Self-targetability is an emerging targeting strategy for polymer nanocarriers with facile prepara... more Self-targetability is an emerging targeting strategy for polymer nanocarriers with facile preparation and high targeting efficiency. An acid-sensitive dextran-doxorubicin prodrug (Dex-g-DOX) has been synthesized and used as a self-targeted drug delivery system for the treatment of orthotopic hepatoma. The polysaccharide prodrug exhibits ultraselective accumulation in cancerous liver tissue, acid-sensitive DOX release within cells, and high antitumor efficacy in vitro and in vivo. Therefore, Dex-g-DOX demonstrates great potential for chemotherapy of orthotopic hepatoma.
Recently, chemotherapy has been one of the most important therapeutic approaches for malignant tu... more Recently, chemotherapy has been one of the most important therapeutic approaches for malignant tumors. The tumor tissular or intracellular microenvironment-sensitive polymer-doxorubicin (DOX) conjugates demonstrate great potential for improved antitumor efficacy and reduced side effects. In this work, the acid-sensitive dextran-DOX conjugate (noted as Dex-O-DOX) was synthesized through the versatile efficient oximation reaction between the terminal aldehyde group of polysaccharide and the amino group in DOX in the buffer solution of sodium acetate/acetic acid. The insensitive one, i.e., Dex-b-DOX, was prepared similarly as Dex-O-DOX with a supplemented reduction reaction. The DOX release from Dex-O-DOX was pH-dependent and accelerated by the decreased pH. The efficient intracellular DOX release from Dex-O-DOX toward the human hepatoma HepG2 cells was further confirmed. Furthermore, Dex-O-DOX exhibited a closer antiproliferative activity to free DOX·HCl as the extension of time. More...
Poly(L-glutamic acid) (PLG) and its derivatives, which are biodegradable and biocompatible, are o... more Poly(L-glutamic acid) (PLG) and its derivatives, which are biodegradable and biocompatible, are one kind of the most widely investigated synthetic polypeptides as biomedical materials benefited from their pH-responsive property (pK a ～ 4.1) and modifiable side carboxyl group. In this work, PLG was synthesized via a two-step procedure. Poly(γ-benzyl-L-glutamate) (PBLG) was first synthesized by the ring-opening polymerization of γ-benzyl-L-glutamate-N-carboxyanhydride (BLG-NCA) using n-hexylamine as initiator, and then PLG was prepared by the deprotection of benzyl groups in PBLG. PLG microsphere was prepared by the oil-in-oil (O/O) anhydrous emulsion method utilizing N,N-dimetylformamide as an inner oil phase and corn oil as an outer oil phase. Rifampicin, a model drug, was loaded into the PLG microsphere, and the drug loading capacity and entrapment efficiency were revealed. The scanning electron microscopy micrograph indicated that the drug-loaded microsphere exhibited spherical morphology with narrow size distribution and average diameter at about 9.0 μm. To investigate the application of PLG microsphere in oral drug delivery (especially for enteric-coated drugs), the in vitro rifampicin release experiments in simulated gastric or intestinal fluid were performed. In vitro release results revealed that the release of rifampicin from microsphere was highly dependent on pH. In detail, less amount of rifampicin was released in simulated gastric fluid, while the quicker release of rifampicin occurred in simulated intestinal fluid. It was because that the deprotonation of carboxyl groups in PLG caused the loose, expansion, and even disintegration of PLG microsphere in simulated intestinal fluid. Moreover, the morphology of microsphere in simulated gastric fluid was different from that in simulated intestinal fluid, which further confirmed the pH-sensitive property of microsphere. Additionally, in vitro methyl thiazolyl tetrazolium assays demonstrated that the PLG microsphere was biocompatible. Therefore, the biocompatible PLG microsphere with the intelligent pH-triggered drug release should be promising for application in oral drug delivery. 通过 MTT 法以 SDS 作为阳性对照检测聚氨基酸微 球对 HeLa 细胞的毒性. 在 96 孔板中按照 7000 细胞/ 孔的密度种植 HeLa 细胞, 并在每个孔内加入 200 μL 的 DMEM 培养基. DMEM 培养基的主要组成为 10%牛血 清, 50 IU•mL -1 的青霉素和 50 IU•mL -1 的链霉素. 将细 胞培养 24 h, 细胞培养的外部环境为 37 ℃, 二氧化碳 (CO 2 )的含量为 5% (V/V). 当细胞培养 24 h 后将培养液 移除并在每个孔中加入 200 μL 含空白 PLG 微球的新鲜 培养液, 空白 PLG 微球的浓度范围为 0～0.1 mg•mL -1 . 将细胞与载体材料共培养 72 h 后向每个孔中加入 20.0 μL MTT 原液(5.0 mg•mL -1 , PBS 溶液). 将细胞继续培养 4 h 后进行 MTT 测试. 仪器为 Bio-Rad 680 酶标仪, 吸光 度检测波长为 490 nm. 细胞存活率通过下列公式计算: Cell Viability (%)＝(A 样品/A 对照)×100 其中, A 样品和 A 对照分别表示样品组和空白组的吸光度.
