Raw data on intraspecific divergence and distance to nearest neighbour for the dataset DS-ZACKPLA... more Raw data on intraspecific divergence and distance to nearest neighbour for the dataset DS-ZACKPLAN (dx.doi.org/10.5883/DS-ZACKPLAN) and the DNA barcode region ITS
Raw data on the intraspecific divergence and distance to nearest neighbour for the dataset DS-ZAC... more Raw data on the intraspecific divergence and distance to nearest neighbour for the dataset DS-ZACKANIM (dx.doi.org/10.5883/DS-ZACKANIM) and the DNA barcode region CO
DNA sequences offer powerful tools for describing the members and interactions of natural communi... more DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community
Literature: Backer, CA & RC Bakhuizen van den Brink. 1968. Flora of Java 3: 35-38. Häkkinen, M & ... more Literature: Backer, CA & RC Bakhuizen van den Brink. 1968. Flora of Java 3: 35-38. Häkkinen, M & H Väre. 2008. Typification and checklist of Musa L. names (Musaceae) with nomenclatural notes. Adansonia, sér. 3, 30(1): 63-112. Teodoro, NG.1915. A preliminary study of Philippine bananas. Philippine Journal of Science 10, section c (Botany): 379-419 + 18 pls. Quisumbing EA. 1919. Studies of Philippine bananas. Philippine Agricultural Review 12: 9-90.
The aluminium and phosphorus of an ectomycorrhizal fungus, Suillus variegatus, grown on a petri p... more The aluminium and phosphorus of an ectomycorrhizal fungus, Suillus variegatus, grown on a petri plate was studied using a spectrometer and scanning transmission electromicroscope with electron dispersion photometer. Higher concentrations of soluble Al in the growth medium resulted in the abundant formation of aluminium polyphosphate granules in the cell walls. Although Al was detoxified by P, it led to decreased growth, because P was bound in inactive form.
Polyploidy and symbiotic Epichloë fungal endophytes are common and heritable characteristics that... more Polyploidy and symbiotic Epichloë fungal endophytes are common and heritable characteristics that can facilitate environmental range expansion in grasses. Here we examined geographic patterns of polyploidy and the frequency of fungal endophyte colonized plants in 29 Festuca rubra L. populations from eight geographic sites across latitudes from Spain to northernmost Finland and Greenland. Ploidy seemed to be positively and negatively correlated with latitude and productivity, respectively. However, the correlations were nonlinear; 84% of the plants were hexaploids (2n = 6x = 42), and the positive correlation between ploidy level and latitude is the result of only four populations skewing the data. In the southernmost end of the gradient 86% of the plants were tetraploids (2n = 4x = 28), whereas in the northernmost end of the gradient one population had only octoploid plants (2n = 8x = 56). Endophytes were detected in 22 out of the 29 populations. Endophyte frequencies varied among geographic sites, and populations and habitats within geographic sites irrespective of ploidy, latitude or productivity. The highest overall endophyte frequencies were found in the southernmost end of the gradient, Spain, where 69% of plants harbored endophytes. In northern Finland, endophytes were detected in 30% of grasses but endophyte frequencies varied among populations from 0% to 75%, being higher in meadows compared to riverbanks. The endophytes were detected in 36%, 30% and 27% of the plants in Faroe Islands, Iceland and Switzerland, respectively. Practically all examined plants collected from southern Finland and Greenland were endophyte-free, whereas in other geographic sites endophyte frequencies were highly variable among populations. Common to all populations with high endophyte frequencies is heavy vertebrate grazing. We propose that the detected endophyte frequencies and ploidy levels mirror past distribution history of F. rubra after the last glaciation period, and local adaptations to past or prevailing selection forces such as vertebrate grazing.
