Papers by Sergei Spiridonov
Zootaxa
A new nematode species, Heth longquani sp. n. (Hethidae, Ransonematoidea, Rhigonematomorpha, Rhab... more A new nematode species, Heth longquani sp. n. (Hethidae, Ransonematoidea, Rhigonematomorpha, Rhabditida) is described from the camballoid millipede, Chonecambala crassicauda from Thanh Hóa province, Viet Nam. The morphological features of this new species were studied with brightfield and scanning electron microscopies. Partial sequences of LSU rDNA and Cox1 mtDNA are provided. The phylogeny of the nematodes of the genus Heth Cobb, 1898 is discussed.
Zoosystematica Rossica
A new nematomorph species, Chordodes guineensis sp. n., is described from Kankan, Guinea. It is d... more A new nematomorph species, Chordodes guineensis sp. n., is described from Kankan, Guinea. It is distinguished from other Chordodes species in the structures of the areolar layer of cuticle. Presumably, insects of the order Orthoptera are the natural hosts of Ch. guineensis sp. n.
Biocontrol agents: entomopathogenic and slug parasitic nematodes
The entomopathogenic nematodes (EPNs) are representatives of Nematoda, which are characterized by... more The entomopathogenic nematodes (EPNs) are representatives of Nematoda, which are characterized by: (i) adult stages found in nature only inside the cadavers of insects killed and processed by symbiotic bacteria; (ii) infective (= dauer) juveniles being the only free-living stage in soil. Presently, the representatives of two nematode families are regarded as EPNs: Steinernematidae and Heterorhabditidae. The family Heterorhabditidae is monotypic (i.e. contains a single genus, Heterorhabditis), while Steinernematidae contains two genera (Steinernema and exotic Neosteinernema). This chapter focuses on EPN taxonomy and morphology. The chapter presents the diversity of these nematodes in condensed form and offer ways in which to navigate it.
Acta Parasitologica, 2022
PURPOSE The present study investigates the origin of Arion vulgaris slugs in the parks of Moscow ... more PURPOSE The present study investigates the origin of Arion vulgaris slugs in the parks of Moscow city and their parasites. METHODS Snails and slugs inhabiting green areas of Moscow city were collected in the summer season of 2020 and examined on the presence of gastropod-associated nematodes and trematodes using morphological and molecular methods. RESULTS The presence of the alien slug species, Arion vulgaris, was recorded in several locations, and the mitochondrial gene-based analysis has shown that slug populations inhabited Moscow parks originated from West and Central Europe. Out of a total of 15 gastropod species examined, A. vulgaris was the only species infected by the nematode Alloionema appendiculatum Schneider, 1859, a larval parasite of molluscs. It is the first record of this nematode from the territory of the Russian Federation. COX1 mtDNA sequences of A. appendiculatum obtained from 3 populations of infected slugs were identical with those from Western and Central Europe similarly to their gastropod hosts thus indicating that the nematodes travelled with their hosts. No parasites dangerous for humans or animals were found. CONCLUSION The complex life cycle of A. appendiculatum includes a free-living stage in soil which offers a source of infection for other potentially susceptible gastropod species but the capacity of A. appendiculatum to change hosts in local conditions needs to be further investigated. The particular susceptibility and tolerance of A. vulgaris to nematodes in our study was in concordance with earlier data while in contradiction with the enemy release hypothesis.
Russian Journal of Biological Invasions, 2022
is a parasitic nematode specific to reindeer ( ). In the XXth century, reindeer were not found in... more is a parasitic nematode specific to reindeer ( ). In the XXth century, reindeer were not found in the Leningrad Region. However, by 2020, several dozen of them were brought from the Murmansk Region and the Yamalo-Nenets Autonomous Okrug and settled in at least seven private menageries. As a result of a study of the feces of 34 imported reindeer carried out in 2018-2021, larvae of nematodes were isolated, whose morphological, morphometric, physiological and biological characteristics corresponded to those of E. rangiferi . The species identification was confirmed by subsequent molecular genetic studies. The discovery of parasite larvae in the feces of the second generation reindeer (born in 2018-2020) indicates the successful completion of the full life cycle of this helminth, which requires a participation of intermediate hosts - terrestrial or freshwater gastropods. A combination of such circumstances as: 1) the appearance of reindeer infested with E. rangiferi in the Leningrad Reg...