cancer therapy. Moreover, since CB[8] is able to simultaneously bind two guests, it provides us w... more cancer therapy. Moreover, since CB[8] is able to simultaneously bind two guests, it provides us with an opportunity to integrate different functional groups into ternary complexes which could be used as a versatile approach for preparation of multifunctional nanocarriers.
The reduction-responsive polymeric nanocarriers have attracted considerable interest because of a... more The reduction-responsive polymeric nanocarriers have attracted considerable interest because of a significantly higher concentration of intracellular glutathione in comparison with that outside cells. The smart nanovehicles can selectively transport the antitumor drugs into cells to improve efficacies and decrease side effects. In this work, a facilely prepared glutathione-degradable nanogel was employed for targeting intracellular delivery of an antitumor drug (ie, doxorubicin [DOX]). DOX was loaded into nanogel through a sequential dispersion and dialysis approach with a drug loading efficiency of 56.8 wt%, and the laden nanogel (noted as NG/DOX) showed an appropriate hydrodynamic radius of 56.1±3.5 nm. NG/DOX exhibited enhanced or improved maximum tolerated dose on healthy Kunming mice and enhanced intratumoral accumulation and dose-dependent antitumor efficacy toward H22 hepatoma-xenografted mouse model compared with free drug. In addition, the upregulated antitumor efficacy of ...
Apart from the immunosuppressive property, which has been widely investigated in the treatment of... more Apart from the immunosuppressive property, which has been widely investigated in the treatment of various autoimmune diseases, bone marrow mesenchymal stem cells (BMMSCs) also exhibit the chondrogenic capacity. Recently, BMMSCs have attracted more and more attention in the remission of rheumatoid arthritis (RA) and the reconstruction of cartilage injury. In addition to the significant regulatory hurdles of systemic treatment by BMMSCs, the poor inhibitory efficiency on articular inflammatory reaction and the inferior result of preventing the persistent destruction of cartilage were observed. Herein, toward the ovalbumin (OVA)-induced arthritis in rabbits, the in situ transplantation of fibrin gel-encapsulated BMMSCs to osteochondral defect was confirmed to result in the decreased levels of cytokines, such as interleukin-1β, interleukin-6, tumor necrosis factor-α, and anti-OVA antibody, in the serum. What is more, the implantation of BMMSCs also inhibited the proliferation of antigen...
2008 International Conference on Microwave and Millimeter Wave Technology, 2008
A new multiresolution (MR) preconditioner is presented in this paper, and it is combined with the... more A new multiresolution (MR) preconditioner is presented in this paper, and it is combined with the multilevel fast multipole algorithm (MLFMA) for the analysis of electromagnetic scatters; Furthermore, the MR preconditioner is modified to be more effective by including a perturbation which is constructed from the principle value term of the magnetic field integral equation (MFIE) operator for solving the
Proceedings Third International Symposium on High-Performance Computer Architecture, 1997
Copyright 1997 IEEE. Published in the Proceedings of the Third International Symposium on High Pe... more Copyright 1997 IEEE. Published in the Proceedings of the Third International Symposium on High Performance Computer Architecture, February 1-5, 1997 in San Antonio, Texas, USA. Personal use of this material is permitted. However, permission to reprint/republish this material for ...