Changes in vegetation, soil organic matter content, soil nutrient concentration, microbial activi... more Changes in vegetation, soil organic matter content, soil nutrient concentration, microbial activity and microbial biomass were studied in Scots pine (Pinus sylvestris) forests on the post-glacial land uplift island of Hailuoto in Finland, along altitudinal transects representing about 1000 years of primary succession. The characteristics of microbial communities in the humus layer were compared both within altitude classes and within TWINSPAN (two-way indicator species analysis) clusters of ®eld layer vegetation. Non-metric multidimensional scaling (NMDS) was employed to reveal gradients in the data. During succession, the vegetation changed from dominance by bryophytes and deciduous dwarf shrubs to evergreen dwarf shrubs and lichens. The thickness of the humus layer and the amount of organic matter in the soil decreased along the succession, which in turn reduced microbial biomass, microbial activity and soil nutrients when calculated on an areal basis. The nutrient concentration of the soil OM (organic matter) showed no successional trend on a concentration basis but the C-toN ratio of organic matter increased with increasing soil age and lichen coverage. Thus, the nutrient availability decreased during succession but this could not be demonstrated by calculating results against unit weight of organic matter. Soil basal respiration and microbial biomass increased during the succession when calculated per unit weight of organic matter. The successional decrease in site productivity appeared to be due to leaching of nutrients from the sandy mineral soil and thinning of the humus layer. Plants and soil microbes became increasingly N limited during the course of the succession, suggesting the increased importance of mycorrhizal symbiosis for plant performance and increased energy costs among soil microbes in nutrient uptake.
Exploring the regional pattern of variation in traits driven by symbiotic interactions may provid... more Exploring the regional pattern of variation in traits driven by symbiotic interactions may provide insights to understand the evolutionary processes that operate over plant populations. Polyploidy, which is associated with fitness improvement, is expected to increase with latitude and altitude. However, it has never been explored in relation with the occurrence of epichloid fungal endophytes in plants. Both, variation in ploidy level and in the incidence of fungal endophytes, are known to occur in species of fine fescues. Here, we surveyed the occurrence of systemic fungal endophytes in natural Festuca vivipara populations in North European islands. In addition, we identified the fungal species associated with this grass and determined the predominant ploidy level for each population. Endophytes were found in four of six, two of three, and one of three populations for Faroe Islands, Iceland and Great Britain, respectively. With an average low incidence level of 15 % in infected populations, there was no relationship between infection level and either latitude or altitude. The phylogenetic analysis based on sequences ITS and the tub2 genes, supports that the endophytic species is Epichloë festucae, the same as in other fine fescues. We found no variation in ploidy level as all the plants were tetraploid (4X) with 28 chromosomes, a pattern which contrasts with the variation reported in previous antecedents. Our results suggest that apart from low and variable benefits of the endophyte to the plants, there would be a complex dynamics between epichloid endophytes and species of the fine fescue complex which merits further studies.
The relationship between fungal biomass in the mycorrhizae measured in terms of glucosamine conte... more The relationship between fungal biomass in the mycorrhizae measured in terms of glucosamine content and the yield of sporophores of mycorrhizal fungi was studied in one fertilized and one unfertilized Ioblolly pine (Pinus taeda) plantation in Mississippi, USA. The correlation between glucosamine and the total biomass of sporophores in the subplots was significant in the fertilized stand (r=0.616, p <0.001). Introduction Sporophore biomass production in ectomycorrhizal fungi has been used as an estimate for changes in the forest ecosystems, although it does not necessarily represent the status of the mycorrhizal symbiosis in the roots. The aim here was to determine the intensity of mycorrhizal involvement in the roots of mature loblolly pines by analyzing the glucosamine (chitin) content of the fine roots. The fungal biomass in the roots was then compared with the sporophore production of the mycorrhizal fungi. Determination of glucosamine has been used earlier in young pine seedl...
Hottentot Fern (Thelypteris pozoi) is a widespread species, although in Europe it is only found i... more Hottentot Fern (Thelypteris pozoi) is a widespread species, although in Europe it is only found in the Macaronesian islands, Spain and France, where it is usually found near the sea. It has been assessed as Least Concern.
