Papers by Sunil K Rastogi
Indian Journal of Animal Research, 2015
The present study has demonstrated the expression of HSP70-1 gene in bovine peripheral blood mono... more The present study has demonstrated the expression of HSP70-1 gene in bovine peripheral blood mononuclear cells (PBMCs). Blood samples were collected aseptically by jugular vein puncture in EDTA (1mg/ml) from six apparently healthy sahiwal cows (Bos Indicus). Peripheral blood mononuclear cells (PBMCs) were separated from the blood using HiSep TM Lymphocyte Separation Media (LSM). Total RNA was isolated from PBMCs using Trizol reagent. The purity and concentration of total RNA was checked using Nanodrop instrument whose further confirmation was made by using denaturing agarose gel (1.5%) electrophoresis. Isolated total RNA was reverse transcribed to cDNA that was used for amplification of HSP70-1 gene. Full length open reading frame (ORF) of HSP70-1 gene was amplified using specific primers pair. The presence of 1926 base pair band in denaturing agarose indicated the amplification of HSP70-1 gene. To conclude, our results demonstrate that HSP70-1 gene is expressed in bovine PBMCs.
Research Square (Research Square), Jul 21, 2022
The present investigation was undertaken to study the effect of different stages of lactation on ... more The present investigation was undertaken to study the effect of different stages of lactation on erythrocyte osmotic fragility, blood leucocyte indices and milk somatic cell count in indigenous Badri cattle. Badri is rst registered indigenous, dual purpose cattle breed of the state Uttarakhand (India) mainly reared in hilly areas of the state. Osmotic fragility evaluates the stability of erythrocyte membrane to osmotic stress. Leucocytes form defence mechanism against invading microorganisms. Somatic cell count is an indicator of udder health. The study was carried out in forty healthy Badri cows divided into ve groups according to their lactation stages viz. group 1 (0-60 days), group 2 (61-120 days), group 3 (121-180 days), group 4 (181-240 days) and group 5 (to be dried off). Erythrocyte osmotic fragility, blood total leucocyte count (TLC), differential leucocyte count (DLC) and milk somatic cell count (SCC) were estimated. The percent hemolysis of erythrocytes among different groups was signi cant (p < 0.05) at 0.9% and 0.5% saline concentrations. A signi cant effect (p < 0.05) of lactation stages on blood lymphocytes, neutrophils and milk somatic cell count was observed.
Tuberculosis is one of the oldest diseases with a death rate of 1.5 million per year. Tuberculosi... more Tuberculosis is one of the oldest diseases with a death rate of 1.5 million per year. Tuberculosis spreads from one person to another through Mycobacterium tuberculosis. This bacteria belongs to the family Mycobacteriaceae, genus Mycobacterium, member of the tuberculosis complex. Mycobacterium tuberculosis is an acid-fast, aerobic, rod-shaped bacteria, ranging from 2 to 4 Â lm in length and 0.2 to 0.5 Â lm in width. Tuberculosis spreads through infected people via sneezing, coughing, etc., with humans acting as the host for the bacteria. The genome of Mycobacterium tuberculosis H37Rv encodes 3906 proteins, of which 1055 are hypothetical proteins (HPs), wherein the functions of the proteins are unknown. The sequences of 1055 HPs of Mycobacterium tuberculosis were analyzed and the functions of 578 HPs were subsequently predicted with a high level of confidence. Several enzymes, transporters and binding proteins of 1055 HPs in M. tuberculosis were analyzed and potential targets were discovered which contribute to the overall survival of the bacteria. The analysis will be of relevance in understanding the mechanism of the bacteria and will prove to be beneficial in the discovery of new drugs.