Self-healing polymeric hydrogels have the capability to recover their structures and functionalit... more Self-healing polymeric hydrogels have the capability to recover their structures and functionalities upon injury, which are extremely attractive in emerging biomedical applications. This research reports a new kind of self-healing polypeptide hydrogels based on self-assembly between cholesterol (Chol)-modified triblock poly(l-glutamic acid)-block-poly(ethylene glycol)-block-poly(l-glutamic acid) ((PLGA-b-PEG-b-PLGA)-g-Chol) and β-cyclodextrin (β-CD)-modified poly(l-glutamic acid) (PLGA-g-β-CD). The hydrogel formation relied on the host and guest linkage between β-CD and Chol. This study demonstrates the influences of polymer concentration and β-CD/Chol molar ratio on viscoelastic behavior of the hydrogels. The results showed that storage modulus was highest at polymer concentration of 15% w/v and β-CD/Chol molar ratio of 1:1. The effect of the PLGA molecular weight in (PLGA-b-PEG-b-PLGA)-g-Chol on viscoelastic behavior, mechanical properties and in vitro degradation of the supramole...
ABSTRACT Complications arising from tendon injury include tendon sheath infection and peritendino... more ABSTRACT Complications arising from tendon injury include tendon sheath infection and peritendinous adhesion, in which tendon adhesion often leads to serious motor dysfunction. In this work, the electrospun membranes of poly(L-lactide) (PLA) and poly(ε-caprolactone) (PCL) with different degradation kinetics were used to investigate their efficacy for anti-adhesion toward Achilles tendon repair. Compared with the PCL membrane, the PLA sample showed a faster rate of degradation in 42 d, and all the degradation media (i.e., phosphate-buffered saline) maintained at a constant pH of around 7.4. Meanwhile, the superior biocompatibility of both the PLA and PCL membranes were proved by the in vitro cellular adhesion tests and in vivo histopathological assays. Simultaneously, the PLA membrane was more effective than the PCL sample in decreasing adhesion and promoting functional recovery. Furthermore, the experiment result was further confirmed by hematoxylin-eosin and Masson’s trichrome staining, and type I collagen immunohistochemical analysis. All results revealed that the model treated with the electrospun PLA membrane was obviously better with regard to both anti-adhesion and tendon repair than that in the PCL membrane group. Considering the results of degradation and adhesion prevention efficacy, the electrospun polyester membranes, especially the PLA one, would be applied with fascinating potential in clinical prevention of postoperative tendon adhesion.
Benefited from the high orientation of coordinated interaction, metallo-supramolecular materials ... more Benefited from the high orientation of coordinated interaction, metallo-supramolecular materials have attracted enormous interests in many fields. Herein, a novel metallo-supramolecular nanogel (SNG)-based drug delivery system for synergistic chemo-photodynamic therapy is explored to enhance anticancer efficacy. It is fabricated by the metallo-supramolecular-coordinated interaction between tetraphenylporphyrin zinc (Zn-Por) and histidine. It can respond to tumor acid microenvironment to release the co-delivered anticancer drug and photosensitizer to kill the lesion cells. Zn-Por moieties in SNG keep the photosensitivity in the range of visible wavelength and possess the ability of generating active oxygen species for photodynamic therapy. The drug-loaded SNG provides a di-functional platform for chemotherapy and photodynamic therapy. Compared with the single chemotherapy of free doxorubicine (DOX) or photodynamic therapy of Zn-Por in SNG, DOX-loaded SNG with irradiation shows higher...
The profound effect of annealing on the electrospun poly(3-caprolactone) scaffold is comprehensiv... more The profound effect of annealing on the electrospun poly(3-caprolactone) scaffold is comprehensively investigated for the first time.