Polyploidy and symbiotic Epichloë fungal endophytes are common and heritable characteristics that... more Polyploidy and symbiotic Epichloë fungal endophytes are common and heritable characteristics that can facilitate environmental range expansion in grasses. Here we examined geographic patterns of polyploidy and the frequency of fungal endophyte colonized plants in 29 Festuca rubra L. populations from eight geographic sites across latitudes from Spain to northernmost Finland and Greenland. Ploidy seemed to be positively and negatively correlated with latitude and productivity, respectively. However, the correlations were nonlinear; 84% of the plants were hexaploids (2n = 6x = 42), and the positive correlation between ploidy level and latitude is the result of only four populations skewing the data. In the southernmost end of the gradient 86% of the plants were tetraploids (2n = 4x = 28), whereas in the northernmost end of the gradient one population had only octoploid plants (2n = 8x = 56). Endophytes were detected in 22 out of the 29 populations. Endophyte frequencies varied among geographic sites, and populations and habitats within geographic sites irrespective of ploidy, latitude or productivity. The highest overall endophyte frequencies were found in the southernmost end of the gradient, Spain, where 69% of plants harbored endophytes. In northern Finland, endophytes were detected in 30% of grasses but endophyte frequencies varied among populations from 0% to 75%, being higher in meadows compared to riverbanks. The endophytes were detected in 36%, 30% and 27% of the plants in Faroe Islands, Iceland and Switzerland, respectively. Practically all examined plants collected from southern Finland and Greenland were endophyte-free, whereas in other geographic sites endophyte frequencies were highly variable among populations. Common to all populations with high endophyte frequencies is heavy vertebrate grazing. We propose that the detected endophyte frequencies and ploidy levels mirror past distribution history of F. rubra after the last glaciation period, and local adaptations to past or prevailing selection forces such as vertebrate grazing.
DNA sequences offer powerful tools for describing the members and interactions of natural communi... more DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this paper, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively-studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time, and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologicall...
... Vozzo, JA &amp; Hackskaylo, E. 1971. Inoculation of Pinus caribea with ectomycorrhizal fu... more ... Vozzo, JA &amp; Hackskaylo, E. 1971. Inoculation of Pinus caribea with ectomycorrhizal fungi in Puerto Rico. Forest Sci. 17,239-245. Vare, H. 1989. The mycorrhizal condition of weakened Scots pine samplings grown on ploughed sites in northern Finland. Can. J. For. Res. ...
Forest soil ecology was studied in Fennoscandinavian dry Scots pine forests grazed by reindeer to... more Forest soil ecology was studied in Fennoscandinavian dry Scots pine forests grazed by reindeer to varying extents (ungrazed, lichen-dominated-sites; grazed sites; and bryophyte-dominated sites). We hypothesized that the productivity parameters of the site (i.e., tree growth and soil nutrient concentrations), the vegetation composition, and the microbial activities are directly correlated. Since the productivity of the lichen-dominated ecosystem is low, microbial activities are assumed to be naturally low. Grazing was expected to decrease both the amount of Scots pine fine roots and the soil microbial activities. Several variables on the characteristics of the soil microbial community, Scots pine fine roots, soil nutrients, and tree growth were studied in relation to vegetation composition by using non-metric multidimensional scaling (NMDS). Basal respiration (Bas), metabolic quotient of the microbial community (qCO 2), and pine fine root parameters increased toward the ungrazed, nutrient-poor, lichen-dominated sites, which were grouped at one end of the first axis in the NMDS ordination. Soil nutrient and tree growth parameters and thickness of the humus layer increased toward bryophyte-dominated sites, which were grouped at the other end of the first axis in the ordination. The grazed sites fell between them. These were characterized by lower Bas and qCO 2 values and longer lag, compared to ungrazed lichen-or bryophyte-dominated sites, probably due to decreased carbon input and microclimatic change (the soil without lichen carpet is exposed to direct sunlight and wind). Microbial biomass (Cmic), fungal biomass (ergosterol concentration), and the specific growth rate (µCO 2) were not related to vegetation ordination. The high fine root production is the most plausible explanation for the high microbial activities at nutrient-poor, lichen-dominated sites, which produce qualitatively poor and slowly decomposing litter, as fine roots secrete considerable amounts of organic substances. At bryophyte-dominated sites, the higher soil nutrient concentrations and the higher production of easily decomposable substrates are likely to maintain the microbial activities.