Indian Journal of Small Ruminants, 2007
Indian Journal of Animal Sciences, Oct 17, 2006
Metabolic profile of dairy cattle maintained under farm 1 (R-l, n=65), farm 2 (R-2, n=70) and nom... more Metabolic profile of dairy cattle maintained under farm 1 (R-l, n=65), farm 2 (R-2, n=70) and nomadic dairy herd of Dehradun h1lls .(R-3, n=65) regions of Uttaranchal state, were studied involving serum biochemical and enzymatic parameters. Animals under first 2 regions were stall fed with dry or green fodder supplemented with concentrate, whereas, third herd was totally thriving on grazing. Decreasing trend of nonsignificant nature was recorded in overall mean serum glucose, protein, albumin, cholesterol and urea concentrations with highest level in R-I, followed by R-2 and R-3. These changes reflected dietary status and relative utilization of energy and nitrogen by these cattle. Glucose was significantly lower (55.2±2.14 mg/dl) in grazing pubertal heifers, and higher in early lactation cows of all the regions. Serum protein concentration showed variable trend but characteristically higher (P~O.05) albumin concentrations were recorded in most categories of R-l, indicating greater reliability of these parameters towards nitrogen retention in dairy cattle. Serum cholesterol levels also paralleled to glucose and albumin whereas urea concentration exhibited variations of much lower magnitude. Significantly higher serum alkaline phosphatase activity was recorded in young calves of all 3 regions with respective values of27J.4±15.59, 280.0±7.84 and 301.4±17.81 U/1. No such trend was visible for various other categories and also for transaminase activities. The data suggested applicability of serum glucose, albumin and cholesterol concentrations for assessing nutritional status of dairy cattle.
Indian Journal of Small Ruminants, 2005
Indian Journal of Animal Research, 2007
The present study was conducted to observe the effect of light source and different photoperiods ... more The present study was conducted to observe the effect of light source and different photoperiods on the growth rate and feed efficiency in broiler chicks during February to March at Pantnagar. Initially, the day old chicks in group “A” (n= 288) were exposed to fluorescent light of 30–40 Lux and chicks in group “B” (n= 283) were exposed to incandescent light of 120–150 Lux for 24 h continuous light during first week of experiment. In the 2ndand 3rdweek, they were exposed to 23 h Light (LL) and 1 h Dark (DD) (LL 23: DD 1). In the 4th and 5th week, birds from group “A” were divided into four groups (n= 65 each) and exposed to LL 23: DD 1, LL 20: DD 4, LL 18: DD 6 at 15–20 Lux and natural photoperiod of LL 13: DD 11 at 50–70 Lux. The results indicated that the fluorescent light of 30–40 Lux during first three weeks of experiment showed significant increment in growth rate and improve feed conversion efficiency compared to the birds maintained in the incandescent light of 120–150 Lux. The last three weeks experiment indicated that, the 4–6 h of dark phase helps to improve the production performance in birds compared to 1 h or 11 h dark phase.
Indian Journal of Animal Sciences, Oct 5, 2005
Female buffalo calves (4) were studied for renal clearance of endogenous and exogenous (creatinin... more Female buffalo calves (4) were studied for renal clearance of endogenous and exogenous (creatinine infused @ 15.0 mg/ kg and 40.0 mg/kg body wt) creatinine clearance and compared with inulin clearance. Both dose rates provided better estimates with respective values of glomerulal filtration rates (GFR) 187.7±9.0, 211.O±6.6 ml/min. Respective values for endogenous creatinine and insulin clearance were 203.0±8.8 and 180.1±7.0 ml/min. Creatinine clearance at low dose rate level gave better significant positive correlation (r=0.845)compared to higher dose rate level (r=0.187),which was non-significant. Data indicated that infusion of exogenous creatinine at 15.0 mg/kg body weight could be used to assess glomerular filtration rate in buffalo calves in place of more tedious and cumbersome inulin clearance.
Indian journal of animal nutrition, 2005
An experiment was conducted to asses the effect of replacement of oat straw with graded levels of... more An experiment was conducted to asses the effect of replacement of oat straw with graded levels of green berseem on the nutrient availability. The In-sacco degradability of oat straw: green berseem combinations (80: 20, 70: 30, 60: 40 and 50:50 ratio on DM basis) revealed that the effective degradabiilty of DM increased (P<0.05) linearly and the undegradable fraction declined (P<0.05) with the increase in level of berseem. A combination of oat straw and berseem in 50: 50 ratio gave the best results. The in-vivo evaluation of these combinations on 16 crossbred heifers revealed that the DM intake (as % BW) was higher (P<0.05) in combination containing 50% berseem as compared to the one containing only 20 percent berseem, whereas no significantly difference was observed between other treatmetns. The digestibility of nutrients incerased linearly. The digestibility of DM, CP and NDF was statistically comparable in the combinations containing 40 or 50% berseem but significantly (P<0.05) higher than other combina-tions. It was concluded that oat straw and green berseem in 60:40 ratio resulted in optimum nutrient utilization in heifers.