Paracameronia caucasica gen. n. sp. n. living in the hind gut of mole crickets in South Azerbaija... more Paracameronia caucasica gen. n. sp. n. living in the hind gut of mole crickets in South Azerbaijan is described. The genus is distinguished from the similar Cameronia Basir by the formation of egg agglomerates inside the uterus of females, by the form and mode of construction of the egg agglomerates and by the form of spermatozoon and egg shell. Two female - representatives of the same genus Paracameronia gen. n. have been found in mole crickets form Georgia, USSR.
Parasitology Research, 2021
The genetic diversity of Syphacia nematodes (intestinal parasites of rodents) was studied in the ... more The genetic diversity of Syphacia nematodes (intestinal parasites of rodents) was studied in the hybrid zone of two sister species of common voles, Microtus arvalis and Microtus obscurus, in the Oka River valley, east of Moscow. Syphacia nematodes of other rodent species (Microtus rossiaemeridionalis, Alexandromys oeconomus, Sylvaemus uralensis, and Apodemus agrarius) that inhabit the area were also studied. Phylogenetic trees for the studied nematodes were inferred from the analysis of nuclear ITS1+5.8S+ITS2, LSU rDNA, and mitochondrial CO1 gene partial sequences. Syphacia nematodes of the studied area form three well-defined clades in the phylogenetic tree of this genus. Morphological analysis revealed similarities between the obtained sequences with those of known Syphacia species from the GenBank database, which enabled identifying these three clades up to the species level: S. montana, S. agraria, and S. frederici. Russian haplotypes of Syphacia are different from West European and East Asian haplotypes with pronounced genetic distances. A high level of specificity was reported for two of these three species (S. frederici, only in Sylvaemus uralensis; S. agraria, only in Apodemus agrarius). S. montana was found in different species of voles. Remarkably, S. montana specimens from M. arvalis and M. obscurus were genetically uniform, while S. montana, specimens from hybrids between these two species formed a separate clade distant from those originating from non-hybridised hosts.
Parazitologiia, 2016
Acanthocephalans Prosthenorchis cf. elegans were found in primates in the Moscow Zoo. The larvae ... more Acanthocephalans Prosthenorchis cf. elegans were found in primates in the Moscow Zoo. The larvae of these parasites (cistacanths) were found in cockroaches Blattella germanica that had been captured near aviaries of infected animals. Descriptions and drawings of adult parasites and their larvae are given. Analysis of Prosthenorchis cf. elegans genes ITS 1 rDNA and CO 1 mtDNA shows phylogenetic relations of these parasites with several representatives of the class Archiacanthocephala. The obtained molecular data, however, do not support the monophyly of the family Oligacanthorhynchidae and the order Oligacanthorhynchida.
Russian Journal of Nematology, 2011
Russian Journal of Nematology, 1993
Russian Journal of Nematology, 2016
Nematology, 2019
Summary Two new nematode species of Phasmarhabditis were isolated from land snails in North and W... more Summary Two new nematode species of Phasmarhabditis were isolated from land snails in North and West Caucasus, Russian Federation. Phasmarhabditis circassica sp. n. originated from Oxychilus sp. collected by the Nickel settlement in the Adygea Republic while P. clausiliiae sp. n. came from Clausiliidae gen. sp. in the Stavropol district. Phasmarhabditis circassica sp. n. is characterised morphologically by male spicules with a hollow tip, a widely conoid, spicate female tail, ensheathed dauer juveniles 896 (813-982) μm long with a tiny but distinct cuticular apical cap and a long filamentous tail, and exsheathed dauers 773 (670-950) μm long with a tail half as long as in the ensheathed dauers and with a rounded tip. Phasmarhabditis clausiliiae sp. n. is very close to P. circassica sp. n. in having similar morphological traits and is differentiated mainly in having a longer female tail of 94 (79-111) vs 82 (54-125 μm) and the much smaller ratio c of 13.7 (10.3-18.4) vs 22.7 (16.8-27....