Raw data on intraspecific divergence and distance to nearest neighbour for the dataset DS-ZACKPLA... more Raw data on intraspecific divergence and distance to nearest neighbour for the dataset DS-ZACKPLAN (dx.doi.org/10.5883/DS-ZACKPLAN) and the DNA barcode region ITS
Raw data on the intraspecific divergence and distance to nearest neighbour for the dataset DS-ZAC... more Raw data on the intraspecific divergence and distance to nearest neighbour for the dataset DS-ZACKANIM (dx.doi.org/10.5883/DS-ZACKANIM) and the DNA barcode region CO
DNA sequences offer powerful tools for describing the members and interactions of natural communi... more DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community
Literature: Backer, CA & RC Bakhuizen van den Brink. 1968. Flora of Java 3: 35-38. Häkkinen, M & ... more Literature: Backer, CA & RC Bakhuizen van den Brink. 1968. Flora of Java 3: 35-38. Häkkinen, M & H Väre. 2008. Typification and checklist of Musa L. names (Musaceae) with nomenclatural notes. Adansonia, sér. 3, 30(1): 63-112. Teodoro, NG.1915. A preliminary study of Philippine bananas. Philippine Journal of Science 10, section c (Botany): 379-419 + 18 pls. Quisumbing EA. 1919. Studies of Philippine bananas. Philippine Agricultural Review 12: 9-90.
The aluminium and phosphorus of an ectomycorrhizal fungus, Suillus variegatus, grown on a petri p... more The aluminium and phosphorus of an ectomycorrhizal fungus, Suillus variegatus, grown on a petri plate was studied using a spectrometer and scanning transmission electromicroscope with electron dispersion photometer. Higher concentrations of soluble Al in the growth medium resulted in the abundant formation of aluminium polyphosphate granules in the cell walls. Although Al was detoxified by P, it led to decreased growth, because P was bound in inactive form.
Polyploidy and symbiotic Epichloë fungal endophytes are common and heritable characteristics that... more Polyploidy and symbiotic Epichloë fungal endophytes are common and heritable characteristics that can facilitate environmental range expansion in grasses. Here we examined geographic patterns of polyploidy and the frequency of fungal endophyte colonized plants in 29 Festuca rubra L. populations from eight geographic sites across latitudes from Spain to northernmost Finland and Greenland. Ploidy seemed to be positively and negatively correlated with latitude and productivity, respectively. However, the correlations were nonlinear; 84% of the plants were hexaploids (2n = 6x = 42), and the positive correlation between ploidy level and latitude is the result of only four populations skewing the data. In the southernmost end of the gradient 86% of the plants were tetraploids (2n = 4x = 28), whereas in the northernmost end of the gradient one population had only octoploid plants (2n = 8x = 56). Endophytes were detected in 22 out of the 29 populations. Endophyte frequencies varied among geographic sites, and populations and habitats within geographic sites irrespective of ploidy, latitude or productivity. The highest overall endophyte frequencies were found in the southernmost end of the gradient, Spain, where 69% of plants harbored endophytes. In northern Finland, endophytes were detected in 30% of grasses but endophyte frequencies varied among populations from 0% to 75%, being higher in meadows compared to riverbanks. The endophytes were detected in 36%, 30% and 27% of the plants in Faroe Islands, Iceland and Switzerland, respectively. Practically all examined plants collected from southern Finland and Greenland were endophyte-free, whereas in other geographic sites endophyte frequencies were highly variable among populations. Common to all populations with high endophyte frequencies is heavy vertebrate grazing. We propose that the detected endophyte frequencies and ploidy levels mirror past distribution history of F. rubra after the last glaciation period, and local adaptations to past or prevailing selection forces such as vertebrate grazing.
