maintained almost normal in the groups that received the aqueous extract of sage with both doses of cypermethrin. Conclusion: it can be demonstrated that SLE has been shown to protect rats from cypermethrin-induced reprotoxicity.
Journal of Animal Behaviour and Biometeorology, 2021
The present study investigated the toxic effect of a mixture of three pesticides (cypermethrin, m... more The present study investigated the toxic effect of a mixture of three pesticides (cypermethrin, mancozeb, and metalaxyl) on reproduction and oxidative stress parameters in male Wistar rats. Animals were treated at doses 1/60, 1/30, and 1/10 LD50 of each pesticide daily in the diet for 08 weeks. At the end of the treatment period, animals were sacrificed by decapitation. The results indicate a decrease in the absolute weight of testes and epididymis, the serum of testosterone hormone, and cholesterol levels. These parameters were significant reduced in males exposed to the mixed pesticides. A reduction in sperm concentration, motility, and viability also was observed. Besides, the ingestion of mixed pesticides at all three concentrations caused a significant decrease in GSH, GPx levels and an increase in MDA levels compared to the control group. This was accompanied by histopathological changes in testis and epididymis of rats such as seminiferous tubules degeneration, decreasing number of spermatogenic cells, edema, expansion of interstitial spaces, cell necrosis, and reducing the diameter of the epididymal tube compared to the control group. Thus, we strongly suggest that the mixture of pesticides causes damages to the male reproductive system.
Ankara Universitesi Eczacilik Fakultesi Dergisi, 2020
Objective: The purpose of this study was to investigate the possible beneficial use of common haw... more Objective: The purpose of this study was to investigate the possible beneficial use of common hawthorn Crataegus monogyna aqueous extract at 1.5g/Kg bw/day against chronic copper sulfate intoxication (100mg/Kg bw) in Wistar rats. Material and Method: Animals were divided into 6 groups; the untreated control (C), 2 positive controls treated respectively with hawthorn fruits (F) and leaves (L), 1 group treated with copper (Cu) and 2 combined treatment groups treated with Cu and hawthorn-fruits (CuF) and Cu and hawthorn-leaves (CuL). After 30 days of oral administration, testis weight and plasma testosterone levels were evaluated, in addition to the epididymal sperm concentration, motility, vitality, velocity (VCL, VSL and VAP), the amplitude of lateral head displacement (ALH), and the beat cross frequency (BCF). Testicular glutathione (GSH), malondialdehyde (MDA), and glutathione peroxidase (GPx) were also evaluated.
African Health Sciences, 2020
Background: Nitrate (NO3) is the most common chemical contaminant in the world’s ground water aqu... more Background: Nitrate (NO3) is the most common chemical contaminant in the world’s ground water aquifer. Oxidative stress has been proposed as a possible mechanism involved in NO3 toxicity on non-target organism. Objectives: The current study aimed to elucidate the potential protective effect of Telfairia occidentalis (pumpkin seed oil, PSO) against hepatotoxicity induced by sodium nitrate. Methods: Wistar rats were exposed either to NaNO3 (200 mg/kg bw) in drinking water in drinking water, or to 4ml PSO/kg bw by gavage or to their combination. Oxidative stress parameters, biochemical biomarkers and liver histopathological examination were determined. Results: Our data showed that the exposure of rats to NaNO3 caused significant changes of some haematological parameters compared to the control. In addition, there was a significant elevation of the levels of biochemical markers as that of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and lactate dehydrogena...
Pakistan Journal of Zoology, 2018
Authors' Contribution SS, SH, SS and CS carried out the experimental work. LD performed the histo... more Authors' Contribution SS, SH, SS and CS carried out the experimental work. LD performed the histological study. SS wrote discussion. CA corrected the langusge of the article.
نشریه علمی پژوهشی اقیانوس شناسی, Oct 15, 2010
Journal of Stress Physiology Biochemistry, 2014
Olive oil is beneficial effects are not only related to its high content of oleic acid, but also ... more Olive oil is beneficial effects are not only related to its high content of oleic acid, but also to the antioxidant potential of its polyphenols. In this study, we assess the effects of virgin olive oil on mercuric chloride induced oxidative damage in the liver of rats. Adult male Albinos Wistar rats randomly divided into four groups, were the first was served as a control, whereas the remaining groups respectively treated with: virgin olive oil (2ml/ kg b.w; by gavage), mercuric chloride (0.5 mg/kg body weight i.p) and combination of virgin olive oil and HgCl 2. Change in liver enzyme activities, thiobarbituric acid reactive substances (TBARS) level, antioxidants and reduced glutathione (GSH) contents were determined after 2 weeks of experimental period. Exposure of rats to mercuric chloride caused a significant increase the lipid peroxidation level along with corresponding decrease in the reduced glutathione and various antioxidant enzymes in liver. And increase in serum: glucose level, APL and transaminases activities and decreased in total protein and albumin levels. Furthermore, treatment with mercuric chloride caused a marked elevation of liver weight and decreased body weight. Supplementation of virgin olive oil resulted in decreased of lipid peroxidation level and in the serum: AST, ALT and APL activities were decreased along with increase in total protein, albumin and liver GSH levels. The activities of antioxidants enzymes: glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) were also concomitantly restored to near normal level by virgin olive oil supplementation to mercuric chloride intoxicated rats. Liver histological studies have confirmed the changes observed in biochemical parameters and proved the beneficial role of virgin olive oil. The results clearly demonstrate that virgin olive oil treatment augments the antioxidants defense mechanism in mercuric chloride induced toxicity and provides evidence that it may have a therapeutic role in free radical mediated diseases.