Electrospun biodegradable polymer membranes can effectively serve as barriers to prevent postoper... more Electrospun biodegradable polymer membranes can effectively serve as barriers to prevent postoperative intestinal adhesion. Previous studies have largely focused on utilizing different electrospun variables to regulate membrane properties, but paid very limited attention to the straightforward influences of raw material characteristics. In the present work, the physical and physiological properties of electrospun poly(3-caprolactone) (PCL) membranes with varying viscosity-average molecular weights (M h ¼ 40 000, 80 000 and 120 000 g mol À1 ) were explored for the first time. Interestingly, the typical properties of electrospun films, such as the morphological structure, mechanical properties, degradation kinetics and the anti-adhesion effect, were revealed to be substantially dependent on the molecular weight. In clear contrast, the PCL sample with the viscosity-average molecular weight of 80 000 g mol À1 exhibited the best performance, including a regular fibrous morphology, superior tensile strength and Young's modulus of 1.13 and 8.41 MPa, respectively, which was presumably ascribed to the means of chain entanglements and interactions. Most importantly, no cytotoxicity was traced in the electrospun PCL membranes as revealed from the cell culture test; moreover, a significant reduction of postoperative adhesion was observed. Because of the above mentioned excellent merits, the electrospun PCL membranes can be regarded as excellent candidates for the anti-adhesion applications.
A kind of glucose-sensitive polypeptide nanogel was prepared via a two-step procedure. First, met... more A kind of glucose-sensitive polypeptide nanogel was prepared via a two-step procedure. First, methoxy poly(ethylene glycol)-block-poly(γ-benzyl-L-glutamate-co-(γ-propargyl-L-glutamate-graft-glucose) (mPEG-b-P(BLG-co-(PLG-g-Glu))) was synthesized by clicking 2'-azidoethyl-O-α-D-glucopyranoside to the PLG unit in mPEG-b-P(BLG-co-PLG), which was synthesized by the ring-opening polymerization (ROP) of γ-benzyl-L-glutamate N-carboxyanhydride and γ-propargyl-L-glutamate N-carboxyanhydride with mPEG-NH 2 as the macroinitiator. The novel nanogel was subsequently prepared by cross-linking the glucose moieties through adipoylamidophenylboronic acid (AAPBA). The formation of the nanogel, i.e.
Self-targetability is an emerging targeting strategy for polymer nanocarriers with facile prepara... more Self-targetability is an emerging targeting strategy for polymer nanocarriers with facile preparation and high targeting efficiency. An acid-sensitive dextran-doxorubicin prodrug (Dex-g-DOX) has been synthesized and used as a self-targeted drug delivery system for the treatment of orthotopic hepatoma. The polysaccharide prodrug exhibits ultraselective accumulation in cancerous liver tissue, acid-sensitive DOX release within cells, and high antitumor efficacy in vitro and in vivo. Therefore, Dex-g-DOX demonstrates great potential for chemotherapy of orthotopic hepatoma.
Recently, chemotherapy has been one of the most important therapeutic approaches for malignant tu... more Recently, chemotherapy has been one of the most important therapeutic approaches for malignant tumors. The tumor tissular or intracellular microenvironment-sensitive polymer-doxorubicin (DOX) conjugates demonstrate great potential for improved antitumor efficacy and reduced side effects. In this work, the acid-sensitive dextran-DOX conjugate (noted as Dex-O-DOX) was synthesized through the versatile efficient oximation reaction between the terminal aldehyde group of polysaccharide and the amino group in DOX in the buffer solution of sodium acetate/acetic acid. The insensitive one, i.e., Dex-b-DOX, was prepared similarly as Dex-O-DOX with a supplemented reduction reaction. The DOX release from Dex-O-DOX was pH-dependent and accelerated by the decreased pH. The efficient intracellular DOX release from Dex-O-DOX toward the human hepatoma HepG2 cells was further confirmed. Furthermore, Dex-O-DOX exhibited a closer antiproliferative activity to free DOX·HCl as the extension of time. More...
