Papers by Clementina O Adenipekun
Crude oil polluted soils of different concentrations (0%,1%,5%,10%,20%,30% and 40%) were inoculat... more Crude oil polluted soils of different concentrations (0%,1%,5%,10%,20%,30% and 40%) were inoculated and incubated with P.pulmonarius and P.ostreatus for 0,1 and 2 months to study and compare their effect in the bioremediation of crude oil and bioaccumulation of heavy metals from polluted soils. Nutrient contents of the soil were determined on monthly basis. Also, the heavy metals accumulation by the fungi from the polluted soil was monitored. Results showed that both white rot fungi were able to biodegrade and ameliorate the soils by increasing the nutrient contents as the concentration of the crude oil in the soils increases with increase in incubation period. Highest increase in concentration of these nutrient contents were obtained at 40% crude oil contaminated soils after 2 months incubation period. Organic matter was the highest nutrient content recorded followed by organic carbon, potassium, nitrogen, and phosphorus in decreasing order. P.pulmonarius had the highest nutrient content than P.ostreatus having 32.60% and 30.46% organic matter; 18.91% and 17.67% organic carbon, 1.96% and 1.85% nitrogen; 13.50mg/kg and 12.60mg/kg phosphorus and 1.91cmol/kg and 1.70cmol/kg potassium respectively. The pH values reduced for both white rot fungi after inoculation into crude oil contaminated soils. P.pulmonarius had the highest pH values of 6.50 and 6.40 at 0% crude oil polluted soil for 1 and 2 months and lowest pH values of 4.70 and 4.80 at 40% and 10% crude oil contaminated soils. While P.ostreatus had the highest value of 5.97 and 6.17 at 0% and lowest pH of 4.63 and 5.27 at 30% and 40% crude oil contaminated soils. Heavy metal accumulation increases as the concentration of crude oil increased and decreased as the incubation period increased from 1 to 2 months for both fungi. Iron (1.26mgkg -1 at 40% crude oil concentration) was the highest heavy metal accumulated by P.pulmonarius after one month while copper (2.28mgkg -1 at 40% crude oil concentration) was the highest heavy metal accumulated by P.ostreatus for the same period. Nickel was the least heavy metal accumulated. The result obtained showed the ability of P.pulmonarius and P.ostreatus to bioremediate a hydrocarbon and heavy metal polluted soil. [Adenipekun C. O., Olanrewaju, O.O and Ogunjobi, A.A. Bioaccumulation of Heavy metals and Nutrient content Supplementation by two White rot fungi in Crude oil polluted soils. Researcher. 2011;3(5):13-20]. (ISSN: 1553-9865). http://www.sciencepub.net.
hydrocarbons has detrimental effects on ecosystems. As such contaminants constitute risk to human... more hydrocarbons has detrimental effects on ecosystems. As such contaminants constitute risk to human health; they can enter the food chain through agricultural products or contaminated drinking water. This growing concern about contamination with cutting fluids makes remediation process imperative. White rot fungus, Pleurotus pulmonarius was investigated in this pilot study for its potential to bioremediate contaminants such as heavy metals and total petroleum hydrocarbon at 10, 20 and 30% contamination levels. Control experiment (0% contamination level) was also set up. The effects of incubation periods on the contents of cutting fluid contaminated soil inoculated with P. pulmonarius after two months was equally studied. Bioremediation efficiently occurred at 10% contamination level. At this level, soil pH was 6.9 and organic carbon, organic matter and phosphate contents increased by 22.4, 21.4 and 3.5%, respectively for spent cutting fluid and by 30.0, 30.1 and 50.0% for fresh cutting fluid respectively after two months of incubation with the fungus. Copper, manganese and nickel decreased significantly by 35.3, 25.0 and 1.5%, respectively for spent cutting fluid contaminated soil and by 10.5, 54.4 and 55.9% respectively for fresh cutting fluid contaminated soil. High percentage degradation of Total Petroleum Hydrocarbon (TPH) at 10% contamination level was about 90.0% for both fresh and spent contaminated soil samples. The cation exchange capacity increased by 50.8 and 38.4% for spent and fresh cutting fluid contaminated soil samples respectively. These results indicate the efficiency of P. pulmonarius as a bioremediating agent of heavy metals and TPH in contaminated soil.
The Environmentalist, 2008
The effect of spent engine oil on the height, leaf number, leaf area, stem girth, chlorophyll, an... more The effect of spent engine oil on the height, leaf number, leaf area, stem girth, chlorophyll, and moisture contents of Corchorus olitorius grown on 0, 0.2%, 0.4%, 0.6%, 0.8%, 1.0%, 2.0%, and 3.0% (v/w oil/soil) oil-contaminated soil was investigated. The engine oil at all concentrations delayed the germination of C. olitorius by 2 days (compared to control) and there was a general significant reduction in all the growth parameters in plants grown on contaminated soil compared to control plants. The highest leaf area of 26.8 cm2 was found in the control plant and least was found in the 0.6% soil (0.11 cm2) after 3 weeks while no values were recorded on the 0.8–3.0% engine-oil-contaminated soil after 5 weeks of experiment. The highest chlorophyll content was also found in the control plant (11.5 mg/l). This showed that spent engine oil has an adverse effect on the growth of C. olitorius plant.