Parasitology, 2019
The intestinal parasitic nematode, Baylisascaris transfuga, was recorded in wild rodents for the ... more The intestinal parasitic nematode, Baylisascaris transfuga, was recorded in wild rodents for the first time. Representatives of four murid species (15 Myodes rufocanus, 10 M. rutilus, 3 M. glareolus and 27 Microtus oeconomus) were collected in the White Sea coastal habitats in the south of the Kola Peninsula, Russia in July 2015 and examined for parasites. Encapsulated nematode larvae were detected in the mesentery and the large intestine wall of one grey-sided vole (M. rufocanus) and one tundra vole (M. oeconomus). Based on morphology, the larvae were identified as belonging to the genus Baylisascaris Sprent 1968. The partial 18S rDNA sequence of the larvae from the voles was obtained and fully corresponded to the sequence of Baylisascaris transfuga in the NCBI GenBank. The ITS rDNA and CoxI mtDNA sequences these larvae were also similar to the respective B. transfuga sequences in GenBank. The presence of B. transfuga in wild rodents suggests that rodents can participate in the B. ...
Biology Bulletin, 2016
The informativity of some regions of the ribosomal cluster (rDNA) in freshwater horsehair worms (... more The informativity of some regions of the ribosomal cluster (rDNA) in freshwater horsehair worms (Nematomorpha) has been analyzed to trace the nucleotide differences for discrimination of species and genera of these parasites using available sequences from the NCBI GenBank and our data.
Известия Российской академии наук. Серия биологическая, 2013
PLOS ONE, 2018
The study of parasites in recently established populations of invasive species can shed light on ... more The study of parasites in recently established populations of invasive species can shed light on the sources of invasion and possible indirect interactions between the alien species and native ones. We studied parasites of the global invader Harmonia axyridis (Coleoptera: Coccinellidae) in the Caucasus. In 2012, the first established population of Ha. axyridis was recorded in the Caucasus in Sochi (south of European Russia, Black Sea coast). By 2018, the ladybird had spread to a vast area: Armenia, Georgia and south Russia (Adygea, the Krasnodar territory, the Stavropol territory, Dagestan, Kabardino-Balkaria and North Ossetia). The examination of 213 adults collected in Sochi in 2018 showed that 53% were infested with Hesperomyces virescens fungi (Ascomycota: Laboulbeniales) and that 8% were infested with Parasitylenchus bifurcatus nematodes (Nematoda: Tylenchida, Allantonematidae). The examined Ha. axyridis specimens were free of the parasitic mite Coccipolipus hippodamiae. An analysis of the phylogenetic relationships of P. bifurcatus based on 18S rDNA confirmed the morphological identification of this species. Hesperomyces virescens and P. bifurcatus were first recorded in the Caucasus and Russia, although they are rather widespread in Europe. This likely indicates that they appeared as a result of coinvasion with their host because the populations of Ha. axyridis, He. virescens and P. bifurcatus in the Caucasus are isolated from the main parts of the ranges of these species in Europe. The nearest localities of Ha. axyridis is on another shore of the Black Sea, and the nearest localities of He. virescens and P. bifurcatus are more than 1000 km from the Caucasus. It is impossible to determine whether the first founders of the Caucasian population were infested with the parasites or whether the parasites were introduced by specimens of Ha. axyridis that arrived later from Europe. Harmonia axyridis was released in the region for pest control, but laboratory cultures are always free of He. virescens and P. bifurcatus. Therefore, the detection of He. virescens and P. bifurcatus indicates that the population of Ha. axyridis in the Caucasus could not have derived exclusively from released specimens. We
Parasitology, Aug 28, 2013
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Papers by Sergei Spiridonov