Poly(L-glutamic acid) (PLG) and its derivatives, which are biodegradable and biocompatible, are o... more Poly(L-glutamic acid) (PLG) and its derivatives, which are biodegradable and biocompatible, are one kind of the most widely investigated synthetic polypeptides as biomedical materials benefited from their pH-responsive property (pK a ～ 4.1) and modifiable side carboxyl group. In this work, PLG was synthesized via a two-step procedure. Poly(γ-benzyl-L-glutamate) (PBLG) was first synthesized by the ring-opening polymerization of γ-benzyl-L-glutamate-N-carboxyanhydride (BLG-NCA) using n-hexylamine as initiator, and then PLG was prepared by the deprotection of benzyl groups in PBLG. PLG microsphere was prepared by the oil-in-oil (O/O) anhydrous emulsion method utilizing N,N-dimetylformamide as an inner oil phase and corn oil as an outer oil phase. Rifampicin, a model drug, was loaded into the PLG microsphere, and the drug loading capacity and entrapment efficiency were revealed. The scanning electron microscopy micrograph indicated that the drug-loaded microsphere exhibited spherical morphology with narrow size distribution and average diameter at about 9.0 μm. To investigate the application of PLG microsphere in oral drug delivery (especially for enteric-coated drugs), the in vitro rifampicin release experiments in simulated gastric or intestinal fluid were performed. In vitro release results revealed that the release of rifampicin from microsphere was highly dependent on pH. In detail, less amount of rifampicin was released in simulated gastric fluid, while the quicker release of rifampicin occurred in simulated intestinal fluid. It was because that the deprotonation of carboxyl groups in PLG caused the loose, expansion, and even disintegration of PLG microsphere in simulated intestinal fluid. Moreover, the morphology of microsphere in simulated gastric fluid was different from that in simulated intestinal fluid, which further confirmed the pH-sensitive property of microsphere. Additionally, in vitro methyl thiazolyl tetrazolium assays demonstrated that the PLG microsphere was biocompatible. Therefore, the biocompatible PLG microsphere with the intelligent pH-triggered drug release should be promising for application in oral drug delivery. 通过 MTT 法以 SDS 作为阳性对照检测聚氨基酸微 球对 HeLa 细胞的毒性. 在 96 孔板中按照 7000 细胞/ 孔的密度种植 HeLa 细胞, 并在每个孔内加入 200 μL 的 DMEM 培养基. DMEM 培养基的主要组成为 10%牛血 清, 50 IU•mL -1 的青霉素和 50 IU•mL -1 的链霉素. 将细 胞培养 24 h, 细胞培养的外部环境为 37 ℃, 二氧化碳 (CO 2 )的含量为 5% (V/V). 当细胞培养 24 h 后将培养液 移除并在每个孔中加入 200 μL 含空白 PLG 微球的新鲜 培养液, 空白 PLG 微球的浓度范围为 0～0.1 mg•mL -1 . 将细胞与载体材料共培养 72 h 后向每个孔中加入 20.0 μL MTT 原液(5.0 mg•mL -1 , PBS 溶液). 将细胞继续培养 4 h 后进行 MTT 测试. 仪器为 Bio-Rad 680 酶标仪, 吸光 度检测波长为 490 nm. 细胞存活率通过下列公式计算: Cell Viability (%)＝(A 样品/A 对照)×100 其中, A 样品和 A 对照分别表示样品组和空白组的吸光度.
cancer therapy. Moreover, since CB[8] is able to simultaneously bind two guests, it provides us w... more cancer therapy. Moreover, since CB[8] is able to simultaneously bind two guests, it provides us with an opportunity to integrate different functional groups into ternary complexes which could be used as a versatile approach for preparation of multifunctional nanocarriers.
The reduction-responsive polymeric nanocarriers have attracted considerable interest because of a... more The reduction-responsive polymeric nanocarriers have attracted considerable interest because of a significantly higher concentration of intracellular glutathione in comparison with that outside cells. The smart nanovehicles can selectively transport the antitumor drugs into cells to improve efficacies and decrease side effects. In this work, a facilely prepared glutathione-degradable nanogel was employed for targeting intracellular delivery of an antitumor drug (ie, doxorubicin [DOX]). DOX was loaded into nanogel through a sequential dispersion and dialysis approach with a drug loading efficiency of 56.8 wt%, and the laden nanogel (noted as NG/DOX) showed an appropriate hydrodynamic radius of 56.1±3.5 nm. NG/DOX exhibited enhanced or improved maximum tolerated dose on healthy Kunming mice and enhanced intratumoral accumulation and dose-dependent antitumor efficacy toward H22 hepatoma-xenografted mouse model compared with free drug. In addition, the upregulated antitumor efficacy of ...