International Journal of Medicinal Mushrooms, 2010
ABSTRACT ABSTRACT: Lentinus squarrosulus Mont. is an important tropical edible and medicinal mush... more ABSTRACT ABSTRACT: Lentinus squarrosulus Mont. is an important tropical edible and medicinal mushroom distributed across Sub-Saharan Africa and most parts of Asia. Intrastock mating study was performed using single spore isolates (ssi) from a wild strain (MBFBL 201). Mycelia growth and morphology characterization indicated that milled corn straw agar (CSA) medium best supports mycelia growth compared to two other media: potato dextrose agar (PDA) and yeast peptone soluble starch agar (YPSS). The results from ssi pairings and analysis confi rmed a tetrapolar mating system for L. squarrosulus. Further characterization of the resulting dikaryons indicated that the growth rate of the parent monokaryons does not affect the growth rate of the resulting dikaryon. Primordial formation in the resulting dikaryons with the fastest growth rate was 12 days after inoculation. Under similar conditions, MBFBL 201 and another wild strain, MBFBL 269, produced primordia after 28 and 32 days, respectively, indicating that intrastock breeding could be an effective method for obtaining improved strains of L. squarrosulus. Further characterization to test growth and performance in order to obtain high-yield strains for use in commercial cultivation is ongoing.
Pakistan Journal of Biological Sciences, 2008
This study was conducted to test the efficacy of an indigenous white rot fungus Lentinus squarros... more This study was conducted to test the efficacy of an indigenous white rot fungus Lentinus squarrosulus in degrading engine oil in soil. Flasks containing sterilized garden soil (100 g) moistened with 75% distilled water (w/v) were contaminated with engine oil 1, 2.5, 5, 10, 20 and 40% w/w concentrations, inoculated with L. squarrosulus and incubated at room temperature for 90 days. Levels of organic matter, pH, total hydrocarbon and elemental content (C, Cu, Fe, K, N, Ni, Zn and available P) were determined post-fungal treatment. Results indicate that contaminated soils inoculated with L. squarrosulus had increased organic matter, carbon and available phosphorus, while the nitrogen and available potassium was reduced. A relatively high percentage degradation of Total Petroleum Hydrocarbon (TPH) was observed at 1% engine oil concentration (94.46%), which decreased to 64.05% TPH degradation at 40% engine oil contaminated soil after 90 days of incubation. The concentrations of Fe, Cu, Zn and Ni recovered from straw/fungal biomass complex increased with the increase of engine-oil contamination and bio-accumulation by the white-rot fungus. The improvement of nutrient content values as well as the bioaccumulation of heavy metals at all levels of engine oil concentrations tested through inoculations with L. squarrosulus is of importance for the bioremediation of engine-oil polluted soils.
Objectives: To investigate the potential of Pleurotus pulmonarius in the bioremediation of soil c... more Objectives: To investigate the potential of Pleurotus pulmonarius in the bioremediation of soil contaminated with spent diesel oil at 5, 10 and 15% (v/w) levels) of contamination over a period of one and two months of incubation. Methodology and results: A pure culture of P. pulmonarius was obtained from the Plant physiology unit of the Department of Botany; University of Ibadan Two sets of control were used. In the first set of control treatments, no contaminant (spent diesel oil) was added to the soils while in the second set, different levels of the oil were added to all soils but no inoculation with the fungus). Inoculation was done by adding 10 g of vigorously growing spawn of P. pulmonarius. The soil was contaminated with spent diesel oil to achieve 5 %, 10 % and 15 % (w/v). The results showed a significant increase (at p≤ 0.05) in the nutrient contents of the soil samples at all levels of contamination compared to the uncontaminated but there was no significant difference in the pH after two months of incubation with P. pulmonarius. The organic carbon increased from 2.176 to 6.960 at 0% and from 7.126 to 8.010 at 15 % level of contaminations. Organic matter increased from 3.765 to 12.03 at 0% level of contamination and from 12.32 to 13.84 at 15%. Phosphorus increased from 15.807 to 18.940 at 0% and 16.017 to 20.627 at 5% level of contaminations while Potassium increased from 0.660 to 0.783 at 0% and 0.540 to 0.837 at 10% level of contamination. The Total Petroleum Hydrocarbon (TPH) degradation (% loss) was 84.41% at 5%, 64.87% at 10% and 44.27% at 15% level of contamination. . A significant (p ≤0.05) reduction in concentration of the heavy metals Cu and Zn after two months of incubation with P. pulmonarius suggested bioaccumulation. The Copper content reduced from 0.033 to 0.014mg/kg at 0% oil contamination and from 0.027 to 0.021mg/kg at 15% oil contamination while Zinc reduced from 0.076 to 0.059mg/kg at 0% oil, and from 0.057 to 0.056mg/kg at 15% oil contamination. There was an improvement in the growth of Corchorus olitorius in the remediated soil at all levels of contamination compared to the control. Plant growth in the remediated soil was 11.18 cm high after the sixth week while the control plant was 4.23 cm at 5% level contamination. Conclusion and application: The fungus P.pulmonarius has been found from this study to be a potential white rot fungus that can bioremediate soil contaminated with diesel oil and that the remediated soil was able to support the growth of the test plant at all levels of contamination. The biotechnology is a cost effective energy input that can also fetch the farmer some income.
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Papers by Clementina O Adenipekun