Changes in vegetation, soil organic matter content, soil nutrient concentration, microbial activi... more Changes in vegetation, soil organic matter content, soil nutrient concentration, microbial activity and microbial biomass were studied in Scots pine (Pinus sylvestris) forests on the post-glacial land uplift island of Hailuoto in Finland, along altitudinal transects representing about 1000 years of primary succession. The characteristics of microbial communities in the humus layer were compared both within altitude classes and within TWINSPAN (two-way indicator species analysis) clusters of ®eld layer vegetation. Non-metric multidimensional scaling (NMDS) was employed to reveal gradients in the data. During succession, the vegetation changed from dominance by bryophytes and deciduous dwarf shrubs to evergreen dwarf shrubs and lichens. The thickness of the humus layer and the amount of organic matter in the soil decreased along the succession, which in turn reduced microbial biomass, microbial activity and soil nutrients when calculated on an areal basis. The nutrient concentration of the soil OM (organic matter) showed no successional trend on a concentration basis but the C-toN ratio of organic matter increased with increasing soil age and lichen coverage. Thus, the nutrient availability decreased during succession but this could not be demonstrated by calculating results against unit weight of organic matter. Soil basal respiration and microbial biomass increased during the succession when calculated per unit weight of organic matter. The successional decrease in site productivity appeared to be due to leaching of nutrients from the sandy mineral soil and thinning of the humus layer. Plants and soil microbes became increasingly N limited during the course of the succession, suggesting the increased importance of mycorrhizal symbiosis for plant performance and increased energy costs among soil microbes in nutrient uptake.
Exploring the regional pattern of variation in traits driven by symbiotic interactions may provid... more Exploring the regional pattern of variation in traits driven by symbiotic interactions may provide insights to understand the evolutionary processes that operate over plant populations. Polyploidy, which is associated with fitness improvement, is expected to increase with latitude and altitude. However, it has never been explored in relation with the occurrence of epichloid fungal endophytes in plants. Both, variation in ploidy level and in the incidence of fungal endophytes, are known to occur in species of fine fescues. Here, we surveyed the occurrence of systemic fungal endophytes in natural Festuca vivipara populations in North European islands. In addition, we identified the fungal species associated with this grass and determined the predominant ploidy level for each population. Endophytes were found in four of six, two of three, and one of three populations for Faroe Islands, Iceland and Great Britain, respectively. With an average low incidence level of 15 % in infected populations, there was no relationship between infection level and either latitude or altitude. The phylogenetic analysis based on sequences ITS and the tub2 genes, supports that the endophytic species is Epichloë festucae, the same as in other fine fescues. We found no variation in ploidy level as all the plants were tetraploid (4X) with 28 chromosomes, a pattern which contrasts with the variation reported in previous antecedents. Our results suggest that apart from low and variable benefits of the endophyte to the plants, there would be a complex dynamics between epichloid endophytes and species of the fine fescue complex which merits further studies.
The relationship between fungal biomass in the mycorrhizae measured in terms of glucosamine conte... more The relationship between fungal biomass in the mycorrhizae measured in terms of glucosamine content and the yield of sporophores of mycorrhizal fungi was studied in one fertilized and one unfertilized Ioblolly pine (Pinus taeda) plantation in Mississippi, USA. The correlation between glucosamine and the total biomass of sporophores in the subplots was significant in the fertilized stand (r=0.616, p <0.001). Introduction Sporophore biomass production in ectomycorrhizal fungi has been used as an estimate for changes in the forest ecosystems, although it does not necessarily represent the status of the mycorrhizal symbiosis in the roots. The aim here was to determine the intensity of mycorrhizal involvement in the roots of mature loblolly pines by analyzing the glucosamine (chitin) content of the fine roots. The fungal biomass in the roots was then compared with the sporophore production of the mycorrhizal fungi. Determination of glucosamine has been used earlier in young pine seedl...
Hottentot Fern (Thelypteris pozoi) is a widespread species, although in Europe it is only found i... more Hottentot Fern (Thelypteris pozoi) is a widespread species, although in Europe it is only found in the Macaronesian islands, Spain and France, where it is usually found near the sea. It has been assessed as Least Concern.