Apart from the immunosuppressive property, which has been widely investigated in the treatment of... more Apart from the immunosuppressive property, which has been widely investigated in the treatment of various autoimmune diseases, bone marrow mesenchymal stem cells (BMMSCs) also exhibit the chondrogenic capacity. Recently, BMMSCs have attracted more and more attention in the remission of rheumatoid arthritis (RA) and the reconstruction of cartilage injury. In addition to the significant regulatory hurdles of systemic treatment by BMMSCs, the poor inhibitory efficiency on articular inflammatory reaction and the inferior result of preventing the persistent destruction of cartilage were observed. Herein, toward the ovalbumin (OVA)-induced arthritis in rabbits, the in situ transplantation of fibrin gel-encapsulated BMMSCs to osteochondral defect was confirmed to result in the decreased levels of cytokines, such as interleukin-1β, interleukin-6, tumor necrosis factor-α, and anti-OVA antibody, in the serum. What is more, the implantation of BMMSCs also inhibited the proliferation of antigen...
2008 International Conference on Microwave and Millimeter Wave Technology, 2008
A new multiresolution (MR) preconditioner is presented in this paper, and it is combined with the... more A new multiresolution (MR) preconditioner is presented in this paper, and it is combined with the multilevel fast multipole algorithm (MLFMA) for the analysis of electromagnetic scatters; Furthermore, the MR preconditioner is modified to be more effective by including a perturbation which is constructed from the principle value term of the magnetic field integral equation (MFIE) operator for solving the
Proceedings Third International Symposium on High-Performance Computer Architecture, 1997
Copyright 1997 IEEE. Published in the Proceedings of the Third International Symposium on High Pe... more Copyright 1997 IEEE. Published in the Proceedings of the Third International Symposium on High Performance Computer Architecture, February 1-5, 1997 in San Antonio, Texas, USA. Personal use of this material is permitted. However, permission to reprint/republish this material for ...
Self-healing polymeric hydrogels have the capability to recover their structures and functionalit... more Self-healing polymeric hydrogels have the capability to recover their structures and functionalities upon injury, which are extremely attractive in emerging biomedical applications. This research reports a new kind of self-healing polypeptide hydrogels based on self-assembly between cholesterol (Chol)-modified triblock poly(l-glutamic acid)-block-poly(ethylene glycol)-block-poly(l-glutamic acid) ((PLGA-b-PEG-b-PLGA)-g-Chol) and β-cyclodextrin (β-CD)-modified poly(l-glutamic acid) (PLGA-g-β-CD). The hydrogel formation relied on the host and guest linkage between β-CD and Chol. This study demonstrates the influences of polymer concentration and β-CD/Chol molar ratio on viscoelastic behavior of the hydrogels. The results showed that storage modulus was highest at polymer concentration of 15% w/v and β-CD/Chol molar ratio of 1:1. The effect of the PLGA molecular weight in (PLGA-b-PEG-b-PLGA)-g-Chol on viscoelastic behavior, mechanical properties and in vitro degradation of the supramole...
ABSTRACT Complications arising from tendon injury include tendon sheath infection and peritendino... more ABSTRACT Complications arising from tendon injury include tendon sheath infection and peritendinous adhesion, in which tendon adhesion often leads to serious motor dysfunction. In this work, the electrospun membranes of poly(L-lactide) (PLA) and poly(ε-caprolactone) (PCL) with different degradation kinetics were used to investigate their efficacy for anti-adhesion toward Achilles tendon repair. Compared with the PCL membrane, the PLA sample showed a faster rate of degradation in 42 d, and all the degradation media (i.e., phosphate-buffered saline) maintained at a constant pH of around 7.4. Meanwhile, the superior biocompatibility of both the PLA and PCL membranes were proved by the in vitro cellular adhesion tests and in vivo histopathological assays. Simultaneously, the PLA membrane was more effective than the PCL sample in decreasing adhesion and promoting functional recovery. Furthermore, the experiment result was further confirmed by hematoxylin-eosin and Masson’s trichrome staining, and type I collagen immunohistochemical analysis. All results revealed that the model treated with the electrospun PLA membrane was obviously better with regard to both anti-adhesion and tendon repair than that in the PCL membrane group. Considering the results of degradation and adhesion prevention efficacy, the electrospun polyester membranes, especially the PLA one, would be applied with fascinating potential in clinical prevention of postoperative tendon adhesion.