Veterinary World, 2015
The objective of this study was to investigate the possible protective role of nettle Urtica dioi... more The objective of this study was to investigate the possible protective role of nettle Urtica dioica (UD) against Hginduced toxicity. Materials and Methods: A total of 28 rats were equally divided into four groups: the control, the Hg (0.8 g HgCl 2 /kg in the diet), the UD (1.5 ml UD/rat by gavage), and the Hg+UD group. HgCl 2 was daily dissolved in distilled water and immediately mixed with the standard diet. A solution of daily infused fresh nettle leaves in boiling water (16 g in 25 ml) was obtained and then it was administrated by gavage. Biochemical and reproductive markers, in addition to glutathione (GSH) level (liver, kidney and testis) and the histological profiles (testis and epididymis) were evaluated after 1 month exposure. Results: Compared to the control, the levels of glucose, triglycerides, urea, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were significantly raised in the Hg group. In the latter group, the concentrations of Mg, Fe, and Ca were significantly decreased. Besides, Hg+UD group has only showed raised AST activity and reduced Mg level. Concerning the fertility markers, Hg has provoked a significant decrease in the spermatozoa's concentration and motility and in plasma testosterone level as well. Furthermore, hepatic, renal and testicular GSH concentrations have declined significantly in the Hg treated rat compared to the control. A remarkable enhancement of the GSH level was observed in all organs of the UD group. The histological examinations of the Hg group have revealed marked testicular degeneration of the most seminiferous tubules, and showed few sperms in the lumen of epididymis ducts. However, the Hg+UD rats have demonstrated an improved histological structure with the presence of important numbers of sperms in the lumen. In addition, a clear stabilization of organized seminiferous tubules and an increased sperms' numbers were noted in the UD supplemented rats. Conclusion: Nettle leaves have not only played a clear protective role during Hg intoxication, but it also enhanced hepatic, renal and testicular GSH level of Wistar rats.
Emirates Journal of Food and Agriculture, 2016
Recently, the analysis of metal content has been a method of discriminating honey origin, and an ... more Recently, the analysis of metal content has been a method of discriminating honey origin, and an attractive substitute instead of traditional method and some expensive and complex new parameter determinations (De Alda-Garcilope et al., 2012). Different methods are used by many authors to determine mineral and heavy metal contents of honey (Torres et al., The aim of this study is to characterize Honey from semi-arid regions of Northeast Algeria. Therefore, twenty artisanal honey samples (13 multifl oral and 7 unifl oral) were collected and then characterized on the basis of their physico-chemical parameters (free acidity, pH, moisture content, electrical conductivity, Ash and sugar content) and the mineral composition (Ca, K, Mg and Na). Results showed that these regions are characterized by low moisture and low percentage of major elements. However, potassium was the most abundant of all elements determined. A statistical analysis was carried out to identify the most signifi cant parameters and the chemometric methods used such as principal component analysis (PCA) and cluster analysis (CA) in order to classify the three types of honeys. The PCA indicated that the cumulative variance was 81.74% with the fi rst four principal components variables. Therefore, the use of chemometric methods on the physico-chemical parameters and major elements is not a useful tool to characterize different types of honey originates regions with the same climate.
Journal of Applied Sciences Research
The essential (Cu and Zn) and the non-essential (Pb and Ni) trace metal concentrations were studi... more The essential (Cu and Zn) and the non-essential (Pb and Ni) trace metal concentrations were studied in the mussel Donax trunculus from sites exposed to different urban and industrial pollution. Results have indicated that the unpolluted location has recorded the lowest metal levels. However, the concentrations of Pb, Ni, and Zn were significantly higher in site exposed to industrial pollution compared to the other sites. Males have accumulated more nickel than females, from the industrial polluted site, whereas females have higher Cu and Zn compared to males, from the relatively clean site. This study suggests that D. trunculus might be used for trace metal monitoring in Annaba estuaries, and it is not recommended for human consumption in the sites close to Annaba city. Trace metals accumulation pattern in Donax is not only under metal enrichment effect, but it also affected by sex differences.
Uploads
Papers by Cherif ABDENNOUR