Papers by Anna Carolina Marinho
Anais do I Simpósio Internacional de Imunobiológicos: os atuais desafios para a construção de embiente favorável à inovação e ao desenvolvimento tecnológico, 2013
Journal of Virological Methods
As meningites bacterianas continuam sendo um grande problema de saude publica no Brasil e no mund... more As meningites bacterianas continuam sendo um grande problema de saude publica no Brasil e no mundo, constituindo importante causa de morbimortalidade na infância. Em todo o mundo, sem epidemias, ocorre um milhao de casos de meningite bacteriana por ano. Estas doencas sao quase sempre emergencias medicas e estao associadas a elevadas taxas de mortalidade, dai a preocupacao (e a publicidade) a que estao habitualmente associadas.
Anais do XX Congresso Brasileiro de Engenharia Química, 2015
International Journal of Biological Macromolecules, 2021
Given the magnitude of the global snakebite crisis, strategies to ensure the quality of antivenom... more Given the magnitude of the global snakebite crisis, strategies to ensure the quality of antivenom, as well as the availability and sustainability of its supply are under development by several research groups. Recombinant DNA technology has allowed the engineering of monoclonal antibodies and recombinant fragments as alternatives to conventional antivenoms. Besides higher therapeutic efficacy, with broad neutralization capacity against local and systemic toxicity, novel antivenoms have to ensure safety and cost-effectivity. Due to the biological and physical chemical properties of camelid single-domain antibodies, with high volume of distribution to distal tissue, their modular format, and versatility, their biotechnological application has grown considerably in recent decades. This article presents the most up-to-date developments concerning camelid single-domain-based antibodies against major toxins from snake venoms, the main venomous animals responsible for reported envenoming cases and related human deaths. A brief discussion on the constitution, challenges, and perspectives of antivenoms is presented, as well as the road ahead for next-generation antivenoms based on single-domain antibodies.
Molecular Diagnosis & Therapy, 2021
The distinct biophysical and pharmaceutical properties of camelid single-domain antibodies, refer... more The distinct biophysical and pharmaceutical properties of camelid single-domain antibodies, referred to as VHHs or nanobodies, are associated with their nanometric dimensions, elevated stability, and antigen recognition capacity. These biomolecules can circumvent a number of diagnostic system limitations, especially those related to the size and stability of conventional immunoglobulins currently used in enzyme-linked immunosorbent assays and point-of-care, electrochemical, and imaging assays. In these formats, VHHs are directionally conjugated to different molecules, such as metallic nanoparticles, small peptides, and radioisotopes, which demonstrates their comprehensive versatility. Thus, the application of VHHs in diagnostic systems range from the identification of cancer cells to the detection of degenerative disease biomarkers, viral antigens, bacterial toxins, and insecticides. The improvements of sensitivity and specificity are among the central benefits resulting from the use of VHHs, which are indispensable parameters for high-quality diagnostics. Therefore, this review highlights the main biotechnological advances related to camelid single-domain antibodies and their use in in vitro and in vivo diagnostic approaches for human health.
Vaccines are important instruments for public health. Sanitary registration is an important tool ... more Vaccines are important instruments for public health. Sanitary registration is an important tool for the national regulatory authorities to control the medicines use and it is the basic source of information for the National Sanitary Surveillance System – NRAs. For meningococcal meningitis, caused by Neisseria meningitidis (Nm), many vaccines were developed against serogroups A, C. W135, and Y using the capsular polysaccharides. Considering that the serogroup B polysaccharide is poorly immunogenic in human, other vaccines strategies were tried. This study aimed at identifying the applied patents for meningitis B antigens during the period from 1990 to 2005, and these were compared to the registered products. The European Patent Office and the National Institute for Industrial Propriety data basis were used to survey the patents request. Several patents requested the antigens safekeeping, which are included into the registered vaccine, and it does not characterize an innovation. The ...
1. Introdução Os interferons (IFNs) são citocinas e são utilizados como medida terapêutica contra... more 1. Introdução Os interferons (IFNs) são citocinas e são utilizados como medida terapêutica contra a infecção de vírus e outras doenças devido tanto à sua atividade antiviral quanto imunomodulatória. Os IFN-hr-α-2a e IFN-hr-α-2b são proteínas bioterapêuticas não glicosiladas contendo 165 aminoácidos e diferindo apenas pela presença de uma lisina e arginina na posição 23, respectivamente. Possuem duas pontes de dissulfeto e massa molar de aproximadamente 19,3 kDa. Na farmacopeia brasileira (FB) não existe monografia para este tipo de produto somente recomendações de ensaios gerais, por outro lado na farmacopeia europeia (FE) encontra-se monografia para o IFN-α, porém somente para matéria-prima, sendo recomendado os seguintes ensaios físico-químicos: focalização isoelétrica, mapa de peptídeos e SDS-PAGE.
Vigilância Sanitária em Debate, 2018
Anais do I Simpósio Internacional de Imunobiológicos: os atuais desafios para a construção de embiente favorável à inovação e ao desenvolvimento tecnológico, 2013
Revista do Instituto Adolfo Lutz
Vaccines are important instruments for public health. Sanitary registration is an important tool ... more Vaccines are important instruments for public health. Sanitary registration is an important tool for the national regulatory authorities to control the medicines use and it is the basic source of information for the National Sanitary Surveillance System – NRAs. For meningococcal meningitis, caused by Neisseria meningitidis (Nm), many vaccines were developed against serogroups A, C. W135, and Y using the capsular polysaccharides. Considering that the serogroup B polysaccharide is poorly immunogenic in human, other vaccines strategies were tried. This study aimed at identifying the applied patents for meningitis B antigens during the period from 1990 to 2005, and these were compared to the registered products. The European Patent Office and the National Institute for Industrial Propriety data basis were used to survey the patents request. Several patents requested the antigens safekeeping, which are included into the registered vaccine, and it does not characterize an innovation. The majority of the patents depositories belong to multinational companies and the Brazilian institutions should put in practice the process of requesting the patent for their research products.
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Papers by Anna Carolina Marinho