Benefited from the high orientation of coordinated interaction, metallo-supramolecular materials ... more Benefited from the high orientation of coordinated interaction, metallo-supramolecular materials have attracted enormous interests in many fields. Herein, a novel metallo-supramolecular nanogel (SNG)-based drug delivery system for synergistic chemo-photodynamic therapy is explored to enhance anticancer efficacy. It is fabricated by the metallo-supramolecular-coordinated interaction between tetraphenylporphyrin zinc (Zn-Por) and histidine. It can respond to tumor acid microenvironment to release the co-delivered anticancer drug and photosensitizer to kill the lesion cells. Zn-Por moieties in SNG keep the photosensitivity in the range of visible wavelength and possess the ability of generating active oxygen species for photodynamic therapy. The drug-loaded SNG provides a di-functional platform for chemotherapy and photodynamic therapy. Compared with the single chemotherapy of free doxorubicine (DOX) or photodynamic therapy of Zn-Por in SNG, DOX-loaded SNG with irradiation shows higher...
The profound effect of annealing on the electrospun poly(3-caprolactone) scaffold is comprehensiv... more The profound effect of annealing on the electrospun poly(3-caprolactone) scaffold is comprehensively investigated for the first time.
Electrospun biodegradable polymer membranes can effectively serve as barriers to prevent postoper... more Electrospun biodegradable polymer membranes can effectively serve as barriers to prevent postoperative intestinal adhesion. Previous studies have largely focused on utilizing different electrospun variables to regulate membrane properties, but paid very limited attention to the straightforward influences of raw material characteristics. In the present work, the physical and physiological properties of electrospun poly(3-caprolactone) (PCL) membranes with varying viscosity-average molecular weights (M h ¼ 40 000, 80 000 and 120 000 g mol À1 ) were explored for the first time. Interestingly, the typical properties of electrospun films, such as the morphological structure, mechanical properties, degradation kinetics and the anti-adhesion effect, were revealed to be substantially dependent on the molecular weight. In clear contrast, the PCL sample with the viscosity-average molecular weight of 80 000 g mol À1 exhibited the best performance, including a regular fibrous morphology, superior tensile strength and Young's modulus of 1.13 and 8.41 MPa, respectively, which was presumably ascribed to the means of chain entanglements and interactions. Most importantly, no cytotoxicity was traced in the electrospun PCL membranes as revealed from the cell culture test; moreover, a significant reduction of postoperative adhesion was observed. Because of the above mentioned excellent merits, the electrospun PCL membranes can be regarded as excellent candidates for the anti-adhesion applications.
A kind of glucose-sensitive polypeptide nanogel was prepared via a two-step procedure. First, met... more A kind of glucose-sensitive polypeptide nanogel was prepared via a two-step procedure. First, methoxy poly(ethylene glycol)-block-poly(γ-benzyl-L-glutamate-co-(γ-propargyl-L-glutamate-graft-glucose) (mPEG-b-P(BLG-co-(PLG-g-Glu))) was synthesized by clicking 2'-azidoethyl-O-α-D-glucopyranoside to the PLG unit in mPEG-b-P(BLG-co-PLG), which was synthesized by the ring-opening polymerization (ROP) of γ-benzyl-L-glutamate N-carboxyanhydride and γ-propargyl-L-glutamate N-carboxyanhydride with mPEG-NH 2 as the macroinitiator. The novel nanogel was subsequently prepared by cross-linking the glucose moieties through adipoylamidophenylboronic acid (AAPBA). The formation of the nanogel, i.e.
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