Indian Journal of Animal Sciences, 1990
International Journal of Farm Sciences, 2022
Indian journal of poultry science, 2005
Commercial broiler chicks divided into four groups (18 chicks each) received virginiamycin @ 0 (c... more Commercial broiler chicks divided into four groups (18 chicks each) received virginiamycin @ 0 (control), 5, 10 and 20 mg (treatment) per kg feed from first week through 7 week of age. Final body weight recorded was significantly (P<0.05) higher in all the treatment groups (1465, 1455 and 1490 g respectively) compared to the control birds (1351 g). Feed conversion ratio was also superior (P<0.05) in experimental groups (2.35, 2.36 and 2.25 respectively) compared to the controls (2.47). Weights of liver, gizzard, spleen and length of intestines and femur were significantly (P<0.05) higher in third experimental group. PCV, Hb and total plasma protein concentrations were significantly lower in all three experimental groups compared to controls indicating a favourable nutrient partitioning influence. Virginiamycin supplementation also resulted in improved carcass quality of the birds under study. Among various dose rates used, 20 mg/kg of feed supplementation produced best growth promoting effect.
Buffalo Bulletin, 2014
Liquid phase blocking (LPB) ELISA is approved for the screening of sera samples after vaccination... more Liquid phase blocking (LPB) ELISA is approved for the screening of sera samples after vaccination and non-structural protein (NSP) ELISA for confi rmation of infection regardless of vaccination status of animals. In the present study sera samples from 300 buffalo were used to screen for both protective and NSP antibody titre against foot-and-mouth disease (FMD) virus. Overall, 6.67% buffalo were infected with FMD whereas 10.67% had suspicion level of titre against the disease. However, protective antibody titre against O, A and Asia-1 serotypes were respectively, 62.67%, 20.00% and 48.00%. Among the breeds, a greater number of Murrah had protective antibody titre against different serotypes of FMD virus (FMDV) namely O, A and Asia-1 than other breeds of buffalo. Comparison of protective antibody titre and doubtful titre of NSP antibody between male and female showed that the proportion of female buffaloes had higher in both protective antibody and titre against NSP in doubtful status. However, no cases of infection were detected in male animals but 9.62% female buffalo were infected with FMD. Similarly, in the age groups of 0-<4 years and ≥8 years, no cases of FMD infection were established, but 8.20% buffaloes in the age group of 4-<8 years was infected. Proportion of buffaloes that had protective antibody titre was highest in the age group of 0-<4 years followed by 4-<8 years and ≥8 years. Breed, sex and age of buffaloes infl uenced the protective antibody titre (p<0.01) against different serotypes of FMDV except for serotype A in breeds and age groups of animals (P>0.01).
Tropical Animal Health and Production, Nov 9, 2019
Infectious Bursal Disease (IBD) is an economically important, immunosuppressive viral disease of ... more Infectious Bursal Disease (IBD) is an economically important, immunosuppressive viral disease of chicken. Withania somnifera, a well-known Indian medicinal plant and functional food, finds extensive ethnomedicinal and ethnoveterinary use in the subcontinent. Root extracts of Withania somnifera have been shown to inhibit IBD virus (IBDV) in vitro. The effect of dietary supplementation with whole root powder of Withania somnifera was studied in chicken experimentally infected with IBDV. Dietary supplementation with the root powder improved erythrocytic indices, biochemical parameters, bursal weight index, and lymphocyte stimulation indices, and reduced histopathological insult in the infected birds. Viral load decreased to less than onefourth in the birds receiving dietary supplementation with Withania somnifera root powder. It could be concluded that continued supplementation of IBDV-infected chicken with Withania somnifera root powder alleviated virus-induced stress and histological and immunological alterations and reduced viral persistence in the host.