Polyploidy and symbiotic Epichloë fungal endophytes are common and heritable characteristics that... more Polyploidy and symbiotic Epichloë fungal endophytes are common and heritable characteristics that can facilitate environmental range expansion in grasses. Here we examined geographic patterns of polyploidy and the frequency of fungal endophyte colonized plants in 29 Festuca rubra L. populations from eight geographic sites across latitudes from Spain to northernmost Finland and Greenland. Ploidy seemed to be positively and negatively correlated with latitude and productivity, respectively. However, the correlations were nonlinear; 84% of the plants were hexaploids (2n = 6x = 42), and the positive correlation between ploidy level and latitude is the result of only four populations skewing the data. In the southernmost end of the gradient 86% of the plants were tetraploids (2n = 4x = 28), whereas in the northernmost end of the gradient one population had only octoploid plants (2n = 8x = 56). Endophytes were detected in 22 out of the 29 populations. Endophyte frequencies varied among geographic sites, and populations and habitats within geographic sites irrespective of ploidy, latitude or productivity. The highest overall endophyte frequencies were found in the southernmost end of the gradient, Spain, where 69% of plants harbored endophytes. In northern Finland, endophytes were detected in 30% of grasses but endophyte frequencies varied among populations from 0% to 75%, being higher in meadows compared to riverbanks. The endophytes were detected in 36%, 30% and 27% of the plants in Faroe Islands, Iceland and Switzerland, respectively. Practically all examined plants collected from southern Finland and Greenland were endophyte-free, whereas in other geographic sites endophyte frequencies were highly variable among populations. Common to all populations with high endophyte frequencies is heavy vertebrate grazing. We propose that the detected endophyte frequencies and ploidy levels mirror past distribution history of F. rubra after the last glaciation period, and local adaptations to past or prevailing selection forces such as vertebrate grazing.
DNA sequences offer powerful tools for describing the members and interactions of natural communi... more DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this paper, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively-studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time, and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologicall...
... Vozzo, JA &amp; Hackskaylo, E. 1971. Inoculation of Pinus caribea with ectomycorrhizal fu... more ... Vozzo, JA &amp; Hackskaylo, E. 1971. Inoculation of Pinus caribea with ectomycorrhizal fungi in Puerto Rico. Forest Sci. 17,239-245. Vare, H. 1989. The mycorrhizal condition of weakened Scots pine samplings grown on ploughed sites in northern Finland. Can. J. For. Res. ...
Forest soil ecology was studied in Fennoscandinavian dry Scots pine forests grazed by reindeer to... more Forest soil ecology was studied in Fennoscandinavian dry Scots pine forests grazed by reindeer to varying extents (ungrazed, lichen-dominated-sites; grazed sites; and bryophyte-dominated sites). We hypothesized that the productivity parameters of the site (i.e., tree growth and soil nutrient concentrations), the vegetation composition, and the microbial activities are directly correlated. Since the productivity of the lichen-dominated ecosystem is low, microbial activities are assumed to be naturally low. Grazing was expected to decrease both the amount of Scots pine fine roots and the soil microbial activities. Several variables on the characteristics of the soil microbial community, Scots pine fine roots, soil nutrients, and tree growth were studied in relation to vegetation composition by using non-metric multidimensional scaling (NMDS). Basal respiration (Bas), metabolic quotient of the microbial community (qCO 2), and pine fine root parameters increased toward the ungrazed, nutrient-poor, lichen-dominated sites, which were grouped at one end of the first axis in the NMDS ordination. Soil nutrient and tree growth parameters and thickness of the humus layer increased toward bryophyte-dominated sites, which were grouped at the other end of the first axis in the ordination. The grazed sites fell between them. These were characterized by lower Bas and qCO 2 values and longer lag, compared to ungrazed lichen-or bryophyte-dominated sites, probably due to decreased carbon input and microclimatic change (the soil without lichen carpet is exposed to direct sunlight and wind). Microbial biomass (Cmic), fungal biomass (ergosterol concentration), and the specific growth rate (µCO 2) were not related to vegetation ordination. The high fine root production is the most plausible explanation for the high microbial activities at nutrient-poor, lichen-dominated sites, which produce qualitatively poor and slowly decomposing litter, as fine roots secrete considerable amounts of organic substances. At bryophyte-dominated sites, the higher soil nutrient concentrations and the higher production of easily decomposable substrates are likely to maintain the microbial activities.
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Papers by H. Väre