Journal of Veterinary Medicine and Animal Health, Apr 30, 2010
A comparative study on antistressor and antioxidative effects of synthetic vitamin C and polyherb... more A comparative study on antistressor and antioxidative effects of synthetic vitamin C and polyherbal feed premix supplementation in broilers was conducted during the summer months of June-July when the mean temperature-humidity index was 84.74 ± 2.51. Day old broiler chicks (n = 60) were randomly divided into three groups. Control group I was given basal diet and treatment groups (II and III) were supplemented with synthetic vitamin C (100 g/tonne of feed) and polyherbal feed premix (1 kg/tonne of feed) from day 0 to 6 weeks of age. Biochemical parameters were analysed after the 3 rd and the 5 th week and erythrocytic antioxidant enzymes were analysed after the 3 rd and the 6 th week of experiement. Hormonal and immunological parameters were analysed after the 6 th week of the study. After the 3 rd week, mean plasma glucose, cholesterol and antioxidant enzyme glutathione reductase (GSSG) were significantly (P ≤ 0.01) lower in treated groups (II and III) than control (I); however total protein, albumin to globulin ratio and antioxidant enzyme superoxide dismutase (SOD) were significantly (P ≤ 0.05) different in group II and III compared to group I. After the 5 th week, mean plasma glucose, total protein, albumin globulin ratio were significantly (P ≤ 0.05) different in both the treatments compared to control. Erythrocytic GSSG were significantly (P ≤ 0.05) different in both the treatments than control, as observed after the 6 th week. Stress hormones namely cortisol and thyroxine (T 4) were observed to be significantly (P ≥ 0.05) higher in the untreated controls than the treated groups. Mean total immunoglobulin (Ig) level was significantly (P ≥ 0.01) higher in polyherbal premix and vitamin C treated birds than control birds after the 6 th week of study. It can be concluded from the results that oxidative stress in broilers during summer could be ameliorated using antioxidant synthetic vitamin C and the polyherbal antistressor, immunomodulator and adaptogenic feed premix.
International Journal of Basic and Applied Agricultural Research, 2015
Six apparently healthy, non-lactating and non-pregnant Sahiwal cows and Murrah buffalo each above... more Six apparently healthy, non-lactating and non-pregnant Sahiwal cows and Murrah buffalo each above two years of age were selected to evaluate the effect of seasonal variations on hematological parameters. Blood samples were collected aseptically by jugular vein puncture during peak winter and peak summer seasons. The present investigation revealed the mean values of Hb, PCV, TEC, neutrophils, MCH, MCHC were significantly higher (P 0.05). The neutrophils/lymphocyte ratio was significantly higher (P<0.05) during summer as against the winter season in case of Murrah buffalo, whereas no significant alternation was registered in case of Sahiwal cows. These findings suggested that Murrah buffalo are more prone to stress due to seasonal variations in comparison to Sahiwal cows.
Indian Journal of Animal Research, 2013
A 21 days trial was conducted to assess the effect of extensive, intensive and semi-intensive fee... more A 21 days trial was conducted to assess the effect of extensive, intensive and semi-intensive feeding practices on electro-encephalographic (EEG) recordings in six female goats (Local X Jamunapari). The study was done in three phases, phase I (GZ) comprised of extensive feeding, phase II (STF) consisted of intensive feeding and phase III (GZ+STF) consisting of semi-intensive feeding. An EEG recording was done daily in morning before grazing/feeding and in afternoon after grazing/feeding using bipolar lead system (RO-LO). The recordings of EEG amplitude and frequency for all three phases of experiment ranged from 0.95 (phase II) to 2.07 mV (phase III) and 10.50 (phase III) to 20.22 Hz (phase II) , respectively. Goats in semi-intensive feeding practice showed d wave pattern, whereas low b wave pattern was exhibited in extensive and intensive system of management. The electroencephalographic recordings can be implemented to evaluate the neuro-motor activity in goats subjected to different feeding practices.
Small Ruminant Research, 2002
Of eight clinically endemic goitrous goats procured locally, six were treated with sodium thyroxi... more Of eight clinically endemic goitrous goats procured locally, six were treated with sodium thyroxine at 0.2 mg per day per goat orally for 100 days. Histopathology of heart in two untreated and two treated goats revealed extensive fatty vacuolization of myocardial ®bers in the former group, which was replaced by granularity in the latter group. There was higher plasma total cholesterol and lipids in goitrous goats (
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Papers by Sunil K Rastogi